Rabbit Recombinant Monoclonal PDE4A antibody. Carrier free. Suitable for sELISA and reacts with Human samples.
Images
![Sandwich ELISA - Anti-PDE4A antibody [EPR26991-93] - BSA and Azide free (Capture) (AB313719), expandable thumbnail](https://content.abcam.com/products/images/pde4a-antibody-epr26991-93-bsa-and-azide-free-capture-ab313719--sandwich-elisa-img419280.jpg/_jcr_content/renditions/webp-475.webp "Sandwich ELISA - Anti-PDE4A antibody [EPR26991-93] - BSA and Azide free (Capture) (AB313719)")
Key facts
- Isotype
IgG
- Host species
Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: 100% PBS- Form
Liquid
- Clonality
Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| sELISA | |
|---|---|
| Human | Tested |
sELISA
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
Target data
Function
Hydrolyzes the second messenger 3',5'-cyclic AMP (cAMP), which is a key regulator of many important physiological processes.Isoform 1Efficiently hydrolyzes cAMP.Isoform 2Efficiently hydrolyzes cAMP.Isoform 3Efficiently hydrolyzes cAMP. The phosphodiesterase activity is not affected by calcium, calmodulin or cyclic GMP (cGMP) levels. Does not hydrolyze cGMP.Isoform 4Efficiently hydrolyzes cAMP.Isoform 6Efficiently hydrolyzes cAMP.Isoform 7Efficiently hydrolyzes cAMP.
Alternative names
PDE4A, PDE4A, DPDE2, DPDE2, PDE46, cAMP-specific phosphodiesterase 4A
Recommended products
Rabbit Recombinant Monoclonal PDE4A antibody. Carrier free. Suitable for sELISA and reacts with Human samples.
Alternative names
PDE4A, PDE4A, DPDE2, DPDE2, PDE46, cAMP-specific phosphodiesterase 4A
Key facts
- Isotype
IgG
- Host species
Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: 100% PBS- Form
Liquid
- Clonality
Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Carrier free
Yes
- Clone number
EPR26991-93
- Concentration
Storage
- Shipped at conditions
Blue Ice
- Appropriate long-term storage conditions
+4°C
Notes
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
Supplementary info
- Activity summary
PDE4A or phosphodiesterase 4A is an isoform part of the PDE4 family known for hydrolyzing cAMP an important second messenger in cells. The mass of PDE4A can vary depending on the isoform with commonly referenced masses around 70-100 kDa. This enzyme is expressed in various tissues including the brain immune cells and lungs. Other popular names for this protein include PDE46 and DPDE2 indicating its different isoforms and specific functionalities across tissue types.
- Biological function summary
PDE4A plays an important role in regulating intracellular levels of cyclic adenosine monophosphate (cAMP) a critical component in signaling pathways that affect numerous cellular processes. By controlling cAMP concentrations PDE4A regulates inflammatory responses and airway smooth muscle contraction which is important for respiratory functions. PDE4A does not function within a complex but operates as a homodimer when exerting its effects on signaling cascades.
- Pathways
PDE4A's role is significant in the cAMP-dependent signaling pathway. This pathway influences processes like inflammation and smooth muscle relaxation. PDE4A interacts with various proteins such as PKA (protein kinase A) which is activated by cAMP and mediates several downstream effects. By influencing the cAMP pathway PDE4A affects bodily responses including immune and respiratory systems.
- Associated diseases and disorders
PDE4A has implications in conditions such as asthma and chronic obstructive pulmonary disease (COPD). Dysregulation of cAMP through PDE4A can contribute to these respiratory conditions by affecting airway inflammation and muscle tone. PDE4A is also linked to other related proteins such as PDE4B which can also impact inflammatory pathways in similar disease contexts. PDE4 inhibitors have been explored as therapeutic agents for modifying these diseases by targeting the enzymatic activity of PDE4A and related isoforms to restore balanced cAMP levels.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
1 product image
![Sandwich ELISA - Anti-PDE4A antibody [EPR26991-93] - BSA and Azide free (Capture) (ab313719), expandable thumbnail](https://content.abcam.com/products/images/pde4a-antibody-epr26991-93-bsa-and-azide-free-capture-ab313719--sandwich-elisa-img419280.jpg "Sandwich ELISA - Anti-PDE4A antibody [EPR26991-93] - BSA and Azide free (Capture) (ab313719)")
Sandwich ELISA - Anti-PDE4A antibody [EPR26991-93] - BSA and Azide free (Capture) (ab313719)
Sandwich ELISA of Human PDE4A Antibody Pair - BSA and Azide free ab313718 with the capture antibody dilution at 2 µg/mL and detector antibody dilution at 0.5 µg/mL.
Downloads
Product protocols
- Visit the general protocols
- Visit troubleshooting
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