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Mouse anti-PD1 antibody NAT105 ab52587 is a mouse monoclonal antibody that is used in PD1 western blotting, IHC, immunofluorescence and flow cytometry.

Tried and trusted by researchers since 2007
Anti-PD1 antibody clone NAT105 is cited in over 360 publications
One antibody for all your PD1 staining, use in PD1 western blotting, IHC, immunofluorescence and flow cytometry


Images

Flow Cytometry - Anti-PD1 antibody [NAT105] (AB52587), expandable thumbnail
  • Immunohistochemistry (Frozen sections) - Anti-PD1 antibody [NAT105] (AB52587), expandable thumbnail
  • Immunocytochemistry/ Immunofluorescence - Anti-PD1 antibody [NAT105] (AB52587), expandable thumbnail
  • Immunohistochemistry (Frozen sections) - Anti-PD1 antibody [NAT105] (AB52587), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PD1 antibody [NAT105] (AB52587), expandable thumbnail

Publications

Key facts

Isotype

IgG1

Host species

Mouse

Storage buffer

pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Form

Liquid

Clonality

Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
IHC-PFlow CytWBICC/IFIHC-Fr
Human
Tested
Tested
Tested
Tested
Tested

Tested
Tested

Species

Human

Dilution info

1/100

Notes

Perform heat-mediated antigen retrieval before commencing with IHC staining protocol.

Tested
Tested

Species

Human

Dilution info

1/1000

Notes

-

Tested
Tested

Species

Human

Dilution info

1/1000

Notes

-

Tested
Tested

Species

Human

Dilution info

1/100

Notes

We recommend Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) (Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) preadsorbed ab150117) secondary antibody.

Tested
Tested

Species

Human

Dilution info

1/10000

Notes

-

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Target data

Function

Inhibitory receptor on antigen activated T-cells that plays a critical role in induction and maintenance of immune tolerance to self (PubMed:21276005). Delivers inhibitory signals upon binding to ligands CD274/PDCD1L1 and CD273/PDCD1LG2 (PubMed:21276005). Following T-cell receptor (TCR) engagement, PDCD1 associates with CD3-TCR in the immunological synapse and directly inhibits T-cell activation (By similarity). Suppresses T-cell activation through the recruitment of PTPN11/SHP-2: following ligand-binding, PDCD1 is phosphorylated within the ITSM motif, leading to the recruitment of the protein tyrosine phosphatase PTPN11/SHP-2 that mediates dephosphorylation of key TCR proximal signaling molecules, such as ZAP70, PRKCQ/PKCtheta and CD247/CD3zeta (By similarity).The PDCD1-mediated inhibitory pathway is exploited by tumors to attenuate anti-tumor immunity and escape destruction by the immune system, thereby facilitating tumor survival (PubMed:28951311). The interaction with CD274/PDCD1L1 inhibits cytotoxic T lymphocytes (CTLs) effector function (PubMed:28951311). The blockage of the PDCD1-mediated pathway results in the reversal of the exhausted T-cell phenotype and the normalization of the anti-tumor response, providing a rationale for cancer immunotherapy (PubMed:22658127, PubMed:25034862, PubMed:25399552).

Alternative names

Programmed cell death protein 1, Protein PD-1, hPD-1, PD1, PDCD1

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Mouse anti-PD1 antibody NAT105 ab52587 is a mouse monoclonal antibody that is used in PD1 western blotting, IHC, immunofluorescence and flow cytometry.

Tried and trusted by researchers since 2007
Anti-PD1 antibody clone NAT105 is cited in over 360 publications
One antibody for all your PD1 staining, use in PD1 western blotting, IHC, immunofluorescence and flow cytometry

Alternative names

Programmed cell death protein 1, Protein PD-1, hPD-1, PD1, PDCD1

Key facts

Isotype

IgG1

Form

Liquid

Clonality

Monoclonal

Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Clone number

NAT105

Purification technique

Affinity purification Protein A

Light chain type

kappa

Concentration
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Storage

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

Notes

Please note that PD-1 is expressed variably in different tissues and that optimisation may be required depending on the tissue used for the experiment.

Western blot protocol advice:

Due to low expression of PD-1, we recommend loading a high amount of sample (100 µg) to detect the band for PD-1. Human tonsil and YT cell line lysates are suitable positive controls.

This product has switched from a hybridoma to recombinant production method on 1st November 2024.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.

Activity summary

Programmed cell death protein 1 commonly known as PD-1 or PDCD1 is an immune checkpoint receptor with a molecular mass of approximately 55 kDa. This protein is primarily expressed on the surface of activated T cells B cells and myeloid cells within the immune system. PD-1 plays a mechanical role in modulating immune responses by binding to its ligands PD-L1 and PD-L2 which are expressed on other immune cells and in various other tissues. The engagement of PD-1 with its ligands transmits an inhibitory signal that reduces the proliferation and activity of T cells.

Biological function summary

PD-1 serves as a critical immune checkpoint that helps maintain self-tolerance and prevent autoimmunity. PD-1 does not function as part of a multi-protein complex but independently regulates the immune response. By delivering inhibitory signals upon ligand binding PD-1 limits the overactivation of the immune system reducing the likelihood of tissue damage during inflammatory responses. The modulation of T cell activity by PD-1 contributes to a balanced immune system ensuring that the body targets pathogens effectively without harming itself.

