Rabbit Polyclonal PDE4A antibody. Suitable for IHC-P, WB and reacts with Human samples. Cited in 4 publications. Immunogen corresponding to Synthetic Peptide within Human PDE4A.
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- JCI insight 8:2023Inhibition of phosphodiesterase 4D suppresses mTORC1 signaling and pancreatic cancer growth.Applications:Unspecified applicationReactive species:Unspecified reactive speciesMi-Hyeon Jeong,Greg Urquhart,Cheryl Lewis,Zhikai Chi,Jenna L JewellPubMed 37427586
- Neurochemical research 47:1707-17202022CircAXL Knockdown Alleviates Aβ-Induced Neurotoxicity in Alzheimer's Disease via Repressing PDE4A by Releasing miR-1306-5p.Applications:Unspecified applicationReactive species:Unspecified reactive speciesShengxi Meng,Bing Wang,Wentao LiPubMed 35229272
- Journal of cellular and molecular medicine 25:3460-34682021H S catalysed by CBS regulates testosterone synthesis through affecting the sulfhydrylation of PDE.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJing Wang,Jing Wang,Tao Shen,Renyun Hong,Shanshan Tang,Xia ZhaoPubMed 33713531
- PLoS biology 16:e20044082018CDKN1B/p27 is localized in mitochondria and improves respiration-dependent processes in the cardiovascular system-New mode of action for caffeine.Applications:Unspecified applicationReactive species:Unspecified reactive speciesNiloofar Ale-Agha et. al.PubMed 29927970
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.4
Preservative: 0.05% Sodium azide
Constituents: 50% Glycerol (glycerin, glycerine), 49% PBS- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- Synthetic Peptide within Human PDE4A. The exact immunogen used to generate this antibody is proprietary information. Database link P27815
Reactivity data
Select an application| IHC-P | WB | |
|---|---|---|
| Human | Tested | Tested |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/50.00000 - 1/200.00000 | Notes Perform heat-mediated antigen retrieval before commencing with IHC staining protocol. |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/500.00000 - 1/2000.00000 | Notes - |
Target data
Function
Hydrolyzes the second messenger 3',5'-cyclic AMP (cAMP), which is a key regulator of many important physiological processes. Isoform 1. Efficiently hydrolyzes cAMP. Isoform 2. Efficiently hydrolyzes cAMP. Isoform 3. Efficiently hydrolyzes cAMP. The phosphodiesterase activity is not affected by calcium, calmodulin or cyclic GMP (cGMP) levels. Does not hydrolyze cGMP. Isoform 4. Efficiently hydrolyzes cAMP. Isoform 6. Efficiently hydrolyzes cAMP. Isoform 7. Efficiently hydrolyzes cAMP.
Alternative names
DPDE2, PDE4A, PDE46, cAMP-specific phosphodiesterase 4A
Recommended products
Rabbit Polyclonal PDE4A antibody. Suitable for IHC-P, WB and reacts with Human samples. Cited in 4 publications. Immunogen corresponding to Synthetic Peptide within Human PDE4A.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.4
Preservative: 0.05% Sodium azide
Constituents: 50% Glycerol (glycerin, glycerine), 49% PBS- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- Synthetic Peptide within Human PDE4A. The exact immunogen used to generate this antibody is proprietary information. Database link P27815
- Purification technique
- Affinity purification Immunogen
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
PDE4A or phosphodiesterase 4A is an isoform part of the PDE4 family known for hydrolyzing cAMP an important second messenger in cells. The mass of PDE4A can vary depending on the isoform with commonly referenced masses around 70-100 kDa. This enzyme is expressed in various tissues including the brain immune cells and lungs. Other popular names for this protein include PDE46 and DPDE2 indicating its different isoforms and specific functionalities across tissue types.
- Biological function summary
PDE4A plays an important role in regulating intracellular levels of cyclic adenosine monophosphate (cAMP) a critical component in signaling pathways that affect numerous cellular processes. By controlling cAMP concentrations PDE4A regulates inflammatory responses and airway smooth muscle contraction which is important for respiratory functions. PDE4A does not function within a complex but operates as a homodimer when exerting its effects on signaling cascades.
- Pathways
PDE4A's role is significant in the cAMP-dependent signaling pathway. This pathway influences processes like inflammation and smooth muscle relaxation. PDE4A interacts with various proteins such as PKA (protein kinase A) which is activated by cAMP and mediates several downstream effects. By influencing the cAMP pathway PDE4A affects bodily responses including immune and respiratory systems.
- Associated diseases and disorders
PDE4A has implications in conditions such as asthma and chronic obstructive pulmonary disease (COPD). Dysregulation of cAMP through PDE4A can contribute to these respiratory conditions by affecting airway inflammation and muscle tone. PDE4A is also linked to other related proteins such as PDE4B which can also impact inflammatory pathways in similar disease contexts. PDE4 inhibitors have been explored as therapeutic agents for modifying these diseases by targeting the enzymatic activity of PDE4A and related isoforms to restore balanced cAMP levels.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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2 product images
Western blot - Anti-PDE4A antibody (ab200383)
6% SDS-PAGE.
All lanes: Western blot - Anti-PDE4A antibody (ab200383) at 1/300 dilution
Lane 1: HeLa cell lysate at 40 µg
Lane 2: A172 cell lysate at 40 µg
Predicted band size: 98 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PDE4A antibody (ab200383)
Immunohistochemical analysis of paraffin-embedded Human thyroid cancer tissue labeling PDE4A with ab200383 at 1/40 dilution.
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