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Mouse Monoclonal PD1 antibody. Carrier free. Suitable for IHC-P, WB, ICC/IF, Flow Cyt (Intra), Flow Cyt and reacts with Human samples. Cited in 1 publication.

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Images

Flow Cytometry - Anti-PD1 antibody [NAT105] - BSA and Azide free (AB201811), expandable thumbnail
  • Immunohistochemistry (Frozen sections) - Anti-PD1 antibody [NAT105] - BSA and Azide free (AB201811), expandable thumbnail
  • Immunohistochemistry (Frozen sections) - Anti-PD1 antibody [NAT105] - BSA and Azide free (AB201811), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PD1 antibody [NAT105] - BSA and Azide free (AB201811), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PD1 antibody [NAT105] - BSA and Azide free (AB201811), expandable thumbnail

Publications

Key facts

Isotype
IgG1
Host species
Mouse
Storage buffer

pH: 7.2 - 7.4
Constituents: PBS

Form
Liquid
Clonality
Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
IHC-PWBICC/IFFlow Cyt (Intra)Flow Cyt
Human
Tested
Tested
Tested
Tested
Tested

Tested
Tested

Species
Human
Dilution info
-
Notes

Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Tested
Tested

Species
Human
Dilution info
-
Notes

For WB protocol, see Protocols section of the website

Tested
Tested

Species
Human
Dilution info
-
Notes

-

Tested
Tested

Species
Human
Dilution info
-
Notes

-

Tested
Tested

Species
Human
Dilution info
-
Notes

-

Associated Products

Select an associated product type

8 products for Alternative Product

3 products for Alternative Version

Target data

Function

Inhibitory receptor on antigen activated T-cells that plays a critical role in induction and maintenance of immune tolerance to self (PubMed:21276005). Delivers inhibitory signals upon binding to ligands CD274/PDCD1L1 and CD273/PDCD1LG2 (PubMed:21276005). Following T-cell receptor (TCR) engagement, PDCD1 associates with CD3-TCR in the immunological synapse and directly inhibits T-cell activation (By similarity). Suppresses T-cell activation through the recruitment of PTPN11/SHP-2: following ligand-binding, PDCD1 is phosphorylated within the ITSM motif, leading to the recruitment of the protein tyrosine phosphatase PTPN11/SHP-2 that mediates dephosphorylation of key TCR proximal signaling molecules, such as ZAP70, PRKCQ/PKCtheta and CD247/CD3zeta (By similarity). The PDCD1-mediated inhibitory pathway is exploited by tumors to attenuate anti-tumor immunity and escape destruction by the immune system, thereby facilitating tumor survival (PubMed:28951311). The interaction with CD274/PDCD1L1 inhibits cytotoxic T lymphocytes (CTLs) effector function (PubMed:28951311). The blockage of the PDCD1-mediated pathway results in the reversal of the exhausted T-cell phenotype and the normalization of the anti-tumor response, providing a rationale for cancer immunotherapy (PubMed:22658127, PubMed:25034862, PubMed:25399552).

Alternative names

CD279, PD1, PDCD1, Programmed cell death protein 1, Protein PD-1, hPD-1

Recommended products

Mouse Monoclonal PD1 antibody. Carrier free. Suitable for IHC-P, WB, ICC/IF, Flow Cyt (Intra), Flow Cyt and reacts with Human samples. Cited in 1 publication.

Key facts

Isotype
IgG1
Form
Liquid
Clonality
Monoclonal
Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Carrier free
Yes
Clone number
NAT105
Purification technique
Affinity purification Protein G
Light chain type
kappa
Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
Do Not Freeze

Notes

ab201811 is the carrier-free version of Anti-PD1 antibody [NAT105] ab52587.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.
Activity summary

PD1 also known as Programmed Cell Death Protein 1 or PDCD1 is a transmembrane protein that plays a critical role in regulating immune responses. It has a mass of approximately 55 kDa. PD1 is expressed on the surface of T cells B cells and some myeloid cells. PD1’s expression increases upon activation of these immune cells assisting in maintaining peripheral tolerance. Researchers often use PD1 mouse models and chimeric antibodies to explore the function of PD1 for experimental purposes. Antibodies such as anti-PD1 such as EH12.2H7 help in blocking PD1 interaction to study its role further.

