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AB170914

Anti-PDE7B antibody [EPR11491]

5

(1 Review)

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(3 Publications)

Rabbit Recombinant Monoclonal PDE7B antibody. Suitable for IHC-P, WB, ICC/IF, Flow Cyt (Intra) and reacts with Human samples. Cited in 3 publications.

View Alternative Names

cAMP-specific phosphodiesterase 7B, PDE7B

5 Images
Flow Cytometry (Intracellular) - Anti-PDE7B antibody [EPR11491] (AB170914)
  • Flow Cyt (Intra)

Supplier Data

Flow Cytometry (Intracellular) - Anti-PDE7B antibody [EPR11491] (AB170914)

Intracellular flow cytometric analysis of permeabilized Hela cells labeling PDE7Busing ab170914 at 1/500 dilution (red) or a rabbit IgG negative (green).

Immunocytochemistry/ Immunofluorescence - Anti-PDE7B antibody [EPR11491] (AB170914)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-PDE7B antibody [EPR11491] (AB170914)

Immunofluorescent analysis of HeLa cells labeling PDE7B using ab170914 at 1/500 dilution. DAPI nuclear staining (blue).

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PDE7B antibody [EPR11491] (AB170914)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PDE7B antibody [EPR11491] (AB170914)

Immunohistochemical analysis of paraffin-embedded Human heart tissue labeling PDE7B with ab170914 at 1/100 dilution

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PDE7B antibody [EPR11491] (AB170914)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PDE7B antibody [EPR11491] (AB170914)

Immunohistochemical analysis of paraffin-embedded Human brain tissue labeling PDE7B with ab170914 at 1/100 dilution

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Western blot - Anti-PDE7B antibody [EPR11491] (AB170914)
  • WB

Supplier Data

Western blot - Anti-PDE7B antibody [EPR11491] (AB170914)

All lanes:

Western blot - Anti-PDE7B antibody [EPR11491] (ab170914) at 1/1000 dilution

Lane 1:

MCF-7 cell lysate at 10 µg

Lane 2:

HeLa cell lysate at 10 µg

Lane 3:

HepG2 cell lysate at 10 µg

Lane 4:

BxPC-3 cell lysate at 10 µg

Lane 5:

Human fetal brain tissue lysate at 10 µg

Secondary

All lanes:

Goat anti-rabbit HRP at 1/2000 dilution

Predicted band size: 52 kDa

false

  • Carrier free

    Anti-PDE7B antibody [EPR11491] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR11491

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

IHC-P, ICC/IF, Flow Cyt (Intra), WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/250 - 1/500", "IHCP-species-notes": "<p></p>", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/5000", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/100 - 1/250", "ICCIF-species-notes": "<p></p>", "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "1/500 - 1/1000", "FlowCytIntra-species-notes": "<p><a href='/en-us/products/primary-antibodies/rabbit-igg-monoclonal-epr25a-isotype-control-ab172730'>ab172730</a> - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.</p>" }, "Mouse": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "predicted", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" }, "Rat": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "predicted", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" } } }
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Product details

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 50% Tissue culture supernatant, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Phosphodiesterase 7B (PDE7B) is an enzyme that plays an important role in the hydrolysis of cyclic adenosine monophosphate (cAMP) a critical second messenger in cellular signaling. Also known as HCP1 PDE7B has a molecular mass of approximately 49 kDa. It is expressed in various tissues throughout the body with marked expression in the brain particularly in regions associated with cognition and motor function. This expression pattern suggests that PDE7B may have specialized roles in neural activities and processes.
Biological function summary

The involvement of PDE7B in the hydrolysis of cAMP points to its role in signal transduction pathways that regulate physiological responses. By degrading cAMP PDE7B modulates signal strength and duration influencing cellular responses such as proliferation differentiation and survival. While there is no evidence PDE7B forms part of a larger protein complex its action interacts with multiple signaling cascades making it an integral part of cellular response machinery.

Pathways

The functions of PDE7B are integral to cAMP-specific signaling pathways such as the PKA (protein kinase A) signaling cascade. By regulating cAMP levels PDE7B indirectly influences the activity of PKA which phosphorylates various target proteins to execute cellular functions. PDE7B is closely related to other phosphodiesterases like PDE4 and PDE1 in these pathways which also target cAMP but have different expression and activity regulation.

PDE7B presents potential involvement in neurological and inflammatory conditions. Abnormal PDE7B function or expression could contribute to disorders such as Parkinson's disease where altered signaling may impact neuronal survival and dopamine regulation. Moreover by modulating cAMP levels PDE7B might also associate with chronic inflammatory disorders wherein dysregulated immune responses play an important role. Research into PDE7B's interactions with other proteins like PDE4 could uncover new therapeutic strategies for managing these conditions.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Hydrolyzes the second messenger cAMP, which is a key regulator of many important physiological processes (PubMed : 10814504, PubMed : 10872825). May be involved in the control of cAMP-mediated neural activity and cAMP metabolism in the brain (PubMed : 10814504).
See full target information PDE7B
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Publications (3)

Recent publications for all applications. Explore the full list and refine your search

The international journal of neuropsychopharmacology 27: PubMed39099166

2024

Identification of Phosphodiesterase-7A (PDE7A) as a Novel Target for Reducing Ethanol Consumption in Mice.

Applications

Unspecified application

Species

Unspecified reactive species

Ran Wei,Fangjiao Zong,Jiahao Dong,Wei Zhao,Fangfang Zhang,Wei Wang,Shuang Zhao,Ziqi Wang,Fang Zhang,Han-Ting Zhang

Nature communications 13:7815 PubMed36535970

2022

Targeting the transcription factor HES1 by L-menthol restores protein phosphatase 6 in keratinocytes in models of psoriasis.

Applications

Unspecified application

Species

Unspecified reactive species

Zhikai Wang,Yang Sun,Fangzhou Lou,Jing Bai,Hong Zhou,Xiaojie Cai,Libo Sun,Qianqian Yin,Sibei Tang,Yue Wu,Li Fan,Zhenyao Xu,Hong Wang,Xiaoyu Hu,Honglin Wang

Evidence-based complementary and alternative medic 2020:3587095 PubMed32922506

2020

Ethyl Acetate Fraction from plus Exerts Anti-Breast Cancer Effect via miR-200c-PDE7B/PD-L1-AKT/MAPK Axis.

Applications

Unspecified application

Species

Unspecified reactive species

Yue Yang,Ting Fang,Yi-Lan Cao,Ya-Xin Lv,Qing-Qi Chang,Dan-Dan Zhang
View all publications
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