AB214421

Anti-PD1 antibody [EPR20665]

Name: Anti-PD1 antibody [EPR20665]. Rabbit Monoclonal (ab214421) | Abcam
Brand: Abcam Limited
SKU: AB214421
Price: 215 USD
Availability: InStock
Rating: 4 (3 reviews)

20ul selling size
RabMAb
Recombinant
What is this?

(3 Reviews)

(80 Publications)

Anti-PD1 antibody [EPR20665] (ab214421) is a rabbit monoclonal antibody detecting PD1 in Western Blot, IHC-P, ICC/IF. Suitable for Mouse.

- Biophysical QC for unrivalled batch-batch consistency
- Over 30 publications

View Alternative Names

CD279, Pd1, Pdcd1, Programmed cell death protein 1, Protein PD-1, mPD-1

4 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PD1 antibody [EPR20665] (AB214421)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PD1 antibody [EPR20665] (AB214421)

Immunohistochemical analysis of paraffin-embedded mouse spleen tissue labeling PD1 with ab214421 at 1/1000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Cytoplasmic staining on the germinal center of mouse spleen. (PMID : 21750671, PMID : 17640856). Counter stained with hematoxylin.

Secondary antibody only control : Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use.

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Immunocytochemistry/ Immunofluorescence - Anti-PD1 antibody [EPR20665] (AB214421)

ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-PD1 antibody [EPR20665] (AB214421)

Immunofluorescent analysis of 100% methanol-fixed EL4.IL-2 (mouse lymphoma T lymphocyte) cells labeling PD1 with ab214421 at 1/100 dilution, followed by Goat anti-rabbit IgG (Alexa Fluor^® 488) (ab150077) secondary antibody at 1/1000 dilution (green). Confocal image showing cytoplasmic staining on EL4.IL-2 cell line.

The nuclear counterstain is DAPI (blue). Tubulin is detected with Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor^® 594) (ab195889) (red) at 1/200 dilution.

Secondary antibody only control : Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 488) (ab150077) secondary antibody at 1/1000 dilution.

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PD1 antibody [EPR20665] (AB214421)

Immunohistochemical analysis of paraffin-embedded mouse thymus tissue labeling PD1 with ab214421 at 1/1000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Sporadic cytoplasmic staining on mouse thymus medulla. (PMID : 21750671, PMID : 17640856). Counter stained with hematoxylin.

Secondary antibody only control : Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use.

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Supplier Data

Western blot - Anti-PD1 antibody [EPR20665] (AB214421)

Blocking/Dilution buffer : 5% NFDM/TBST.

All lanes:

Western blot - Anti-PD1 antibody [EPR20665] (ab214421) at 1/1000 dilution

All lanes:

EL4.IL-2 (mouse lymphoma T lymphocyte) whole cell lysate at 10 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Predicted band size: 32 kDa

Observed band size: 50-55 kDa

true

Exposure time: 3min

Carrier free

Anti-PD1 antibody [EPR20665] - BSA and Azide free
603 Alexa Fluor® 568

Alexa Fluor® 568 Anti-PD1 antibody [EPR20665]
565 Alexa Fluor® 555

Alexa Fluor® 555 Anti-PD1 antibody [EPR20665]
617 Alexa Fluor® 594

Alexa Fluor® 594 Anti-PD1 antibody [EPR20665]
519 Alexa Fluor® 488

Alexa Fluor® 488 Anti-PD1 antibody [EPR20665]
665 Alexa Fluor® 647

Alexa Fluor® 647 Anti-PD1 antibody [EPR20665]
775 Alexa Fluor® 750

Alexa Fluor® 750 Anti-PD1 antibody [EPR20665]

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR20665

Isotype

IgG

Carrier free

Reacts with

Mouse

Applications

IHC-P, ICC/IF, WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "IHCFr" : {"fullname" : "Immunohistochemistry (Frozen sections)", "shortname":"IHC-Fr"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/100", "ICCIF-species-notes": "", "IHCFr-species-checked": "notRecommended", "IHCFr-species-dilution-info": "", "IHCFr-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/1000", "IHCP-species-notes": " Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol." } } }

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Product details

Product Specifications
Anti-PD1 antibody [EPR20665] (ab214421) was developed by Abcam using patented rabbit monoclonal antibody technology and is validated for use in ICC/IF, IHC-P, WB in mouse samples.
Anti-PD1 antibody [EPR20665] (ab214421) specifically detects PD1 (UniProt ID: Q02242; Molecular weight: 29kDa) and is sold in a convenient trial size to enable initial testing (20 µL) and larger sizes for subsequent scaling up experiments (100 µL and 1 mL).

