Anti-PD1 antibody [EPR4877(2)] - BSA and Azide free
- BOND RX™ Validated
- RabMAb
- Recombinant
- What is this?
1
(1 Review)
|
(10 Publications)
Anti-PD1 antibody [EPR4877(2)] - BSA and Azide free (ab186928) is a rabbit recombinant monoclonal antibody provided in a PBS only buffer for easy conjugation detecting PD1 in IHC-P. Suitable for Human.
- BSA, sodium azide, and glycerol-free for easy conjugation
- Biophysical QC for unrivalled batch-batch consistency
View Alternative Names
CD279, PD1, PDCD1, Programmed cell death protein 1, Protein PD-1, hPD-1
- IHC-P
Lab
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PD1 antibody [EPR4877(2)] - BSA and Azide free (AB186928)
Immunohistochemistry (Paraffin-embedded sections) analysis of Human angioimmunoblastic T-cell lymphoma tissue labeling PD1 with ab186928 at 1/1000. Heat mediated antigen retrieval was perfomed using Tris/EDTA buffer pH 9. Anti-rabbit IgG H&L (HRP) (ab97051) was used as the secondary antibody at 1/500. Counterstained with hematoxylin.
- IHC-P
Unknown
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PD1 antibody [EPR4877(2)] - BSA and Azide free (AB186928)
Immunohistochemical analysis using ab137132 showing negative staining in human skeletal muscle tissue.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab137132).
- IHC-P
Unknown
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PD1 antibody [EPR4877(2)] - BSA and Azide free (AB186928)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human tonsil tissue labelling PD1 with ab137132 at a dilution of 1/500. Heat mediated antigen retrieval was performed using EDTA antigen retrieval solution, and microwave treatment for 20 min at 20% power. Anti-Rabbit HRP polymer was used as secondary antibody. Opal tyramide amplification was performed using Opal 540 fluorophore. Counterstained with DAPI stain.
Image scanned with Vectra 3.0 and analyzed via Inform software.
This image was courteously provided by Dr. Houssein Abdul Sater, Georgia Cancer Center.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab137132).
- IHC-P
Unknown
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PD1 antibody [EPR4877(2)] - BSA and Azide free (AB186928)
Immunohistochemical analysis using ab137132 showing negative staining in normal human brain tissue.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab137132).
- IHC-P
Lab
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PD1 antibody [EPR4877(2)] - BSA and Azide free (AB186928)
Immunohistochemistry (Paraffin-embedded sections) analysis of Human tonsil tissue labeling PD1 with ab186928 at 1/1000. Heat mediated antigen retrieval was perfomed using Tris/EDTA buffer pH 9. Anti-rabbit IgG H&L (HRP) (ab97051) was used as the secondary antibody at 1/500. Counterstained with hematoxylin.
- IHC-P
Unknown
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PD1 antibody [EPR4877(2)] - BSA and Azide free (AB186928)
Immunohistochemical (Formalin/PFA-fixed paraffin-embedded sections) analysis of human tonsil tissue labeling PD1 with ab137132 at a dilution of 1/250. Anti-Rabbit HRP polymer was used as the secondary detection system. Heat-mediated antigen retrieval was performed using EDTA based pH 9.0 buffer.
- IHC-P
Unknown
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PD1 antibody [EPR4877(2)] - BSA and Azide free (AB186928)
Immunohistochemical (Formalin/PFA-fixed paraffin-embedded sections) analysis of human tonsil tissue labeling PD1 with ab137132 at a dilution of 1/250. Anti-Rabbit HRP polymer was used as the secondary detection system. Heat-mediated antigen retrieval was performed using citrate based pH 6.0 buffer.
- IHC-P
Unknown
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PD1 antibody [EPR4877(2)] - BSA and Azide free (AB186928)
Clone EPR4877(2) (ab186928) has been successfully conjugated by Abcam. This image was generated using Anti-PD1 antibody [EPR4877(2)] (Alexa Fluor® 647). Please refer to ab201825 for protocol details.
IHC image of PD1 staining in a section of formalin-fixed paraffin-embedded normal human tonsil*.
The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6) in a Dako Pascal pressure cooker using the standard factory-set regime. Non-specific protein-protein interactions were then blocked in TBS containing 0.025% (v/v) Triton X-100, 0.3M (w/v) glycine and 1% (w/v) BSA for 1h at room temperature. The section was then incubated overnight at +4°C in TBS containing 0.025% (v/v) Triton X-100 and 1% (w/v) BSA with ab201825 at 1/100 dilution (shown in red) and counterstained using ab195887, Mouse monoclonal to alpha Tubulin (Alexa Fluor® 488), at 1/250 dilution (shown in green). Nuclear DNA was labelled with DAPI (shown in blue). The section was then mounted using Fluoromount®.
Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).
For other IHC staining systems (automated and non-automated), customers should optimize variable parameters such as antigen retrieval conditions, antibody concentrations and incubation times.
*Tissue obtained from the Human Research Tissue Bank, supported by the NIHR Cambridge Biomedical Research Centre.
- IHC-P
Unknown
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PD1 antibody [EPR4877(2)] - BSA and Azide free (AB186928)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human tonsil tissue labelling PD1 with purified ab137132 at 1/500. Heat mediated antigen retrieval was performed using Tris/EDTA buffer pH 9. ab97051, a HRP-conjugated goat anti-rabbit IgG (H+L) was used as the secondary antibody (1/500). Negative control using PBS instead of primary antibody. Counterstained with hematoxylin.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab137132).
