AB59212

Anti-PDE4D (phospho S190) antibody

Name: Anti-PDE4D (phospho S190) antibody (ab59212) | Abcam
Brand: Abcam Limited
SKU: AB59212

(1 Review)

(2 Publications)

Rabbit Polyclonal PDE4D phospho S190 antibody. Suitable for WB, IHC-P and reacts with Human samples. Cited in 2 publications. Immunogen corresponding to Synthetic Peptide within Human PDE4D phospho S190 aa 150-250.

View Alternative Names

DPDE3, PDE4D, PDE43, cAMP-specific phosphodiesterase 4D

2 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PDE4D (phospho S190) antibody (AB59212)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PDE4D (phospho S190) antibody (AB59212)

ab59212, at 1/50 dilution, staining PDE4D in paraffin embedded human colon carcinoma tissue by Immunohistochemistry in the absence or presence of the immunising peptide.

Unknown

Western blot - Anti-PDE4D (phospho S190) antibody (AB59212)

All lanes:

Western blot - Anti-PDE4D (phospho S190) antibody (ab59212) at 1/500 dilution

Lane 1:

K562 cell extract treatedwith H2O2 (100uM, 30mins)

Lane 2:

K562 cell extract treatedwith H2O2 (100uM, 30mins) with immunising peptide

Predicted band size: 91 kDa

Observed band size: 75 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Human

Applications

IHC-P, WB

applications

Immunogen

Synthetic Peptide within Human PDE4D phospho S190 aa 150-250. The exact immunogen used to generate this antibody is proprietary information.

Q08499

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/1000", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/50 - 1/100", "IHCP-species-notes": "" }, "Mouse": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Rat": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Purification notes

ab59212 was affinity purified from rabbit antiserum by affinity chromatography using epitope specific phosphopeptide. The antibody againstnon phosphopeptide was removed by chromatography using non phosphopeptide corresponding to the phosphorylation site.

Storage buffer

pH: 7.4 Preservative: 0.02% Sodium azide Constituents: PBS, 50% Glycerol (glycerin, glycerine), 0.87% Sodium chloride

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Storage information

Stable for 12 months at -20°C

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Phosphodiesterase 4D (PDE4D) is an enzyme that hydrolyzes cyclic AMP (cAMP) to its inactive form AMP controlling important cellular signaling pathways. It is part of the large PDE family and sometimes referred to as DPDE1. With a molecular weight of approximately 93 kDa PDE4D is widely expressed in the brain heart lungs and immune cells. Its expression in these tissues indicates an important regulatory role in both central and peripheral physiological processes.

Biological function summary

PDE4D plays an important role in the modulation of intracellular cAMP levels impacting processes such as inflammation cognition and cardiac function. It does not operate as part of a larger complex but collaborates with other signaling molecules to influence cellular responses. PDE4D's regulation of cAMP levels directly affects protein kinase A (PKA) leading to alterations in gene expression and cell activity.

Pathways

PDE4D integrates into the cAMP signaling and inflammatory pathways impacting various cellular responses. In the cAMP signaling pathway it partners with other PDEs and signaling proteins such as PKA which phosphorylates target proteins affecting cellular function. In inflammatory pathways PDE4D modulates immune cell activity by altering the cAMP-mediated inhibition of pro-inflammatory cytokine production linking it to inflammation-related responses.

PDE4D has associations with conditions such as asthma and ischemic stroke. In asthma PDE4D regulates cAMP levels in lung cells affecting bronchial constriction and inflammation. In ischemic stroke alterations in PDE4D activity influence vascular function by changing cAMP dynamics in vascular smooth muscle cells. Within these disorders PDE4D interacts with proteins like cytokines and COX-2 which also play parts in inflammatory pathways and vascular responses.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Hydrolyzes the second messenger cAMP, which is a key regulator of many important physiological processes.

See full target information PDE4D phospho S190

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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

Frontiers in systems neuroscience 10:5 PubMed26903822

2016

Traumatic Brain Injury Upregulates Phosphodiesterase Expression in the Hippocampus.

Applications

Species

Unspecified reactive species

Nicole M Wilson,David J Titus,Anthony A Oliva,Concepcion Furones,Coleen M Atkins

Journal of neurochemistry 123:1019-29 PubMed23057870

2012

Phosphodiesterase isoform-specific expression induced by traumatic brain injury.

Applications

Unspecified application

Species

Unspecified reactive species

Anthony A Oliva,Yuan Kang,Concepcion Furones,Ofelia F Alonso,Olga Bruno,W Dalton Dietrich,Coleen M Atkins

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-PDE4D (phospho S190) antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PDE4D (phospho S190) antibody (AB59212)

Western blot - Anti-PDE4D (phospho S190) antibody (AB59212)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Properties and storage information

Form

Purification technique

Purification notes

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (2)

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