Quality and Validation
Abcam's high quality manufacturing and validation processes ensure Anti-PD1 antibody [EPR20665] (ab214421) has high sensitivity and specificity alongside high lot-to-lot consistency and reproducibility.
Anti-PD1 antibody [EPR20665] (ab214421) has been cited over 33 times in peer reviewed journals and is trusted by the scientific community.

Related Products
Antibody clone EPR20665 is also available pre-conjugated to a variety of labels for your convenience - Alexa Fluor® 488, Alexa Fluor® 647, Alexa Fluor® 594, Alexa Fluor® 568, Alexa Fluor® 555, Alexa Fluor® 750 (ab311004, ab311132, ab311765, ab313046, ab313247, ab321377).

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

PD1 also known as Programmed Cell Death Protein 1 or PDCD1 is a transmembrane protein that plays a critical role in regulating immune responses. It has a mass of approximately 55 kDa. PD1 is expressed on the surface of T cells B cells and some myeloid cells. PD1’s expression increases upon activation of these immune cells assisting in maintaining peripheral tolerance. Researchers often use PD1 mouse models and chimeric antibodies to explore the function of PD1 for experimental purposes. Antibodies such as anti-PD1 such as EH12.2H7 help in blocking PD1 interaction to study its role further.

Biological function summary

PD1 serves as an inhibitory receptor acting as a checkpoint in the immune system. It becomes part of an immune-suppressive complex when it binds with its ligands PD-L1 or PD-L2 which are expressed on various cell types including some tumor cells. This interaction suppresses the proliferation of T cells and cytokine production contributing to immune homeostasis. By controlling T cell activity PD1 limits autoimmunity but can also reduce the immune system's capability to attack cancer cells.

Pathways

PD1 functions in the immune checkpoint pathway a critical regulatory circuit in immune regulation. The engagement of PD1 with its ligands initiates a cascade that inhibits the function and proliferation of T cells through downstream SHP-2 phosphatase activity. This pathway frequently involves other regulatory proteins like CTLA-4 and is an important mechanism by which the body modulates immune responses. Related pathways often intersect with those involving T cell receptor signaling and contribute to the overall modulation of immune activity.

PD1 has a significant role in cancer and autoimmune disorders. PD1 expression can allow tumors to evade immune surveillance making PD1 a target for cancer therapies such as anti-PD1 antibodies which aim to block PD1 and restore T cell activity. The interaction of PD1 with cancer-related proteins like PD-L1 facilitates tumor immune evasion. In autoimmune disorders PD1’s regulation of immune balance can become dysregulated leading to persistent immune activation and tissue damage. Understanding PD1 and its interaction with proteins such as PD-L1 helps in developing therapeutic strategies for both cancer and autoimmune conditions.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Inhibitory receptor on antigen activated T-cells that plays a critical role in induction and maintenance of immune tolerance to self (PubMed : 10485649, PubMed : 11209085, PubMed : 11698646, PubMed : 21300912). Delivers inhibitory signals upon binding to ligands, such as CD274/PDCD1L1 and CD273/PDCD1LG2 (PubMed : 11015443, PubMed : 11224527, PubMed : 18287011, PubMed : 18641123, PubMed : 22641383). Following T-cell receptor (TCR) engagement, PDCD1 associates with TCR-CD3 in the immunological synapse and directly inhibits T-cell activation (PubMed : 22641383). Suppresses T-cell activation through the recruitment of PTPN11/SHP-2 : following ligand-binding, PDCD1 is phosphorylated within the ITSM motif, leading to the recruitment of the protein tyrosine phosphatase PTPN11/SHP-2 that mediates dephosphorylation of key TCR proximal signaling molecules, such as ZAP70, PRKCQ/PKCtheta and CD247/CD3zeta (PubMed : 11698646, PubMed : 22641383). The PDCD1-mediated inhibitory pathway is exploited by tumors to attenuate anti-tumor immunity and facilitate tumor survival (By similarity).

See full target information Pdcd1

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Publications (80)

Recent publications for all applications. Explore the full list and refine your search

Cancer immunology, immunotherapy : CII 74:316 PubMed41021019

2025

Stress induces corticosterone-mediated CD8 T cell exhaustion to promote non-small cell lung cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Qiaoqi Zheng,Ji Zhuoga,Qian Weng,Huijie Zhang,Lingling Deng,Maimaititusun Yalikun,Peng Fu,Zaiquan Dong,Jingcheng Dong

BMC cancer 25:1417 PubMed40898094

2025

KRAS mutation promotes immune escape of lung adenocarcinoma via ZNF24/SLC7A5/PD-L1 axis.

