AB313845

Anti-PDE6D antibody [HL1359] - BSA and Azide free

Recombinant
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Rabbit Recombinant Monoclonal PDE6D antibody. Carrier free. Suitable for WB, ICC/IF, IHC-P and reacts with Human, Mouse, Dog, Cat, Zebrafish samples. Immunogen corresponding to Recombinant Full Length Protein corresponding to Human PDE6D.

View Alternative Names

PDED, PDE6D, GMP-PDE delta, Protein p17, GMP-PDE delta, Pde6d

8 Images

Immunocytochemistry/ Immunofluorescence - Anti-PDE6D antibody [HL1359] - BSA and Azide free (AB313845)

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-PDE6D antibody [HL1359] - BSA and Azide free (AB313845)

Immunofluorescent analysis of MOLT-4 (Human lymphoblastic leukemia cell line) cells labeling PDE6D using ab313845 at a 1/500 dilution (green). Blue : Fluoroshield with DAPI.

Cells were fixed in 4% paraformaldehyde at RT for 15 mins.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PDE6D antibody [HL1359] - BSA and Azide free (AB313845)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PDE6D antibody [HL1359] - BSA and Azide free (AB313845)

Paraffin-embedded mouse eye tissue stained for PDE6D protein at cytoplasm with ab313845 at a 1/100 dilution in immunohistochemical analysis.

Antigen Retrieval : Citrate buffer, pH 6.0, 15 min.

Supplier Data

Western blot - Anti-PDE6D antibody [HL1359] - BSA and Azide free (AB313845)

All lanes:

Western blot - Anti-PDE6D antibody [HL1359] - BSA and Azide free (ab313845) at 1/1000 dilution

All lanes:

MOLT-4 (Human lymphoblastic leukemia cell line) whole cell extract at 30 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG antibody

Predicted band size: 17 kDa

true

Supplier Data

Western blot - Anti-PDE6D antibody [HL1359] - BSA and Azide free (AB313845)

All lanes:

Western blot - Anti-PDE6D antibody [HL1359] - BSA and Azide free (ab313845) at 1/1000 dilution

All lanes:

ARPE19 (Human retinal pigment epithelial cell line) whole cell extract at 30 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG antibody

Predicted band size: 17 kDa

true

Supplier Data

Western blot - Anti-PDE6D antibody [HL1359] - BSA and Azide free (AB313845)

All lanes:

Western blot - Anti-PDE6D antibody [HL1359] - BSA and Azide free (ab313845) at 1/1000 dilution

All lanes:

Mouse eye tissue extract at 50 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG antibody

Predicted band size: 17 kDa

true

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PDE6D antibody [HL1359] - BSA and Azide free (AB313845)

Paraffin-embedded cat colon tissue stained for PDE6D protein at cytoplasm with ab313845 at a 1/100 dilution in immunohistochemical analysis.

Antigen Retrieval : Citrate buffer, pH 6.0, 15 min.

Supplier Data

Western blot - Anti-PDE6D antibody [HL1359] - BSA and Azide free (AB313845)

Samples were separated by 15% SDS-PAGE.

All lanes:

Western blot - Anti-PDE6D antibody [HL1359] - BSA and Azide free (ab313845) at 1/1000 dilution

All lanes:

Whole zebrafish extract at 30 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG antibody

Predicted band size: 17 kDa

false

Supplier Data

Western blot - Anti-PDE6D antibody [HL1359] - BSA and Azide free (AB313845)

Samples were separated by 15% SDS-PAGE.

All lanes:

Western blot - Anti-PDE6D antibody [HL1359] - BSA and Azide free (ab313845) at 1/1000 dilution

Lane 1:

MDCK Whole cell extracts at 30 µg

Lane 2:

PG-4 whole cell extracts at 30 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG antibody

Predicted band size: 17 kDa

false

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

HL1359

Isotype

IgG

Carrier free

Yes

Reacts with

Human, Mouse, Cat, Dog, Zebrafish

Applications

IHC-P, ICC/IF, WB

applications

Immunogen

Recombinant Full Length Protein corresponding to Human PDE6D.

O43924

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/500", "ICCIF-species-notes": "", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Cat": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Dog": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Zebrafish": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }

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Product details

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.4 Constituents: PBS

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Aliquoting information

Upon delivery aliquot

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The PDE6D protein also known as Phosphodiesterase Delta or PDE6 delta serves a mechanical function as a prenyl-binding protein. It facilitates the release of prenylated proteins from cellular membranes allowing their proper trafficking within the cell. PDE6D has a molecular mass of approximately 17 kDa and is a part of the larger phosphodiesterase family. Expression of this target occurs mainly in retinal photoreceptor cells but is also found in several other tissues such as the brain and kidneys.

Biological function summary

PDE6D plays a role in the proper localization and function of various prenylated proteins contributing to retinal function. It is important for ciliary targeting allowing proteins like the small GTPase Ras and the retinitis pigmentosa protein RPGR to localize correctly. PDE6D itself does not form part of a larger complex but facilitates the interaction between proteins and other cell components ensuring that cellular processes proceed as needed.

Pathways

PDE6D functions as an important player in small GTPase-mediated signal transduction. This protein interacts with the Ras superfamily of small GTPases important for intracellular signaling pathways. It plays a role in processes such as cell growth differentiation and apoptosis. The proper functioning of PDE6D ensures that prenylated proteins like Ras which are also implicated in these pathways are effectively transported to their destinations within the cell.

PDE6D has been linked to retinitis pigmentosa a degenerative eye disease leading to retinal dysfunction. Mutations in the PDE6D gene disrupt the proper trafficking of prenylated proteins including RPGR which is essential for photoreceptor cell survival and function. Additionally PDE6D may also play a role in Joubert syndrome where it affects the localization of proteins necessary for proper ciliary function contributing to the disorders observed in this syndrome.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Promotes the release of prenylated target proteins from cellular membranes (PubMed : 9712853). Modulates the activity of prenylated or palmitoylated Ras family members by regulating their subcellular location (PubMed : 22002721, PubMed : 23698361). Required for normal ciliary targeting of farnesylated target proteins, such as INPP5E (PubMed : 24166846). Required for RAB28 localization to the cone cell outer segments in the retina (By similarity). Modulates the subcellular location of target proteins by acting as a GTP specific dissociation inhibitor (GDI) (By similarity). Increases the affinity of ARL3 for GTP by several orders of magnitude. Stabilizes ARL3-GTP by decreasing the nucleotide dissociation rate (By similarity).

See full target information PDE6D

Additional targets

Pde6d

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-PDE6D antibody [HL1359] - BSA and Azide free

Immunocytochemistry/ Immunofluorescence - Anti-PDE6D antibody [HL1359] - BSA and Azide free (AB313845)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PDE6D antibody [HL1359] - BSA and Azide free (AB313845)

Western blot - Anti-PDE6D antibody [HL1359] - BSA and Azide free (AB313845)

Western blot - Anti-PDE6D antibody [HL1359] - BSA and Azide free (AB313845)

Western blot - Anti-PDE6D antibody [HL1359] - BSA and Azide free (AB313845)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PDE6D antibody [HL1359] - BSA and Azide free (AB313845)

Western blot - Anti-PDE6D antibody [HL1359] - BSA and Azide free (AB313845)

Western blot - Anti-PDE6D antibody [HL1359] - BSA and Azide free (AB313845)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Complementary Products

Secondary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Additional targets

Product promise