AB206378

Anti-PD1 (phospho Y248) antibody [EPR19821]

20ul selling size
RabMAb
Recombinant
What is this?

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(2 Publications)

Anti-PD1 (phospho Y248) antibody [EPR19821] (ab206378) is a rabbit monoclonal antibody detecting PD1 in Western Blot, IP, Dot Blot. Suitable for Human.

- Biophysical QC for unrivalled batch-batch consistency

View Alternative Names

CD279, PD1, PDCD1, Programmed cell death protein 1, Protein PD-1, hPD-1

3 Images

Supplier Data

Immunoprecipitation - Anti-PD1 (phospho Y248) antibody [EPR19821] (AB206378)

PD1 (phospho Y248) was immunoprecipitated from 0.35 mg of HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) transfected with a PD1 (WT) plus 25 kDa fusion protein expression vector, then treated with 100 μM pervanadate for 10 minutes whole cell lysate, with ab206378 at 1/30 dilution. Western blot was performed from the immunoprecipitate using ab206378 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366) was used for detection at 1/5000 dilution.

Lane 1 : HEK-293T transfected with a PD1 (WT) plus 25 kDa fusion protein expression vector, then treated with 100 μM pervanadate for 10 minutes whole cell lysate 10 μg (Input).

Lane 2 : ab206378 IP in HEK-293T transfected with a PD1 (WT) plus 25 kDa fusion protein expression vector, then treated with 100 μM pervanadate for 10 minutes whole cell lysate.

Lane 3 : Rabbit monoclonal IgG (ab172730) instead of ab206378 in HEK-293T transfected with a PD1 (WT) plus 25 kDa fusion protein expression vector, then treated with 100 μM pervanadate for 10 minutes whole cell lysate.

Exposure time : 30 seconds
Blocking and diluting buffer and concentration : 5% NFDM/TBST

The observed bands around 75 kDa comprise PD-1 plus the 25 kDa fusion protein. The plasmids were kindly provided by our collaborator Dr. Liang Chen, NIBS.

All lanes:

Immunoprecipitation - Anti-PD1 (phospho Y248) antibody [EPR19821] (ab206378)

Predicted band size: 32 kDa

Observed band size: 70-75 kDa

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Supplier Data

Western blot - Anti-PD1 (phospho Y248) antibody [EPR19821] (AB206378)

Blocking/Dilution buffer : 5% NFDM/TBST.

The observed bands at around 75 kDa are PD-1 plus the 25 kDa fusion protein. The expression profile observed is consistent with what has been described in the literature (PMID : 22641383). The plasmids were kindly provided by our collaborator Dr. Liang Chen NIBS.

All lanes:

Western blot - Anti-PD1 (phospho Y248) antibody [EPR19821] (ab206378) at 1/1000 dilution

Lane 1:

Untreated-HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate transfected with a PD1 (WT) plus a 25 kDa fusion protein expression vector at 20 µg

Lane 2:

HEK-293T transfected with a PD1 (WT) plus a 25 kDa fusion protein expression vector, then treated with 100 μM pervanadate for 10 minutes whole cell lysate at 20 µg

Lane 3:

HEK-293T transfected with PD1 (WT) plus a 25 kDa fusion protein expression vector, then treated with 100 μM pervanadate for 10 minutes, whole cell lysate (20 μg). Following the transfer to PVDF, the membrane was treated with alkaline phosphatase for 1h at 37°C. at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Predicted band size: 32 kDa

Observed band size: 70-75 kDa

false

Exposure time: 30s

Supplier Data

Dot Blot - Anti-PD1 (phospho Y248) antibody [EPR19821] (AB206378)

Dot blot analysis of PD1 (phospho Y248) labeled with ab206378 at 1/1000 dilution.

Lane 1 : PD1 (phospho Y248) peptide.
Lane 2 : PD1 non-phospho peptide.

Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution was used as secondary antibody.

Blocking/Dilution buffer : 5% NFDM/TBST.

Exposure time : 1 minute.

The blot was developed on a BIO-RAD^® ChemiDoc™ MP instrument.

Carrier free

Anti-PD1 (phospho Y248) antibody [EPR19821] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR19821

Isotype

IgG

Carrier free

Reacts with

Human

Applications

WB, IP, Dot

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "Dot" : {"fullname" : "Dot Blot", "shortname":"Dot"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "1/30", "IP-species-notes": "", "Dot-species-checked": "guaranteed", "Dot-species-dilution-info": "", "Dot-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "" }, "Synthetic peptide - Human": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "Dot-species-checked": "testedAndGuaranteed", "Dot-species-dilution-info": "1/1000", "Dot-species-notes": "", "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "" } } }

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Product details

What is this antibody validated in?
Anti-PD1 (phospho Y248) antibody [EPR19821] (ab206378) is a rabbit recombinant monoclonal antibody and is validated for use in Western Blot (WB), Immunoprecipitation (IP), Dot Blot in Human samples.

What is the molecular weight of PD1?
Anti-PD1 (phospho Y248) [EPR19821] (ab206378) specifically detects a band for PD1 (UniProt: Q15116) at a molecular weight of 32kDa.

Trial sizes available!
Test your antibody or perform pre-screening before committing to a larger quantity. Sold in 10µl. Discover our selection of trial-size antibodies.

