Goat Polyclonal PDE4B3 antibody. Suitable for IHC-P, WB and reacts with Human, Mouse samples. Cited in 1 publication. Immunogen corresponding to Synthetic Peptide within Human PDE4B aa 700 to C-terminus.
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Publications
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- The Journal of physiology 594:6369-63932016Differential impact of acute and prolonged cAMP agonist exposure on protein kinase A activation and human myometrium contractile activity.Applications:Unspecified applicationReactive species:Unspecified reactive speciesPei F Lai et. al.PubMed 27328735
Key facts
- Isotype
- IgG
- Host species
- Goat
- Storage buffer
pH: 7.3
Preservative: 0.02% Sodium azide
Constituents: Tris buffered saline, 0.5% BSA- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- Synthetic Peptide within Human PDE4B aa 700 to C-terminus. The exact immunogen used to generate this antibody is proprietary information. Database link Q07343
Reactivity data
Select an application| IHC-P | WB | |
|---|---|---|
| Human | Tested | Expected |
| Mouse | Expected | Tested |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 5 µg/mL | Notes Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info Use at an assay dependent concentration. | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info 0.50000-1.00000 µg/mL | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info Use at an assay dependent concentration. | Notes - |
Associated Products
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3 products for Alternative Product
Target data
Function
Hydrolyzes the second messenger cAMP, which is a key regulator of many important physiological processes (PubMed:15260978). May be involved in mediating central nervous system effects of therapeutic agents ranging from antidepressants to antiasthmatic and anti-inflammatory agents.
Alternative names
DPDE4, PDE4B, PDE32, cAMP-specific phosphodiesterase 4B
Recommended products
Goat Polyclonal PDE4B3 antibody. Suitable for IHC-P, WB and reacts with Human, Mouse samples. Cited in 1 publication. Immunogen corresponding to Synthetic Peptide within Human PDE4B aa 700 to C-terminus.
Key facts
- Isotype
- IgG
- Host species
- Goat
- Storage buffer
pH: 7.3
Preservative: 0.02% Sodium azide
Constituents: Tris buffered saline, 0.5% BSA- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- Synthetic Peptide within Human PDE4B aa 700 to C-terminus. The exact immunogen used to generate this antibody is proprietary information. Database link Q07343
- Purification technique
- Affinity purification Immunogen
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
Phosphodiesterase 4B (PDE4B) also known as PDE4B2 is an enzyme that specifically breaks down cyclic adenosine monophosphate (cAMP) to AMP an important process in cellular signaling. It belongs to the PDE4 family which is characterized by its ability to regulate the concentration of cAMP within cells. PDE4B has a molecular mass of approximately 75 kDa and is frequently expressed in immune cells including monocytes and neutrophils as well as in the brain.
- Biological function summary
PDE4B plays a significant role in modulating intracellular signaling by controlling the levels of cAMP a second messenger involved in various cellular processes. As part of the larger PDE4 family complex PDE4B assists in fine-tuning immune responses and inflammatory processes. This enzyme modulates the activity of protein kinase A (PKA) impacting processes such as cell proliferation differentiation and apoptosis.
- Pathways
PDE4B operates within the cAMP signaling pathway which is essential for transducing extracellular signals into cellular responses. This pathway involves interaction with other proteins such as PKA and CREB (cAMP response element-binding protein) which are critical for regulating gene expression and cellular functions. PDE4B's ability to modulate this pathway makes it fundamental in controlling the dynamics of cellular signaling.
- Associated diseases and disorders
PDE4B has been associated with inflammatory diseases such as asthma and chronic obstructive pulmonary disease (COPD). Its involvement in regulating cAMP levels affects the inflammatory response linking it to the pathogenesis of these conditions. Additionally PDE4B influences the activity of inflammatory mediators and cytokines contributing to disease progression and symptoms. For example PDE4 inhibitors can modulate these pathways presenting potential therapeutic opportunities for managing related inflammatory disorders.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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2 product images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PDE4B antibody (ab112014)
ab112014, at 5µg/ml, staining PDE4B in formalin-fixed, paraffin-embedded Human Prostate tissue by Immunohistochemistry.
Western blot - Anti-PDE4B antibody (ab112014)
All lanes: Western blot - Anti-PDE4B antibody (ab112014) at 0.5 µg/mL
All lanes: Mouse Brain lysate (in RIPA buffer) at 35 µg
Developed using the ECL technique.
Predicted band size: 83 kDa
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