Anti-PD1 antibody [EPR20665] (ab214421) is a rabbit monoclonal antibody detecting PD1 in Western Blot, IHC-P, ICC/IF. Suitable for Mouse.
- Biophysical QC for unrivalled batch-batch consistency
- Over 30 publications
Images
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PD1 antibody [EPR20665] (AB214421), expandable thumbnail](https://content.abcam.com/products/images/pd1-antibody-epr20665-ab214421--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img104167.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PD1 antibody [EPR20665] (AB214421)")
![Immunocytochemistry/ Immunofluorescence - Anti-PD1 antibody [EPR20665] (AB214421), expandable thumbnail](https://content.abcam.com/products/images/pd1-antibody-epr20665-ab214421--immunocytochemistry-immunofluorescence-img118167.jpg/_jcr_content/renditions/webp-475.webp "Immunocytochemistry/ Immunofluorescence - Anti-PD1 antibody [EPR20665] (AB214421)")
![Western blot - Anti-PD1 antibody [EPR20665] (AB214421), expandable thumbnail](https://content.abcam.com/products/images/pd1-antibody-epr20665-ab214421--western-blot-img83387.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-PD1 antibody [EPR20665] (AB214421)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PD1 antibody [EPR20665] (AB214421), expandable thumbnail](https://content.abcam.com/products/images/pd1-antibody-epr20665-ab214421--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img104758.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PD1 antibody [EPR20665] (AB214421)")
Publications
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- International journal of molecular sciences 24:2023Evaluation of Immune Infiltrates in Ovarian Endometriosis and Endometriosis-Associated Ovarian Cancer: Relationship with Histological and Clinical Features.Applications:Unspecified applicationReactive species:Unspecified reactive speciesEmanuela Spagnolo,Alejandra Martinez,Andrea Mascarós-Martínez,Josep Marí-Alexandre,María Carbonell,Eva González-Cantó,Eva Manuela Pena-Burgos,Bárbara Andrea Mc Cormack,Sarai Tomás-Pérez,Juan Gilabert-Estellés,Ana López-Carrasco,Paula Hidalgo,Martina Aida Ángeles,Andrés Redondo,Alejandro Gallego,Alicia HernándezPubMed 37569458
- Journal of translational medicine 21:4672023Flubendazole inhibits PD-1 and suppresses melanoma growth in immunocompetent mice.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYue Li,Ben Wu,Md Jakir Hossain,Lily Quagliata,Connor O'Meara,Marc R Wilkins,Susan Corley,Levon M KhachigianPubMed 37452307
- NPJ vaccines 8:432023SARS-CoV-2 spike-ferritin-nanoparticle adjuvanted with ALFQ induces long-lived plasma cells and cross-neutralizing antibodies.Applications:Unspecified applicationReactive species:Unspecified reactive speciesShikha Shrivastava,Joshua M Carmen,Zhongyan Lu,Shraddha Basu,Rajeshwer S Sankhala,Wei-Hung Chen,Phuong Nguyen,William C Chang,Jocelyn King,Courtney Corbitt,Sandra Mayer,Jessica S Bolton,Alexander Anderson,Isabella Swafford,Guillermo D Terriquez,Hung V Trinh,Jiae Kim,Ousman Jobe,Dominic Paquin-Proulx,Gary R Matyas,Gregory D Gromowski,Jeffrey R Currier,Elke Bergmann-Leitner,Kayvon Modjarrad,Nelson L Michael,M Gordon Joyce,Allison M W Malloy,Mangala RaoPubMed 36934088
- Clinical and translational medicine 12:e11182022The dual role of CD70 in B-cell lymphomagenesis.Applications:Unspecified applicationReactive species:Unspecified reactive speciesMan Nie,Weicheng Ren,Xiaofei Ye,Mattias Berglund,Xianhuo Wang,Karin Fjordén,Likun Du,Yvonne Giannoula,Dexin Lei,Wenjia Su,Wei Li,Dongbing Liu,Johan Linderoth,Chengyi Jiang,Huijing Bao,Wenqi Jiang,Huiqiang Huang,Yong Hou,Shida Zhu,Gunilla Enblad,Mats Jerkeman,Kui Wu,Huilai Zhang,Rose-Marie Amini,Zhi-Ming Li,Qiang Pan-HammarströmPubMed 36471481
- Breast cancer (Dove Medical Press) 14:391-4032022Metformin Inducing the Change of Functional and Exhausted Phenotypic Tumor-Infiltrated Lymphocytes and the Correlation with JNK Signal Pathway in Triple-Negative Breast Cancer.Applications:Unspecified applicationReactive species:Unspecified reactive speciesRuibin Wang,Yuchen Li,Yanjie Zhao,Feng Shi,Quan Zhou,Jiangping Wu,Shuzhen Lyu,Qingkun SongPubMed 36482884
