AB14628

Anti-PDE4 antibody

Name: Anti-PDE4 antibody (ab14628) | Abcam
Brand: Abcam Limited
SKU: AB14628

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(23 Publications)

Rabbit Polyclonal PDE4D antibody. Suitable for WB, ICC/IF and reacts with Rat, Human, Mouse samples. Cited in 23 publications.

View Alternative Names

DPDE3, cAMP-specific phosphodiesterase 4D, Pde4d, DPDE2, cAMP-specific phosphodiesterase 4A, Pde4a

2 Images

Immunocytochemistry/ Immunofluorescence - Anti-PDE4 antibody (AB14628)

ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-PDE4 antibody (AB14628)

ICC/IF image of ab14628 stained PC12 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab14628, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

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Western blot - Anti-PDE4 antibody (AB14628)

ab 14628 detecting 3 isoforms of PDE4A subclasses (PDE4A1, PDE4A5 and PDE4Ax), two isoforms of PDE4D (PDE4D1, PDE4D3) in rat brain by Western blot.

ab 14628 detecting 3 isoforms of PDE4A subclasses (PDE4A1, PDE4A5 and PDE4Ax) and two isoforms of PDE4D (PDE4D1, PDE4D3) in rat brain by Western blot.

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Western blot - Anti-PDE4 antibody (ab14628)

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Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Rat, Human, Mouse

Applications

WB, ICC/IF

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

Specificity

This antibody detects all known PDE4 A and D variants including PDE4A1, A5, Ax, A8 and PDE4D1-D5. It has low affinity for PDE4B variants.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "1 µg/mL", "ICCIF-species-notes": "" }, "Mouse": { "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "1 µg/mL", "ICCIF-species-notes": "" }, "Rat": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1 µg/mL", "ICCIF-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Storage buffer

Preservative: 0.02% Thimerosal (merthiolate)

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

PDE4 or phosphodiesterase 4 is an enzyme that specifically hydrolyzes cyclic adenosine monophosphate (cAMP) into AMP which regulates intracellular levels of cAMP. PDE4 subfamily includes four isoforms: PDE4A PDE4B PDE4C and PDE4D each with unique expression patterns and roles. The approximate molecular weight of PDE4 enzymes varies depending on isoform but typically around 80-100 kDa. This enzyme is widely distributed in the body with expression found primarily in the brain heart lungs and immune cells where it modulates cellular responses to hormonal signals.

Biological function summary

PDE4 plays an important role in modulating inflammatory responses and immune cell activation due to its control over cAMP levels. By degrading cAMP PDE4 impacts the signaling pathways that govern inflammation and immune responses. This enzyme is often found in complex with other proteins that help localize and regulate its activity within cells. It plays a significant role in neuron communication and neuroplasticity affecting learning and memory processes.

Pathways

PDE4 is integral to the cAMP signaling pathway which impacts numerous physiological processes including cardiac function and immune responses. It interacts closely with protein kinase A (PKA) as its downstream effector thereby influencing PKA-dependent cellular responses. PDE4 also interacts within the mitogen-activated protein kinase (MAPK) pathway linking it to cell growth and differentiation responses which are important for various cellular processes.

PDE4 is strongly implicated in inflammatory and neuropsychiatric conditions. Overactivity or dysregulation of PDE4 can lead to disorders such as asthma and chronic obstructive pulmonary disease (COPD) due to its role in inflammation. It is also connected to major depressive disorder where altered cAMP signaling plays a part in pathophysiology. Inflammatory responses are linked to proteins such as tumor necrosis factor-alpha (TNF-alpha) and interleukins where PDE4 modulation can influence these pathways affecting disease development and progression.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Hydrolyzes the second messenger cAMP, which is a key regulator of many important physiological processes.

See full target information Pde4d

Additional targets

Pde4a

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Publications (23)

Recent publications for all applications. Explore the full list and refine your search

Scientific reports 14:24875 PubMed39438515

2024

Validation of a functional human AD model with four AD therapeutics utilizing patterned ipsc-derived cortical neurons integrated with microelectrode arrays.

Applications

Unspecified application

Species

Unspecified reactive species

Julbert Caneus,Kaveena Autar,Nesar Akanda,Marcella Grillo,Christopher J Long,Max Jackson,Sarah Lindquist,Xiufang Guo,Dave Morgan,James J Hickman

Experimental brain research 242:309-320 PubMed38052997

2023

Effects of exercise-targeted hippocampal PDE-4 methylation on synaptic plasticity and spatial learning/memory impairments in D-galactose-induced aging rats.

