AB51495

Anti-PDCD4 antibody

4

(6 Reviews)

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(43 Publications)

Rabbit Polyclonal PDCD4 antibody. Suitable for WB, IHC-P and reacts with Human, Recombinant full length protein, Mouse samples. Cited in 43 publications. Immunogen corresponding to Synthetic Peptide within Human PDCD4.

View Alternative Names

H731, PDCD4, Programmed cell death protein 4, Neoplastic transformation inhibitor protein, Nuclear antigen H731-like, Protein 197/15a

3 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PDCD4 antibody (AB51495)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PDCD4 antibody (AB51495)

ab51495, at 1/100 dilution, staining human PDCD4 in paraformaldehyde fixed (4%), paraffin embedded mouse colon tissue by Immunohistochemistry.

Western blot - Anti-PDCD4 antibody (AB51495)

WB

AbReview30853****

Western blot - Anti-PDCD4 antibody (AB51495)

13% SDS-Page gel used, Blocking done for 1 hour at RT, Primary Incubation overnight at 4°C.

All lanes:

Western blot - Anti-PDCD4 antibody (ab51495) at 1/1000 dilution

Lane 1:

Human HEK cell Lysate at 30 µg/mL

Lane 2:

Mouse N2A cell Lysate at 30 ug/ml at 30 µg/mL

Secondary

All lanes:

Li-Cor IRDye<sup>®</sup>800CW-conjugated Goat anti rabbit

Predicted band size: 51 kDa

false

This image is courtesy of an anonymous Abreview

Western blot - Anti-PDCD4 antibody (AB51495)

WB

Unknown

Western blot - Anti-PDCD4 antibody (AB51495)

All lanes:

Western blot - Anti-PDCD4 antibody (ab51495) at 1/5000 dilution

Lane 1:

recombinant PDCD4

Lane 2:

293 HEK cells treated with TPA and MG132.

Predicted band size: 51 kDa

Observed band size: 52 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Mouse, Human

Applications

IHC-P, WB

Immunogen

Synthetic Peptide within Human PDCD4. The exact immunogen used to generate this antibody is proprietary information.

Specificity

ab51495 reacts with PDCD4 protein that is either phosphorylated or non phosphorylated at Ser 457.

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PDCD4 antibody [EPR3432] (AB79405)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/300", "IHCP-species-notes": "<p></p>" }, "Rat": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Recombinant full length protein": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Xenopus laevis": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Purification notes

ab51495 was affinity purified from monospecific antiserum by immunoaffinity chromatography.

Storage buffer

pH: 7.2 Preservative: 0.01% Sodium azide Constituents: 0.88% Sodium chloride, 0.424% Potassium phosphate solution

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The PDCD4 protein also known as Programmed Cell Death 4 is a multifunctional tumor suppressor. With a molecular mass of approximately 64 kDa it plays an important role in regulating gene expression and inhibiting cellular transformation. PDCD4 is expressed in various tissues including the lung kidney and heart where it modulates different cellular pathways. Its ability to bind RNA and interact with eukaryotic initiation factor 4A (eIF4A) highlights its important role in translation regulation.

Biological function summary

PDCD4 inhibits translation by binding to the eIF4A RNA helicase blocking the translation initiation complex. This action stabilizes mRNA and reduces protein synthesis impacting processes such as cell growth and proliferation. Although PDCD4 functions mainly as a monomer it can also interact with other proteins to form complexes that enhance its regulatory activity. More specifically its role in controlling AP-1 transcription factor activity steps into the spotlight in influencing cellular responses.

Pathways

PDCD4 primarily interacts with signaling mechanisms related to apoptosis and inflammation. The protein is integral in the mTOR signaling pathway which governs cell growth in response to nutrient availability. PDCD4 also impacts the PI3K/AKT pathway linking its activity to cellular survival and metabolism. Through these pathways PDCD4 associates with proteins such as p53 and NF-kB connecting it to broader cellular and molecular networks involved in maintaining cellular integrity.

PDCD4 is significantly implicated in cancer progression and inflammation-related conditions. Its downregulation or loss frequently occurs in several cancers including breast and lung cancer leading to unchecked cell proliferation and tumor growth. PDCD4's interaction with proteins like c-Myc further demonstrates its importance in oncogenic pathways. Additionally its role in inflammation positions it as a critical factor in disorders such as Crohn’s disease where abnormal inflammatory responses are prominent.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Inhibits translation initiation and cap-dependent translation. May excert its function by hindering the interaction between EIF4A1 and EIF4G. Inhibits the helicase activity of EIF4A. Modulates the activation of JUN kinase. Down-regulates the expression of MAP4K1, thus inhibiting events important in driving invasion, namely, MAPK85 activation and consequent JUN-dependent transcription. May play a role in apoptosis. Tumor suppressor. Inhibits tumor promoter-induced neoplastic transformation. Binds RNA (By similarity).

