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Rabbit Recombinant Monoclonal PDE3B antibody. Suitable for Dot, WB, IP and reacts with Recombinant fragment - Human, Human samples.

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Images

Western blot - Anti-PDE3B antibody [EPR28229-71] (AB317026), expandable thumbnail
  • Western blot - Anti-PDE3B antibody [EPR28229-71] (AB317026), expandable thumbnail
  • Western blot - Anti-PDE3B antibody [EPR28229-71] (AB317026), expandable thumbnail
  • Dot Blot - Anti-PDE3B antibody [EPR28229-71] (AB317026), expandable thumbnail
  • Immunoprecipitation - Anti-PDE3B antibody [EPR28229-71] (AB317026), expandable thumbnail

Key facts

Isotype
IgG
Host species
Rabbit
Storage buffer

pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Form
Liquid
Clonality
Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
DotWBIPIHC-P
Human
Expected
Tested
Tested
Not recommended
Mouse
Not recommended
Not recommended
Not recommended
Not recommended
Rat
Not recommended
Not recommended
Not recommended
Not recommended
Recombinant fragment - Human
Tested
Not recommended
Not recommended
Not recommended

Tested
Tested

Species
Recombinant fragment - Human
Dilution info
1/1000
Notes

-

Expected
Expected

Species
Human
Dilution info
Use at an assay dependent concentration.
Notes

-

Not recommended
Not recommended

Species
Mouse, Rat
Dilution info
-
Notes

-

Tested
Tested

Species
Human
Dilution info
1/1000
Notes

-

Not recommended
Not recommended

Species
Mouse, Rat, Recombinant fragment - Human
Dilution info
-
Notes

-

Tested
Tested

Species
Human
Dilution info
1/30
Notes

-

Not recommended
Not recommended

Species
Recombinant fragment - Human, Mouse, Rat
Dilution info
-
Notes

-

Not recommended
Not recommended

Species
Human, Mouse, Rat, Recombinant fragment - Human
Dilution info
-
Notes

-

Target data

Function

Cyclic nucleotide phosphodiesterase with a dual-specificity for the second messengers cAMP and cGMP, which are key regulators of many important physiological process (PubMed:14592490, PubMed:21393242). Regulates angiogenesis by inhibiting the cAMP-dependent guanine nucleotide exchange factor RAPGEF3 and downstream phosphatidylinositol 3-kinase gamma-mediated signaling (PubMed:21393242). Controls cardiac contractility by reducing cAMP concentration in cardiocytes (By similarity).

Alternative names

CGIPDE1, Cyclic GMP-inhibited phosphodiesterase B, CGIP1, CGI-PDE B, PDE3B

Recommended products

Rabbit Recombinant Monoclonal PDE3B antibody. Suitable for Dot, WB, IP and reacts with Recombinant fragment - Human, Human samples.

Key facts

Isotype
IgG
Form
Liquid
Clonality
Monoclonal
Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Clone number
EPR28229-71
Purification technique
Affinity purification Protein A
Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle

Notes

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.
Activity summary

PDE3B also known as cGMP-inhibited phosphodiesterase B is an enzyme with a role in cyclic nucleotide signaling. It possesses a molecular mass of approximately 133 kDa. PDE3B predominantly gets expressed in adipose tissue liver and to some extent in the heart. It breaks down cyclic adenosine monophosphate (cAMP) and cyclic guanosine monophosphate (cGMP) which play key roles in cellular signaling. By hydrolyzing these cyclic nucleotides into their non-cyclic forms PDE3B regulates intracellular levels of these molecules.

Biological function summary

In adipose tissue and liver PDE3B modulates lipogenesis and lipolysis which are vital processes for energy balance. This enzyme interacts with signaling pathways that involve protein kinase A (PKA) and insulin signaling. PDE3B operates as part of larger protein complexes and influences the metabolic rate by regulating the availability of the cAMP for phosphorylation reactions. The activity of PDE3B links closely to the control of energy homeostasis due to its regulatory function on lipid metabolism.

Pathways

PDE3B is integral to the insulin signaling pathway and adiponectin signaling pathway both important in glucose and lipid homeostasis. Within these pathways it functions alongside proteins like AKT and PKA where its activity modulates their signaling efficiency. By controlling the balance of cAMP PDE3B influences signaling cascades that are important for the adaptive cellular responses to hormones and nutrients.

Associated diseases and disorders

PDE3B connects to conditions such as obesity and type 2 diabetes due to its involvement in energy homeostasis and lipid metabolism. In type 2 diabetes the dysregulation of PDE3B activity affects insulin signaling and glucose uptake. Additionally in the context of obesity PDE3B activity shifts the balance of lipolysis and lipogenesis influencing adipose tissue mass and function. Proteins such as insulin receptors and PKA become closely tied to PDE3B in these disorders highlighting its role in metabolic disease mechanisms.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

5 product images

  • Western blot - Anti-PDE3B antibody [EPR28229-71] (ab317026), expandable thumbnail

    Western blot - Anti-PDE3B antibody [EPR28229-71] (ab317026)

    Lysates were freshly made and used for Western blotting immediately to minimize protein degradation.

