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AB73343

Anti-PDCD4 (phospho S67) antibody

0

(1 Review)

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(8 Publications)

Rabbit Polyclonal PDCD4 phospho S67 antibody. Suitable for WB and reacts with Human samples. Cited in 8 publications. Immunogen corresponding to Synthetic Peptide within Human PDCD4 phospho S67.

View Alternative Names

H731, PDCD4, Programmed cell death protein 4, Neoplastic transformation inhibitor protein, Nuclear antigen H731-like, Protein 197/15a

1 Images
Western blot - Anti-PDCD4 (phospho S67) antibody (AB73343)
  • WB

Unknown

Western blot - Anti-PDCD4 (phospho S67) antibody (AB73343)

All lanes:

Western blot - Anti-PDCD4 (phospho S67) antibody (ab73343) at 1/500 dilution

Lane 1:

HepG2 cell extract at 30 µg

Lane 2:

HepG2 cell extract at 30 µg with immunising phosphopeptide

Predicted band size: 51 kDa

Observed band size: 52 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

WB

applications

Immunogen

Synthetic Peptide within Human PDCD4 phospho S67. The exact immunogen used to generate this antibody is proprietary information.

Q53EL6

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Reactivity data

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Purification notes
ab73343 was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
Storage buffer
pH: 7.4 Preservative: 0.02% Sodium azide Constituents: PBS, 50% Glycerol (glycerin, glycerine), 0.87% Sodium chloride
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Storage information
Stable for 12 months at -20°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The PDCD4 protein also known as Programmed Cell Death 4 is a multifunctional tumor suppressor. With a molecular mass of approximately 64 kDa it plays an important role in regulating gene expression and inhibiting cellular transformation. PDCD4 is expressed in various tissues including the lung kidney and heart where it modulates different cellular pathways. Its ability to bind RNA and interact with eukaryotic initiation factor 4A (eIF4A) highlights its important role in translation regulation.
Biological function summary

PDCD4 inhibits translation by binding to the eIF4A RNA helicase blocking the translation initiation complex. This action stabilizes mRNA and reduces protein synthesis impacting processes such as cell growth and proliferation. Although PDCD4 functions mainly as a monomer it can also interact with other proteins to form complexes that enhance its regulatory activity. More specifically its role in controlling AP-1 transcription factor activity steps into the spotlight in influencing cellular responses.

Pathways

PDCD4 primarily interacts with signaling mechanisms related to apoptosis and inflammation. The protein is integral in the mTOR signaling pathway which governs cell growth in response to nutrient availability. PDCD4 also impacts the PI3K/AKT pathway linking its activity to cellular survival and metabolism. Through these pathways PDCD4 associates with proteins such as p53 and NF-kB connecting it to broader cellular and molecular networks involved in maintaining cellular integrity.

PDCD4 is significantly implicated in cancer progression and inflammation-related conditions. Its downregulation or loss frequently occurs in several cancers including breast and lung cancer leading to unchecked cell proliferation and tumor growth. PDCD4's interaction with proteins like c-Myc further demonstrates its importance in oncogenic pathways. Additionally its role in inflammation positions it as a critical factor in disorders such as Crohn’s disease where abnormal inflammatory responses are prominent.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Inhibits translation initiation and cap-dependent translation. May excert its function by hindering the interaction between EIF4A1 and EIF4G. Inhibits the helicase activity of EIF4A. Modulates the activation of JUN kinase. Down-regulates the expression of MAP4K1, thus inhibiting events important in driving invasion, namely, MAPK85 activation and consequent JUN-dependent transcription. May play a role in apoptosis. Tumor suppressor. Inhibits tumor promoter-induced neoplastic transformation. Binds RNA (By similarity).
See full target information PDCD4 phospho S67
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Publications (8)

Recent publications for all applications. Explore the full list and refine your search

Communications biology 6:1252 PubMed38081915

2023

Atg7 senses ATP levels and regulates AKT-PDCD4 phosphorylation-ubiquitination axis to promote survival during metabolic stress.

