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AB324608

Anti-PD1 (phospho Y248) antibody [407.4.6G12.20.13.2]

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Mouse Recombinant Monoclonal PD1 phospho Y248 antibody. Suitable for WB, Flow Cyt (Intra), Dot and reacts with Transfected cell lysate - Human, Transfected cell line - Human, Synthetic peptide - Human samples.

View Alternative Names

CD279, PD1, PDCD1, Programmed cell death protein 1, Protein PD-1, hPD-1

3 Images
Flow Cytometry (Intracellular) - Anti-PD1 (phospho Y248) antibody [407.4.6G12.20.13.2] (AB324608)
  • Flow Cyt (Intra)

Supplier Data

Flow Cytometry (Intracellular) - Anti-PD1 (phospho Y248) antibody [407.4.6G12.20.13.2] (AB324608)

Flow cytometric analysis of 4% paraformaldehyde fixed 90% methanol permeabilized Isotype (Upper left) / Untreated 293T (human embryonic kidney epithelial cell) transfected with Programmed cell death protein 1 protein expression vector containing a myc-His-tag® (Upper middle) / 293T transfected with Programmed cell death protein 1 protein expression vector containing a myc-His-tag® treated with Pervanadate (100uM) for 30 mins (Upper right) / Untreated 293T transfected with Programmed cell death protein 1 (Y248F) protein expression vector containing a myc-His-tag® (Lower left) / 293T transfected with Programmed cell death protein 1 (Y248F) protein expression vector containing a myc-His-tag® treated with Pervanadate (100uM) for 30 mins (Lower middle) / 293T transfected with emptor vector containing a myc-His-tag® (Lower right) cells labelling PD1 (phospho Y248) with ab324608 at 1/500 dilution (0.1ug) compared with a Mouse monoclonal IgG / Left isotype control.

Goat anti-Mouse IgG (Alexa Fluor® 488, ab150113) at 1/5000 dilution was used as the secondary antibody.

Western blot - Anti-PD1 (phospho Y248) antibody [407.4.6G12.20.13.2] (AB324608)
  • WB

Supplier Data

Western blot - Anti-PD1 (phospho Y248) antibody [407.4.6G12.20.13.2] (AB324608)

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (ab181602) staining at 1/200000 dilution.

In Western blot, Anti-6X His tag® antibody [EPR20547] - ChIP Grade (ab213204) staining at 1/5000 dilution.

All lanes:

Western blot - Anti-PD1 (phospho Y248) antibody [407.4.6G12.20.13.2] (ab324608) at 1/1000 dilution

Lane 1:

293T cells transfected with an empty vector containing a myc-his-tag, whole cell lysate at 20 µg

Lane 2:

293T transfected with a human PDCD1 expression vector containing a myc-his-tag, whole cell lysate at 20 µg

Lane 3:

293T transfected with a human PDCD1 expression vector containing a myc-his-tag were treated with 100uM Pervanadate for 30 mins, whole cell lysate at 20 µg

Lane 4:

293T transfected with a human PDCD1 (mutation) expression vector containing a myc-His-tag, whole cell lysate at 20 µg

Lane 5:

293T transfected with a human PDCD1 (mutation) expression vector containing a myc-his-tag were treated with 100uM Pervanadate for 30 mins, whole cell lysate at 20 µg

Secondary

All lanes:

Peroxidase-Conjugated Goat anti-Mouse IgG (H+L) at 1/10000 dilution

Observed band size: 55 kDa,36 kDa

false

Exposure time: 6s

Dot Blot - Anti-PD1 (phospho Y248) antibody [407.4.6G12.20.13.2] (AB324608)
  • Dot

Supplier Data

Dot Blot - Anti-PD1 (phospho Y248) antibody [407.4.6G12.20.13.2] (AB324608)

Dot blot analysis of PD1 (phospho Y248) using ab324608 at 1 : 1000 followed by a Peroxidase-Conjugated Goat anti-Mouse IgG (H+L) at 1 : 10,000 dilution.

Lane1 : Human PDCD1 (phospho Y248) peptide
Lane2 : Human PDCD1 non-phospho peptide

Exposure time : 180 seconds.

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

All lanes:

Dot Blot - Anti-PD1 (phospho Y248) antibody [407.4.6G12.20.13.2] (ab324608) at 1/1000 dilution

Lane 1:

Human PDCD1 (phospho Y248) peptide

Lane 2:

Human PDCD1 non-phospho peptide

Secondary

All lanes:

Peroxidase-Conjugated Goat anti-Mouse IgG (H+L) at 1/10000 dilution

false

Exposure time: 180s

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

407.4.6G12.20.13.2

Isotype

IgG2a

Carrier free

No

Reacts with

Human

Applications

WB, Dot, Flow Cyt (Intra)

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

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Product details

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Want a custom formulation?
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Inhibitory receptor on antigen activated T-cells that plays a critical role in induction and maintenance of immune tolerance to self (PubMed : 21276005, PubMed : 31754127, PubMed : 32184441, PubMed : 37208329). Delivers inhibitory signals upon binding to ligands CD274/PDCD1L1 and CD273/PDCD1LG2 (PubMed : 21276005, PubMed : 26602187). Following T-cell receptor (TCR) engagement, PDCD1 associates with TCR-CD3 in the immunological synapse and directly inhibits T-cell activation (PubMed : 32184441). Suppresses T-cell activation through the recruitment of PTPN11/SHP-2 : following ligand-binding, PDCD1 is phosphorylated within the ITSM motif, leading to the recruitment of the protein tyrosine phosphatase PTPN11/SHP-2 that mediates dephosphorylation of key TCR proximal signaling molecules, such as ZAP70, PRKCQ/PKCtheta and CD247/CD3zeta (PubMed : 32184441).. The PDCD1-mediated inhibitory pathway is exploited by tumors to attenuate anti-tumor immunity and escape destruction by the immune system, thereby facilitating tumor survival (PubMed : 28951311). The interaction with CD274/PDCD1L1 inhibits cytotoxic T lymphocytes (CTLs) effector function (PubMed : 28951311). The blockage of the PDCD1-mediated pathway results in the reversal of the exhausted T-cell phenotype and the normalization of the anti-tumor response, providing a rationale for cancer immunotherapy (PubMed : 22658127, PubMed : 25034862, PubMed : 25399552).
See full target information PDCD1 pY248
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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