    The identity of the lower MW band at approximately 20 kDa is unknown.

    In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (Anti-GAPDH antibody [EPR16891] - Loading Control ab181602) staining at 1/200000 dilution.

    All lanes: Western blot - Anti-PDE3B antibody [EPR28229-71] (ab317026) at 1/1000 dilution

    Lane 1: HeLa (human cervical adenocarcinoma epithelial cell) transfected with scrambled siRNA control whole cell lysate at 20 µg with 5% NFDM/TBST

    Lane 2: HeLa transfected with siRNA specifically targeting PDE3B whole cell lysate at 20 µg with 5% NFDM/TBST

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution

    Observed band size: 145 kDa, 36 kDa

    Exposure time: 59s

  • Western blot - Anti-PDE3B antibody [EPR28229-71] (ab317026), expandable thumbnail

    Western blot - Anti-PDE3B antibody [EPR28229-71] (ab317026)

    Lysates were freshly made and used for Western blotting immediately to minimize protein degradation.

    The identity of the lower MW band at approximately 20 kDa is unknown.

    In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (Anti-GAPDH antibody [EPR16891] - Loading Control ab181602) staining at 1/200000 dilution.

    All lanes: Western blot - Anti-PDE3B antibody [EPR28229-71] (ab317026) at 1/1000 dilution

    Lane 1: HeLa (human cervical adenocarcinoma epithelial cell) whole cell lysate at 20 µg with 5% NFDM/TBST

    Lane 2: 293T (human embryonic kidney epithelial cell) whole cell lysate at 20 µg with 5% NFDM/TBST

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution

    Observed band size: 145 kDa, 36 kDa

    Exposure time: 15s

  • Western blot - Anti-PDE3B antibody [EPR28229-71] (ab317026), expandable thumbnail

    Western blot - Anti-PDE3B antibody [EPR28229-71] (ab317026)

    Low expression: human skeletal muscle.

    The identity of the lower MW band at approximately 20 kDa is unknown.

    In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (Anti-GAPDH antibody [EPR16891] - Loading Control ab181602) staining at 1/200000 dilution.

    All lanes: Western blot - Anti-PDE3B antibody [EPR28229-71] (ab317026) at 1/1000 dilution

    Lane 1: Human adipocytes tissue lysate at 20 µg with 5% NFDM/TBST

    Lane 2: Human liver tissue lysate at 20 µg with 5% NFDM/TBST

    Lane 3: Human skeletal muscle tissue lysate at 20 µg with 5% NFDM/TBST

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution

    Observed band size: 145 kDa, 36 kDa

    Exposure time: 70s

  • Dot Blot - Anti-PDE3B antibody [EPR28229-71] (ab317026), expandable thumbnail

    Dot Blot - Anti-PDE3B antibody [EPR28229-71] (ab317026)

    Dot blot analysis of PDE3B using ab317026 at 1:1000 (0.498 ug/ml) followed by a Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1:100,000 dilution.

    This antibody does not cross-react with human PDE3A.

    All lanes: Dot Blot - Anti-PDE3B antibody [EPR28229-71] (ab317026) at 1/1000 dilution

    Lane 1: His-tagged human PDE3B protein with 5% NFDM/TBST

    Lane 2: His-tagged human PDE3A protein with 5% NFDM/TBST

    Secondary

    All lanes: Dot Blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution

    Exposure time: 180s

  • Immunoprecipitation - Anti-PDE3B antibody [EPR28229-71] (ab317026), expandable thumbnail

    Immunoprecipitation - Anti-PDE3B antibody [EPR28229-71] (ab317026)

    PDE3B was immunoprecipitated from 0.35 mg HeLa (human cervix adenocarcinoma epithelial cell) whole cell lysate with ab317026 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab317026 at 1/1000 dilution. VeriBlot for IP secondary antibody(HRP)(VeriBlot for IP Detection Reagent (HRP) ab131366) was used at 1/5000 dilution.

    Lane 1: HeLa (human cervix adenocarcinoma epithelial cell) whole cell lysate

    Lane 2: ab317026 IP in HeLa (human cervix adenocarcinoma epithelial cell) whole cell lysate

    Lane 3: Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) instead of ab317026 in HeLa whole cell lysate

    Lysates were freshly made and used for Western blotting immediately to minimize protein degradation.

    All lanes: Immunoprecipitation - Anti-PDE3B antibody [EPR28229-71] (ab317026) at 1/30 dilution

    All lanes: HeLa (human cervix adenocarcinoma epithelial cell) whole cell lysate with 5% NFDM/TBST

    Secondary

    All lanes: Immunoprecipitation - VeriBlot for IP Detection Reagent (HRP) (VeriBlot for IP Detection Reagent (HRP) ab131366) at 1/5000 dilution

    Exposure time: 24s

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

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