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Mouse Recombinant Monoclonal PD-L2 antibody. Suitable for IP, WB and reacts with Human samples.

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Images

Western blot - Anti-PD-L2 antibody [CAL28] – Mouse IgG1 (Chimeric) (AB283344), expandable thumbnail
  • Immunoprecipitation - Anti-PD-L2 antibody [CAL28] – Mouse IgG1 (Chimeric) (AB283344), expandable thumbnail

Key facts

Isotype

IgG1

Host species

Mouse

Storage buffer

pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Form

Liquid

Clonality

Monoclonal

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
IPWBFlow Cyt (Intra)
Human
Tested
Tested
Not recommended

Tested
Tested

Species

Human

Dilution info

1/30

Notes

-

Tested
Tested

Species

Human

Dilution info

1/1000

Notes

-

Not recommended
Not recommended

Species

Human

Dilution info

-

Notes

-

Associated Products

Select an associated product type

1 product for Alternative Version

Target data

Function

Involved in the costimulatory signal, essential for T-cell proliferation and IFNG production in a PDCD1-independent manner. Interaction with PDCD1 inhibits T-cell proliferation by blocking cell cycle progression and cytokine production (By similarity).

Alternative names

Recommended products

Mouse Recombinant Monoclonal PD-L2 antibody. Suitable for IP, WB and reacts with Human samples.

Key facts

Isotype

IgG1

Form

Liquid

Clonality

Monoclonal

Clone number

CAL28

Purification technique

Affinity purification Protein A

Concentration
Loading...

Storage

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

Notes

This mouse monoclonal chimeric antibody has been engineered from a RabMAb parent antibody (Anti-PD-L2 antibody [CAL28] ab256386). By necessity, some rabbit sequence is retained as part of the variable domain. When multiplexing with other rabbit-derived antibodies, using cross absorbed Fc-reactive secondary antibodies are recommended.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.

Activity summary

PD-L2 also known as PDCD1LG2 or B7-DC is a member of the B7 protein family that plays a role in immune system regulation. This protein has a molecular mass of approximately 25 kDa. PD-L2 is expressed in antigen-presenting cells such as dendritic cells and macrophages. Expression is also located in some subsets of T cells and B cells. The PD-L2 can act as a second ligand for the PD-1 receptor which is important in immune checkpoints.

Biological function summary

PD-L2 offers protection to tissues from autoimmunity by regulating T-cell activation and fostering tolerance. By engaging with its receptor PD-1 PD-L2 forms a complex that modulates the immune response to prevent overactivation. This interaction limits the immune system's potential damage to healthy tissues maintaining proper immune function in diverse environments.

Pathways

PD-L2 participates in the immune checkpoint pathways where it works closely with PD-1 to mediate immune responses. PD-L2 forms an important part of the program death-1 (PD-1) pathway which plays a prominent role in downregulating T-cell activity. It relates to proteins such as PD-L1 which also interacts with PD-1 modulating immune tolerance and response to infections or tumors.

Associated diseases and disorders

PD-L2 involvement has been significant in cancer immunology and autoimmune conditions. Its interaction with PD-1 relates to the suppression of immune responses in tumors contributing to cancer progression. PD-L2's function as an immune checkpoint ligand associates with autoimmune diseases like rheumatoid arthritis where immune regulation goes awry. Both PD-1 and PD-L2 represent potential targets for therapeutic interventions due to their roles in these conditions.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

2 product images

  • Western blot - Anti-PD-L2 antibody [CAL28] – Mouse IgG1 (Chimeric) (ab283344), expandable thumbnail

    Western blot - Anti-PD-L2 antibody [CAL28] – Mouse IgG1 (Chimeric) (ab283344)

    Blocking and diluting buffer: 5% NFDM/TBST

    Exposure time: 26 seconds

    Negative control: Jurkat. (PMID: 27631416).

    All lanes: Western blot - Anti-PD-L2 antibody [CAL28] – Mouse IgG1 (Chimeric) (ab283344) at 1/1000 dilution

    Lane 1: HDLM-2 (human Hodgkin lymphoma) whole cell lysate at 20 µg

    Lane 2: Jurkat (human T cell leukemia cell line from peripheral blood) whole cell lysate at 20 µg

    Secondary

    All lanes: Peroxidase-Conjugated Goat anti-Mouse IgG (H+L) at 1/5000 dilution

    Predicted band size: 31 kDa

    Observed band size: 45-60 kDa

  • Immunoprecipitation - Anti-PD-L2 antibody [CAL28] – Mouse IgG1 (Chimeric) (ab283344), expandable thumbnail

    Immunoprecipitation - Anti-PD-L2 antibody [CAL28] – Mouse IgG1 (Chimeric) (ab283344)

    PD-L2 was immunoprecipitated from 0.35 mg HDLM-2 whole cell lysate 10 ug with ab283344 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab283344 at 1/1000 dilution. mouse IgG for IP (HRP) (Anti-mouse IgG for IP (HRP) ab131368) was used at 1/10000 dilution.

    Lane 1: HDLM-2 (human Hodgkin lymphoma) whole cell lysate 10 μg

    Lane 2: ab283344 IP in HDLM-2 whole cell lysate

    Lane 3: Mouse monoclonal IgG (Mouse IgG1, kappa monoclonal [MOPC-21] - isotype control ab18443) instead of Anti-PD-L2 antibody [CAL28] - Rat IgG2a (Chimeric) ab283347 in THDLM-2 whole cell lysate

    Blocking and dilution buffer and concentration: 5% NFDM/TBST.

    Exposure time: 41 seconds

    All lanes: Immunoprecipitation - Anti-PD-L2 antibody [CAL28] – Mouse IgG1 (Chimeric) (ab283344)

    Predicted band size: 31 kDa

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

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