Rabbit Polyclonal PDE6D antibody. Suitable for WB, IHC-P, ICC/IF and reacts with Mouse, Human samples. Cited in 1 publication. Immunogen corresponding to Recombinant Fragment Protein within Human PDE6D aa 1 to C-terminus.
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Publications
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- Acta pharmacologica Sinica 41:270-2772019EPHA2 feedback activation limits the response to PDEδ inhibition in KRAS-dependent cancer cells.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYue-Hong Chen,Hao Lv,Ning Shen,Xiao-Min Wang,Shuai Tang,Bing Xiong,Jian Ding,Mei-Yu Geng,Min HuangPubMed 31316177
Key facts
- Isotype
IgG
- Host species
Rabbit
- Storage buffer
pH: 7
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 10% Glycerol (glycerin, glycerine), 1.21% Tris, 0.75% Glycine- Form
Liquid
- Clonality
Polyclonal
Immunogen
- Recombinant Fragment Protein within Human PDE6D aa 1 to C-terminus. The exact immunogen used to generate this antibody is proprietary information. Database link O43924
Reactivity data
Select an application| WB | IHC-P | ICC/IF | |
|---|---|---|---|
| Human | Tested | Tested | Tested |
| Mouse | Tested | Expected | Expected |
| Rat | Predicted | Predicted | Predicted |
| Cow | Predicted | Predicted | Predicted |
| Dog | Predicted | Predicted | Predicted |
| Zebrafish | Predicted | Predicted | Predicted |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info 1/500.00000 - 1/5000.00000 | Notes - |
Species Human | Dilution info 1/500.00000 - 1/5000.00000 | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Rat, Dog, Zebrafish, Cow | Dilution info - | Notes - |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/100.00000 - 1/1000.00000 | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info Use at an assay dependent concentration. | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Rat, Dog, Zebrafish, Cow | Dilution info - | Notes - |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/100.00000 - 1/1000.00000 | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info Use at an assay dependent concentration. | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Rat, Dog, Zebrafish, Cow | Dilution info - | Notes - |
Associated Products
Select an associated product type
1 products for Alternative Product
Target data
Function
Promotes the release of prenylated target proteins from cellular membranes (PubMed:9712853). Modulates the activity of prenylated or palmitoylated Ras family members by regulating their subcellular location (PubMed:22002721, PubMed:23698361). Required for normal ciliary targeting of farnesylated target proteins, such as INPP5E (PubMed:24166846). Modulates the subcellular location of target proteins by acting as a GTP specific dissociation inhibitor (GDI) (By similarity). Increases the affinity of ARL3 for GTP by several orders of magnitude. Stabilizes ARL3-GTP by decreasing the nucleotide dissociation rate (By similarity).
Alternative names
GMP-PDE delta, Protein p17, PDE6D, PDED
Recommended products
Rabbit Polyclonal PDE6D antibody. Suitable for WB, IHC-P, ICC/IF and reacts with Mouse, Human samples. Cited in 1 publication. Immunogen corresponding to Recombinant Fragment Protein within Human PDE6D aa 1 to C-terminus.
Alternative names
GMP-PDE delta, Protein p17, PDE6D, PDED
Key facts
- Isotype
IgG
- Host species
Rabbit
- Storage buffer
pH: 7
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 10% Glycerol (glycerin, glycerine), 1.21% Tris, 0.75% Glycine- Form
Liquid
- Clonality
Polyclonal
- Immunogen
- Recombinant Fragment Protein within Human PDE6D aa 1 to C-terminus. The exact immunogen used to generate this antibody is proprietary information. Database link O43924
- Purification technique
Affinity purification Immunogen
- Concentration
Storage
- Shipped at conditions
Blue Ice
- Appropriate long-term storage conditions
-20°C
- Aliquoting information
Upon delivery aliquot
- Storage information
Avoid freeze / thaw cycle
Notes
Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.
If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.
Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.
Supplementary info
This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
The PDE6D protein also known as Phosphodiesterase Delta or PDE6 delta serves a mechanical function as a prenyl-binding protein. It facilitates the release of prenylated proteins from cellular membranes allowing their proper trafficking within the cell. PDE6D has a molecular mass of approximately 17 kDa and is a part of the larger phosphodiesterase family. Expression of this target occurs mainly in retinal photoreceptor cells but is also found in several other tissues such as the brain and kidneys.
- Biological function summary
PDE6D plays a role in the proper localization and function of various prenylated proteins contributing to retinal function. It is important for ciliary targeting allowing proteins like the small GTPase Ras and the retinitis pigmentosa protein RPGR to localize correctly. PDE6D itself does not form part of a larger complex but facilitates the interaction between proteins and other cell components ensuring that cellular processes proceed as needed.
- Pathways
PDE6D functions as an important player in small GTPase-mediated signal transduction. This protein interacts with the Ras superfamily of small GTPases important for intracellular signaling pathways. It plays a role in processes such as cell growth differentiation and apoptosis. The proper functioning of PDE6D ensures that prenylated proteins like Ras which are also implicated in these pathways are effectively transported to their destinations within the cell.
- Associated diseases and disorders
PDE6D has been linked to retinitis pigmentosa a degenerative eye disease leading to retinal dysfunction. Mutations in the PDE6D gene disrupt the proper trafficking of prenylated proteins including RPGR which is essential for photoreceptor cell survival and function. Additionally PDE6D may also play a role in Joubert syndrome where it affects the localization of proteins necessary for proper ciliary function contributing to the disorders observed in this syndrome.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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4 product images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PDE6D antibody (ab96825)
Paraffin-embedded SAS xenograft tissue labeling PDE6D with ab96825 at 1/500 dilution in immunohistochemical analysis.
Antigen retrieval: EDTA-based (pH 8.0), 15 minutes.
Immunocytochemistry/ Immunofluorescence - Anti-PDE6D antibody (ab96825)
Methanol-fixed HeLa (human epithelial cell line from cervix adenocarcinoma) cells labeling PDE6D with ab96825 at 1/500 dilution (green) in ICC/IF.
The nuclear counter stain is Hoechst 33342 (blue).
Western blot - Anti-PDE6D antibody (ab96825)
All lanes: Western blot - Anti-PDE6D antibody (ab96825) at 1/5000 dilution
All lanes: Mouse eye lysate at 50 µg
SecondaryAll lanes: Anti-Rabbit IgG HRP
Predicted band size: 17 kDa
Western blot - Anti-PDE6D antibody (ab96825)
12% SDS PAGE
All lanes: Western blot - Anti-PDE6D antibody (ab96825) at 1/1000 dilution
All lanes: MOLT4 whole cell lysate at 30 µg
Predicted band size: 17 kDa
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