AB96825

Anti-PDE6D antibody

Name: Anti-PDE6D antibody (ab96825) | Abcam
Brand: Abcam Limited
SKU: AB96825

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(3 Publications)

Rabbit Polyclonal PDE6D antibody. Suitable for WB, IHC-P, ICC/IF and reacts with Mouse, Human samples. Cited in 3 publications. Immunogen corresponding to Recombinant Fragment Protein within Human PDE6D aa 1 to C-terminus.

View Alternative Names

PDED, PDE6D, GMP-PDE delta, Protein p17

4 Images

Immunocytochemistry/ Immunofluorescence - Anti-PDE6D antibody (AB96825)

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-PDE6D antibody (AB96825)

Methanol-fixed HeLa (human epithelial cell line from cervix adenocarcinoma) cells labeling PDE6D with ab96825 at 1/500 dilution (green) in ICC/IF.

The nuclear counter stain is Hoechst 33342 (blue).

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PDE6D antibody (AB96825)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PDE6D antibody (AB96825)

Paraffin-embedded SAS xenograft tissue labeling PDE6D with ab96825 at 1/500 dilution in immunohistochemical analysis.

Antigen retrieval : EDTA-based (pH 8.0), 15 minutes.

Unknown

Western blot - Anti-PDE6D antibody (AB96825)

12% SDS PAGE

All lanes:

Western blot - Anti-PDE6D antibody (ab96825) at 1/1000 dilution

All lanes:

MOLT4 whole cell lysate at 30 µg

Predicted band size: 17 kDa

false

Supplier Data

Western blot - Anti-PDE6D antibody (AB96825)

All lanes:

Western blot - Anti-PDE6D antibody (ab96825) at 1/5000 dilution

All lanes:

Mouse eye lysate at 50 µg

Secondary

All lanes:

Anti-Rabbit IgG HRP

Predicted band size: 17 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Mouse, Human

Applications

IHC-P, WB, ICC/IF

applications

Immunogen

Recombinant Fragment Protein within Human PDE6D aa 1 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

O43924

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/5000", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/1000", "IHCP-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/100 - 1/1000", "ICCIF-species-notes": "" }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/5000", "WB-species-notes": "", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rat": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Cow": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Dog": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Zebrafish": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Storage buffer

pH: 7 Preservative: 0.01% Thimerosal (merthiolate) Constituents: 10% Glycerol (glycerin, glycerine), 1.21% Tris, 0.75% Glycine

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The PDE6D protein also known as Phosphodiesterase Delta or PDE6 delta serves a mechanical function as a prenyl-binding protein. It facilitates the release of prenylated proteins from cellular membranes allowing their proper trafficking within the cell. PDE6D has a molecular mass of approximately 17 kDa and is a part of the larger phosphodiesterase family. Expression of this target occurs mainly in retinal photoreceptor cells but is also found in several other tissues such as the brain and kidneys.

Biological function summary

PDE6D plays a role in the proper localization and function of various prenylated proteins contributing to retinal function. It is important for ciliary targeting allowing proteins like the small GTPase Ras and the retinitis pigmentosa protein RPGR to localize correctly. PDE6D itself does not form part of a larger complex but facilitates the interaction between proteins and other cell components ensuring that cellular processes proceed as needed.

Pathways

PDE6D functions as an important player in small GTPase-mediated signal transduction. This protein interacts with the Ras superfamily of small GTPases important for intracellular signaling pathways. It plays a role in processes such as cell growth differentiation and apoptosis. The proper functioning of PDE6D ensures that prenylated proteins like Ras which are also implicated in these pathways are effectively transported to their destinations within the cell.

PDE6D has been linked to retinitis pigmentosa a degenerative eye disease leading to retinal dysfunction. Mutations in the PDE6D gene disrupt the proper trafficking of prenylated proteins including RPGR which is essential for photoreceptor cell survival and function. Additionally PDE6D may also play a role in Joubert syndrome where it affects the localization of proteins necessary for proper ciliary function contributing to the disorders observed in this syndrome.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Promotes the release of prenylated target proteins from cellular membranes (PubMed : 9712853). Modulates the activity of prenylated or palmitoylated Ras family members by regulating their subcellular location (PubMed : 22002721, PubMed : 23698361). Required for normal ciliary targeting of farnesylated target proteins, such as INPP5E (PubMed : 24166846). Required for RAB28 localization to the cone cell outer segments in the retina (By similarity). Modulates the subcellular location of target proteins by acting as a GTP specific dissociation inhibitor (GDI) (By similarity). Increases the affinity of ARL3 for GTP by several orders of magnitude. Stabilizes ARL3-GTP by decreasing the nucleotide dissociation rate (By similarity).

See full target information PDE6D

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Publications (3)

Recent publications for all applications. Explore the full list and refine your search

Applied microbiology and biotechnology 109:1 PubMed39747723

2025

Targeted knockdown of ATM, ATR, and PDEδ increases Gag HIV-1 VLP production in HEK293 cells.

Applications

Unspecified application

Species

Unspecified reactive species

Andy Díaz-Maneh,Pol Pérez-Rubio,Cristina Rigau Granes,Laia Bosch-Molist,Jesús Lavado-García,Francesc Gòdia,Laura Cervera

World journal of surgical oncology 19:232 PubMed34362378

2021

Cyanidin-3-glucoside suppresses the progression of lung adenocarcinoma by downregulating TP53I3 and inhibiting PI3K/AKT/mTOR pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Xiaojun Chen,Weixia Zhang,Xiuzhen Xu

Acta pharmacologica Sinica 41:270-277 PubMed31316177

2019

EPHA2 feedback activation limits the response to PDEδ inhibition in KRAS-dependent cancer cells.

Applications

Unspecified application

Species

Unspecified reactive species

Yue-Hong Chen,Hao Lv,Ning Shen,Xiao-Min Wang,Shuai Tang,Bing Xiong,Jian Ding,Mei-Yu Geng,Min Huang

View all publications

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Product promise

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For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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Anti-PDE6D antibody

Immunocytochemistry/ Immunofluorescence - Anti-PDE6D antibody (AB96825)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PDE6D antibody (AB96825)

Western blot - Anti-PDE6D antibody (AB96825)

Western blot - Anti-PDE6D antibody (AB96825)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Alternative Products

Primary Antibodies

Complementary Products

Secondary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (3)

Product promise