- IHC-P
Supplier Data
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PD1 antibody [EPR4877(2)] - BSA and Azide free (AB186928)
Immunohistochemical analysis of formalin fixed paraffin embedded human tonsil labelling PD-L1 with ab186928 at a concentration of 5µg/ml. The immunostaining was performed on a Ventana DISCOVERY ULTRA (Roche Tissue Diagnostics) instrument with an OptiView DAB IHC Detection Kit. Heat mediated antigen retrieval was conducted for 32min with ULTRA cell conditioning solution (CC1 pH8.5). ab186928 anti PD-L1 antibody was incubated at 37oC for 16min. Sections were counterstained is with Hematoxylin II. Image inset shows absence of staining in secondary antibody only control.
- IHC-P
Unknown
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PD1 antibody [EPR4877(2)] - BSA and Azide free (AB186928)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human tonsil tissue labelling PD1 with unpurified ab137132 at a dilution of 1/250.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab137132).
- IHC-P
Unknown
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PD1 antibody [EPR4877(2)] - BSA and Azide free (AB186928)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human T-cell lymphoma tissue labelling PD1 with unpurified ab137132 at a dilution of 1/250.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab137132).
- IHC-P
Lab
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PD1 antibody [EPR4877(2)] - BSA and Azide free (AB186928)
Tissue Microarrays stained for "Anti-PD1 antibody [EPR4877(2)]" using "ab137132"in immunohistochemical analysis. This table provides a detailed overview of positive (tick mark) and negative (cross mark) staining per sample type tested. The sections were pre-treated using Heat mediated antigen retrieval using Bond™ Epitope Retrieval Solution 2 (pH 9.0) for 20 minutes. The sections were incubated with ab137132 for 30 mins at room temperature followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). The immunostaining was performed on a Leica Biosystems BOND® RX instrument.
Related conjugates and formulations (7)
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603 Alexa Fluor® 568
Alexa Fluor® 568 Anti-PD1 antibody [EPR4877(2)]
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519 Alexa Fluor® 488
Alexa Fluor® 488 Anti-PD1 antibody [EPR4877(2)]
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Anti-PD1 antibody [EPR4877(2)]
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775 Alexa Fluor® 750
Alexa Fluor® 750 Anti-PD1 antibody [EPR4877(2)]
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665 Alexa Fluor® 647
Alexa Fluor® 647 Anti-PD1 antibody [EPR4877(2)]
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565 Alexa Fluor® 555
Alexa Fluor® 555 Anti-PD1 antibody [EPR4877(2)]
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617 Alexa Fluor® 594
Alexa Fluor® 594 Anti-PD1 antibody [EPR4877(2)]
Reactivity data
Product details
What is this antibody validated in?
Anti-PD1 antibody [EPR4877(2)] - BSA and Azide free (ab186928) is a rabbit recombinant monoclonal antibody and is validated for use in Immunohistochemistry (IHC-P) in Human samples.
Other related products
We have a range of other formats of antibody clone [EPR4877(2)] also available for your convenience: ab137132, Carrier free - ab186928, Alexa Fluor® 647 - ab201825, Alexa Fluor® 555 - ab275126, Alexa Fluor® 594 - ab275349, Alexa Fluor® 488 - ab311022, Alexa Fluor® 568 - ab313074, Alexa Fluor® 750 - ab321110
Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
Compatibility
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
Properties and storage information
Form
Purification technique
Storage buffer
Shipped at conditions
Appropriate short-term storage conditions
Appropriate long-term storage conditions
Storage information
Supplementary information
This supplementary information is collated from multiple sources and compiled automatically.
Biological function summary
PD1 serves as an inhibitory receptor acting as a checkpoint in the immune system. It becomes part of an immune-suppressive complex when it binds with its ligands PD-L1 or PD-L2 which are expressed on various cell types including some tumor cells. This interaction suppresses the proliferation of T cells and cytokine production contributing to immune homeostasis. By controlling T cell activity PD1 limits autoimmunity but can also reduce the immune system's capability to attack cancer cells.
Pathways
PD1 functions in the immune checkpoint pathway a critical regulatory circuit in immune regulation. The engagement of PD1 with its ligands initiates a cascade that inhibits the function and proliferation of T cells through downstream SHP-2 phosphatase activity. This pathway frequently involves other regulatory proteins like CTLA-4 and is an important mechanism by which the body modulates immune responses. Related pathways often intersect with those involving T cell receptor signaling and contribute to the overall modulation of immune activity.
Product protocols
- Visit the General protocols
- Visit the Troubleshooting
Target data
Publications (10)
Recent publications for all applications. Explore the full list and refine your search
Nature immunology 26:1182-1197 PubMed40588561
2025
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iScience 27:110550 PubMed39165839
2024
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Cell reports. Medicine 5:101438 PubMed38401548
2024
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Nature communications 14:8435 PubMed38114518
2023
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iScience 26:107891 PubMed37766980
2023
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Frontiers in immunology 14:1162669 PubMed37207208
2023
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Blood cancer journal 13:57 PubMed37088806
2023
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Cancers 15: PubMed36765785
2023
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Cell reports 39:110639 PubMed35385730
2022
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Cancer immunology research 7:644-657 PubMed30745366
2019
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Product promise
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
For licensing inquiries, please contact partnerships@abcam.com