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Anti-PD1 (phospho Y248) antibody [EPR19821] (ab206378) is a rabbit monoclonal antibody detecting PD1 in Western Blot, IP, Dot Blot. Suitable for Human.



- Biophysical QC for unrivalled batch-batch consistency

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Images

Immunoprecipitation - Anti-PD1 (phospho Y248) antibody [EPR19821] (AB206378), expandable thumbnail
  • Western blot - Anti-PD1 (phospho Y248) antibody [EPR19821] (AB206378), expandable thumbnail
  • Dot Blot - Anti-PD1 (phospho Y248) antibody [EPR19821] (AB206378), expandable thumbnail

Publications

Key facts

Isotype
IgG
Host species
Rabbit
Storage buffer

pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Form
Liquid
Clonality
Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
IPDotWB
Human
Tested
Expected
Tested
Synthetic peptide - Human
Not recommended
Tested
Not recommended

Tested
Tested

Species
Human
Dilution info
1/30
Notes

-

Not recommended
Not recommended

Species
Synthetic peptide - Human
Dilution info
-
Notes

-

Tested
Tested

Species
Synthetic peptide - Human
Dilution info
1/1000
Notes

-

Expected
Expected

Species
Human
Dilution info
Use at an assay dependent concentration.
Notes

-

Tested
Tested

Species
Human
Dilution info
1/1000
Notes

-

Not recommended
Not recommended

Species
Synthetic peptide - Human
Dilution info
-
Notes

-

Associated Products

Select an associated product type

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Target data

Function

The protein expressed by the gene PDCD1 functions as an inhibitory receptor on antigen-activated T-cells, playing a critical role in the induction and maintenance of immune tolerance to self. It delivers inhibitory signals by binding to the ligands CD274/PDCD1L1 and CD273/PDCD1LG2. Tumors exploit the PDCD1-mediated inhibitory pathway to attenuate anti-tumor immunity, enabling tumor survival by evading immune system destruction. The interaction with CD274/PDCD1L1 inhibits the effector function of cytotoxic T lymphocytes (CTLs). Blocking the PDCD1-mediated pathway can reverse the exhausted T-cell phenotype and normalize the anti-tumor response, providing a rationale for cancer immunotherapy. This supplementary information is collated from multiple sources and compiled automatically.

Alternative names

CD279, PD1, PDCD1, Programmed cell death protein 1, Protein PD-1, hPD-1

Recommended products

Anti-PD1 (phospho Y248) antibody [EPR19821] (ab206378) is a rabbit monoclonal antibody detecting PD1 in Western Blot, IP, Dot Blot. Suitable for Human.



- Biophysical QC for unrivalled batch-batch consistency

Key facts

Isotype
IgG
Form
Liquid
Clonality
Monoclonal
Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Clone number
EPR19821
Purification technique
Affinity purification Protein A
Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle

Notes

What is this antibody validated in?


Anti-PD1 (phospho Y248) antibody [EPR19821] (ab206378) is a rabbit recombinant monoclonal antibody and is validated for use in Western Blot (WB), Immunoprecipitation (IP), Dot Blot in Human samples.

What is the molecular weight of PD1?


Anti-PD1 (phospho Y248) [EPR19821] (ab206378) specifically detects a band for PD1 (UniProt: Q15116) at a molecular weight of 32kDa.

Trial sizes available!


Test your antibody or perform pre-screening before committing to a larger quantity. Sold in 10µl. Discover our selection of trial-size antibodies.

Other related products


We have a range of other formats of antibody clone [EPR19821] also available for your convenience:
ab206378, Carrier free - Anti-PD1 (phospho Y248) antibody [EPR19821] - BSA and Azide free ab234960



Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.
Activity summary

PD1 also known as Programmed Cell Death Protein 1 or PDCD1 is a transmembrane protein that plays a critical role in regulating immune responses. It has a mass of approximately 55 kDa. PD1 is expressed on the surface of T cells B cells and some myeloid cells. PD1’s expression increases upon activation of these immune cells assisting in maintaining peripheral tolerance. Researchers often use PD1 mouse models and chimeric antibodies to explore the function of PD1 for experimental purposes. Antibodies such as anti-PD1 such as EH12.2H7 help in blocking PD1 interaction to study its role further.

Biological function summary

PD1 serves as an inhibitory receptor acting as a checkpoint in the immune system. It becomes part of an immune-suppressive complex when it binds with its ligands PD-L1 or PD-L2 which are expressed on various cell types including some tumor cells. This interaction suppresses the proliferation of T cells and cytokine production contributing to immune homeostasis. By controlling T cell activity PD1 limits autoimmunity but can also reduce the immune system's capability to attack cancer cells.