Other related products
We have a range of other formats of antibody clone [EPR19821] also available for your convenience: ab206378, Carrier free - ab234960

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

PD1 also known as Programmed Cell Death Protein 1 or PDCD1 is a transmembrane protein that plays a critical role in regulating immune responses. It has a mass of approximately 55 kDa. PD1 is expressed on the surface of T cells B cells and some myeloid cells. PD1's expression increases upon activation of these immune cells assisting in maintaining peripheral tolerance. Researchers often use PD1 mouse models and chimeric antibodies to explore the function of PD1 for experimental purposes. Antibodies such as anti-PD1 such as EH12.2H7 help in blocking PD1 interaction to study its role further.

Biological function summary

PD1 serves as an inhibitory receptor acting as a checkpoint in the immune system. It becomes part of an immune-suppressive complex when it binds with its ligands PD-L1 or PD-L2 which are expressed on various cell types including some tumor cells. This interaction suppresses the proliferation of T cells and cytokine production contributing to immune homeostasis. By controlling T cell activity PD1 limits autoimmunity but can also reduce the immune system's capability to attack cancer cells.

Pathways

PD1 functions in the immune checkpoint pathway a critical regulatory circuit in immune regulation. The engagement of PD1 with its ligands initiates a cascade that inhibits the function and proliferation of T cells through downstream SHP-2 phosphatase activity. This pathway frequently involves other regulatory proteins like CTLA-4 and is an important mechanism by which the body modulates immune responses. Related pathways often intersect with those involving T cell receptor signaling and contribute to the overall modulation of immune activity.

PD1 has a significant role in cancer and autoimmune disorders. PD1 expression can allow tumors to evade immune surveillance making PD1 a target for cancer therapies such as anti-PD1 antibodies which aim to block PD1 and restore T cell activity. The interaction of PD1 with cancer-related proteins like PD-L1 facilitates tumor immune evasion. In autoimmune disorders PD1's regulation of immune balance can become dysregulated leading to persistent immune activation and tissue damage. Understanding PD1 and its interaction with proteins such as PD-L1 helps in developing therapeutic strategies for both cancer and autoimmune conditions.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Inhibitory receptor on antigen activated T-cells that plays a critical role in induction and maintenance of immune tolerance to self (PubMed : 21276005, PubMed : 31754127, PubMed : 32184441, PubMed : 37208329). Delivers inhibitory signals upon binding to ligands CD274/PDCD1L1 and CD273/PDCD1LG2 (PubMed : 21276005, PubMed : 26602187). Following T-cell receptor (TCR) engagement, PDCD1 associates with TCR-CD3 in the immunological synapse and directly inhibits T-cell activation (PubMed : 32184441). Suppresses T-cell activation through the recruitment of PTPN11/SHP-2 : following ligand-binding, PDCD1 is phosphorylated within the ITSM motif, leading to the recruitment of the protein tyrosine phosphatase PTPN11/SHP-2 that mediates dephosphorylation of key TCR proximal signaling molecules, such as ZAP70, PRKCQ/PKCtheta and CD247/CD3zeta (PubMed : 32184441).. The PDCD1-mediated inhibitory pathway is exploited by tumors to attenuate anti-tumor immunity and escape destruction by the immune system, thereby facilitating tumor survival (PubMed : 28951311). The interaction with CD274/PDCD1L1 inhibits cytotoxic T lymphocytes (CTLs) effector function (PubMed : 28951311). The blockage of the PDCD1-mediated pathway results in the reversal of the exhausted T-cell phenotype and the normalization of the anti-tumor response, providing a rationale for cancer immunotherapy (PubMed : 22658127, PubMed : 25034862, PubMed : 25399552).

See full target information PDCD1 phospho Y248

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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

Genetics 225: PubMed37477881

2023

Sleep need, the key regulator of sleep homeostasis, is indicated and controlled by phosphorylation of threonine 221 in salt-inducible kinase 3.

Applications

Unspecified application

Species

Unspecified reactive species

Yang Li,Chengang Li,Yuxiang Liu,Jianjun Yu,Jingqun Yang,Yunfeng Cui,Tao V Wang,Chaoyi Li,Lifen Jiang,Meilin Song,Yi Rao

American journal of reproductive immunology (New York, N.Y. : 1989) 89:e13649 PubMed36394352

2022

Expression and T cell regulatory action of the PD-1 immune checkpoint in the ovary and fallopian tube.

Applications

Unspecified application

Species

Unspecified reactive species

Joshua Johnson,So-Youn Kim,Peter Ka Sam,Rengasamy Asokan,Evelyn Llerena Cari,Elise S Bales,Thanh-Ha Luu,Lauren Perez,Amanda N Kallen,Liesl Nel-Themaat,Alex J Polotsky,Miriam D Post,David J Orlicky,Kimberly R Jordan,Benjamin G Bitler

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-PD1 (phospho Y248) antibody [EPR19821]

Immunoprecipitation - Anti-PD1 (phospho Y248) antibody [EPR19821] (AB206378)

Western blot - Anti-PD1 (phospho Y248) antibody [EPR19821] (AB206378)

Dot Blot - Anti-PD1 (phospho Y248) antibody [EPR19821] (AB206378)

Related conjugates and formulations (1)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

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Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (2)

Product promise