Rabbit Recombinant Monoclonal PD-L2 antibody. Carrier free. Suitable for IP, WB, Flow Cyt (Intra) and reacts with Human samples.
Images
![Immunoprecipitation - Anti-PD-L2 antibody [CAL28] - BSA and Azide free (AB261838), expandable thumbnail](https://content.abcam.com/products/images/pd-l2-antibody-cal28-bsa-and-azide-free-ab261838--immunoprecipitation-img3601.jpg/_jcr_content/renditions/webp-475.webp "Immunoprecipitation - Anti-PD-L2 antibody [CAL28] - BSA and Azide free (AB261838)")
![Flow Cytometry (Intracellular) - Anti-PD-L2 antibody [CAL28] - BSA and Azide free (AB261838), expandable thumbnail](https://content.abcam.com/products/images/pd-l2-antibody-cal28-bsa-and-azide-free-ab261838--flow-cytometry-intracellular-img414765.jpg/_jcr_content/renditions/webp-475.webp "Flow Cytometry (Intracellular) - Anti-PD-L2 antibody [CAL28] - BSA and Azide free (AB261838)")
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: PBS- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| IP | WB | IHC-P | ICC/IF | Flow Cyt (Intra) | |
|---|---|---|---|---|---|
| Human | Tested | Expected | Not recommended | Not recommended | Tested |
IP
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
WB
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info Use at an assay dependent concentration. | Notes - |
IHC-P
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes Although this clone performed well in IHC-P on tonsil tissue, non-specific nuclear staining was seen on TMA. The staining pattern did not correlate with ISH, so we cannot recommend this clone for IHC. Both EDTA buffer pH9 and citrate buffer pH6 have been used successfully. Please refer to figure legends. Perform heat-mediated antigen retrieval before commencing with IHC staining protocol. |
ICC/IF
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
Flow Cyt (Intra)
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
Associated Products
Select an associated product type
1 product for Alternative Product
7 products for Alternative Version
Target data
Function
Involved in the costimulatory signal, essential for T-cell proliferation and IFNG production in a PDCD1-independent manner. Interaction with PDCD1 inhibits T-cell proliferation by blocking cell cycle progression and cytokine production (By similarity).
Alternative names
CD273, B7DC, PDCD1L2, PDL2, PDCD1LG2, Programmed cell death 1 ligand 2, PD-1 ligand 2, PD-L2, PDCD1 ligand 2, Programmed death ligand 2, Butyrophilin B7-DC, B7-DC
Recommended products
Rabbit Recombinant Monoclonal PD-L2 antibody. Carrier free. Suitable for IP, WB, Flow Cyt (Intra) and reacts with Human samples.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: PBS- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Carrier free
- Yes
- Clone number
- CAL28
- Purification technique
- Affinity purification Protein A
- Specificity
Although this clone performed well in IHC-P on tonsil tissue, non-specific nuclear staining was seen on TMA. The staining pattern did not correlate with ISH, so we cannot recommend this clone for IHC.
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- +4°C
- Storage information
- Do Not Freeze
Notes
ab261838 is the carrier-free version of Anti-PD-L2 antibody [CAL28] ab256386.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
PD-L2 also known as PDCD1LG2 or B7-DC is a member of the B7 protein family that plays a role in immune system regulation. This protein has a molecular mass of approximately 25 kDa. PD-L2 is expressed in antigen-presenting cells such as dendritic cells and macrophages. Expression is also located in some subsets of T cells and B cells. The PD-L2 can act as a second ligand for the PD-1 receptor which is important in immune checkpoints.
- Biological function summary
PD-L2 offers protection to tissues from autoimmunity by regulating T-cell activation and fostering tolerance. By engaging with its receptor PD-1 PD-L2 forms a complex that modulates the immune response to prevent overactivation. This interaction limits the immune system's potential damage to healthy tissues maintaining proper immune function in diverse environments.
- Pathways
PD-L2 participates in the immune checkpoint pathways where it works closely with PD-1 to mediate immune responses. PD-L2 forms an important part of the program death-1 (PD-1) pathway which plays a prominent role in downregulating T-cell activity. It relates to proteins such as PD-L1 which also interacts with PD-1 modulating immune tolerance and response to infections or tumors.
- Associated diseases and disorders
PD-L2 involvement has been significant in cancer immunology and autoimmune conditions. Its interaction with PD-1 relates to the suppression of immune responses in tumors contributing to cancer progression. PD-L2's function as an immune checkpoint ligand associates with autoimmune diseases like rheumatoid arthritis where immune regulation goes awry. Both PD-1 and PD-L2 represent potential targets for therapeutic interventions due to their roles in these conditions.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
2 product images
![Immunoprecipitation - Anti-PD-L2 antibody [CAL28] - BSA and Azide free (ab261838), expandable thumbnail](https://content.abcam.com/products/images/pd-l2-antibody-cal28-bsa-and-azide-free-ab261838--immunoprecipitation-img3601.jpg "Immunoprecipitation - Anti-PD-L2 antibody [CAL28] - BSA and Azide free (ab261838)")
Immunoprecipitation - Anti-PD-L2 antibody [CAL28] - BSA and Azide free (ab261838)
PD-L2 was immunoprecipitated from 0.35 mg of HDLM-2 (human Hodgkin lymphoma) whole cell lysate with Anti-PD-L2 antibody [CAL28] ab256386 at 1/30 dilution. Western blot was performed from the immunoprecipitate using ab256386X at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (VeriBlot for IP Detection Reagent (HRP) ab131366), was used as secondary antibody at 1/5000 dilution.
This data was developed using the same antibody clone in a different buffer formulation containing PBS and sodium azide (Anti-PD-L2 antibody [CAL28] ab256386).
All lanes: Immunoprecipitation - Anti-PD-L2 antibody [CAL28] (Anti-PD-L2 antibody [CAL28] ab256386)
Predicted band size: 31 kDa
Observed band size: 45-60 kDa
![Flow Cytometry (Intracellular) - Anti-PD-L2 antibody [CAL28] - BSA and Azide free (ab261838), expandable thumbnail](https://content.abcam.com/products/images/pd-l2-antibody-cal28-bsa-and-azide-free-ab261838--flow-cytometry-intracellular-img414765.jpg "Flow Cytometry (Intracellular) - Anti-PD-L2 antibody [CAL28] - BSA and Azide free (ab261838)")
Flow Cytometry (Intracellular) - Anti-PD-L2 antibody [CAL28] - BSA and Azide free (ab261838)
Intracellular flow cytometric analysis of 4% paraformaldehyde-fixed, 90% methanol-permeabilized HDLM-2 (human Hodgkin lymphoma) cells labeling PD-L2 with Anti-PD-L2 antibody [CAL28] ab256386 at 1/500 dilution (Red) compared with a Rabbit monoclonal IgG Isotype control (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) (Black) and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). Goat Anti-Rabbit IgG H&L (Alexa Fluor®488) (Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077), at 1/2000 dilution was used as the secondary antibody.
This data was developed using the same antibody clone in a different buffer formulation containing PBS and sodium azide (Anti-PD-L2 antibody [CAL28] ab256386).
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