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AB236957

Anti-PDE6C antibody

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Rabbit Polyclonal PDE6C antibody. Suitable for ICC/IF and reacts with Human samples. Immunogen corresponding to Recombinant Fragment Protein within Human PDE6C aa 250-500.

View Alternative Names

PDEA2, PDE6C, cGMP phosphodiesterase 6C

1 Images
Immunocytochemistry/ Immunofluorescence - Anti-PDE6C antibody (AB236957)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-PDE6C antibody (AB236957)

A549 (human lung carcinoma cell line) cells labeling PDE6C (green) using ab236957 at 1/66 dilution in ICC/IF, followed by Alexa Fluor 488-conjugated goat anti-rabbit IgG (H+L). Counterstained with DAPI (blue).

The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal goat serum. Cells were then incubated with primary antibody overnight at 4°C.

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

ICC/IF

applications

Immunogen

Recombinant Fragment Protein within Human PDE6C aa 250-500. The exact immunogen used to generate this antibody is proprietary information.

P51160

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/50 - 1/200", "ICCIF-species-notes": "<p></p>" }, "Mouse": { "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Chicken": { "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Cow": { "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein G
Purification notes
Purity >95%.
Storage buffer
pH: 7.4 Preservative: 0.03% Proclin 300 Constituents: PBS, 50% Glycerol (glycerin, glycerine)
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Phosphodiesterase 6C (PDE6C) also known as cone phosphodiesterase is an important enzyme in the phototransduction pathway. It functions mechanically by hydrolyzing cyclic GMP (cGMP) into GMP a critical step in the visual signal transduction process. The PDE6C protein has a molecular mass of approximately 99 kDa. It is chiefly expressed in cone photoreceptor cells of the retina playing a pivotal role in converting light into electrical signals essential for vision.
Biological function summary

PDE6C plays a significant part in the visual process by regulating levels of cGMP in cone cells. It forms a critical component of the phototransduction cascade where it associates with other subunits including alpha beta and gamma subunits to form a functional enzyme complex. This complex adjusts cGMP levels in response to light exposure contributing to the adaptation of photoreceptors to varying light conditions helping modulate visual acuity and color differentiation.

Pathways

PDE6C is deeply embedded in the phototransduction pathway an important cascade for light perception. The hydrolysis of cGMP by PDE6C interacts with the cyclic nucleotide-gated (CNG) ion channels regulating the flow of ions and influencing the membrane potential of cone cells. It shows a relationship with other phototransduction proteins such as guanylate cyclase complementing the regulation of cGMP within the visual signaling pathway.

PDE6C mutations or malfunctions can lead to cone dystrophies and achromatopsia both impacting vision. These conditions result from disrupted cGMP levels in cone cells impairing normal phototransduction. Dysfunctions in PDE6C have indirect associations with GNAT2 another protein critical to phototransduction; this linkage influences the same pathway leading to compromised visual signal processing and associated retinal diseases.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

As cone-specific cGMP phosphodiesterase, it plays an essential role in light detection and cone phototransduction by rapidly decreasing intracellular levels of cGMP.
See full target information PDE6C
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions
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