- Ophthalmic research 65:584-5942022Dynamic Observation: Immune-Privileged Microenvironment Limited the Effectiveness of Immunotherapy in an Intraocular Metastasis Mouse Model.Applications:Unspecified applicationReactive species:Unspecified reactive speciesTianchang Tao,Yang Liu,Jun Zhang,Lvzhen Huang,Ye TaoPubMed 35398850
- Journal of experimental & clinical cancer research : CR 41:1322022Adaptive antitumor immune response stimulated by bio-nanoparticle based vaccine and checkpoint blockade.Applications:Unspecified applicationReactive species:Unspecified reactive speciesXuewei Bai,Yanmei Zhou,Yuki Yokota,Yoshihiro Matsumoto,Bo Zhai,Nader Maarouf,Hikaru Hayashi,Rolf Carlson,Songhua Zhang,Aryanna Sousa,Bei Sun,Hossein Ghanbari,Xiaoqun Dong,Jack R WandsPubMed 35392977
- Frontiers in immunology 13:8379912022A Metabolism-Related Gene Landscape Predicts Prostate Cancer Recurrence and Treatment Response.Applications:Unspecified applicationReactive species:Unspecified reactive speciesLijie Zhou,Ruixin Fan,Yongbo Luo,Cai Zhang,Donghui Jia,Rongli Wang,Youmiao Zeng,Mengda Ren,Kaixuan Du,Wenbang Pan,Jinjian Yang,Fengyan Tian,Chaohui GuPubMed 35359973
- Nature cancer 3:337-3542022An anti-PD-1-GITR-L bispecific agonist induces GITR clustering-mediated T cell activation for cancer immunotherapy.Applications:Unspecified applicationReactive species:Unspecified reactive speciesSarah Chan,Nicole Belmar,Sun Ho,Bryan Rogers,Marcia Stickler,Michelle Graham,Eileen Lee,Ninian Tran,Dong Zhang,Priyanka Gupta,Mien Sho,Tracy MacDonough,Andrew Woolley,Han Kim,Hong Zhang,Wei Liu,Pingping Zheng,Zoltan Dezso,Kyle Halliwill,Michele Ceccarelli,Susan Rhodes,Archana Thakur,Charles M Forsyth,Mengli Xiong,Siu Sze Tan,Ramesh Iyer,Marc Lake,Enrico Digiammarino,Li Zhou,Lance Bigelow,Kenton Longenecker,Russell A Judge,Cassie Liu,Max Trumble,Jonathan P Remis,Melvin Fox,Belinda Cairns,Yoshiko Akamatsu,Diane Hollenbaugh,Fiona Harding,Hamsell M AlvarezPubMed 35256819
- Molecular imaging 2022:59166922022Zr Immuno-PET Imaging of Tumor PD-1 Reveals That PMA Upregulates Lymphoma PD-1 through NFB and JNK Signaling.Applications:Unspecified applicationReactive species:Unspecified reactive speciesKyung-Ho Jung,Jin Hee Lee,Mina Kim,Young Seok Cho,Kyung-Han LeePubMed 35250391
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Abcam Recommends
Reactivity data
Select an application| WB | ICC/IF | IHC-Fr | IHC-P | |
|---|---|---|---|---|
| Mouse | Tested | Tested | Not recommended | Tested |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info 1/1000 | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info 1/100 | Notes - |
IHC-Fr
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info - | Notes - |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info 1/1000 | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Associated Products
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15 products for Alternative Product
Target data
Function
Inhibitory receptor on antigen activated T-cells that plays a critical role in induction and maintenance of immune tolerance to self (PubMed:10485649, PubMed:11209085, PubMed:11698646, PubMed:21300912). Delivers inhibitory signals upon binding to ligands, such as CD274/PDCD1L1 and CD273/PDCD1LG2 (PubMed:11015443, PubMed:11224527, PubMed:18287011, PubMed:18641123, PubMed:22641383). Following T-cell receptor (TCR) engagement, PDCD1 associates with CD3-TCR in the immunological synapse and directly inhibits T-cell activation (PubMed:22641383). Suppresses T-cell activation through the recruitment of PTPN11/SHP-2: following ligand-binding, PDCD1 is phosphorylated within the ITSM motif, leading to the recruitment of the protein tyrosine phosphatase PTPN11/SHP-2 that mediates dephosphorylation of key TCR proximal signaling molecules, such as ZAP70, PRKCQ/PKCtheta and CD247/CD3zeta (PubMed:11698646, PubMed:22641383). The PDCD1-mediated inhibitory pathway is exploited by tumors to attenuate anti-tumor immunity and facilitate tumor survival (By similarity).
Alternative names
CD279, Pd1, Pdcd1, Programmed cell death protein 1, Protein PD-1, mPD-1
Recommended products
Anti-PD1 antibody [EPR20665] (ab214421) is a rabbit monoclonal antibody detecting PD1 in Western Blot, IHC-P, ICC/IF. Suitable for Mouse.