Biological function summary

PD1 serves as an inhibitory receptor acting as a checkpoint in the immune system. It becomes part of an immune-suppressive complex when it binds with its ligands PD-L1 or PD-L2 which are expressed on various cell types including some tumor cells. This interaction suppresses the proliferation of T cells and cytokine production contributing to immune homeostasis. By controlling T cell activity PD1 limits autoimmunity but can also reduce the immune system's capability to attack cancer cells.

Pathways

PD1 functions in the immune checkpoint pathway a critical regulatory circuit in immune regulation. The engagement of PD1 with its ligands initiates a cascade that inhibits the function and proliferation of T cells through downstream SHP-2 phosphatase activity. This pathway frequently involves other regulatory proteins like CTLA-4 and is an important mechanism by which the body modulates immune responses. Related pathways often intersect with those involving T cell receptor signaling and contribute to the overall modulation of immune activity.

Associated diseases and disorders

PD1 has a significant role in cancer and autoimmune disorders. PD1 expression can allow tumors to evade immune surveillance making PD1 a target for cancer therapies such as anti-PD1 antibodies which aim to block PD1 and restore T cell activity. The interaction of PD1 with cancer-related proteins like PD-L1 facilitates tumor immune evasion. In autoimmune disorders PD1’s regulation of immune balance can become dysregulated leading to persistent immune activation and tissue damage. Understanding PD1 and its interaction with proteins such as PD-L1 helps in developing therapeutic strategies for both cancer and autoimmune conditions.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
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7 product images

  • Flow Cytometry - Anti-PD1 antibody [NAT105] - BSA and Azide free (ab201811), expandable thumbnail

    Flow Cytometry - Anti-PD1 antibody [NAT105] - BSA and Azide free (ab201811)

    PD1 Flow Cytometry staining of MOLT-4 treated with 10ng/ml PMA and 500ng/ml Ionomycin using mouse Anti-PD1 antibody

    Cells were fixed with 100µl 4% PFA for 10 min at RT, then permeabilized with 100µl 90% methanol for 30 min (-20° C) followed by blocking with 10% goat serum for 1h at room temperature. Incubate Anti-PD1 antibody [NAT105] ab52587 for 30 min at room temperature (RT) and then incubate Goat anti-Mouse IgG (Alexa Fluor® 488, Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) ab150113) for 30 min at room temperature. Positive staining on MOLT-4 treated with 10ng/ml PMA and 500ng/ml Ionomycin for 24h, but weak staining on untreated MOLT-4.

    This data was developed using the same antibody clone in a different buffer formulation containing BSA and sodium azide (Anti-PD1 antibody [NAT105] ab52587).

  • Immunohistochemistry (Frozen sections) - Anti-PD1 antibody [NAT105] - BSA and Azide free (ab201811), expandable thumbnail

    Immunohistochemistry (Frozen sections) - Anti-PD1 antibody [NAT105] - BSA and Azide free (ab201811)

    PD1 Immunohistochemistry (Frozen sections) staining of Human cardiac muscle tissue using mouse Anti-PD1 antibody

    Immunohistochemical analysis of permeabilized frozen Human cardiac muslce tissue labeling PD1 with Anti-Agrin antibody [EPR28044-74] ab316965 at 1/10000 dilution, followed by LeicaDS9800 (Bond™ PolymerRefine Detection). No staining on human cardiac muscle. Counterstained with hematoxylin. The immunostaining was performed on a Leica Biosystems BOND® RX instrument.

    This data was developed using the same antibody clone in a different buffer formulation containing BSA and sodium azide (Anti-PD1 antibody [NAT105] ab52587).

  • Immunohistochemistry (Frozen sections) - Anti-PD1 antibody [NAT105] - BSA and Azide free (ab201811), expandable thumbnail

    Immunohistochemistry (Frozen sections) - Anti-PD1 antibody [NAT105] - BSA and Azide free (ab201811)

    PD1 Immunohistochemistry (Frozen sections) staining of Human tonsil tissue using mouse Anti-PD1 antibody

    Immunohistochemical analysis of permeabilized frozen Human tonsil tissue labeling PD1 with Anti-Agrin antibody [EPR28044-74] ab316965 at 1/10000 dilution, followed by LeicaDS9800 (Bond™ PolymerRefine Detection). Counterstained with hematoxylin. The immunostaining was performed on a Leica Biosystems BOND® RX instrument.

