Rabbit Polyclonal PDE4DIP antibody. Suitable for WB and reacts with Mouse, Rat, Human samples. Immunogen corresponding to Recombinant Fragment Protein within Human PDE4DIP aa 500-700.
Images
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2
Preservative: 0.02% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine)- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- Recombinant Fragment Protein within Human PDE4DIP aa 500-700. The exact immunogen used to generate this antibody is proprietary information. Database link Q5VU43
Reactivity data
Select an application| WB | |
|---|---|
| Human | Tested |
| Mouse | Tested |
| Rat | Tested |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info 0.04000-0.40000 µg/mL | Notes - |
Species Rat | Dilution info 0.04000-0.40000 µg/mL | Notes - |
Species Human | Dilution info 0.04000-0.40000 µg/mL | Notes - |
Target data
Function
Functions as an anchor sequestering components of the cAMP-dependent pathway to Golgi and/or centrosomes (By similarity). Isoform 13. Participates in microtubule dynamics, promoting microtubule assembly. Depending upon the cell context, may act at the level of the Golgi apparatus or that of the centrosome (PubMed:25217626, PubMed:27666745, PubMed:28814570, PubMed:29162697). In complex with AKAP9, recruits CAMSAP2 to the Golgi apparatus and tethers non-centrosomal minus-end microtubules to the Golgi, an important step for polarized cell movement (PubMed:27666745, PubMed:28814570). In complex with AKAP9, EB1/MAPRE1 and CDK5RAP2, contributes to microtubules nucleation and extension from the centrosome to the cell periphery, a crucial process for directed cell migration, mitotic spindle orientation and cell-cycle progression (PubMed:29162697).
Alternative names
CMYA2, KIAA0454, KIAA0477, MMGL, PDE4DIP, Myomegalin, Cardiomyopathy-associated protein 2, Phosphodiesterase 4D-interacting protein
Recommended products
Rabbit Polyclonal PDE4DIP antibody. Suitable for WB and reacts with Mouse, Rat, Human samples. Immunogen corresponding to Recombinant Fragment Protein within Human PDE4DIP aa 500-700.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2
Preservative: 0.02% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine)- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- Recombinant Fragment Protein within Human PDE4DIP aa 500-700. The exact immunogen used to generate this antibody is proprietary information. Database link Q5VU43
- Purification technique
- Affinity purification Immunogen
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.
If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.
Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
PDE4DIP also known as Myomegalin is a large protein with a molecular weight of approximately 260 kDa. This protein is mainly expressed in heart and brain tissues. PDE4DIP interacts with phosphodiesterase 4D targeting it to specific intracellular sites which is key for its mechanical functions. The interactions help maintain proper localization and function of phosphodiesterase 4D which plays a role in cAMP signaling processes.
- Biological function summary
The role of PDE4DIP extends to its involvement in cellular scaffolding. As part of the centrosomal complex it influences the organization of microtubules which affects cell division and intracellular transport. The presence of PDE4DIP in heart and brain tissues aligns with its biological functions where precise cellular organization is necessary for maintaining proper cellular responses and operations in these organs.
- Pathways
PDE4DIP’s involvement in cAMP signaling and microtubule dynamics positions it in key regulatory pathways. The protein interacts with phosphodiesterase 4D playing an important role in the modulation of cAMP levels which affects signaling pathways such as the PKA signaling pathway. Additionally the connection of PDE4DIP with microtubule-associated proteins situates it within the pathway governing cell structure and motility.
- Associated diseases and disorders
The aberrant expression or mutation of PDE4DIP has links to cardiac hypertrophy and neurodegenerative diseases. For cardiac hypertrophy the interaction with phosphodiesterase 4D influences signal pathways related to heart muscle cell growth. In neurodegenerative diseases such as Alzheimer's disease PDE4DIP’s role in microtubule dynamics and intracellular transport can affect neuronal structure and function possibly involving proteins connected to neurofibrillary tangles like tau protein.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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2 product images
Western blot - Anti-PDE4DIP antibody (ab121275)
All lanes: Western blot - Anti-PDE4DIP antibody (ab121275) at 0.4 µg/mL
Lane 1: U-251 MG (formally U-373 MG) (human brain glioma cell line) whole cell lysate
Lane 2: A549 (human lung carcinoma cell line) whole cell lysate
Predicted band size: 265 kDa
Western blot - Anti-PDE4DIP antibody (ab121275)
All lanes: Western blot - Anti-PDE4DIP antibody (ab121275) at 0.4 µg/mL
Lane 1: NIH/3T3 (mouse embryo fibroblast cell line) whole cell lysate
Lane 2: NBT-II (rat urinary bladder tumor cell line) whole cell lysate
Predicted band size: 265 kDa
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