- Biophysical QC for unrivalled batch-batch consistency
- Over 30 publications
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA- Form
- Liquid
- Clonality
- Monoclonal
- Immunogens
- The exact immunogen used to generate this antibody is proprietary information.
- Clone number
- EPR20665
- Purification technique
- Affinity purification Protein A
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
Anti-PD1 antibody [EPR20665] (ab214421) was developed by Abcam using patented rabbit monoclonal antibody technology and is validated for use in ICC/IF, IHC-P, WB in mouse samples.
Anti-PD1 antibody [EPR20665] (ab214421) specifically detects PD1 (UniProt ID: Q02242; Molecular weight: 29kDa) and is sold in a convenient trial size to enable initial testing (20 µL) and larger sizes for subsequent scaling up experiments (100 µL and 1 mL).
Quality and Validation
Abcam's high quality manufacturing and validation processes ensure Anti-PD1 antibody [EPR20665] (ab214421) has high sensitivity and specificity alongside high lot-to-lot consistency and reproducibility.
Anti-PD1 antibody [EPR20665] (ab214421) has been cited over 33 times in peer reviewed journals and is trusted by the scientific community.
Related Products
Antibody clone EPR20665 is also available pre-conjugated to a variety of labels for your convenience - Alexa Fluor® 488, Alexa Fluor® 647, Alexa Fluor® 594, Alexa Fluor® 568, Alexa Fluor® 555, Alexa Fluor® 750 (Alexa Fluor® 488 Anti-PD1 antibody [EPR20665] ab311004, Alexa Fluor® 647 Anti-PD1 antibody [EPR20665] ab311132, Alexa Fluor® 594 Anti-PD1 antibody [EPR20665] ab311765, Alexa Fluor® 568 Anti-PD1 antibody [EPR20665] ab313046, Alexa Fluor® 555 Anti-PD1 antibody [EPR20665] ab313247, Alexa Fluor® 750 Anti-PD1 antibody [EPR20665] ab321377).
Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
4 product images
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PD1 antibody [EPR20665] (ab214421), expandable thumbnail](https://content.abcam.com/products/images/pd1-antibody-epr20665-ab214421--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img104167.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PD1 antibody [EPR20665] (ab214421)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PD1 antibody [EPR20665] (ab214421)
Immunohistochemical analysis of paraffin-embedded mouse spleen tissue labeling PD1 with ab214421 at 1/1000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Cytoplasmic staining on the germinal center of mouse spleen. (PMID: 21750671, PMID: 17640856). Counter stained with hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use.
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
![Immunocytochemistry/ Immunofluorescence - Anti-PD1 antibody [EPR20665] (ab214421), expandable thumbnail](https://content.abcam.com/products/images/pd1-antibody-epr20665-ab214421--immunocytochemistry-immunofluorescence-img118167.jpg "Immunocytochemistry/ Immunofluorescence - Anti-PD1 antibody [EPR20665] (ab214421)")
Immunocytochemistry/ Immunofluorescence - Anti-PD1 antibody [EPR20665] (ab214421)
Immunofluorescent analysis of 100% methanol-fixed EL4.IL-2 (mouse lymphoma T lymphocyte) cells labeling PD1 with ab214421 at 1/100 dilution, followed by Goat anti-rabbit IgG (Alexa Fluor® 488) (Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) secondary antibody at 1/1000 dilution (green). Confocal image showing cytoplasmic staining on EL4.IL-2 cell line.
The nuclear counterstain is DAPI (blue). Tubulin is detected with Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) (Alexa Fluor® 594 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker ab195889) (red) at 1/200 dilution.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) secondary antibody at 1/1000 dilution.
![Western blot - Anti-PD1 antibody [EPR20665] (ab214421), expandable thumbnail](https://content.abcam.com/products/images/pd1-antibody-epr20665-ab214421--western-blot-img83387.jpg "Western blot - Anti-PD1 antibody [EPR20665] (ab214421)")
Western blot - Anti-PD1 antibody [EPR20665] (ab214421)
Blocking/Dilution buffer: 5% NFDM/TBST.All lanes: Western blot - Anti-PD1 antibody [EPR20665] (ab214421) at 1/1000 dilution
All lanes: EL4.IL-2 (mouse lymphoma T lymphocyte) whole cell lysate at 10 µg
SecondaryAll lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution
Developed using the ECL technique.
Predicted band size: 32 kDa
Observed band size: 50-55 kDa
Exposure time: 3min
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PD1 antibody [EPR20665] (ab214421), expandable thumbnail](https://content.abcam.com/products/images/pd1-antibody-epr20665-ab214421--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img104758.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PD1 antibody [EPR20665] (ab214421)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PD1 antibody [EPR20665] (ab214421)
Immunohistochemical analysis of paraffin-embedded mouse thymus tissue labeling PD1 with ab214421 at 1/1000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Sporadic cytoplasmic staining on mouse thymus medulla. (PMID: 21750671, PMID: 17640856). Counter stained with hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use.
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
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