Pathways

PD-1 interacts with key immune-regulatory pathways including the PI3K-Akt and Ras-MAPK pathways. These pathways are important for cell survival growth and metabolism. The interaction of PD-1 with the PI3K-Akt pathway involves proteins such as SHP-2 which dephosphorylates signaling intermediates leading to reduced T cell receptor signaling. PD-1's role in these pathways demonstrates its influence on immune cell function particularly in regulating the intensity and duration of immune responses.

Associated diseases and disorders

The PD-1 pathway plays an important role in cancer and autoimmune diseases. Tumors often exploit the PD-1 pathway to evade immune surveillance leading to cancer progression. In such cases blocking PD-1 with inhibitors like anti-PD-1 antibodies (e.g. EH12.2H7 and chimeric antibodies) can re-activate T cells against cancer cells. Conversely in autoimmune diseases heightened PD-1 expression or activity could help in moderating aberrant immune responses where the immune system attacks the body's own tissues. The PD-1 pathway interacting with proteins such as CTLA-4 in cancer and autoimmune contexts represents a target for therapeutics aiming to modulate the immune system to improve disease outcomes.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

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7 product images

  • Flow Cytometry - Anti-PD1 antibody [NAT105] (ab52587), expandable thumbnail

    Flow Cytometry - Anti-PD1 antibody [NAT105] (ab52587)

    Cells were fixed with 100?l 4% PFA for 10 min at RT, then permeabilized with 100?l 90% methanol for 30 min (-20° C) followed by blocking with 10% goat serum for 1h at room temperature. Incubate ab52587 for 30 min at room temperature (RT) and then incubate Goat anti-Mouse IgG (Alexa Fluor® 488, Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) ab150113) for 30 min at room temperature. Positive staining on MOLT-4 treated with 10ng/ml PMA and 500ng/ml Ionomycin for 24h, but weak staining on untreated MOLT-4.

  • Immunohistochemistry (Frozen sections) - Anti-PD1 antibody [NAT105] (ab52587), expandable thumbnail

    Immunohistochemistry (Frozen sections) - Anti-PD1 antibody [NAT105] (ab52587)

    Immunohistochemical analysis of permeabilized frozen Human tonsil tissue labeling PD1 with Anti-Agrin antibody [EPR28044-74] ab316965 at 1/10000 dilution, followed by LeicaDS9800 (Bond™ PolymerRefine Detection). Counterstained with hematoxylin. The immunostaining was performed on a Leica Biosystems BOND® RX instrument.

  • Immunocytochemistry/ Immunofluorescence - Anti-PD1 antibody [NAT105] (ab52587), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Anti-PD1 antibody [NAT105] (ab52587)

    Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized MOLT-4 cells labelling PD1 with ab52587 at 1/100 dilution, followed by Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) preadsorbed (Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) preadsorbed ab150117) at 1/1000 dilution at RT for 45 min. Recombinant Alexa Fluor® 594 Anti-beta Tubulin antibody [EPR16774] (Alexa Fluor® 594 Anti-beta Tubulin antibody [EPR16774] ab206369) was used as a counterstain at 1/50 dilution and was co-incubated with ab52587 overnight at 4° C. Nucleus were visualized using DAPI. Confocal image showing membranous staining in MOLT-4 cells treated with 500 ng/ml Ionomycin and 10 ng/ml Phorbol-12-myristate-13-acetate (PMA) for 24 hours, and no staining in MOLT-4 cells.

  • Immunohistochemistry (Frozen sections) - Anti-PD1 antibody [NAT105] (ab52587), expandable thumbnail

    Immunohistochemistry (Frozen sections) - Anti-PD1 antibody [NAT105] (ab52587)

    Immunohistochemical analysis of permeabilized frozen Human cardiac muslce tissue labeling PD1 with Anti-Agrin antibody [EPR28044-74] ab316965 at 1/10000 dilution, followed by LeicaDS9800 (Bond™ PolymerRefine Detection). No staining on human cardiac muscle. Counterstained with hematoxylin. The immunostaining was performed on a Leica Biosystems BOND® RX instrument.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PD1 antibody [NAT105] (ab52587), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PD1 antibody [NAT105] (ab52587)

    Immunohistochemical analysis of paraffin-embedded Human tonsil tissue labeling PD1 with ab52587 at 1/100 dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin. Heat mediated antigen retrieval was performed with Citrate buffer (pH 6.0, Epitope Retrieval Solution 1) for 20 mins.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PD1 antibody [NAT105] (ab52587), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PD1 antibody [NAT105] (ab52587)

    Immunohistochemical analysis of paraffin-embedded Human cardiac muscle tissue labeling PD1 with ab52587 at 1/100 dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). No staining on human cardiac muscle. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin. Heat mediated antigen retrieval was performed with Citrate buffer (pH 6.0, Epitope Retrieval Solution 1) for 20 mins.

  • Western blot - Anti-PD1 antibody [NAT105] (ab52587), expandable thumbnail

    Western blot - Anti-PD1 antibody [NAT105] (ab52587)

    Blocking and diluting buffer and concentration: 5% NFDM/TBST.

    Lane 1: Untreated MOLT-4 (human lymphoblastic leukemia T lymphoblast) whole cell lysate at 20 µg

    Lane 2: MOLT-4 treated with 500ng/ml Ionomycin and 10ng/ml Phorbol-12-myristate-13-acetate (PMA) for 24 hours, whole cell lysate at 20 µg

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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