    This data was developed using the same antibody clone in a different buffer formulation containing BSA and sodium azide (Anti-PD1 antibody [NAT105] ab52587).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PD1 antibody [NAT105] - BSA and Azide free (ab201811), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PD1 antibody [NAT105] - BSA and Azide free (ab201811)

    PD1 Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) staining of Human cardiac muscle tissue using mouse Anti-PD1 antibody

    Immunohistochemical analysis of paraffin-embedded Human cardiac muscle tissue labeling PD1 with Anti-PD1 antibody [NAT105] ab52587 at 1/100 dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). No staining on human cardiac muscle. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin. Heat mediated antigen retrieval was performed with Citrate buffer (pH 6.0, Epitope Retrieval Solution 1) for 20 mins.

    This data was developed using the same antibody clone in a different buffer formulation containing BSA and sodium azide (Anti-PD1 antibody [NAT105] ab52587).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PD1 antibody [NAT105] - BSA and Azide free (ab201811), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PD1 antibody [NAT105] - BSA and Azide free (ab201811)

    PD1 Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) staining of Human tonsil tissue using mouse Anti-PD1 antibody

    Immunohistochemical analysis of paraffin-embedded Human tonsil tissue labeling PD1 with Anti-PD1 antibody [NAT105] ab52587 at 1/100 dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin. Heat mediated antigen retrieval was performed with Citrate buffer (pH 6.0, Epitope Retrieval Solution 1) for 20 mins.

    This data was developed using the same antibody clone in a different buffer formulation containing BSA and sodium azide (Anti-PD1 antibody [NAT105] ab52587).

  • Immunocytochemistry/ Immunofluorescence - Anti-PD1 antibody [NAT105] - BSA and Azide free (ab201811), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Anti-PD1 antibody [NAT105] - BSA and Azide free (ab201811)

    PD1 Immunocytochemistry/ Immunofluorescence staining of MOLT-4 treated with 10ng/ml PMA and 500ng/ml Ionomycin using mouse Anti-PD1 antibody

    Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized MOLT-4 cells labelling PD1 with Anti-PD1 antibody [NAT105] ab52587 at 1/100 dilution, followed by Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) preadsorbed (Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) preadsorbed ab150117) at 1/1000 dilution at RT for 45 min. Recombinant Alexa Fluor® 594 Anti-beta Tubulin antibody [EPR16774] (Alexa Fluor® 594 Anti-beta Tubulin antibody [EPR16774] - Microtubule Marker ab206369) was used as a counterstain at 1/50 dilution and was co-incubated with Anti-PD1 antibody [NAT105] ab52587 overnight at 4° C. Nucleus were visualized using DAPI. Confocal image showing membranous staining in MOLT-4 cells treated with 500 ng/ml Ionomycin and 10 ng/ml Phorbol-12-myristate-13-acetate (PMA) for 24 hours, and no staining in MOLT-4 cells.

    This data was developed using the same antibody clone in a different buffer formulation containing BSA and sodium azide (Anti-PD1 antibody [NAT105] ab52587).

  • Western blot - Anti-PD1 antibody [NAT105] - BSA and Azide free (ab201811), expandable thumbnail

    Western blot - Anti-PD1 antibody [NAT105] - BSA and Azide free (ab201811)

    PD1 Western blot staining using mouse Anti-PD1 antibody

    Blocking and diluting buffer and concentration: 5% NFDM/TBST.

    This data was developed using the same antibody clone in a different buffer formulation containing BSA and sodium azide (Anti-PD1 antibody [NAT105] ab52587).

    All lanes: Western blot - Anti-PD1 antibody [NAT105] (Anti-PD1 antibody [NAT105] ab52587) at 1/1000 dilution

    Lane 1: Untreated MOLT-4 (human lymphoblastic leukemia T lymphoblast) whole cell lysate at 20 µg

    Lane 2: MOLT-4 treated with 500ng/ml Ionomycin and 10ng/ml Phorbol-12-myristate-13-acetate (PMA) for 24 hours, whole cell lysate at 20 µg

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

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