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AB324957

Anti-PD-L2 antibody [EPR30468-530] - BSA and Azide free

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Rabbit Recombinant Monoclonal PD-L2 antibody. Carrier free. Suitable for Flow Cyt (Intra), Flow Cyt and reacts with Transfected cell line - Mouse, Mouse samples.

View Alternative Names

CD273, B7dc, Btdc, Cd273, Pdl2, Pdcd1lg2, Programmed cell death 1 ligand 2, PD-1 ligand 2, PD-L2, PDCD1 ligand 2, Programmed death ligand 2, Butyrophilin B7-DC, B7-DC

4 Images
Flow Cytometry - Anti-PD-L2 antibody [EPR30468-530] - BSA and Azide free (AB324957)
  • Flow Cyt

Supplier Data

Flow Cytometry - Anti-PD-L2 antibody [EPR30468-530] - BSA and Azide free (AB324957)

This data was developed using ab324944, the same antibody clone in a different buffer formulation.

Flow cytometric analysis of Mouse bone marrow derived Dendritic cells induced by 20ng/ml GM-CSF for 13 days and 50ng/ml IL4 for 7 days (Lower left and right) / Mouse bone marrow (Upper left and right) cells labelling PD-L2 with ab324944 at 1/500 dilution (0.1ug) / Upper right and Lower right compared with a Rabbit monoclonal IgG (ab172730) / Left isotype control.

Goat Anti-Rabbit IgG (Alexa Fluor® 488, ab150081) at 1/5000 dilution was used as the secondary antibody.

Cells were surface stained with anti-CD11c conjugated to BV421 and anti-MHCII conjugated to APC/CY7 .
Gated on viable CD11c positive cells.

Flow Cytometry - Anti-PD-L2 antibody [EPR30468-530] - BSA and Azide free (AB324957)
  • Flow Cyt

Supplier Data

Flow Cytometry - Anti-PD-L2 antibody [EPR30468-530] - BSA and Azide free (AB324957)

This data was developed using ab324944, the same antibody clone in a different buffer formulation.

Flow cytometric analysis of Mouse spleen cell treated with 5ng/ml mouse GM-CSF overnight (Lower left and right) / Untreated control (Upper left and right) cells labelling PD-L2 with ab324944 at 1/500 dilution (0.1ug) / Upper right and Lower right compared with a Rabbit monoclonal IgG (ab172730) / Left isotype control.

Goat Anti-Rabbit IgG (Alexa Fluor® 488, ab150081) at 1/5000 dilution was used as the secondary antibody.

Cells were stained with anti-CD3 conjugated to Alexa Fluor®647.
Gated on viable cells.

Flow Cytometry - Anti-PD-L2 antibody [EPR30468-530] - BSA and Azide free (AB324957)
  • Flow Cyt

Supplier Data

Flow Cytometry - Anti-PD-L2 antibody [EPR30468-530] - BSA and Azide free (AB324957)

This data was developed using ab324944, the same antibody clone in a different buffer formulation.

Flow cytometric analysis of Mouse spleen cell treated with 5ng/ml mouse GM-CSF overnight (Lower left and right) / Untreated control (Upper left and right) cells labelling PD-L2 with ab324944 at 1/500 dilution (0.1ug) / Upper right and Lower right compared with a Rabbit monoclonal IgG (ab172730) / Left isotype control.

Goat Anti-Rabbit IgG (Alexa Fluor® 488, ab150081) at 1/5000 dilution was used as the secondary antibody.

Cells were stained with anti-CD11c conjugated to Brilliant Violet 421.
Gated on viable cells.

Flow Cytometry (Intracellular) - Anti-PD-L2 antibody [EPR30468-530] - BSA and Azide free (AB324957)
  • Flow Cyt (Intra)

Supplier Data

Flow Cytometry (Intracellular) - Anti-PD-L2 antibody [EPR30468-530] - BSA and Azide free (AB324957)

This data was developed using ab324944, the same antibody clone in a different buffer formulation.

Flow cytometric analysis of 2% paraformaldehyde fixed 0.1% Tween-20 permeabilized Isotype (Left) / 293T cells transfected with a mouse PDCD1LG2 expression vector containing a myc-His-tag® (Middle) / 293T cells transfected with an empty expression vector containing a myc-His-tag® (Right) cells labelling PD-L2 with ab324944 at 1/50000 dilution (0.001ug) / Middle and Right compared with a Rabbit monoclonal IgG (ab172730) / Left isotype control.

Goat Anti-Rabbit IgG (Alexa Fluor® 488, ab150081) at 1/5000 dilution was used as the secondary antibody.

Cells were surface stained with isotype control or our antibody. Then fixed with 2% PFA for 10min followed by intracellularly stained with anti-myc tag conjugated to Alexa Fluor® 647.

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR30468-530

Isotype

IgG

Carrier free

Yes

Reacts with

Mouse

Applications

Flow Cyt, Flow Cyt (Intra)

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Mouse": { "FlowCytIntra-species-checked": "guaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "", "FlowCyt-species-checked": "testedAndGuaranteed", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "<p></p>", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>", "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>" }, "Transfected cell line - Mouse": { "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "<p></p>", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "" } } }
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Product details

ab324957 is the carrier-free version of ab324944

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 - 7.4 Constituents: 100% PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
Do Not Freeze
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Involved in the costimulatory signal essential for T-cell proliferation and IFNG production in a PDCD1-independent manner. Interaction with PDCD1 inhibits T-cell proliferation by blocking cell cycle progression and cytokine production.
See full target information Pdcd1lg2
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions
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Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com