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Rabbit Recombinant Monoclonal PDE3A antibody. Carrier free. Suitable for WB and reacts with Transfected cell lysate - Human, Human samples.

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Images

Western blot - Anti-PDE3A antibody [EPR28228-6] - BSA and Azide free (AB315280), expandable thumbnail
  • Western blot - Anti-PDE3A antibody [EPR28228-6] - BSA and Azide free (AB315280), expandable thumbnail

Key facts

Isotype

IgG

Host species

Rabbit

Storage buffer

pH: 7.2 - 7.4
Constituents: 100% PBS

Form

Liquid

Clonality

Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
WBIHC-PICC/IFFlow Cyt (Intra)
Human
Tested
Not recommended
Not recommended
Not recommended
Mouse
Not recommended
Not recommended
Not recommended
Not recommended
Rat
Not recommended
Not recommended
Not recommended
Not recommended
Transfected cell lysate - Human
Tested
Not recommended
Not recommended
Not recommended

Tested
Tested

Species

Transfected cell lysate - Human, Human

Dilution info

-

Notes

-

Not recommended
Not recommended

Species

Mouse, Rat

Dilution info

-

Notes

-

Not recommended
Not recommended

Species

Human, Mouse, Rat, Transfected cell lysate - Human

Dilution info

-

Notes

-

Not recommended
Not recommended

Species

Human, Transfected cell lysate - Human, Mouse, Rat

Dilution info

-

Notes

-

Not recommended
Not recommended

Species

Human, Transfected cell lysate - Human, Mouse, Rat

Dilution info

-

Notes

-

Target data

Function

Cyclic nucleotide phosphodiesterase with specificity for the second messengers cAMP and cGMP, which are key regulators of many important physiological processes (PubMed:1315035, PubMed:25961942, PubMed:8155697, PubMed:8695850). Has also activity toward cUMP (PubMed:27975297). Independently of its catalytic activity it is part of an E2/17beta-estradiol-induced pro-apoptotic signaling pathway. E2 stabilizes the PDE3A/SLFN12 complex in the cytosol, promoting the dephosphorylation of SLFN12 and activating its pro-apoptotic ribosomal RNA/rRNA ribonuclease activity. This apoptotic pathway might be relevant in tissues with high concentration of E2 and be for instance involved in placenta remodeling (PubMed:31420216, PubMed:34707099).

Alternative names

Cyclic GMP-inhibited phosphodiesterase A, cGMP-inhibited cAMP phosphodiesterase, CGI-PDE A, cGI-PDE, PDE3A

Recommended products

Rabbit Recombinant Monoclonal PDE3A antibody. Carrier free. Suitable for WB and reacts with Transfected cell lysate - Human, Human samples.

Key facts

Isotype

IgG

Form

Liquid

Clonality

Monoclonal

Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Carrier free

Yes

Clone number

EPR28228-6

Purification technique

Affinity purification Protein A

Concentration
Loading...

Storage

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Notes

ab315280 is the carrier-free version of Anti-PDE3A antibody [EPR28228-6] ab315279.

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.

Activity summary

PDE3A also known as phosphodiesterase 3A regulates the levels of cyclic adenosine monophosphate (cAMP) and cyclic guanosine monophosphate (cGMP) in cells by hydrolyzing these cyclic nucleotides. This enzyme with a molecular mass of approximately 133 kDa is expressed in tissues including the heart vascular smooth muscle and platelets. PDE3A activity influences contractility and promotes cell cycling and differentiation through its role in modulating signal transduction pathways.

Biological function summary

Phosphodiesterase 3A plays an important role in cardiac and vascular functions. It forms part of a signaling complex responsible for regulating cardiovascular responses. PDE3A manages intracellular cAMP and cGMP concentrations impacting vascular smooth muscle relaxation and platelet aggregation. The modulation of these signaling molecules by PDE3A contributes to maintaining normal heart rhythms and influencing blood pressure.

Pathways

Phosphodiesterase 3A is central to the cAMP and cGMP signaling pathways. PDE3A activity connects to the cardiac contractility pathway where it works alongside proteins like protein kinase A (PKA) which depends on cAMP levels. In the vascular system PDE3A activity is interlinked with the nitric oxide (NO) pathway where it modulates the effects of cGMP-dependent protein kinase (PKG). Together they orchestrate vascular actions such as smooth muscle contraction and vasodilation.

