Anti-PDCD2 antibody [EPR7158]

• WB

Lab

Western blot - Anti-PDCD2 antibody [EPR7158] (AB133324)

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

All lanes:

Western blot - Anti-PDCD2 antibody [EPR7158] (ab133324) at 1/1000 dilution

Lane 1:

293T (human embryonic kidney epithelial cell) whole cell lysate at 20 µg

Lane 2:

HeLa (human cervix adenocarcinoma epithelial cell) whole cell lysate at 20 µg

Lane 3:

Ramos (human Burkitt's lymphoma B lymphocyte) whole cell lysate at 20 µg

Lane 4:

NIH/3T3 (mouse embryonic fibroblast) whole cell lysate at 20 µg

Lane 5:

RAW264.7 (mouse Abelson murine leukemia virus-induced tumor macrophage) whole cell lysate at 20 µg

Lane 6:

C2C12 (mouse myoblasts myoblast) whole cell lysate at 20 µg

Lane 7:

C6 (rat glial tumor glial cell) whole cell lysate at 20 µg

Lane 8:

PC-12 (rat adrenal gland pheochromocytoma) whole cell lysate at 20 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution

false

Exposure time: 10s

• WB

Lab

Western blot - Anti-PDCD2 antibody [EPR7158] (AB133324)

ab126614 shows low sensitivity; therefore, we recommend ab133324 as an alternative.

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

Lanes 1 - 3:

Western blot - Anti-PDCD2 antibody [EPR7159] (<a href='/en-us/products/primary-antibodies/pdcd2-antibody-epr7159-ab126614'>ab126614</a>) at 1/1000 dilution

Lanes 4 - 6:

Western blot - Anti-PDCD2 antibody [EPR7158] (ab133324) at 1/1000 dilution

Lanes 1 and 4:

293T (human embryonic kidney epithelial cell) whole cell lysate at 20 µg

Lanes 2 and 5:

HeLa (human cervix adenocarcinoma epithelial cell) whole cell lysate at 20 µg

Lanes 3 and 6:

Ramos (human Burkitt's lymphoma B lymphocyte) whole cell lysate at 20 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution

Observed band size: 39 kDa

false

Exposure time: 20s

• WB

Lab

Western blot - Anti-PDCD2 antibody [EPR7158] (AB133324)

This antibody detects a non-specific protein close to PDCD2 in tissue samples.

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

All lanes:

Western blot - Anti-PDCD2 antibody [EPR7158] (ab133324) at 1/1000 dilution

Lane 1:

Human heart tissue lysate at 20 µg

Lane 2:

Human kidney tissue lysate at 20 µg

Lane 3:

Human brain tissue lysate at 20 µg

Lane 4:

Mouse heart tissue lysate at 20 µg

Lane 5:

Mouse kidney tissue lysate at 20 µg

Lane 6:

Mouse brain tissue lysate at 20 µg

Lane 7:

Rat heart tissue lysate at 20 µg

Lane 8:

Rat kidney tissue lysate at 20 µg

Lane 9:

Rat brain at 20 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution

false

Exposure time: 180s

• OI-RD Scanning

Unknown

OI-RD Scanning - Anti-PDCD2 antibody [EPR7158] (AB133324)

We have systematically measured KD (the equilibrium dissociation constant between the antibody and its antigen), of more than 840 recombinant antibodies to assess not only their individual KD values but also to see the average affinity of antibody. Based on the comparison with published literature values for mouse monoclonal antibodies, Recombinant antibodies appear to be on average 1-2 order of magnitude higher affinity.