Rabbit Polyclonal PDE4D antibody. Suitable for WB, ICC/IF and reacts with Rat, Human, Mouse samples. Cited in 21 publications.
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- Experimental brain research 242:309-3202023Effects of exercise-targeted hippocampal PDE-4 methylation on synaptic plasticity and spatial learning/memory impairments in D-galactose-induced aging rats.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYu Jin,Xue Li,Changling Wei,Qiongjia YuanPubMed 38052997
- Arthritis & rheumatology (Hoboken, N.J.) 75:1628-16372023Reduction of Neutrophil Activation by Phosphodiesterase 4 Blockade in Behçet's Disease.Applications:Unspecified applicationReactive species:Unspecified reactive speciesAlexandre Le Joncour,Paul Régnier,Anna Maciejewski-Duval,Erwan Charles,Stéphane Barete,Pierre Fouret,Michelle Rosenzwajg,David Klatzmann,Patrice Cacoub,David SaadounPubMed 36862398
- Science signaling 16:eade33802023Arrestin-dependent nuclear export of phosphodiesterase 4D promotes GPCR-induced nuclear cAMP signaling required for learning and memory.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJoseph M Martinez,Ao Shen,Bing Xu,Aleksandra Jovanovic,Josephine de Chabot,Jin Zhang,Yang K XiangPubMed 36976866
- Frontiers in aging neuroscience 14:8114812022HIV-1 gp120 Impairs Spatial Memory Through Cyclic AMP Response Element-Binding Protein.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJenny Shrestha,Maryline Santerre,Charles N S Allen,Sterling P Arjona,Carmen Merali,Ruma Mukerjee,Kumaraswamy Naidu Chitrala,Jin Park,Asen Bagashev,Viet Bui,Eliseo A Eugenin,Salim Merali,Marcus Kaul,Jeannie Chin,Bassel E SawayaPubMed 35615594
- Experimental and therapeutic medicine 23:3402022Transcription factor JDP2 activates PDE4B to participate in hypoxia/reoxygenation-induced H9c2 cell injury.Applications:Unspecified applicationReactive species:Unspecified reactive speciesSuipeng Li,Yong Chen,Yinfeng Jia,Tingting Xue,Xuqing Hou,Zhangyin ZhaoPubMed 35401806
- Scientific reports 11:197372021The protective effect of the PDE-4 inhibitor rolipram on intracerebral haemorrhage is associated with the cAMP/AMPK/SIRT1 pathway.Applications:Unspecified applicationReactive species:Unspecified reactive speciesXiao-Liu Dong,Yan-Hui Wang,Jing Xu,Nan ZhangPubMed 34611179
- Experimental and therapeutic medicine 22:13982021Roflumilast, a type of phosphodiesterase 4 inhibitor, can reduce intestinal injury caused by sepsis.Applications:Unspecified applicationReactive species:Unspecified reactive speciesZhongyuan Zhang,Meifeng Liang,Xiongfei WanPubMed 34650646
- Immunity 50:152-165.e82019Disruption of Coronin 1 Signaling in T Cells Promotes Allograft Tolerance while Maintaining Anti-Pathogen Immunity.Applications:Unspecified applicationReactive species:Unspecified reactive speciesRajesh Jayachandran et. al.PubMed 30611611
- Frontiers in neuroscience 12:3372018Reduced Cyclic Adenosine Monophosphate Level in Hippocampal CA1 Participates in Propofol Induced Amnesia in Rats.Applications:Unspecified applicationReactive species:Unspecified reactive speciesWeiwei Li et. al.PubMed 29875624
- Journal of cellular physiology 233:7239-72522018Cyclic AMP and glycogen synthase kinase 3 form a regulatory loop in spermatozoa.Applications:Unspecified applicationReactive species:Unspecified reactive speciesSouvik Dey,Suranjana Goswami,Alaa Eisa,Rahul Bhattacharjee,Cameron Brothag,Douglas Kline,Srinivasan VijayaraghavanPubMed 29574946
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
Preservative: 0.02% Thimerosal (merthiolate)
- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
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Reactivity data
Select an application| WB | ICC/IF | |
|---|---|---|
| Human | Expected | Expected |
| Mouse | Expected | Expected |
| Rat | Tested | Tested |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Rat | Dilution info - | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Human, Mouse | Dilution info Use at an assay dependent concentration. | Notes - |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Rat | Dilution info 1 µg/mL | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info 1 µg/mL | Notes - |
Species Human | Dilution info 1 µg/mL | Notes - |
Target data
Function
Hydrolyzes the second messenger cAMP, which is a key regulator of many important physiological processes.