Applications

Unspecified application

Species

Unspecified reactive species

Leilei Li,Qiang Feng,Ya Jiang,Lilin Yang,Hong Fang,Wenmang Xu,Yuanyuan Wang,Xinyan Pan,Julun Yang

Neoplasia (New York, N.Y.) 69:101222 PubMed40882404

2025

Steatohepatitis alters lymphocytes cytotoxicity and localization, accelerating colorectal liver metastases.

Applications

Unspecified application

Species

Unspecified reactive species

Adi S Yehezkel,Eyal Yehezkel,Nathalie Abudi,Rinat Abramovitch

iScience 28:112992 PubMed40687785

2025

Differential substrate degradation by super-degron: EGFP in wild-type mouse cells, PD-1 requires CRBN humanization.

Applications

Unspecified application

Species

Unspecified reactive species

Chie Naruse,Ojiro Ishibashi,Masatoshi Ohgushi,Hirohiko Imai,Tomoko Matsuzaki,Xuchi Pan,Tatsuhiko Miyazaki,Yuka Shidahara,Yu Shirakawa,Fumihiro Sugiyama,Masahide Asano

Journal for immunotherapy of cancer 13: PubMed40592739

2025

Enhanced antitumor immunity of VNP20009-CCL2-CXCL9 via the cGAS/STING axis in osteosarcoma lung metastasis.

Applications

Unspecified application

Species

Unspecified reactive species

Ruixuan Liu,Qi Liu,Yuming Wang,Tianyi Liu,Zhusheng Zhang,Chong Zhao,Haipeng Tao,Elizabeth Ogando-Rivas,Paul Castillo,Weibin Zhang

Molecular cancer 24:157 PubMed40442751

2025

Targeting BATF2-RGS2 axis reduces T-cell exhaustion and restores anti-tumor immunity.

Applications

Unspecified application

Species

Unspecified reactive species

Xuyu Gu,Chanchan Gao,Xiangyu Su,Yaoyao Zhu,Qiyu Fang,Jia Yu,Ziming Wang,Deping Zhao,Wentian Zhang

Theranostics 15:6022-6043 PubMed40365283

2025

pH-responsive nano-vaccine combined with anti-PD-1 antibodies for enhanced immunotherapy of breast cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Ning Wang,Hong Yu,Jianqiao Yin,Xiaopeng Yu

BMC cancer 25:813 PubMed40307786

2025

Single-cell sequencing unveils the transcriptomic landscape of castration-resistant prostate cancer-associated fibroblasts and their association with prognosis and immunotherapy response.

Applications

Unspecified application

Species

Unspecified reactive species

Yifeng Qiu,Yuhan Wang,Jiahe Liu,Kai Sun,Baohua Liu,Qi Hou

NPJ vaccines 10:80 PubMed40258806

2025

Targeting B7-H3 enhances the efficacy of neoantigen-based cancer vaccine in combination with radiotherapy.

Applications

Unspecified application

Species

Unspecified reactive species

Tao-Wei Ke,Chia-Yi Chen,William Tzu-Liang Chen,Yuan-Yao Tsai,Shu-Fen Chiang,Chi-Hsien Huang,Yu-Sen Lin,Te-Hong Chen,Tsung-Wei Chen,Ji-An Liang,K S Clifford Chao,Kevin Chih-Yang Huang

Theranostics 15:4481-4494 PubMed40225584

2025

Two-plex molecular imaging in the second near-infrared window for immunotherapeutic response.

Applications

Unspecified application

Species

Unspecified reactive species

Yupeng Sun,Rui Li,Yike Cai,Yan Liu,Peiyuan Wang,Ming Wu,Xiaolong Zhang,Naishun Liao,Cuilin Zhang,Aixian Zheng,Haipo Xu,Rui Zeng,Yongyi Zeng,Xiaolong Liu

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-PD1 antibody [EPR20665]

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PD1 antibody [EPR20665] (AB214421)

Immunocytochemistry/ Immunofluorescence - Anti-PD1 antibody [EPR20665] (AB214421)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PD1 antibody [EPR20665] (AB214421)

Western blot - Anti-PD1 antibody [EPR20665] (AB214421)

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Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

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Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (80)

Product promise