Applications

Unspecified application

Species

Unspecified reactive species

Yu Jin,Xue Li,Changling Wei,Qiongjia Yuan

Arthritis & rheumatology (Hoboken, N.J.) 75:1628-1637 PubMed36862398

2023

Reduction of Neutrophil Activation by Phosphodiesterase 4 Blockade in Behçet's Disease.

Applications

Unspecified application

Species

Unspecified reactive species

Alexandre Le Joncour,Paul Régnier,Anna Maciejewski-Duval,Erwan Charles,Stéphane Barete,Pierre Fouret,Michelle Rosenzwajg,David Klatzmann,Patrice Cacoub,David Saadoun

Science signaling 16:eade3380 PubMed36976866

2023

Arrestin-dependent nuclear export of phosphodiesterase 4D promotes GPCR-induced nuclear cAMP signaling required for learning and memory.

Applications

Unspecified application

Species

Unspecified reactive species

Joseph M Martinez,Ao Shen,Bing Xu,Aleksandra Jovanovic,Josephine de Chabot,Jin Zhang,Yang K Xiang

Drug development research 84:226-237 PubMed36621953

2023

PDE4 inhibitor Roflumilast modulates inflammation and lipid accumulation in PCOS mice to improve ovarian function and reduce DHEA-induced granulosa cell apoptosis in vitro.

Applications

Unspecified application

Species

Unspecified reactive species

Xiaowei Ji,Yao Ye,Lin Wang,Suying Liu,Xi Dong

Frontiers in aging neuroscience 14:811481 PubMed35615594

2022

HIV-1 gp120 Impairs Spatial Memory Through Cyclic AMP Response Element-Binding Protein.

Applications

Unspecified application

Species

Unspecified reactive species

Jenny Shrestha,Maryline Santerre,Charles N S Allen,Sterling P Arjona,Carmen Merali,Ruma Mukerjee,Kumaraswamy Naidu Chitrala,Jin Park,Asen Bagashev,Viet Bui,Eliseo A Eugenin,Salim Merali,Marcus Kaul,Jeannie Chin,Bassel E Sawaya

Experimental and therapeutic medicine 23:340 PubMed35401806

2022

Transcription factor JDP2 activates PDE4B to participate in hypoxia/reoxygenation-induced H9c2 cell injury.

Applications

Unspecified application

Species

Unspecified reactive species

Suipeng Li,Yong Chen,Yinfeng Jia,Tingting Xue,Xuqing Hou,Zhangyin Zhao

Scientific reports 11:19737 PubMed34611179

2021

The protective effect of the PDE-4 inhibitor rolipram on intracerebral haemorrhage is associated with the cAMP/AMPK/SIRT1 pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Xiao-Liu Dong,Yan-Hui Wang,Jing Xu,Nan Zhang

Experimental and therapeutic medicine 22:1398 PubMed34650646

2021

Roflumilast, a type of phosphodiesterase 4 inhibitor, can reduce intestinal injury caused by sepsis.

Applications

Unspecified application

Species

Unspecified reactive species

Zhongyuan Zhang,Meifeng Liang,Xiongfei Wan

Immunity 50:152-165.e8 PubMed30611611

2019

Disruption of Coronin 1 Signaling in T Cells Promotes Allograft Tolerance while Maintaining Anti-Pathogen Immunity.

Applications

Unspecified application

Species

Unspecified reactive species

Rajesh Jayachandran,Aleksandra Gumienny,Beatrice Bolinger,Sebastian Ruehl,Mathias Jakob Lang,Geoffrey Fucile,Saumyabrata Mazumder,Vincent Tchang,Anne-Kathrin Woischnig,Michael Stiess,Gabriele Kunz,Beatrice Claudi,Mathias Schmaler,Kerstin Siegmund,Jianping Li,Simone Dertschnig,George Holländer,Eva Medina,Urs Karrer,Despina Moshous,Dirk Bumann,Nina Khanna,Simona W Rossi,Jean Pieters

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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Anti-PDE4 antibody

Immunocytochemistry/ Immunofluorescence - Anti-PDE4 antibody (AB14628)

Western blot - Anti-PDE4 antibody (AB14628)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Specificity

Alternative Products

Primary Antibodies

Complementary Products

Primary Antibodies

Primary Antibodies

Secondary Antibodies

Primary Antibodies

Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Additional targets

Publications (23)

Product promise