Applications

Unspecified application

Species

Unspecified reactive species

Chengsi Deng,Chunlu Li,Xiang Dong,Yang Yu,Wendong Guo,Yi Guan,Xun Sun,Liu Cao

Nature communications 13:2904 PubMed35614056

2022

O-GlcNAc modification of leucyl-tRNA synthetase 1 integrates leucine and glucose availability to regulate mTORC1 and the metabolic fate of leucine.

Applications

Unspecified application

Species

Unspecified reactive species

Kibum Kim,Hee Chan Yoo,Byung Gyu Kim,Sulhee Kim,Yulseung Sung,Ina Yoon,Ya Chun Yu,Seung Joon Park,Jong Hyun Kim,Kyungjae Myung,Kwang Yeon Hwang,Sunghoon Kim,Jung Min Han

ACS chemical biology 16:2808-2815 PubMed34780684

2021

Modulation of Phosphoprotein Activity by Phosphorylation Targeting Chimeras (PhosTACs).

Applications

Unspecified application

Species

Unspecified reactive species

Po-Han Chen,Zhenyi Hu,Elvira An,Ifunanya Okeke,Sijin Zheng,Xuanmeng Luo,Angela Gong,Saul Jaime-Figueroa,Craig M Crews

Journal of experimental & clinical cancer research 38:76 PubMed30760284

2019

SKP2 promotes breast cancer tumorigenesis and radiation tolerance through PDCD4 ubiquitination.

Applications

Unspecified application

Species

Unspecified reactive species

Ce Li,Lutao Du,Yidan Ren,Xiaoyan Liu,Qinlian Jiao,Donghai Cui,Mingxin Wen,Chuanxin Wang,Guangwei Wei,Yunshan Wang,Aiguo Ji,Qin Wang

The Journal of biological chemistry 291:2389-96 PubMed26645692

2015

Interferon γ (IFNγ) Signaling via Mechanistic Target of Rapamycin Complex 2 (mTORC2) and Regulatory Effects in the Generation of Type II Interferon Biological Responses.

Applications

Unspecified application

Species

Unspecified reactive species

Barbara Kroczynska,Robert L Rafidi,Beata Majchrzak-Kita,Ewa M Kosciuczuk,Gavin T Blyth,Jacek Jemielity,Zofia Warminska,Diana Saleiro,Swarna Mehrotra,Ahmet Dirim Arslan,Eleanor N Fish,Leonidas C Platanias

PloS one 9:e91564 PubMed24626527

2014

Atheroprotective pulsatile flow induces ubiquitin-proteasome-mediated degradation of programmed cell death 4 in endothelial cells.

Applications

Unspecified application

Species

Unspecified reactive species

Cheng Ge,Jiantao Song,Liang Chen,Lin Wang,Yifei Chen,Xinxin Liu,Yu Zhang,Lining Zhang,Mei Zhang

Proceedings of the National Academy of Sciences of the United States of America 108:14632-6 PubMed21844356

2011

US3 protein kinase of HSV-1 cycles between the cytoplasm and nucleus and interacts with programmed cell death protein 4 (PDCD4) to block apoptosis.

Applications

Unspecified application

Species

Unspecified reactive species

Xiaojia Wang,Caroline Patenode,Bernard Roizman

Carcinogenesis 32:1427-33 PubMed21771721

2011

Inflammation-induced loss of Pdcd4 is mediated by phosphorylation-dependent degradation.

Applications

Unspecified application

Species

Unspecified reactive species

Tobias Schmid,Magdalena M Bajer,Johanna S Blees,Lisa K Eifler,Larissa Milke,Daniela Rübsamen,Kathrin Schulz,Andreas Weigert,Alyson R Baker,Nancy H Colburn,Bernhard Brüne
View all publications
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