See full target information PDCD4

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Publications (43)

Recent publications for all applications. Explore the full list and refine your search

Scientific reports 15:24100 PubMed40619518

2025

Rosmarinic acid potentiates gefitinib in lung adenocarcinoma by modulating interactions between cancer cells and cancer-associated fibroblasts.

Applications

Unspecified application

Species

Unspecified reactive species

Duo Li,Yiying Lv,Leihao Hu,Anqi Sun,Lingling Sun

Theranostics 14:3777-3792 PubMed38994018

2024

Targeted delivery of anti-miRNA21 sensitizes PD-L1 tumor to immunotherapy by promoting immunogenic cell death.

Applications

Unspecified application

Species

Unspecified reactive species

Eun Hye Kim,Jiwoong Choi,Hochung Jang,Yelee Kim,Jong Won Lee,Youngri Ryu,Jiwon Choi,Yeonho Choi,Sung-Gil Chi,Ick Chan Kwon,Yoosoo Yang,Sun Hwa Kim

Cytotherapy 25:625-639 PubMed36868991

2023

Bone mesenchymal stromal cell-derived small extracellular vesicles inhibit inflammation and ameliorate sepsis via delivery of microRNA-21a-5p.

Applications

Unspecified application

Species

Unspecified reactive species

Ruichao Niu,Pinhua Pan,Chonghui Li,Baihua Luo,Hua Ma,Haojie Hao,Zhigang Zhao,Hang Yang,Shiyang Ma,Fei Zhu,Jie Chen

Acta biochimica et biophysica Sinica 54:1057-1067 PubMed35983977

2022

WWP2 ameliorates oxidative stress and inflammation in atherosclerotic mice through regulation of PDCD4/HO-1 pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Xingye Wang,Lu Ma,Songlin Zhang,Qiang Song,Xumei He,Jun Wang

Oxidative medicine and cellular longevity 2022:5938688 PubMed35295718

2022

Anti-PD-L1 Antibody Enhances T Cell Immune Responses and Reduces Resistance of Breast Cancer Cells to Radiotherapy.

Applications

Unspecified application

Species

Unspecified reactive species

Lei-Ming Guo,Gao-Feng Ding,Wen-Cai Xu,Hong Ge,Yue Jiang,Yu-Fei Lu

Aging 13:25484-25495 PubMed34905503

2021

Hsa_circ_0008259 modulates miR-21-5p and PDCD4 expression to restrain osteosarcoma progression.

Applications

Unspecified application

Species

Unspecified reactive species

Kai Guan,Shizhang Liu,Keke Duan,Xiaoxia Zhang,Huitong Liu,Bingqiang Xu,Xi Wang,Xin Jin

Journal of occupational health 63:e12282 PubMed34679210

2021

MicroRNA expression in lung tissues of asbestos-exposed mice: Upregulation of miR-21 and downregulation of tumor suppressor genes Pdcd4 and Reck.

Applications

Unspecified application

Species

Unspecified reactive species

Yusuke Hiraku,Jun Watanabe,Akira Kaneko,Takamichi Ichinose,Mariko Murata

Frontiers in oncology 11:699475 PubMed34458143

2021

Screening of MicroRNA Related to Irradiation Response and the Regulation Mechanism of miRNA-96-5p in Rectal Cancer Cells.

Applications

Unspecified application

Species

Unspecified reactive species

Fengpeng Wu,Bingyue Wu,Xiaoxiao Zhang,Congrong Yang,Chaoxi Zhou,Shuguang Ren,Jun Wang,Yafan Yang,Guiying Wang

Acta diabetologica 58:1251-1267 PubMed33907874

2021

Long non-coding RNA KCNQ1OT1 increases the expression of PDCD4 by targeting miR-181a-5p, contributing to cardiomyocyte apoptosis in diabetic cardiomyopathy.

Applications

Unspecified application

Species

Unspecified reactive species

Shuo-Fang Zhao,Ying-Xian Ye,Jin-Dong Xu,Yi He,Deng-Wen Zhang,Zheng-Yuan Xia,Sheng Wang

BMC cancer 21:284 PubMed33726686

2021

Circ_0003266 sponges miR-503-5p to suppress colorectal cancer progression via regulating PDCD4 expression.

Applications

Unspecified application

Species

Unspecified reactive species

Caihong Wen,Xiaoqing Feng,Honggang Yuan,Yong Gong,Guangsheng Wang

View all publications

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Product promise

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