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AB14672

Anti-PDE5A/PDE5 antibody

4

(1 Review)

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(15 Publications)

Rabbit Polyclonal PDE5A/PDE5 antibody. Suitable for IHC-P, IP, WB, ICC/IF and reacts with Human, Mouse, Rat samples. Cited in 15 publications. Immunogen corresponding to Synthetic Peptide within Human PDE5A.

View Alternative Names

PDE5, PDE5A, cGMP-binding cGMP-specific phosphodiesterase, CGB-PDE

2 Images
Immunocytochemistry/ Immunofluorescence - Anti-PDE5A/PDE5 antibody (AB14672)
  • ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-PDE5A/PDE5 antibody (AB14672)

ICC/IF image of ab14672 stained HeLa cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab14672, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PDE5A/PDE5 antibody (AB14672)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PDE5A/PDE5 antibody (AB14672)

ab14672 (4μg/ml) staining PDE5A/PDE5 in human oesophagus using an automated system (DAKO Autostainer Plus). Using this protocol there is staining of the cytoplasm and nuclei in stratified squamous epithelium cells.
Sections were rehydrated and antigen retrieved with the Dako 3 in 1 AR buffers EDTA pH 9.0 in a DAKO PT link. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 mins. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS) then incubated with primary antibody for 20 min and detected with Dako envision flex amplification kit for 30 minutes. Colorimetric detection was completed with Diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that, for manual staining, optimization of primary antibody concentration and incubation time is recommended. Signal amplification may be required.

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human, Mouse, Rat

Applications

IP, WB, IHC-P, ICC/IF

applications

Immunogen

Synthetic Peptide within Human PDE5A. The exact immunogen used to generate this antibody is proprietary information.

O76074

Specificity

This antibody detects 99 and 89 kDa PDE5A/PDE5 proteins in various tissues.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/10", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval before commencing with IHC staining protocol.", "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "<p></p>", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1 µg/mL", "ICCIF-species-notes": "<p></p>" }, "Mouse": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "<p></p>", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rat": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "<p></p>", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }
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Product details

Storage of very dilute antibody solutions is not recommended.
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Purification notes
Purified on antigen sepharose affinity column.
Storage buffer
Preservative: 0.02% Sodium azide
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

PDE5A also known as PDE5 or phosphodiesterase type 5 plays a critical role in hydrolyzing cyclic guanosine monophosphate (cGMP) to its inactive form. This enzyme is part of the phosphodiesterase family and has a molecular mass of approximately 100 kDa. PDE5A is expressed mainly in the smooth muscle cells of blood vessels and in the corpus cavernosum of the penis. Other expression sites include the lung heart and skeletal muscle.
Biological function summary

PDE5A regulates intracellular levels of cGMP an important secondary messenger in various signaling pathways. It is not part of a large protein complex but directly influences the contractility of smooth muscle tissues. By controlling cGMP levels PDE5A modulates vascular tone and influences processes such as vasodilation. This regulation is essential for maintaining a balance in the contractile state of muscles impacting blood flow and other vital functions.

Pathways

PDE5A contributes significantly to the nitric oxide (NO)-cGMP signaling pathway which is important for vascular relaxation. In this pathway nitric oxide stimulates guanylate cyclase to produce cGMP which then activates specific kinases and ion channels. Related proteins such as guanylate cyclase and cGMP-dependent protein kinases work alongside PDE5A to mediate smooth muscle relaxation and vasodilation particularly in penile erectile tissue.

PDE5A shows strong connections to erectile dysfunction and pulmonary hypertension. In the case of erectile dysfunction impaired regulation of cGMP by PDE5A affects the relaxation of smooth muscle leading to inadequate blood flow. Pulmonary hypertension involves elevated blood pressure in the lung arteries which PDE5A influences through its role in controlling vascular tone. The enzyme guanylate cyclase associates closely with PDE5A in these contexts as both influence cGMP pathways involved in these conditions.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Plays a role in signal transduction by regulating the intracellular concentration of cyclic nucleotides. This phosphodiesterase catalyzes the specific hydrolysis of cGMP to 5'-GMP (PubMed : 15489334, PubMed : 9714779). Specifically regulates nitric-oxide-generated cGMP (PubMed : 15489334).
See full target information PDE5A
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Publications (15)

Recent publications for all applications. Explore the full list and refine your search

Genesis (New York, N.Y. : 2000) 62:e23603 PubMed38738564

2024

Phosphodiesterase 5A regulates the vomeronasal pump in mice.

Applications

Unspecified application

Species

Unspecified reactive species

Dennean S Lippner,Jiang Xu,Siqi Ma,Johannes Reisert,Haiqing Zhao

Anti-cancer drugs 35:450-458 PubMed38452059

2024

A phase 1 study of regorafenib and sildenafil in adults with advanced solid tumors.

