Rabbit Polyclonal PDE6D antibody. Suitable for ICC, WB, ICC/IF, IHC-Fr and reacts with Mouse, Pig, Human, Dog, Cow, Sheep samples. Cited in 4 publications.
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- Proceedings of the National Academy of Sciences of the United States of America 120:e22121801202023Dimerization of the Alzheimer's disease pathogenic receptor SORLA regulates its association with retromer.Applications:Unspecified applicationReactive species:Unspecified reactive speciesAnne Mette G Jensen,Yu Kitago,Elnaz Fazeli,Christian B Vægter,Scott A Small,Gregory A Petsko,Olav M AndersenPubMed 36652482
- Biology open 13:2021The Abl-interactor Abi suppresses the function of the BRAG2 GEF family member Schizo.Applications:Unspecified applicationReactive species:Unspecified reactive speciesStefanie Lübke,Carina Braukmann,Karl-Heinz Rexer,Lubjinka Cigoja,Pratiti Rout,Susanne F ÖnelPubMed 34897417
- Journal of medicinal chemistry 62:2508-25202019Highly Selective PTK2 Proteolysis Targeting Chimeras to Probe Focal Adhesion Kinase Scaffolding Functions.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJohannes Popow et. al.PubMed 30739444
- Stem cells international 2018:49686582018Generation of Retinal Organoids with Mature Rods and Cones from Urine-Derived Human Induced Pluripotent Stem Cells.Applications:Unspecified applicationReactive species:Unspecified reactive speciesGuilan Li et. al.PubMed 30008752
Key facts
- Isotype
IgG
- Host species
Rabbit
- Storage buffer
Preservative: 0.05% Sodium azide
Constituents: 99% PBS, 0.1% BSA- Form
Liquid
- Clonality
Polyclonal
Reactivity data
Select an application| ICC | WB | ICC/IF | IHC-Fr | |
|---|---|---|---|---|
| Human | Expected | Expected | Predicted | Expected |
| Mouse | Expected | Expected | Tested | Expected |
| Cow | Expected | Expected | Tested | Expected |
| Dog | Expected | Expected | Predicted | Expected |
| Pig | Expected | Expected | Predicted | Expected |
| Sheep | Expected | Expected | Predicted | Expected |
ICC
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Pig, Human, Dog, Cow, Sheep | Dilution info Use at an assay dependent concentration. | Notes - |
WB
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Pig, Human, Dog, Cow, Sheep | Dilution info Use at an assay dependent concentration. | Notes - |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info 1/10.00000 - 1/200.00000 | Notes - |
Species Cow | Dilution info 1/10.00000 - 1/200.00000 | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Human, Pig, Sheep, Dog | Dilution info - | Notes - |
IHC-Fr
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info - | Notes Immunohistochemical staining of PDE6D in bovine retinal cross-sections using ab5665 results in staining of retinal outer segments. |
Species Pig | Dilution info - | Notes Immunohistochemical staining of PDE6D in bovine retinal cross-sections using ab5665 results in staining of retinal outer segments. |
Species Human | Dilution info - | Notes Immunohistochemical staining of PDE6D in bovine retinal cross-sections using ab5665 results in staining of retinal outer segments. |
Species Dog | Dilution info - | Notes Immunohistochemical staining of PDE6D in bovine retinal cross-sections using ab5665 results in staining of retinal outer segments. |
Species Cow | Dilution info - | Notes Immunohistochemical staining of PDE6D in bovine retinal cross-sections using ab5665 results in staining of retinal outer segments. |
Species Sheep | Dilution info - | Notes Immunohistochemical staining of PDE6D in bovine retinal cross-sections using ab5665 results in staining of retinal outer segments. |
Associated Products
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1 product for Alternative Product
Target data
Alternative names
GMP-PDE delta, Pde6d
Recommended products
Rabbit Polyclonal PDE6D antibody. Suitable for ICC, WB, ICC/IF, IHC-Fr and reacts with Mouse, Pig, Human, Dog, Cow, Sheep samples. Cited in 4 publications.
Key facts
- Isotype
IgG
- Host species
Rabbit
- Storage buffer
Preservative: 0.05% Sodium azide
Constituents: 99% PBS, 0.1% BSA- Form
Liquid
- Clonality
Polyclonal
- Purification technique
Affinity purification Immunogen
- Specificity
This antibody does not detect other PDE6 isoforms.
- Concentration
Storage
- Shipped at conditions
Blue Ice
- Appropriate short-term storage duration
1-2 weeks
- Appropriate short-term storage conditions
+4°C
- Appropriate long-term storage conditions
-20°C
- Aliquoting information
Upon delivery aliquot
- Storage information
Avoid freeze / thaw cycle
Notes
Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.
If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.
Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.
Supplementary info
This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
The PDE6D protein also known as Phosphodiesterase Delta or PDE6 delta serves a mechanical function as a prenyl-binding protein. It facilitates the release of prenylated proteins from cellular membranes allowing their proper trafficking within the cell. PDE6D has a molecular mass of approximately 17 kDa and is a part of the larger phosphodiesterase family. Expression of this target occurs mainly in retinal photoreceptor cells but is also found in several other tissues such as the brain and kidneys.
- Biological function summary
PDE6D plays a role in the proper localization and function of various prenylated proteins contributing to retinal function. It is important for ciliary targeting allowing proteins like the small GTPase Ras and the retinitis pigmentosa protein RPGR to localize correctly. PDE6D itself does not form part of a larger complex but facilitates the interaction between proteins and other cell components ensuring that cellular processes proceed as needed.
- Pathways
PDE6D functions as an important player in small GTPase-mediated signal transduction. This protein interacts with the Ras superfamily of small GTPases important for intracellular signaling pathways. It plays a role in processes such as cell growth differentiation and apoptosis. The proper functioning of PDE6D ensures that prenylated proteins like Ras which are also implicated in these pathways are effectively transported to their destinations within the cell.
- Associated diseases and disorders
PDE6D has been linked to retinitis pigmentosa a degenerative eye disease leading to retinal dysfunction. Mutations in the PDE6D gene disrupt the proper trafficking of prenylated proteins including RPGR which is essential for photoreceptor cell survival and function. Additionally PDE6D may also play a role in Joubert syndrome where it affects the localization of proteins necessary for proper ciliary function contributing to the disorders observed in this syndrome.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
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2 product images
Immunocytochemistry/ Immunofluorescence - Anti-PDE6D antibody (ab5665)
Immunocytochemistry/Immunofluorescence analysis of PDE6D (green) showing staining in the cytoplasm and nucleus of B-3 cells (right) compared to a negative control without primary antibody (left). Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were incubated with ab5565 in 3% BSA-PBS at a dilution of 1:100 and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight-conjugated secondary antibody in PBS at room temperature in the dark. F-actin (red) was stained with a fluorescent red phalloidin and nuclei (blue) were stained with Hoechst or DAPI. Images were taken at a magnification of 60x.
Immunocytochemistry/ Immunofluorescence - Anti-PDE6D antibody (ab5665)
Immunocytochemistry/Immunofluorescence analysis of PDE6D (green) showing staining in the cytoplasm and nucleus of BAEC cells (right) compared to a negative control without primary antibody (left). Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were incubated with ab5665 in 3% BSA-PBS at a dilution of 1:100 and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight-conjugated secondary antibody in PBS at room temperature in the dark. F-actin (red) was stained with a fluorescent red phalloidin and nuclei (blue) were stained with Hoechst or DAPI. Images were taken at a magnification of 60x.
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