Pathways

PD1 functions in the immune checkpoint pathway a critical regulatory circuit in immune regulation. The engagement of PD1 with its ligands initiates a cascade that inhibits the function and proliferation of T cells through downstream SHP-2 phosphatase activity. This pathway frequently involves other regulatory proteins like CTLA-4 and is an important mechanism by which the body modulates immune responses. Related pathways often intersect with those involving T cell receptor signaling and contribute to the overall modulation of immune activity.

Associated diseases and disorders

PD1 has a significant role in cancer and autoimmune disorders. PD1 expression can allow tumors to evade immune surveillance making PD1 a target for cancer therapies such as anti-PD1 antibodies which aim to block PD1 and restore T cell activity. The interaction of PD1 with cancer-related proteins like PD-L1 facilitates tumor immune evasion. In autoimmune disorders PD1’s regulation of immune balance can become dysregulated leading to persistent immune activation and tissue damage. Understanding PD1 and its interaction with proteins such as PD-L1 helps in developing therapeutic strategies for both cancer and autoimmune conditions.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

3 product images

  • Immunoprecipitation - Anti-PD1 (phospho Y248) antibody [EPR19821] (ab206378), expandable thumbnail

    Immunoprecipitation - Anti-PD1 (phospho Y248) antibody [EPR19821] (ab206378)

    PD1 (phospho Y248) was immunoprecipitated from 0.35 mg of HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) transfected with a PD1 (WT) plus 25 kDa fusion protein expression vector, then treated with 100 μM pervanadate for 10 minutes whole cell lysate, with ab206378 at 1/30 dilution. Western blot was performed from the immunoprecipitate using ab206378 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (VeriBlot for IP Detection Reagent (HRP) ab131366) was used for detection at 1/5000 dilution.

    Lane 1: HEK-293T transfected with a PD1 (WT) plus 25 kDa fusion protein expression vector, then treated with 100 μM pervanadate for 10 minutes whole cell lysate 10 μg (Input).

    Lane 2: ab206378 IP in HEK-293T transfected with a PD1 (WT) plus 25 kDa fusion protein expression vector, then treated with 100 μM pervanadate for 10 minutes whole cell lysate.

    Lane 3: Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) instead of ab206378 in HEK-293T transfected with a PD1 (WT) plus 25 kDa fusion protein expression vector, then treated with 100 μM pervanadate for 10 minutes whole cell lysate.

    Exposure time: 30 seconds
    Blocking and diluting buffer and concentration: 5% NFDM/TBST

    The observed bands around 75 kDa comprise PD-1 plus the 25 kDa fusion protein. The plasmids were kindly provided by our collaborator Dr. Liang Chen, NIBS.

    All lanes: Immunoprecipitation - Anti-PD1 (phospho Y248) antibody [EPR19821] (ab206378)

    Predicted band size: 32 kDa

    Observed band size: 70-75 kDa

  • Western blot - Anti-PD1 (phospho Y248) antibody [EPR19821] (ab206378), expandable thumbnail

    Western blot - Anti-PD1 (phospho Y248) antibody [EPR19821] (ab206378)

    Blocking/Dilution buffer: 5% NFDM/TBST.

    The observed bands at around 75 kDa are PD-1 plus the 25 kDa fusion protein. The expression profile observed is consistent with what has been described in the literature (PMID: 22641383). The plasmids were kindly provided by our collaborator Dr. Liang Chen NIBS.

    All lanes: Western blot - Anti-PD1 (phospho Y248) antibody [EPR19821] (ab206378) at 1/1000 dilution

    Lane 1: Untreated-HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate transfected with a PD1 (WT) plus a 25 kDa fusion protein expression vector at 20 µg

    Lane 2: HEK-293T transfected with a PD1 (WT) plus a 25 kDa fusion protein expression vector, then treated with 100 μM pervanadate for 10 minutes whole cell lysate at 20 µg

    Lane 3: HEK-293T transfected with PD1 (WT) plus a 25 kDa fusion protein expression vector, then treated with 100 μM pervanadate for 10 minutes, whole cell lysate (20 μg). Following the transfer to PVDF, the membrane was treated with alkaline phosphatase for 1h at 37°C. at 20 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution

    Predicted band size: 32 kDa

    Observed band size: 70-75 kDa

    Exposure time: 30s

  • Dot Blot - Anti-PD1 (phospho Y248) antibody [EPR19821] (ab206378), expandable thumbnail

    Dot Blot - Anti-PD1 (phospho Y248) antibody [EPR19821] (ab206378)

    Dot blot analysis of PD1 (phospho Y248) labeled with ab206378 at 1/1000 dilution.

    Lane 1: PD1 (phospho Y248) peptide.
    Lane 2: PD1 non-phospho peptide.

    Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution was used as secondary antibody.

    Blocking/Dilution buffer: 5% NFDM/TBST.

    Exposure time: 1 minute.

    The blot was developed on a BIO-RAD® ChemiDoc™ MP instrument.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

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