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AB244337

Anti-PDE3A antibody

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(3 Publications)

Rabbit Polyclonal PDE3A antibody. Suitable for IHC-P, WB, ICC/IF and reacts with Human samples. Cited in 3 publications. Immunogen corresponding to Recombinant Fragment Protein within Human PDE3A aa 550-700.

View Alternative Names

Cyclic GMP-inhibited phosphodiesterase A, cGMP-inhibited cAMP phosphodiesterase, CGI-PDE A, cGI-PDE, PDE3A

5 Images
Immunocytochemistry/ Immunofluorescence - Anti-PDE3A antibody (AB244337)
  • ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-PDE3A antibody (AB244337)

PFA fixed, Triton X-100 permeabilized HeLa (Human epithelial cell line from cervix adenocarcinoma) cells labeling PDE3A using ab244337 at 4 μg/ml (green) in ICC/IF.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PDE3A antibody (AB244337)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PDE3A antibody (AB244337)

Formalin-fixed, paraffin-embedded human heart tissue stained for PDE3A with ab244337 at a 1/50 dilution in immunohistochemical analysis.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PDE3A antibody (AB244337)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PDE3A antibody (AB244337)

Formalin-fixed, paraffin-embedded human rectum tissue stained for PDE3A with ab244337 at a 1/50 dilution in immunohistochemical analysis.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PDE3A antibody (AB244337)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PDE3A antibody (AB244337)

Formalin-fixed, paraffin-embedded human placenta tissue stained for PDE3A with ab244337 at a 1/50 dilution in immunohistochemical analysis.

Western blot - Anti-PDE3A antibody (AB244337)
  • WB

Unknown

Western blot - Anti-PDE3A antibody (AB244337)

All lanes:

Western blot - Anti-PDE3A antibody (ab244337) at 0.4 µg/mL

Lane 1:

HeLa (Human epithelial cell line from cervix adenocarcinoma) cell lysate

Lane 2:

HEK-293 (Human epithelial cell line from embryonic kidney) cell lysate

Predicted band size: 125 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

IHC-P, WB, ICC/IF

applications

Immunogen

Recombinant Fragment Protein within Human PDE3A aa 550-700. The exact immunogen used to generate this antibody is proprietary information.

Q14432

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/200 - 1/500", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.04-0.4 µg/mL", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "0.25-2 µg/mL", "ICCIF-species-notes": "<p>Fixation/Permeabilization: PFA/Triton X-100.</p>" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
pH: 7.2 Preservative: 0.02% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine)
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

PDE3A also known as phosphodiesterase 3A regulates the levels of cyclic adenosine monophosphate (cAMP) and cyclic guanosine monophosphate (cGMP) in cells by hydrolyzing these cyclic nucleotides. This enzyme with a molecular mass of approximately 133 kDa is expressed in tissues including the heart vascular smooth muscle and platelets. PDE3A activity influences contractility and promotes cell cycling and differentiation through its role in modulating signal transduction pathways.
Biological function summary

Phosphodiesterase 3A plays an important role in cardiac and vascular functions. It forms part of a signaling complex responsible for regulating cardiovascular responses. PDE3A manages intracellular cAMP and cGMP concentrations impacting vascular smooth muscle relaxation and platelet aggregation. The modulation of these signaling molecules by PDE3A contributes to maintaining normal heart rhythms and influencing blood pressure.

Pathways

Phosphodiesterase 3A is central to the cAMP and cGMP signaling pathways. PDE3A activity connects to the cardiac contractility pathway where it works alongside proteins like protein kinase A (PKA) which depends on cAMP levels. In the vascular system PDE3A activity is interlinked with the nitric oxide (NO) pathway where it modulates the effects of cGMP-dependent protein kinase (PKG). Together they orchestrate vascular actions such as smooth muscle contraction and vasodilation.

Research indicates that PDE3A contributes to cardiovascular conditions like heart failure and hypertension. In heart failure altered PDE3A activity can upset cAMP levels impacting cardiac contractility. Additionally genetic variants in PDE3A have been associated with hypertension due to their influence on blood vessel dynamics. Other proteins such as PKA in cardiac tissue and PKG in vascular tissues are involved in these conditions and their regulation by PDE3A adds another layer to understanding these diseases.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Cyclic nucleotide phosphodiesterase with specificity for the second messengers cAMP and cGMP, which are key regulators of many important physiological processes (PubMed : 1315035, PubMed : 25961942, PubMed : 8155697, PubMed : 8695850). Has also activity toward cUMP (PubMed : 27975297). Independently of its catalytic activity it is part of an E2/17beta-estradiol-induced pro-apoptotic signaling pathway. E2 stabilizes the PDE3A/SLFN12 complex in the cytosol, promoting the dephosphorylation of SLFN12 and activating its pro-apoptotic ribosomal RNA/rRNA ribonuclease activity. This apoptotic pathway might be relevant in tissues with high concentration of E2 and be for instance involved in placenta remodeling (PubMed : 31420216, PubMed : 34707099).
See full target information PDE3A
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Publications (3)

Recent publications for all applications. Explore the full list and refine your search

JCI insight 9: PubMed39088302

2024

Human gut microbiota-reactive DP8α Tregs prevent acute graft-versus-host disease in a CD73-dependent manner.

Applications

Unspecified application

Species

Unspecified reactive species

Emmanuelle Godefroy,Patrice Chevallier,Fabienne Haspot,Caroline Vignes,Véronique Daguin,Sylvia Lambot,Margaux Verdon,Margaux De Seilhac,Valentin Letailleur,Anne Jarry,Annabelle Pédron,Thierry Guillaume,Pierre Peterlin,Alice Garnier,Marie-Anne Vibet,Maxence Mougon,Amandine Le Bourgeois,Maxime Jullien,Francine Jotereau,Frédéric Altare

Circulation. Heart failure 17:e011504 PubMed38910562

2024

Overexpression of in Cardiomyocytes Promotes Cardiac Reverse Remodeling.

Applications

Unspecified application

Species

Unspecified reactive species

Mengda Xu,Hang Zhang,Yuan Chang,Xiumeng Hua,Xiao Chen,Yixuan Sheng,Dan Shan,Mengni Bao,Shengshou Hu,Jiangping Song

Journal of thrombosis and haemostasis : JTH 20:1699-1711 PubMed35395698

2022

Adenosine diphosphate-induced aggregation is enhanced in platelets obtained from patients with thrombotic primary antiphospholipid syndrome (t-PAPS): Role of P2Y -cAMP signaling pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Guilherme Ruiz Leonardi,Caroline Honaiser Lescano,Jose Luiz Costa,Bruna Mazetto,Fernanda Andrade Orsi,Fabiola Zakia Monica
View all publications
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