Anti-PDE6D antibody
4
(1 Review)
|
(4 Publications)
Rabbit Polyclonal PDE6D antibody. Suitable for ICC, WB, ICC/IF, IHC-Fr and reacts with Mouse, Pig, Human, Dog, Cow, Sheep samples. Cited in 4 publications. Immunogen corresponding to Synthetic Peptide within Mouse Pde6d aa 1-50.
View Alternative Names
GMP-PDE delta, Pde6d
- ICC/IF
Supplier Data
Immunocytochemistry/ Immunofluorescence - Anti-PDE6D antibody (AB5665)
Immunocytochemistry/Immunofluorescence analysis of PDE6D (green) showing staining in the cytoplasm and nucleus of BAEC cells (right) compared to a negative control without primary antibody (left). Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were incubated with ab5665 in 3% BSA-PBS at a dilution of 1 : 100 and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight-conjugated secondary antibody in PBS at room temperature in the dark. F-actin (red) was stained with a fluorescent red phalloidin and nuclei (blue) were stained with Hoechst or DAPI. Images were taken at a magnification of 60x.
- ICC/IF
Supplier Data
Immunocytochemistry/ Immunofluorescence - Anti-PDE6D antibody (AB5665)
Immunocytochemistry/Immunofluorescence analysis of PDE6D (green) showing staining in the cytoplasm and nucleus of B-3 cells (right) compared to a negative control without primary antibody (left). Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were incubated with ab5565 in 3% BSA-PBS at a dilution of 1 : 100 and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight-conjugated secondary antibody in PBS at room temperature in the dark. F-actin (red) was stained with a fluorescent red phalloidin and nuclei (blue) were stained with Hoechst or DAPI. Images were taken at a magnification of 60x.
Reactivity data
Properties and storage information
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Supplementary information
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Biological function summary
PDE6D plays a role in the proper localization and function of various prenylated proteins contributing to retinal function. It is important for ciliary targeting allowing proteins like the small GTPase Ras and the retinitis pigmentosa protein RPGR to localize correctly. PDE6D itself does not form part of a larger complex but facilitates the interaction between proteins and other cell components ensuring that cellular processes proceed as needed.
Pathways
PDE6D functions as an important player in small GTPase-mediated signal transduction. This protein interacts with the Ras superfamily of small GTPases important for intracellular signaling pathways. It plays a role in processes such as cell growth differentiation and apoptosis. The proper functioning of PDE6D ensures that prenylated proteins like Ras which are also implicated in these pathways are effectively transported to their destinations within the cell.
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Target data
Publications (4)
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Proceedings of the National Academy of Sciences of the United States of America 120:e2212180120 PubMed36652482
2023
Applications
Unspecified application
Species
Unspecified reactive species
Biology open 13: PubMed34897417
2021
Applications
Unspecified application
Species
Unspecified reactive species
Journal of medicinal chemistry 62:2508-2520 PubMed30739444
2019
Applications
Unspecified application
Species
Unspecified reactive species
Stem cells international 2018:4968658 PubMed30008752
2018
Applications
Unspecified application
Species
Unspecified reactive species
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