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AB97356

Anti-PDE4C antibody

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(1 Publication)

Rabbit Polyclonal PDE4C antibody. Suitable for WB and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Recombinant Fragment Protein within Human PDE4C aa 300 to C-terminus.

View Alternative Names

DPDE1, PDE4C, PDE21, cAMP-specific phosphodiesterase 4C

2 Images
Western blot - Anti-PDE4C antibody (AB97356)
  • WB

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Western blot - Anti-PDE4C antibody (AB97356)

7.5% SDS PAGE

All lanes:

Western blot - Anti-PDE4C antibody (ab97356) at 1/1000 dilution

All lanes:

293T whole cell lysate at 30 µg

Predicted band size: 80 kDa

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Western blot - Anti-PDE4C antibody (AB97356)
  • WB

CiteAb

Western blot - Anti-PDE4C antibody (AB97356)

PDE4C western blot using anti-PDE4C antibody ab97356. Publication image and figure legend from Fang, Q., Ma, Y., et al., 2013, Mediators Inflamm, PubMed 24227907.

ab97356 was used in this publication in western blot. This may not be the same as the application(s) guaranteed by Abcam. For a full list of applications guaranteed by Abcam for ab97356 please see the product overview.

Effect of PGE2 and indomethacin on PDE4 expression. Confluent HFL-1 cells were treated with SF-DMEM with or without 10-9 M PGE2 or 10-6 M indomethacin for 24 hrs. Whole cell lysates were immunoblotted for PDE4 isoforms and β-actin as loading control. Images were visualized with ECL plus (a), and the bands from 3 separate experiments were quantified (b). *p < 0.05.

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Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

WB

applications

Immunogen

Recombinant Fragment Protein within Human PDE4C aa 300 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

Q08493

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Primary Antibodies

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  • 0
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Primary Antibodies

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Anti-NR0B1 / Dax1 antibody [EP13786] - N-terminal

RabMAb|Recombinant|Trial Size

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  • 2
  • 3
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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "<p></p>" }, "Mouse": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
pH: 7 Preservative: 0.01% Thimerosal (merthiolate) Constituents: 10% Glycerol (glycerin, glycerine), 1.21% Tris, 0.75% Glycine
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

PDE4C also known as phosphodiesterase 4C is an enzyme that breaks down cyclic adenosine monophosphate (cAMP) to regulate its cellular levels. This target has a mass of approximately 80 kDa. PDE4C is expressed in various tissues including the brain lung and immune cells. It belongs to the larger group of PDE4 enzymes which share similar functions in hydrolyzing cAMP and are key in controlling cellular responses to extracellular signals.
Biological function summary

PDE4C modulates intracellular cAMP levels to influence various physiological functions. It does not function alone; rather it is a part of a regulatory complex that includes other PDE4 isoforms. This regulation of cAMP is important in processes like immune response mood regulation and cognitive function. The enzyme's role implies its involvement in controlling inflammation by modulating immune cell movement and activation.

Pathways

PDE4C plays significant roles in both the cAMP signaling pathway and the inflammatory response pathway. The cAMP pathway involves several proteins including adenylyl cyclase which synthesizes cAMP and protein kinase A (PKA) which is activated by cAMP. Through these interactions PDE4C influences signal transduction and regulatory mechanisms in cellular responses including those linked to inflammation and neural processes.

PDE4C associates with conditions such as asthma and depression. Its dysregulation can lead to abnormal inflammatory responses or altered neurotransmission. In asthma PDE4C affects airway reactivity and inflammation through interactions with other PDE4 enzymes such as PDE4A. In depression altered cAMP signaling involving PDE4C influences mood regulation and neuroplasticity highlighting its potential impact on psychological states and responses.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Hydrolyzes the second messenger cAMP, which is a key regulator of many important physiological processes.
See full target information PDE4C
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Mediators of inflammation 2013:145197 PubMed24227907

2013

PGE 2 desensitizes β -agonist effect on human lung fibroblast-mediated collagen gel contraction through upregulating PDE4.

Applications

Unspecified application

Species

Unspecified reactive species

Qiuhong Fang,Yingmin Ma,Jing Wang,Joel Michalski,Stephen I Rennard,Xiangde Liu
View all publications
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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