AB112014

Anti-PDE4B antibody

Name: Anti-PDE4B antibody (ab112014) | Abcam
Brand: Abcam Limited
SKU: AB112014
Price: 565 USD
Availability: InStock

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(3 Publications)

Goat Polyclonal PDE4B3 antibody. Suitable for IHC-P, WB and reacts with Human, Mouse samples. Cited in 3 publications. Immunogen corresponding to Synthetic Peptide within Human PDE4B aa 700-750.

View Alternative Names

DPDE4, PDE4B, PDE32, cAMP-specific phosphodiesterase 4B

2 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PDE4B antibody (AB112014)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PDE4B antibody (AB112014)

ab112014, at 5µg/ml, staining PDE4B in formalin-fixed, paraffin-embedded Human Prostate tissue by Immunohistochemistry.

Unknown

Western blot - Anti-PDE4B antibody (AB112014)

All lanes:

Western blot - Anti-PDE4B antibody (ab112014) at 0.5 µg/mL

All lanes:

Mouse Brain lysate (in RIPA buffer) at 35 µg

Predicted band size: 83 kDa

true

Key facts

Host species

Goat

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Mouse, Human

Applications

IHC-P, WB

applications

Immunogen

Synthetic Peptide within Human PDE4B aa 700-750. The exact immunogen used to generate this antibody is proprietary information.

Q07343

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "5 µg/mL", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Mouse": { "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.5-1 µg/mL", "WB-species-notes": "<p></p>" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Storage buffer

pH: 7.3 Preservative: 0.02% Sodium azide Constituents: Tris buffered saline, 0.5% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Phosphodiesterase 4B (PDE4B) also known as PDE4B2 is an enzyme that specifically breaks down cyclic adenosine monophosphate (cAMP) to AMP an important process in cellular signaling. It belongs to the PDE4 family which is characterized by its ability to regulate the concentration of cAMP within cells. PDE4B has a molecular mass of approximately 75 kDa and is frequently expressed in immune cells including monocytes and neutrophils as well as in the brain.

Biological function summary

PDE4B plays a significant role in modulating intracellular signaling by controlling the levels of cAMP a second messenger involved in various cellular processes. As part of the larger PDE4 family complex PDE4B assists in fine-tuning immune responses and inflammatory processes. This enzyme modulates the activity of protein kinase A (PKA) impacting processes such as cell proliferation differentiation and apoptosis.

Pathways

PDE4B operates within the cAMP signaling pathway which is essential for transducing extracellular signals into cellular responses. This pathway involves interaction with other proteins such as PKA and CREB (cAMP response element-binding protein) which are critical for regulating gene expression and cellular functions. PDE4B's ability to modulate this pathway makes it fundamental in controlling the dynamics of cellular signaling.

PDE4B has been associated with inflammatory diseases such as asthma and chronic obstructive pulmonary disease (COPD). Its involvement in regulating cAMP levels affects the inflammatory response linking it to the pathogenesis of these conditions. Additionally PDE4B influences the activity of inflammatory mediators and cytokines contributing to disease progression and symptoms. For example PDE4 inhibitors can modulate these pathways presenting potential therapeutic opportunities for managing related inflammatory disorders.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Hydrolyzes the second messenger cAMP, which is a key regulator of many important physiological processes (PubMed : 15260978). May be involved in mediating central nervous system effects of therapeutic agents ranging from antidepressants to antiasthmatic and anti-inflammatory agents.

See full target information PDE4B

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Publications (3)

Recent publications for all applications. Explore the full list and refine your search

World journal of gastrointestinal oncology 16:1006-1018 PubMed38577450

2024

Ubiquitin-specific protease 21 promotes tumorigenicity and stemness of colorectal cancer by deubiquitinating and stabilizing ZEB1.

Applications

Unspecified application

Species

Unspecified reactive species

Jun-Jun Lin,Ye-Cai Lu

The Journal of physiology 594:6369-6393 PubMed27328735

2016

Differential impact of acute and prolonged cAMP agonist exposure on protein kinase A activation and human myometrium contractile activity.

Applications

Unspecified application

Species

Unspecified reactive species

Pei F Lai,Rachel M Tribe,Mark R Johnson

The Journal of biological chemistry 287:24941-54 PubMed22665489

2012

Transcriptional regulation of the protocadherin β cluster during Her-2 protein-induced mammary tumorigenesis results from altered N-glycan branching.

Applications

Unspecified application

Species

Unspecified reactive species

Huabei Guo,Alison Nairn,Mitche dela Rosa,Tamas Nagy,Shaying Zhao,Kelley Moremen,Michael Pierce

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-PDE4B antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PDE4B antibody (AB112014)

Western blot - Anti-PDE4B antibody (AB112014)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

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Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (3)

Product promise