JavaScript is disabled in your browser. Please enable JavaScript to view this website.
AB327166

Anti-PDCD4 Antibody

Be the first to review this product! Submit a review

|

(0 Publication)

Rabbit Polyclonal PDCD4 antibody. Suitable for WB, IHC-P, ChIP and reacts with Rat, Human, Mouse samples. Immunogen corresponding to Recombinant Fragment Protein within Human PDCD4.

View Alternative Names

H731, PDCD4, Programmed cell death protein 4, Neoplastic transformation inhibitor protein, Nuclear antigen H731-like, Protein 197/15a, Ma3, Tis, Programmed cell death protein 4, Protein MA-3, Topoisomerase-inhibitor suppressed protein, Dug, Programmed cell death protein 4, Death up-regulated gene protein

9 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PDCD4 Antibody (AB327166)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PDCD4 Antibody (AB327166)

Immunohistochemistry analysis in human breast and skeletal muscle tissues using ab327166 at 1 : 200–1 : 500 dilution. Corresponding PDCD4 RNA-seq data are presented for the same tissues.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PDCD4 Antibody (AB327166)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PDCD4 Antibody (AB327166)

Immunohistochemical staining of human thyroid gland using ab327166 at 1 : 200–1 : 500 dilution, showing strong nuclear positivity in glandular cells.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PDCD4 Antibody (AB327166)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PDCD4 Antibody (AB327166)

Immunohistochemical staining of human skin using ab327166 at 1 : 200–1 : 500 dilution, showing strong nuclear positivity in epidermal cells.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PDCD4 Antibody (AB327166)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PDCD4 Antibody (AB327166)

Immunohistochemical staining of human small intestine using ab327166 at 1 : 200–1 : 500 dilution, showing strong nuclear positivity in glandular cells.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PDCD4 Antibody (AB327166)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PDCD4 Antibody (AB327166)

Immunohistochemical staining of human breast using ab327166 at 1 : 200–1 : 500 dilution, showing strong nuclear positivity in glandular cells.

ChIP - Anti-PDCD4 Antibody (AB327166)
  • ChIP

Supplier Data

ChIP - Anti-PDCD4 Antibody (AB327166)

ChIP-Exo-Seq composite graph for ab327166 tested in K562 cells at 1–10 µg per reaction. Strand-specific reads (blue : forward, red : reverse) and IgG controls (black : forward, grey : reverse) are plotted against the distance from a composite set of reference binding sites. The antibody exhibits robust target enrichment compared to a non-specific IgG control and precisely reveals its structural organization around the binding site. Data generated by Prof. B. F. Pugh´s Lab at Cornell University.

Western blot - Anti-PDCD4 Antibody (AB327166)
  • WB

Supplier Data

Western blot - Anti-PDCD4 Antibody (AB327166)

Western blot analysis in human cell line Daudi using ab327166 at 0.04–0.4 µg/ml.

All lanes:

Western blot - Anti-PDCD4 Antibody (ab327166)

All lanes:

Human cell line Daudi

Predicted band size: 51.74 kDa

false

Western blot - Anti-PDCD4 Antibody (AB327166)
  • WB

Supplier Data

Western blot - Anti-PDCD4 Antibody (AB327166)

Western blot analysis in mouse cell line NIH-3T3, rat cell line NBT-II and rat cell line pC12 using ab327166 at 0.04–0.4 µg/ml.

All lanes:

Western blot - Anti-PDCD4 Antibody (ab327166)

Lane 2:

Mouse cell line NIH-3T3

Lane 3:

Rat cell line NBT-II

Lane 4:

Rat cell line pC12

Predicted band size: 51.74 kDa

false

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PDCD4 Antibody (AB327166)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PDCD4 Antibody (AB327166)

Immunohistochemical staining of human skeletal muscle using ab327166 at 1 : 200–1 : 500 dilution, showing no positivity in myocytes as expected.

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human, Mouse, Rat

Applications

ChIP, WB, IHC-P

applications

Immunogen

Recombinant Fragment Protein within Human PDCD4.

Q53EL6

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ChIP" : {"fullname" : "ChIP", "shortname":"ChIP"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.04-0.4 µg/mL", "WB-species-notes": "<p></p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/200 - 1/500", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval before commencing with IHC staining protocol.", "ChIP-species-checked": "testedAndGuaranteed", "ChIP-species-dilution-info": "1-10 µg", "ChIP-species-notes": "<p></p>" }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.04-0.4 µg/mL", "WB-species-notes": "<p></p>", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ChIP-species-checked": "guaranteed", "ChIP-species-dilution-info": "", "ChIP-species-notes": "" }, "Rat": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.04-0.4 µg/mL", "WB-species-notes": "<p></p>", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ChIP-species-checked": "guaranteed", "ChIP-species-dilution-info": "", "ChIP-species-notes": "" } } }

Properties and storage information

Form
Liquid
Purification technique
Affinity purification
Storage buffer
pH: 7.2 Preservative: 0.02% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine)
Shipped at conditions
Ambient - Can Ship with Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Target data

Inhibits translation initiation and cap-dependent translation. May excert its function by hindering the interaction between EIF4A1 and EIF4G. Inhibits the helicase activity of EIF4A. Modulates the activation of JUN kinase. Down-regulates the expression of MAP4K1, thus inhibiting events important in driving invasion, namely, MAPK85 activation and consequent JUN-dependent transcription. May play a role in apoptosis. Tumor suppressor. Inhibits tumor promoter-induced neoplastic transformation. Binds RNA (By similarity).
See full target information PDCD4

Additional targets

,

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com