AB154857

Anti-PDE7A/HCP1 antibody [EPR7731]

RabMAb
Recombinant
What is this?

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(5 Publications)

Rabbit Recombinant Monoclonal PDE7A/HCP1 antibody. Suitable for IHC-P, WB, ICC/IF and reacts with Human samples. Cited in 5 publications.

View Alternative Names

HCP1, TM22, cAMP-specific phosphodiesterase 7A, PDE7A

3 Images

Immunocytochemistry/ Immunofluorescence - Anti-PDE7A/HCP1 antibody [EPR7731] (AB154857)

ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-PDE7A/HCP1 antibody [EPR7731] (AB154857)

Immunofluorescent analysis of HuT78 cells labeling PDE7A/HCP1 with ab154857 at 1/250 dilution.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PDE7A/HCP1 antibody [EPR7731] (AB154857)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PDE7A/HCP1 antibody [EPR7731] (AB154857)

Immunohistochemical analysis of paraffin-embedded Human skeletal muscle tissue labeling PDE7A/HCP1 with ab154857 at 1/100 dilution.

Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

Unknown

Western blot - Anti-PDE7A/HCP1 antibody [EPR7731] (AB154857)

All lanes:

Western blot - Anti-PDE7A/HCP1 antibody [EPR7731] (ab154857) at 1/1000 dilution

All lanes:

HuT78 cell lysate at 10 µg

Secondary

All lanes:

Goat anti-rabbit HRP at 1/2000 dilution

Predicted band size: 56 kDa

false

Carrier free

Anti-PDE7A/HCP1 antibody [EPR7731] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR7731

Isotype

IgG

Carrier free

Reacts with

Human

Applications

ICC/IF, IHC-P, WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/250", "IHCP-species-notes": " Perform heat-mediated antigen retrieval before commencing with IHC staining protocol.", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/250 - 1/500", "ICCIF-species-notes": "" } } }

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Product details

Species reactivity
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species.
Please contact us for more information.

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

PDE7A also known as HCP1 is a phosphodiesterase enzyme responsible for breaking down cAMP an important signaling molecule in cells. This protein has a molecular weight of approximately 52 kDa. PDE7A is expressed in various tissues including muscles and immune cells. It plays a role in regulating cellular levels of cAMP which affects various physiological processes. HCP1 and HCP1 protein are common alternate labels used to refer to PDE7A.

Biological function summary

PDE7A influences immune response and muscle functions through its enzymatic activity on cAMP. The protein does not function alone but participates within larger protein complexes that modulate cAMP signaling. Its activity can affect immune cell activation offering an important checkpoint in the immune system. Additionally PDE7A's interaction with muscle cells highlights its role in controlling muscle contraction and energy metabolism.

Pathways

PDE7A has significant roles emphasizing the cAMP and immune signaling pathways. It helps control the levels of cAMP which is important for signal transduction. PDE7A interacts with proteins like PKA (Protein Kinase A) that rely on cAMP as a secondary messenger. Through this pathway PDE7A contributes to regulating processes such as inflammatory responses and metabolic pathways interlinking with other proteins like CREB which influences gene expression.

PDE7A's role connects it to conditions such as inflammatory diseases and certain muscular disorders. Abnormal PDE7A function can lead to dysregulation in inflammation implicating it in diseases such as rheumatoid arthritis. In the context of muscular disorders PDE7A levels affect muscle function and are related to conditions such as muscle dystrophy. It shares connections with TNF-alpha a protein involved in inflammation and immune system regulation further linking PDE7A to these disorders.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Hydrolyzes the second messenger cAMP, which is a key regulator of many important physiological processes (PubMed : 19350606, PubMed : 8389765, PubMed : 9195912). May have a role in muscle signal transduction (PubMed : 9195912).

See full target information PDE7A

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Publications (5)

Recent publications for all applications. Explore the full list and refine your search

Cell reports. Medicine 6:102356 PubMed40961924

2025

PDE7A inhibition suppresses triple-negative breast cancer by attenuating de novo pyrimidine biosynthesis.

Applications

Unspecified application

Species

Unspecified reactive species

Parmanand Malvi,Suresh Bugide,Roshan Dutta,Kiran Kumar Reddi,Yvonne J K Edwards,Kamaljeet Singh,Romi Gupta,Narendra Wajapeyee

Cancer research 81:2470-2480 PubMed33685991

2021

Genomic Alterations in -Mutated Breast Cancer Result in mTORC1 Activation and Limit the Sensitivity to PI3Kα Inhibitors.

Applications

Unspecified application

Species

Unspecified reactive species

Yanyan Cai,Guotai Xu,Fan Wu,Flavia Michelini,Carmen Chan,Xuan Qu,Pier Selenica,Erik Ladewig,Pau Castel,Yuanming Cheng,Alison Zhao,Komal Jhaveri,Eneda Toska,Marta Jimenez,Alexandra Jacquet,Alicia Tran-Dien,Fabrice Andre,Sarat Chandarlapaty,Jorge S Reis-Filho,Pedram Razavi,Maurizio Scaltriti

Archives of biochemistry and biophysics 662:160-168 PubMed30550727

2018

Phosphodiesterase expression in the normal and failing heart.

Applications

Unspecified application

Species

Unspecified reactive species

Edwin A Li,Wang Xi,Young Soo Han,Frank V Brozovich

International journal of clinical and experimental 10:9436-9443 PubMed31966816

2017

MiR-23b inhibits cell migration and invasion through targeting PDE7A in colon cancer cells.

Applications

Unspecified application

Species

Unspecified reactive species

Lingfang Hao,Honggang Yu

International journal of oncology 47:325-34 PubMed25955017

2015

The tumor-suppressive microRNA-1/133a cluster targets PDE7A and inhibits cancer cell migration and invasion in endometrial cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Noriko Yamamoto,Rika Nishikawa,Takeshi Chiyomaru,Yusuke Goto,Ichiro Fukumoto,Hirokazu Usui,Akira Mitsuhashi,Hideki Enokida,Masayuki Nakagawa,Makio Shozu,Naohiko Seki

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-PDE7A/HCP1 antibody [EPR7731]

Immunocytochemistry/ Immunofluorescence - Anti-PDE7A/HCP1 antibody [EPR7731] (AB154857)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PDE7A/HCP1 antibody [EPR7731] (AB154857)

Western blot - Anti-PDE7A/HCP1 antibody [EPR7731] (AB154857)

Related conjugates and formulations (1)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Complementary Products

Assay Kits

Secondary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (5)

Product promise