Additional Targets
Pde4a
Alternative names
DPDE3, cAMP-specific phosphodiesterase 4D, Pde4d
Recommended products
Rabbit Polyclonal PDE4D antibody. Suitable for WB, ICC/IF and reacts with Rat, Human, Mouse samples. Cited in 21 publications.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
Preservative: 0.02% Thimerosal (merthiolate)
- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Purification technique
- Affinity purification Immunogen
- Specificity
This antibody detects all known PDE4 A and D variants including PDE4A1, A5, Ax, A8 and PDE4D1-D5. It has low affinity for PDE4B variants.
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
PDE4 or phosphodiesterase 4 is an enzyme that specifically hydrolyzes cyclic adenosine monophosphate (cAMP) into AMP which regulates intracellular levels of cAMP. PDE4 subfamily includes four isoforms: PDE4A PDE4B PDE4C and PDE4D each with unique expression patterns and roles. The approximate molecular weight of PDE4 enzymes varies depending on isoform but typically around 80-100 kDa. This enzyme is widely distributed in the body with expression found primarily in the brain heart lungs and immune cells where it modulates cellular responses to hormonal signals.
- Biological function summary
PDE4 plays an important role in modulating inflammatory responses and immune cell activation due to its control over cAMP levels. By degrading cAMP PDE4 impacts the signaling pathways that govern inflammation and immune responses. This enzyme is often found in complex with other proteins that help localize and regulate its activity within cells. It plays a significant role in neuron communication and neuroplasticity affecting learning and memory processes.
- Pathways
PDE4 is integral to the cAMP signaling pathway which impacts numerous physiological processes including cardiac function and immune responses. It interacts closely with protein kinase A (PKA) as its downstream effector thereby influencing PKA-dependent cellular responses. PDE4 also interacts within the mitogen-activated protein kinase (MAPK) pathway linking it to cell growth and differentiation responses which are important for various cellular processes.
- Associated diseases and disorders
PDE4 is strongly implicated in inflammatory and neuropsychiatric conditions. Overactivity or dysregulation of PDE4 can lead to disorders such as asthma and chronic obstructive pulmonary disease (COPD) due to its role in inflammation. It is also connected to major depressive disorder where altered cAMP signaling plays a part in pathophysiology. Inflammatory responses are linked to proteins such as tumor necrosis factor-alpha (TNF-alpha) and interleukins where PDE4 modulation can influence these pathways affecting disease development and progression.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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2 product images
Western blot - Anti-PDE4 antibody (ab14628)
ab 14628 detecting 3 isoforms of PDE4A subclasses (PDE4A1, PDE4A5 and PDE4Ax), two isoforms of PDE4D (PDE4D1, PDE4D3) in rat brain by Western blot.
ab 14628 detecting 3 isoforms of PDE4A subclasses (PDE4A1, PDE4A5 and PDE4Ax) and two isoforms of PDE4D (PDE4D1, PDE4D3) in rat brain by Western blot.
All lanes: Western blot - Anti-PDE4 antibody (ab14628)
Immunocytochemistry/ Immunofluorescence - Anti-PDE4 antibody (ab14628)
ICC/IF image of ab14628 stained PC12 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab14628, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
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