Applications

Unspecified application

Species

Unspecified reactive species

Andrew S Poklepovic,Sarah W Gordon,Sejal Kothadia,William P McGuire,Leroy R Thacker,Xiaoyan Deng,Mary Beth Tombes,Ellen Shrader,Daniel Hudson,Dipankar Bandyopadhyay,Alison A Ryan,Maciej Kmieciak,Steven Smith,Paul Dent

Respiratory research 23:87 PubMed35395852

2022

CRISPR-mediated Bmpr2 point mutation exacerbates late pulmonary vasculopathy and reduces survival in rats with experimental pulmonary hypertension.

Applications

Unspecified application

Species

Unspecified reactive species

Jane Chanda Kabwe,Hirofumi Sawada,Yoshihide Mitani,Hironori Oshita,Naoki Tsuboya,Erquan Zhang,Junko Maruyama,Yoshiki Miyasaka,Hideyoshi Ko,Kazunobu Oya,Hiromasa Ito,Noriko Yodoya,Shoichiro Otsuki,Hiroyuki Ohashi,Ryuji Okamoto,Kaoru Dohi,Yuhei Nishimura,Tomoji Mashimo,Masahiro Hirayama,Kazuo Maruyama

Oxidative medicine and cellular longevity 2021:5552530 PubMed34221234

2021

Pharmic Activation of PKG2 Alleviates Diabetes-Induced Osteoblast Dysfunction by Suppressing PLC1-Ca-Mediated Endoplasmic Reticulum Stress.

Applications

Unspecified application

Species

Unspecified reactive species

Tingting Jia,Ya-Nan Wang,Yao Feng,Chenchen Wang,Dongjiao Zhang,Xin Xu

Cancers 12: PubMed33322158

2020

Dual Pharmacological Targeting of HDACs and PDE5 Inhibits Liver Disease Progression in a Mouse Model of Biliary Inflammation and Fibrosis.

Applications

Unspecified application

Species

Unspecified reactive species

Alex Claveria-Cabello,Leticia Colyn,Iker Uriarte,Maria Ujue Latasa,Maria Arechederra,Jose M Herranz,Laura Alvarez,Jesus M Urman,Maria L Martinez-Chantar,Jesus M Banales,Bruno Sangro,Krista Rombouts,Julen Oyarzabal,Jose J G Marin,Carmen Berasain,Matias A Avila,Maite G Fernandez-Barrena

Biomedicine & pharmacotherapy = Biomedecine & phar 118:109216 PubMed31319371

2019

Cinaciguat in combination with insulin induces a favorable effect on implant osseointegration in type 2 diabetic rats.

Applications

Unspecified application

Species

Unspecified reactive species

Tingting Jia,Ya-Nan Wang,Jiajia Zhang,Xinyu Hao,Dongjiao Zhang,Xin Xu

Journal of the American Heart Association 8:e010961 PubMed31039672

2019

PI3Kα Pathway Inhibition With Doxorubicin Treatment Results in Distinct Biventricular Atrophy and Remodeling With Right Ventricular Dysfunction.

Applications

Unspecified application

Species

Unspecified reactive species

Brent A McLean,Vaibhav B Patel,Pavel Zhabyeyev,Xueyi Chen,Ratnadeep Basu,Faqi Wang,Saumya Shah,Bart Vanhaesebroeck,Gavin Y Oudit

The Prostate 79:909-919 PubMed30958912

2019

Activation of cGMP/PKG/p65 signaling associated with PDE5-Is downregulates CCL5 secretion by CD8 T cells in benign prostatic hyperplasia.

Applications

Unspecified application

Species

Unspecified reactive species

Song Jin,Peng Xiang,Jie Liu,Yang Yang,Shuai Hu,Jindong Sheng,Qun He,Wei Yu,Wenke Han,Jie Jin,Jing Peng

Scientific reports 9:4628 PubMed30874604

2019

Optimization of Extraction Technology of Majun Mupakhi Ela and its Effect on Hydrocortisone-induced Kidney Yang Deficiency in Mice.

Applications

Unspecified application

Species

Unspecified reactive species

Ayinuer Reheman,Ze-Yu Gao,Xirali Tursun,Xiao-Ping Pu,Tao Wu,Fei He,Xin Zhao,Haji Akber Aisa

PPAR research 2018:6970407 PubMed29686697

2018

PPAR Antagonizes Hypoxia-Induced Activation of Hepatic Stellate Cell through Cross Mediating PI3K/AKT and cGMP/PKG Signaling.

Applications

Unspecified application

Species

Unspecified reactive species

Qinghui Zhang,Shihao Xiang,Qingqian Liu,Tao Gu,Yongliang Yao,Xiaojie Lu
View all publications
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Product promise

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