AB315279

Anti-PDE3A antibody [EPR28228-6]

20ul selling size
Recombinant
RabMAb
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Rabbit Recombinant Monoclonal PDE3A antibody. Suitable for WB and reacts with Transfected cell lysate - Human, Human samples.

View Alternative Names

Cyclic GMP-inhibited phosphodiesterase A, cGMP-inhibited cAMP phosphodiesterase, CGI-PDE A, cGI-PDE, PDE3A

2 Images

Supplier Data

Western blot - Anti-PDE3A antibody [EPR28228-6] (AB315279)

Low expression : HEK-293 (PMID : 17124499), HuT-78 (PMID : 32005668).

The expression profile/ molecular weight observed is consistent with what has been described in the literature (PMID : 17124499).

The identity of the lower MW band at approximately 18 kDa is unknown.

This blot of lane 4 was developed using a high sensitivity ECL substrate.

In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (ab181602) staining at 1/200000 dilution.

Exposure time : Lanes 1-3 : 114 seconds; Lane 4 : 81 seconds.

All lanes:

Western blot - Anti-PDE3A antibody [EPR28228-6] (ab315279) at 1/1000 dilution

Lane 1:

HeLa (human cervical adenocarcinoma epithelial cell) whole cell lysate at 20 µg

Lane 2:

HEK-293 (human embryonic kidney epithelial cell) whole cell lysate at 20 µg

Lane 3:

HuT-78 (human sezary syndrome cutaneous t lymphocyte) whole cell lysate at 20 µg

Lane 4:

SH-SY5Y (human neuroblastoma epithelial cell) whole cell lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Observed band size: 125 kDa,90 kDa,70 kDa,36 kDa

false

Supplier Data

Western blot - Anti-PDE3A antibody [EPR28228-6] (AB315279)

This antibody does not cross-react with human PDE3B.

In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (ab181602) staining at 1/200000 dilution.

In Western blot, Anti-6X His tag® antibody [EPR20547] - ChIP Grade (ab213204) staining at 1/5000 dilution.

All lanes:

Western blot - Anti-PDE3A antibody [EPR28228-6] (ab315279) at 1/1000 dilution

Lane 1:

293T (human embryonic kidney epithelial cell) cells transfected with an empty vector containing a myc-His-tag®, whole cell lysate at 7 µg

Lane 2:

293T cells transfected with a human PDE3A expression vector containing a myc-His-tag®, whole cell lysate at 7 µg

Lane 3:

293T cells transfected with a human PDE3B expression vector containing a myc-His-tag®, whole cell lysate at 7 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Observed band size: 125 kDa,90 kDa,70 kDa,36 kDa

false

Exposure time: 6s

Carrier free

Anti-PDE3A antibody [EPR28228-6] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR28228-6

Isotype

IgG

Carrier free

Reacts with

Human

Applications

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "notRecommended", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" }, "Mouse": { "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "notRecommended", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" }, "Rat": { "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "notRecommended", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" }, "Transfected cell lysate - Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "notRecommended", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" } } }

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Product details

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Shipped at conditions

Conditional Ambient

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

PDE3A also known as phosphodiesterase 3A regulates the levels of cyclic adenosine monophosphate (cAMP) and cyclic guanosine monophosphate (cGMP) in cells by hydrolyzing these cyclic nucleotides. This enzyme with a molecular mass of approximately 133 kDa is expressed in tissues including the heart vascular smooth muscle and platelets. PDE3A activity influences contractility and promotes cell cycling and differentiation through its role in modulating signal transduction pathways.

Biological function summary

Phosphodiesterase 3A plays an important role in cardiac and vascular functions. It forms part of a signaling complex responsible for regulating cardiovascular responses. PDE3A manages intracellular cAMP and cGMP concentrations impacting vascular smooth muscle relaxation and platelet aggregation. The modulation of these signaling molecules by PDE3A contributes to maintaining normal heart rhythms and influencing blood pressure.

Pathways

Phosphodiesterase 3A is central to the cAMP and cGMP signaling pathways. PDE3A activity connects to the cardiac contractility pathway where it works alongside proteins like protein kinase A (PKA) which depends on cAMP levels. In the vascular system PDE3A activity is interlinked with the nitric oxide (NO) pathway where it modulates the effects of cGMP-dependent protein kinase (PKG). Together they orchestrate vascular actions such as smooth muscle contraction and vasodilation.

Research indicates that PDE3A contributes to cardiovascular conditions like heart failure and hypertension. In heart failure altered PDE3A activity can upset cAMP levels impacting cardiac contractility. Additionally genetic variants in PDE3A have been associated with hypertension due to their influence on blood vessel dynamics. Other proteins such as PKA in cardiac tissue and PKG in vascular tissues are involved in these conditions and their regulation by PDE3A adds another layer to understanding these diseases.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Cyclic nucleotide phosphodiesterase with specificity for the second messengers cAMP and cGMP, which are key regulators of many important physiological processes (PubMed : 1315035, PubMed : 25961942, PubMed : 8155697, PubMed : 8695850). Also has activity toward cUMP (PubMed : 27975297). Independently of its catalytic activity it is part of an E2/17beta-estradiol-induced pro-apoptotic signaling pathway. E2 stabilizes the PDE3A/SLFN12 complex in the cytosol, promoting the dephosphorylation of SLFN12 and activating its pro-apoptotic ribosomal RNA/rRNA ribonuclease activity. This apoptotic pathway might be relevant in tissues with high concentration of E2 and be for instance involved in placenta remodeling (PubMed : 31420216, PubMed : 34707099).

See full target information PDE3A

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-PDE3A antibody [EPR28228-6]

Western blot - Anti-PDE3A antibody [EPR28228-6] (AB315279)

Western blot - Anti-PDE3A antibody [EPR28228-6] (AB315279)

Related conjugates and formulations (1)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Complementary Products

Secondary Antibodies

Primary Antibodies

Primary Antibodies

Proteins & Peptides

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Product promise