AB92484

Anti-PD1 antibody

Name: Anti-PD1 antibody (ab92484) | Abcam
Brand: Abcam Limited
SKU: AB92484

(1 Review)

(5 Publications)

Rabbit Polyclonal PD1 antibody. Suitable for IHC-P and reacts with Human samples. Cited in 5 publications. Immunogen corresponding to Synthetic Peptide within Human PDCD1 conjugated to Keyhole Limpet Haemocyanin.

View Alternative Names

CD279, PD1, PDCD1, Programmed cell death protein 1, Protein PD-1, hPD-1

1 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PD1 antibody (AB92484)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PD1 antibody (AB92484)

ab92484, at 10 μg/ml, staining PD1 in formalin fixed, paraffin embedded Human small intestine tissue by Immunohistochemistry.

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Human

Applications

IHC-P

applications

Immunogen

Synthetic Peptide within Human PDCD1 conjugated to Keyhole Limpet Haemocyanin. The exact immunogen used to generate this antibody is proprietary information.

Q15116

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "10 µg/mL", "IHCP-species-notes": "<p></p>" }, "Mouse": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Rat": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Storage buffer

pH: 7.4 Preservative: 0.02% Sodium azide Constituents: PBS

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

PD1 also known as Programmed Cell Death Protein 1 or PDCD1 is a transmembrane protein that plays a critical role in regulating immune responses. It has a mass of approximately 55 kDa. PD1 is expressed on the surface of T cells B cells and some myeloid cells. PD1’s expression increases upon activation of these immune cells assisting in maintaining peripheral tolerance. Researchers often use PD1 mouse models and chimeric antibodies to explore the function of PD1 for experimental purposes. Antibodies such as anti-PD1 such as EH12.2H7 help in blocking PD1 interaction to study its role further.

Biological function summary

PD1 serves as an inhibitory receptor acting as a checkpoint in the immune system. It becomes part of an immune-suppressive complex when it binds with its ligands PD-L1 or PD-L2 which are expressed on various cell types including some tumor cells. This interaction suppresses the proliferation of T cells and cytokine production contributing to immune homeostasis. By controlling T cell activity PD1 limits autoimmunity but can also reduce the immune system's capability to attack cancer cells.

Pathways

PD1 functions in the immune checkpoint pathway a critical regulatory circuit in immune regulation. The engagement of PD1 with its ligands initiates a cascade that inhibits the function and proliferation of T cells through downstream SHP-2 phosphatase activity. This pathway frequently involves other regulatory proteins like CTLA-4 and is an important mechanism by which the body modulates immune responses. Related pathways often intersect with those involving T cell receptor signaling and contribute to the overall modulation of immune activity.

PD1 has a significant role in cancer and autoimmune disorders. PD1 expression can allow tumors to evade immune surveillance making PD1 a target for cancer therapies such as anti-PD1 antibodies which aim to block PD1 and restore T cell activity. The interaction of PD1 with cancer-related proteins like PD-L1 facilitates tumor immune evasion. In autoimmune disorders PD1’s regulation of immune balance can become dysregulated leading to persistent immune activation and tissue damage. Understanding PD1 and its interaction with proteins such as PD-L1 helps in developing therapeutic strategies for both cancer and autoimmune conditions.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

The protein expressed by the gene PDCD1 functions as an inhibitory receptor on antigen-activated T-cells, playing a critical role in the induction and maintenance of immune tolerance to self. It delivers inhibitory signals by binding to the ligands CD274/PDCD1L1 and CD273/PDCD1LG2. Tumors exploit the PDCD1-mediated inhibitory pathway to attenuate anti-tumor immunity, enabling tumor survival by evading immune system destruction. The interaction with CD274/PDCD1L1 inhibits the effector function of cytotoxic T lymphocytes (CTLs). Blocking the PDCD1-mediated pathway can reverse the exhausted T-cell phenotype and normalize the anti-tumor response, providing a rationale for cancer immunotherapy. This supplementary information is collated from multiple sources and compiled automatically.

See full target information PDCD1

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Publications (5)

Recent publications for all applications. Explore the full list and refine your search

Frontiers in neuroscience 16:816174 PubMed35401074

2022

Mitigating Effect of Estrogen in Alzheimer's Disease-Mimicking Cerebral Organoid.

Applications

Unspecified application

Species

Unspecified reactive species

Jennifer Yejean Kim,Hyunkyung Mo,Juryun Kim,Jang Woon Kim,Yoojun Nam,Yeri Alice Rim,Ji Hyeon Ju

Immunologic research 70:256-268 PubMed35043369

2022

Analysis of PD-1, PD-L1, and T-cell infiltration in angiosarcoma pathogenetic subgroups.

Applications

Unspecified application

Species

Unspecified reactive species

T Tomassen,M E Weidema,M H S Hillebrandt-Roeffen,C van der Horst,I M E Desar,U E Flucke,Yvonne M H Versleijen-Jonkers

Journal of personalized medicine 10: PubMed33096722

2020

Why Does COVID-19 Affect Patients with Spinal Cord Injury Milder? A Case-Control Study: Results from Two Observational Cohorts.

Applications

Unspecified application

Species

Unspecified reactive species

Enrique Calvo,Nerea Corbacho-Alonso,Tamara Sastre-Oliva,Estefania Nuñez,Patricia Baena-Galan,German Hernandez-Fernandez,Miguel Rodriguez-Cola,Irena Jimenez-Velasco,Fernando J Corrales,Claudia Gambarrutta-Malfati,Francisco Gutierrez-Henares,Elisa Lopez-Dolado,Angel Gil-Agudo,Jesus Vazquez,Laura Mourino-Alvarez,Maria G Barderas

Journal of immunotherapy (Hagerstown, Md. : 1997) 43:169-174 PubMed32224717

2020

Expression of Programmed Cell Death Proteins in Kaposi Sarcoma and Cutaneous Angiosarcoma.

Applications

Unspecified application

Species

Unspecified reactive species

Thilo Gambichler,Sonja Koim,Malwina Wrobel,Heiko U Käfferlein,Thomas Brüning,Eggert Stockfleth,Jürgen C Becker,Kerstin Lang

Journal of clinical medicine 8: PubMed31581482

2019

Correlation between B7-H4 and Survival of Non-Small-Cell Lung Cancer Patients Treated with Nivolumab.

Applications

Unspecified application

Species

Unspecified reactive species

Carlo Genova,Simona Boccardo,Marco Mora,Erika Rijavec,Federica Biello,Giovanni Rossi,Marco Tagliamento,Maria Giovanna Dal Bello,Simona Coco,Angela Alama,Irene Vanni,Giulia Barletta,Rita Bianchi,Claudia Maggioni,Paolo Bruzzi,Francesco Grossi

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