Associated diseases and disorders

Research indicates that PDE3A contributes to cardiovascular conditions like heart failure and hypertension. In heart failure altered PDE3A activity can upset cAMP levels impacting cardiac contractility. Additionally genetic variants in PDE3A have been associated with hypertension due to their influence on blood vessel dynamics. Other proteins such as PKA in cardiac tissue and PKG in vascular tissues are involved in these conditions and their regulation by PDE3A adds another layer to understanding these diseases.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

2 product images

  • Western blot - Anti-PDE3A antibody [EPR28228-6] - BSA and Azide free (ab315280), expandable thumbnail

    Western blot - Anti-PDE3A antibody [EPR28228-6] - BSA and Azide free (ab315280)

    This data was developed using Anti-PDE3A antibody [EPR28228-6] ab315279, the same antibody clone in a different buffer formulation.

    This antibody does not cross-react with human PDE3B.

    In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (Anti-GAPDH antibody [EPR16891] - Loading Control ab181602) staining at 1/200000 dilution.

    In Western blot, Anti-6X His tag® antibody [EPR20547] - ChIP Grade (Anti-6X His tag® antibody [EPR20547] - ChIP Grade ab213204) staining at 1/5000 dilution.

    All lanes: Western blot - Anti-PDE3A antibody [EPR28228-6] (Anti-PDE3A antibody [EPR28228-6] ab315279) at 1/1000 dilution

    Lane 1: 293T (human embryonic kidney epithelial cell) cells transfected with an empty vector containing a myc-His-tag®, whole cell lysate at 7 µg with 5% NFDM/TBST

    Lane 2: 293T cells transfected with a human PDE3A expression vector containing a myc-His-tag®, whole cell lysate at 7 µg with 5% NFDM/TBST

    Lane 3: 293T cells transfected with a human PDE3B expression vector containing a myc-His-tag®, whole cell lysate at 7 µg with 5% NFDM/TBST

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution

    Observed band size: 125 kDa, 90 kDa, 70 kDa, 36 kDa

    Exposure time: 6s

    This antibody does not cross-react with human PDE3B.

    In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (Anti-GAPDH antibody [EPR16891] - Loading Control ab181602) staining at 1/200000 dilution.

    In Western blot, Anti-6X His tag® antibody [EPR20547] - ChIP Grade (Anti-6X His tag® antibody [EPR20547] - ChIP Grade ab213204) staining at 1/5000 dilution.

  • Western blot - Anti-PDE3A antibody [EPR28228-6] - BSA and Azide free (ab315280), expandable thumbnail

    Western blot - Anti-PDE3A antibody [EPR28228-6] - BSA and Azide free (ab315280)

    This data was developed using Anti-PDE3A antibody [EPR28228-6] ab315279, the same antibody clone in a different buffer formulation.

    Low expression: HEK-293 (PMID: 17124499), HuT-78 (PMID: 32005668).

    The expression profile/ molecular weight observed is consistent with what has been described in the literature (PMID: 17124499).

    The identity of the lower MW band at approximately 18 kDa is unknown.

    This blot of lane 4 was developed using a high sensitivity ECL substrate.

    In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (Anti-GAPDH antibody [EPR16891] - Loading Control ab181602) staining at 1/200000 dilution.

    Exposure time: Lanes 1-3: 114 seconds; Lane 4: 81 seconds.

    All lanes: Western blot - Anti-PDE3A antibody [EPR28228-6] (Anti-PDE3A antibody [EPR28228-6] ab315279) at 1/1000 dilution

    Lane 1: HeLa (human cervical adenocarcinoma epithelial cell) whole cell lysate at 20 µg with 5% NFDM/TBST

    Lane 2: HEK-293 (human embryonic kidney epithelial cell) whole cell lysate at 20 µg with 5% NFDM/TBST

    Lane 3: HuT-78 (human sezary syndrome cutaneous t lymphocyte) whole cell lysate at 20 µg with 5% NFDM/TBST

    Lane 4: SH-SY5Y (human neuroblastoma epithelial cell) whole cell lysate at 20 µg with 5% NFDM/TBST

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution

    Observed band size: 125 kDa, 90 kDa, 70 kDa, 36 kDa

    Low expression: HEK-293 (PMID: 17124499), HuT-78 (PMID: 32005668).

    The expression profile/ molecular weight observed is consistent with what has been described in the literature (PMID: 17124499).

    The identity of the lower MW band at approximately 18 kDa is unknown.

    This blot of lane 4 was developed using a high sensitivity ECL substrate.

    In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (Anti-GAPDH antibody [EPR16891] - Loading Control ab181602) staining at 1/200000 dilution.

    Exposure time: Lanes 1-3: 114 seconds; Lane 4: 81 seconds.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

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