Rabbit Recombinant Monoclonal PD1 antibody. Carrier free. Suitable for WB, IHC-P, mIHC and reacts with Human samples.
Images
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PD1 antibody [EPR23119-111] - BSA and Azide free (AB270753), expandable thumbnail](https://content.abcam.com/products/images/pd1-antibody-epr23119-111-bsa-and-azide-free-ab270753--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img112131.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PD1 antibody [EPR23119-111] - BSA and Azide free (AB270753)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PD1 antibody [EPR23119-111] - BSA and Azide free (AB270753), expandable thumbnail](https://content.abcam.com/products/images/pd1-antibody-epr23119-111-bsa-and-azide-free-ab270753--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img23654.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PD1 antibody [EPR23119-111] - BSA and Azide free (AB270753)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PD1 antibody [EPR23119-111] - BSA and Azide free (AB270753), expandable thumbnail](https://content.abcam.com/products/images/pd1-antibody-epr23119-111-bsa-and-azide-free-ab270753--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img10984.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PD1 antibody [EPR23119-111] - BSA and Azide free (AB270753)")
![Multiplex immunohistochemistry - Anti-PD1 antibody [EPR23119-111] - BSA and Azide free (AB270753), expandable thumbnail](https://content.abcam.com/products/images/pd1-antibody-epr23119-111-bsa-and-azide-free-ab270753--multiplex-immunohistochemistry-img408702.png/_jcr_content/renditions/webp-475.webp "Multiplex immunohistochemistry - Anti-PD1 antibody [EPR23119-111] - BSA and Azide free (AB270753)")
![Multiplex immunohistochemistry - Anti-PD1 antibody [EPR23119-111] - BSA and Azide free (AB270753), expandable thumbnail](https://content.abcam.com/products/images/pd1-antibody-epr23119-111-bsa-and-azide-free-ab270753--multiplex-immunohistochemistry-img408563.png/_jcr_content/renditions/webp-475.webp "Multiplex immunohistochemistry - Anti-PD1 antibody [EPR23119-111] - BSA and Azide free (AB270753)")
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: PBS- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| ICC/IF | WB | IHC-P | mIHC | |
|---|---|---|---|---|
| Human | Not recommended | Tested | Tested | Tested |
| Mouse | Not recommended | Not recommended | Not recommended | Not recommended |
| Rat | Not recommended | Not recommended | Not recommended | Not recommended |
ICC/IF
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human, Mouse, Rat | Dilution info - | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info - | Notes - |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info - | Notes - |
mIHC
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info - | Notes - |
Associated Products
Select an associated product type
6 products for Alternative Product
Target data
Function
The protein expressed by the gene PDCD1 functions as an inhibitory receptor on antigen-activated T-cells, playing a critical role in the induction and maintenance of immune tolerance to self. It delivers inhibitory signals by binding to the ligands CD274/PDCD1L1 and CD273/PDCD1LG2. Tumors exploit the PDCD1-mediated inhibitory pathway to attenuate anti-tumor immunity, enabling tumor survival by evading immune system destruction. The interaction with CD274/PDCD1L1 inhibits the effector function of cytotoxic T lymphocytes (CTLs). Blocking the PDCD1-mediated pathway can reverse the exhausted T-cell phenotype and normalize the anti-tumor response, providing a rationale for cancer immunotherapy. This supplementary information is collated from multiple sources and compiled automatically.
Alternative names
CD279, PD1, PDCD1, Programmed cell death protein 1, Protein PD-1, hPD-1
Recommended products
Rabbit Recombinant Monoclonal PD1 antibody. Carrier free. Suitable for WB, IHC-P, mIHC and reacts with Human samples.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: PBS- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Carrier free
- Yes
- Clone number
- EPR23119-111
- Purification technique
- Affinity purification Protein A
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- +4°C
- Storage information
- Do Not Freeze
Notes
ab270753 is the carrier-free version of Anti-PD1 antibody [EPR23119-111] ab243644.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
PD1 also known as Programmed Cell Death Protein 1 or PDCD1 is a transmembrane protein that plays a critical role in regulating immune responses. It has a mass of approximately 55 kDa. PD1 is expressed on the surface of T cells B cells and some myeloid cells. PD1’s expression increases upon activation of these immune cells assisting in maintaining peripheral tolerance. Researchers often use PD1 mouse models and chimeric antibodies to explore the function of PD1 for experimental purposes. Antibodies such as anti-PD1 such as EH12.2H7 help in blocking PD1 interaction to study its role further.
