AB5659

Anti-PDE6 alpha antibody

BOND RX™ Validated
What is this?

Be the first to review this product! Submit a review

(15 Publications)

Rabbit Polyclonal PDE6 alpha antibody. Suitable for IHC-P, WB and reacts with Human, Sheep samples. Cited in 15 publications. Immunogen corresponding to Synthetic Peptide within Human PDE6A aa 1-50.

View Alternative Names

PDEA, PDE6A, GMP-PDE alpha, PDE V-B1

2 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PDE6 alpha antibody (AB5659)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PDE6 alpha antibody (AB5659)

IHC image of ab5659 staining in human renal carcinoma formalin fixed paraffin embedded tissue section, performed on a Leica Bond^TM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab5659, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

Unknown

Western blot - Anti-PDE6 alpha antibody (AB5659)

Western blot of PDE6 alpha on sheep retinal extracts using ab5659.

All lanes:

Western blot - Anti-PDE6 alpha antibody (ab5659)

Predicted band size: 100 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Sheep, Human

Applications

WB, IHC-P

applications

Immunogen

Synthetic Peptide within Human PDE6A aa 1-50. The exact immunogen used to generate this antibody is proprietary information.

P16499

style

left-column

style

left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "5 µg/mL", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Dog": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Sheep": { "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1-2 µg/mL", "WB-species-notes": "<p>representing PDE6 alpha from sheep retinal extract. A higher molecular weight band is sometimes observed using sheep retinal extracts. See Guo et al, Kerov et al for details of WB procedure.</p>" } } }

style

left-column

style

left-column

Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Storage buffer

Preservative: 0.05% Sodium azide Constituents: PBS, 0.1% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

style

left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Phosphodiesterase 6 alpha (PDE6 alpha) is an enzyme that plays a significant role in the phototransduction pathway. It is also known as PDE6A and has a molecular mass of approximately 100 kDa. This enzyme is highly expressed in the rod and cone cells of the retina acting as a component in the conversion of light signals into visual information. PDE6 alpha hydrolyzes cyclic GMP into 5'-GMP decreasing the intracellular levels of cGMP. This action is critical in regulating the conductance of ion channels in photoreceptor cells.

Biological function summary

PDE6 alpha functions within a larger enzyme complex known as the PDE6 holoenzyme. This complex consists of multiple subunits including the PDE6 beta and PDE6 gamma subunits which form the catalytic core. PDE6 alpha contributes to the regulation of cGMP levels which is essential for maintaining the electrical response of photoreceptors to light. The precise action of hydrolyzing cGMP mediates the phototransduction cascade that eventually leads to visual perception.

Pathways

With regard to pathways in cellular function PDE6 alpha is an important player in the phototransduction and visual signal transduction pathways. Within the phototransduction pathway PDE6 alpha interacts closely with other proteins like rhodopsin and transducin. These proteins activate PDE6 alpha upon light exposure initiating the cascade that results in hyperpolarization of the photoreceptor cell membrane. The role of PDE6 alpha in managing cGMP concentration positions it as a major component in these pathways.

Mutations in the genes encoding PDE6 alpha have been associated with retinitis pigmentosa and congenital stationary night blindness. Retinitis pigmentosa a progressive degenerative disease of the retina often involves the dysfunction of photoreceptor cells where altered PDE6 alpha function impacts the phototransduction process. Additionally PDE6 alpha's involvement in these disorders also connects it to proteins such as arrestin and recoverin which further influence the progression of these visual impairments.

style

left-column

style

left-column

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

style

left-column

Target data

Rod-specific cGMP phosphodiesterase that catalyzes the hydrolysis of 3',5'-cyclic GMP (PubMed : 20940301). This protein participates in processes of transmission and amplification of the visual signal.

See full target information PDE6A

style

left-column

Publications (15)

Recent publications for all applications. Explore the full list and refine your search

The Journal of biological chemistry 301:108265 PubMed39909376

2025

Phosphodiesterase 5 expression in photoreceptors rescues retinal degeneration induced by deregulation of membrane guanylyl cyclase.

Applications

Unspecified application

Species

Unspecified reactive species

Alexander M Dizhoor,Shinya Sato,Zhuokai Luo,Lyuqi Tan,Fay E Levin,Elena V Olshevskaya,Igor V Peshenko,Vladimir J Kefalov

Scientific reports 12:20195 PubMed36424523

2022

Retinal Layer Separation (ReLayS) method enables the molecular analysis of photoreceptor segments and cell bodies, as well as the inner retina.

