AB105998

Anti-PDCD4 (phospho S457) antibody [9G6]

Name: Anti-PDCD4 (phospho S457) antibody [9G6] (ab105998) | Abcam
Brand: Abcam Limited
SKU: AB105998
Price: 500 USD
Availability: InStock

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(3 Publications)

Mouse Monoclonal PDCD4 phospho S457 antibody. Suitable for WB, IHC-P and reacts with Mouse, Human samples. Cited in 3 publications. Immunogen corresponding to Synthetic Peptide within Human PDCD4 pS457.

View Alternative Names

H731, PDCD4, Programmed cell death protein 4, Neoplastic transformation inhibitor protein, Nuclear antigen H731-like, Protein 197/15a

2 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PDCD4 (phospho S457) antibody [9G6] (AB105998)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PDCD4 (phospho S457) antibody [9G6] (AB105998)

ab105998 at 1/1000 dilution staining PDCD4 in Human small intestine by Immunohistochemistry Formalin-fixed, Paraffin-embedded tissue.
The image shows the localization of ab105998 as the precipitated red signal, with a hematoxylin purple nuclear counterstain.

Unknown

Western blot - Anti-PDCD4 (phospho S457) antibody [9G6] (AB105998)

All lanes:

Western blot - Anti-PDCD4 (phospho S457) antibody [9G6] (ab105998) at 1/2000 dilution

Lane 1:

Unstimulated (non-phosphorylated) NIH 3T3 cells

Lane 2:

NIH-3T3 cells stimulated with 30 ng/ml PDGF (5 min treatment)

Secondary

All lanes:

HRP conjugated Rabbit anti-Mouse IgG at 1/20000 dilution

Predicted band size: 51 kDa

false

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

9G6

Isotype

IgG1

Carrier free

Reacts with

Mouse, Human

Applications

IHC-P, WB

applications

Immunogen

Synthetic Peptide within Human PDCD4 pS457. The exact immunogen used to generate this antibody is proprietary information.

Q53EL6

Specificity

This antibody is specific for human Pdcd4 protein phosphorylated at Ser457. A BLAST analysis was used to suggest cross-reactivity with Pdcd4 from human, mouse, rat and Xenopus based on 100% homology with the immunizing sequence. Cross-reactivity with Pdcd4 from other sources has not been determined.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/1000 - 1/5000", "IHCP-species-notes": "" }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/2000 - 1/10000", "WB-species-notes": "", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Rat": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Xenopus laevis": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Purification notes

Purified from concentrated tissue culture supernate by Protein A chromatography.

Storage buffer

pH: 7.2 Preservative: 0.01% Sodium azide Constituents: 0.88% Sodium chloride, 0.424% Potassium phosphate solution

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The PDCD4 protein also known as Programmed Cell Death 4 is a multifunctional tumor suppressor. With a molecular mass of approximately 64 kDa it plays an important role in regulating gene expression and inhibiting cellular transformation. PDCD4 is expressed in various tissues including the lung kidney and heart where it modulates different cellular pathways. Its ability to bind RNA and interact with eukaryotic initiation factor 4A (eIF4A) highlights its important role in translation regulation.

Biological function summary

PDCD4 inhibits translation by binding to the eIF4A RNA helicase blocking the translation initiation complex. This action stabilizes mRNA and reduces protein synthesis impacting processes such as cell growth and proliferation. Although PDCD4 functions mainly as a monomer it can also interact with other proteins to form complexes that enhance its regulatory activity. More specifically its role in controlling AP-1 transcription factor activity steps into the spotlight in influencing cellular responses.

Pathways

PDCD4 primarily interacts with signaling mechanisms related to apoptosis and inflammation. The protein is integral in the mTOR signaling pathway which governs cell growth in response to nutrient availability. PDCD4 also impacts the PI3K/AKT pathway linking its activity to cellular survival and metabolism. Through these pathways PDCD4 associates with proteins such as p53 and NF-kB connecting it to broader cellular and molecular networks involved in maintaining cellular integrity.

PDCD4 is significantly implicated in cancer progression and inflammation-related conditions. Its downregulation or loss frequently occurs in several cancers including breast and lung cancer leading to unchecked cell proliferation and tumor growth. PDCD4's interaction with proteins like c-Myc further demonstrates its importance in oncogenic pathways. Additionally its role in inflammation positions it as a critical factor in disorders such as Crohn’s disease where abnormal inflammatory responses are prominent.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Inhibits translation initiation and cap-dependent translation. May excert its function by hindering the interaction between EIF4A1 and EIF4G. Inhibits the helicase activity of EIF4A. Modulates the activation of JUN kinase. Down-regulates the expression of MAP4K1, thus inhibiting events important in driving invasion, namely, MAPK85 activation and consequent JUN-dependent transcription. May play a role in apoptosis. Tumor suppressor. Inhibits tumor promoter-induced neoplastic transformation. Binds RNA (By similarity).

See full target information PDCD4 pS457

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Publications (3)

Recent publications for all applications. Explore the full list and refine your search

Pigment cell & melanoma research 32:564-575 PubMed30672666

2019

Conjunctival melanoma copy number alterations and correlation with mutation status, tumor features, and clinical outcome.

Applications

Unspecified application

Species

Unspecified reactive species

Nihal Kenawy,Helen Kalirai,Joseph J Sacco,Sarah L Lake,Steffen Heegaard,Ann-Cathrine Larsen,Paul T Finger,Tatyana Milman,Kimberly Chin,Carlo Mosci,Francesco Lanza,Alexandre Moulin,Caroline A Schmitt,Jean Pierre Caujolle,Célia Maschi,Marina Marinkovic,Azzam F Taktak,Heinrich Heimann,Bertil E Damato,Sarah E Coupland

Oncology letters 14:3371-3378 PubMed28927090

2017

Downregulation of PDCD4 by miR-21 suppresses tumor transformation and proliferation in a nude mouse renal cancer model.

Applications

Unspecified application

Species

Unspecified reactive species

Haixin Yuan,Shiyong Xin,Yaoping Huang,Yingfan Bao,Hao Jiang,Liqing Zhou,Xiaoqiang Ren,Liang Li,Qian Wang,Jianguo Zhang

Molecular carcinogenesis 56:886-894 PubMed27533779

2016

MicroRNA-21 regulates the ERK/NF-κB signaling pathway to affect the proliferation, migration, and apoptosis of human melanoma A375 cells by targeting SPRY1, PDCD4, and PTEN.

Applications

Unspecified application

Species

Unspecified reactive species

Xu-Hua Mao,Min Chen,Yan Wang,Pan-Gen Cui,Si-Bian Liu,Zei-Yong Xu

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-PDCD4 (phospho S457) antibody [9G6]

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PDCD4 (phospho S457) antibody [9G6] (AB105998)

Western blot - Anti-PDCD4 (phospho S457) antibody [9G6] (AB105998)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Specificity

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Reactivity data

Properties and storage information

Form

Purification technique

Purification notes

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (3)

Product promise