- Biological function summary
PD1 serves as an inhibitory receptor acting as a checkpoint in the immune system. It becomes part of an immune-suppressive complex when it binds with its ligands PD-L1 or PD-L2 which are expressed on various cell types including some tumor cells. This interaction suppresses the proliferation of T cells and cytokine production contributing to immune homeostasis. By controlling T cell activity PD1 limits autoimmunity but can also reduce the immune system's capability to attack cancer cells.
- Pathways
PD1 functions in the immune checkpoint pathway a critical regulatory circuit in immune regulation. The engagement of PD1 with its ligands initiates a cascade that inhibits the function and proliferation of T cells through downstream SHP-2 phosphatase activity. This pathway frequently involves other regulatory proteins like CTLA-4 and is an important mechanism by which the body modulates immune responses. Related pathways often intersect with those involving T cell receptor signaling and contribute to the overall modulation of immune activity.
- Associated diseases and disorders
PD1 has a significant role in cancer and autoimmune disorders. PD1 expression can allow tumors to evade immune surveillance making PD1 a target for cancer therapies such as anti-PD1 antibodies which aim to block PD1 and restore T cell activity. The interaction of PD1 with cancer-related proteins like PD-L1 facilitates tumor immune evasion. In autoimmune disorders PD1’s regulation of immune balance can become dysregulated leading to persistent immune activation and tissue damage. Understanding PD1 and its interaction with proteins such as PD-L1 helps in developing therapeutic strategies for both cancer and autoimmune conditions.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
5 product images
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PD1 antibody [EPR23119-111] - BSA and Azide free (ab270753), expandable thumbnail](https://content.abcam.com/products/images/pd1-antibody-epr23119-111-bsa-and-azide-free-ab270753--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img112131.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PD1 antibody [EPR23119-111] - BSA and Azide free (ab270753)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PD1 antibody [EPR23119-111] - BSA and Azide free (ab270753)
Immunohistochemical analysis of paraffin-embedded Human endometrial carcinoma tissue labeling PD1 with Anti-PD1 antibody [EPR23119-111] ab243644 at 1/500 followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101). Positive staining on human endometrial carcinoma stroma (PMID: 27446374) is observed.
The section was incubated with Anti-PD1 antibody [EPR23119-111] ab243644 for 30 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101).
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-PD1 antibody [EPR23119-111] ab243644).
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PD1 antibody [EPR23119-111] - BSA and Azide free (ab270753), expandable thumbnail](https://content.abcam.com/products/images/pd1-antibody-epr23119-111-bsa-and-azide-free-ab270753--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img23654.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PD1 antibody [EPR23119-111] - BSA and Azide free (ab270753)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PD1 antibody [EPR23119-111] - BSA and Azide free (ab270753)
Immunohistochemical analysis of paraffin-embedded Human Hodgkin's lymphoma tissue labeling PD1 with Anti-PD1 antibody [EPR23119-111] ab243644 at 1/500 followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101). Positive staining on human Hodgkin's lymphoma (PMID: 16819321) is observed.
The section was incubated with Anti-PD1 antibody [EPR23119-111] ab243644 for 30 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101).
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-PD1 antibody [EPR23119-111] ab243644).
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PD1 antibody [EPR23119-111] - BSA and Azide free (ab270753), expandable thumbnail](https://content.abcam.com/products/images/pd1-antibody-epr23119-111-bsa-and-azide-free-ab270753--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img10984.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PD1 antibody [EPR23119-111] - BSA and Azide free (ab270753)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PD1 antibody [EPR23119-111] - BSA and Azide free (ab270753)
Immunohistochemical analysis of paraffin-embedded Human tonsil tissue labeling PD1 with Anti-PD1 antibody [EPR23119-111] ab243644 at 1/500 followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101). Membranous staining on germinal center of human tonsil (PMID: 29042640, PMID: 16819321) is observed.
The section was incubated with Anti-PD1 antibody [EPR23119-111] ab243644 for 30 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101).
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-PD1 antibody [EPR23119-111] ab243644).