Applications

Unspecified application

Species

Unspecified reactive species

Vyara Todorova,Luca Merolla,Duygu Karademir,Gabriele M Wögenstein,Julian Behr,Lynn J A Ebner,Marijana Samardzija,Christian Grimm

Cellular and molecular life sciences : CMLS 78:6505-6532 PubMed34420069

2021

A look into retinal organoids: methods, analytical techniques, and applications.

Applications

Unspecified application

Species

Unspecified reactive species

Tess A V Afanasyeva,Julio C Corral-Serrano,Alejandro Garanto,Ronald Roepman,Michael E Cheetham,Rob W J Collin

The Journal of biological chemistry 291:10501-14 PubMed27008858

2016

Mfsd2a Is a Transporter for the Essential ω-3 Fatty Acid Docosahexaenoic Acid (DHA) in Eye and Is Important for Photoreceptor Cell Development.

Applications

Unspecified application

Species

Unspecified reactive species

Bernice H Wong,Jia Pei Chan,Amaury Cazenave-Gassiot,Rebecca W Poh,Juat Chin Foo,Dwight L A Galam,Sujoy Ghosh,Long N Nguyen,Veluchamy A Barathi,Sia W Yeo,Chi D Luu,Markus R Wenk,David L Silver

Cell communication and signaling : CCS 12:67 PubMed25323447

2014

Overexpression of rod photoreceptor glutamic acid rich protein 2 (GARP2) increases gain and slows recovery in mouse retina.

Applications

Species

Mouse

Shanta Sarfare,Alex S McKeown,Jeffrey Messinger,Glen Rubin,Hongjun Wei,Timothy W Kraft,Steven J Pittler

Nature communications 5:4047 PubMed24915161

2014

Generation of three-dimensional retinal tissue with functional photoreceptors from human iPSCs.

Applications

Unspecified application

Species

Unspecified reactive species

Xiufeng Zhong,Christian Gutierrez,Tian Xue,Christopher Hampton,M Natalia Vergara,Li-Hui Cao,Ann Peters,Tea Soon Park,Elias T Zambidis,Jason S Meyer,David M Gamm,King-Wai Yau,M Valeria Canto-Soler

Human molecular genetics 23:514-23 PubMed24101599

2013

Mid-stage intervention achieves similar efficacy as conventional early-stage treatment using gene therapy in a pre-clinical model of retinitis pigmentosa.

Applications

Species

Human

Katherine J Wert,Javier Sancho-Pelluz,Stephen H Tsang

Human molecular genetics 22:558-67 PubMed23108158

2012

Gene therapy provides long-term visual function in a pre-clinical model of retinitis pigmentosa.

Applications

Species

Mouse

Katherine J Wert,Richard J Davis,Javier Sancho-Pelluz,Patsy M Nishina,Stephen H Tsang

Proceedings of the National Academy of Sciences of 106:17534-9 PubMed19805139

2009

Retinoid-related orphan nuclear receptor RORbeta is an early-acting factor in rod photoreceptor development.

Applications

IHC-FoFr, WB

Species

Mouse, Mouse

Li Jia,Edwin C T Oh,Lily Ng,Maya Srinivas,Matthew Brooks,Anand Swaroop,Douglas Forrest

The Journal of neuroscience : the official journal of the Society for Neuroscience 29:9748-60 PubMed19657028

2009

Essential and synergistic roles of RP1 and RP1L1 in rod photoreceptor axoneme and retinitis pigmentosa.

Applications

Species

Mouse

Tetsuji Yamashita,Jiewu Liu,Jiangang Gao,Sean LeNoue,Changguan Wang,Jack Kaminoh,Sara J Bowne,Lori S Sullivan,Stephen P Daiger,Kang Zhang,Malinda E C Fitzgerald,Vladimir J Kefalov,Jian Zuo

View all publications

style

left-column

style

left-column

style

right-column

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-PDE6 alpha antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PDE6 alpha antibody (AB5659)

Western blot - Anti-PDE6 alpha antibody (AB5659)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Complementary Products

Secondary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (15)

Product promise