![Multiplex immunohistochemistry - Anti-PD1 antibody [EPR23119-111] - BSA and Azide free (ab270753), expandable thumbnail](https://content.abcam.com/products/images/pd1-antibody-epr23119-111-bsa-and-azide-free-ab270753--multiplex-immunohistochemistry-img408702.png "Multiplex immunohistochemistry - Anti-PD1 antibody [EPR23119-111] - BSA and Azide free (ab270753)")
Multiplex immunohistochemistry - Anti-PD1 antibody [EPR23119-111] - BSA and Azide free (ab270753)
This data was developed using Anti-PD1 antibody [EPR23119-111] ab243644, the same antibody clone in a different buffer formulation.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human tonsil labelling PD1 with Anti-PD1 antibody [EPR23119-111] ab243644 at 1/500 dilution (1.02 µg/mL) (D), Ki67 with Anti-Ki67 antibody [SP6] ab16667 at 1/200 dilution (0.15 μg/ml) (B) and PD-L1 with Anti-PD-L1 antibody [SP142] - C-terminal ab228462 at 1/100 dilution (0.52 μg/ml) (C). Opal Polymer HRP Ms + Rb was used as a secondary antibody, and DAPI was used for a nuclear counter stain. Heat mediated antigen retrieval with Citrate buffer (pH 6.0, epitope retrieval solution 1) for 20 mins.
Panel A: merged staining of anti-Ki67 (magenta; Opal™690), anti-PD-L1 (red; Opal™570) and anti-PD1 (green; Opal™520) on human tonsil.
Panel B: anti-Ki67 stained on nucleus of proliferating cells.
Panel C: anti-PD-L1 stained on membrane of cells involved in T cell inhibition.
Panel D: anti-PD1 stained on antigen-stimulated T cells.
The section was incubated in three rounds of staining: in the order of Anti-Ki67 antibody [SP6] ab16667 for 10 mins, Anti-PD1 antibody [EPR23119-111] ab243644 for 30 mins and Anti-PD-L1 antibody [SP142] - C-terminal ab228462 for 10 mins at room temperature. Each round was followed by a separate fluorescent tyramide signal amplification system.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument with an Opal™ 4-color kit. Image acquisition was performed with Leica SP8 confocal microscope.![Multiplex immunohistochemistry - Anti-PD1 antibody [EPR23119-111] - BSA and Azide free (ab270753), expandable thumbnail](https://content.abcam.com/products/images/pd1-antibody-epr23119-111-bsa-and-azide-free-ab270753--multiplex-immunohistochemistry-img408563.png "Multiplex immunohistochemistry - Anti-PD1 antibody [EPR23119-111] - BSA and Azide free (ab270753)")
Multiplex immunohistochemistry - Anti-PD1 antibody [EPR23119-111] - BSA and Azide free (ab270753)
This data was developed using Anti-PD1 antibody [EPR23119-111] ab243644, the same antibody clone in a different buffer formulation.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human tonsil labelling PD1 with Anti-PD1 antibody [EPR23119-111] ab243644 at 1/500 dilution (1.02 µg/mL) (D), CD8 with Anti-CD8 alpha antibody [SP239] ab178089 at 1/100 dilution (0.83 μg/ml) (C) and TIM 3 with Anti-TIM 3 antibody [EPR22241] - BSA and Azide free ab242080 at 1/500 dilution (2.09 μg/ml) (B). Opal Polymer HRP Ms + Rb was used as a secondary antibody, and DAPI was used for a nuclear counter stain. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins.
Panel A: merged staining of anti-TIM 3 (green; Opal™690), anti-CD8 (red; Opal™520) and anti-PD1 (gray; Opal™570) on human tonsil.
Panel B: anti-TIM 3 stained on membrane of a subset of immune cells.
Panel C: anti-CD8 stained on membrane of a subset of T cells.
Panel D: anti-PD1 stained on membrane of a subset of lymphocytes.
The section was incubated in three rounds of staining: in the order of Anti-TIM 3 antibody [EPR22241] - BSA and Azide free ab242080, Anti-PD1 antibody [EPR23119-111] ab243644 and Anti-CD8 alpha antibody [SP239] ab178089 for 30 mins at room temperature. Each round was followed by a separate fluorescent tyramide signal amplification system.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument with an Opal™ 4-color kit. Image acquisition was performed with Leica SP8 confocal microscope.
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