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AB249586

Anti-PDE7B antibody [EPR11491] - BSA and Azide free

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Rabbit Recombinant Monoclonal PDE7B antibody. Carrier free. Suitable for IHC-P, ICC/IF, WB, Flow Cyt (Intra) and reacts with Human samples.

View Alternative Names

cAMP-specific phosphodiesterase 7B, PDE7B

5 Images
Flow Cytometry (Intracellular) - Anti-PDE7B antibody [EPR11491] - BSA and Azide free (AB249586)
  • Flow Cyt (Intra)

Supplier Data

Flow Cytometry (Intracellular) - Anti-PDE7B antibody [EPR11491] - BSA and Azide free (AB249586)

This data was developed using ab170914, the same antibody clone in a different buffer formulation.

Intracellular flow cytometric analysis of permeabilized Hela cells labeling PDE7B using ab170914 at 1/500 dilution (red) or a rabbit IgG negative (green).

Immunocytochemistry/ Immunofluorescence - Anti-PDE7B antibody [EPR11491] - BSA and Azide free (AB249586)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-PDE7B antibody [EPR11491] - BSA and Azide free (AB249586)

This data was developed using ab170914, the same antibody clone in a different buffer formulation.

Immunofluorescent analysis of HeLa cells labeling PDE7B using ab170914 at 1/500 dilution. DAPI nuclear staining (blue).

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PDE7B antibody [EPR11491] - BSA and Azide free (AB249586)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PDE7B antibody [EPR11491] - BSA and Azide free (AB249586)

This data was developed using ab170914, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin-embedded Human heart tissue labeling PDE7B with ab170914 at 1/100 dilution

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PDE7B antibody [EPR11491] - BSA and Azide free (AB249586)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PDE7B antibody [EPR11491] - BSA and Azide free (AB249586)

This data was developed using ab170914, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin-embedded Human brain tissue labeling PDE7B with ab170914 at 1/100 dilution

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Western blot - Anti-PDE7B antibody [EPR11491] - BSA and Azide free (AB249586)
  • WB

Supplier Data

Western blot - Anti-PDE7B antibody [EPR11491] - BSA and Azide free (AB249586)

This data was developed using ab170914, the same antibody clone in a different buffer formulation.

All lanes:

Western blot - Anti-PDE7B antibody [EPR11491] (<a href='/en-us/products/primary-antibodies/pde7b-antibody-epr11491-ab170914'>ab170914</a>) at 1/1000 dilution

Lane 1:

MCF-7 cell lysate at 10 µg

Lane 2:

HeLa cell lysate at 10 µg

Lane 3:

HepG2 cell lysate at 10 µg

Lane 4:

BxPC-3 cell lysate at 10 µg

Lane 5:

Human fetal brain tissue lysate at 10 µg

Secondary

All lanes:

Goat anti-rabbit HRP at 1/2000 dilution

Predicted band size: 52 kDa

false

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR11491

Isotype

IgG

Carrier free

Yes

Reacts with

Human

Applications

IHC-P, WB, Flow Cyt (Intra), ICC/IF

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p>Antigen retrieval is recommended.</p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>", "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "<p></p>" }, "Mouse": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "FlowCytIntra-species-checked": "predicted", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" }, "Rat": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "FlowCytIntra-species-checked": "predicted", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" } } }
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Product details

ab249586 is the carrier-free version of ab170914.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 - 7.4 Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
Do Not Freeze
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Phosphodiesterase 7B (PDE7B) is an enzyme that plays an important role in the hydrolysis of cyclic adenosine monophosphate (cAMP) a critical second messenger in cellular signaling. Also known as HCP1 PDE7B has a molecular mass of approximately 49 kDa. It is expressed in various tissues throughout the body with marked expression in the brain particularly in regions associated with cognition and motor function. This expression pattern suggests that PDE7B may have specialized roles in neural activities and processes.
Biological function summary

The involvement of PDE7B in the hydrolysis of cAMP points to its role in signal transduction pathways that regulate physiological responses. By degrading cAMP PDE7B modulates signal strength and duration influencing cellular responses such as proliferation differentiation and survival. While there is no evidence PDE7B forms part of a larger protein complex its action interacts with multiple signaling cascades making it an integral part of cellular response machinery.

Pathways

The functions of PDE7B are integral to cAMP-specific signaling pathways such as the PKA (protein kinase A) signaling cascade. By regulating cAMP levels PDE7B indirectly influences the activity of PKA which phosphorylates various target proteins to execute cellular functions. PDE7B is closely related to other phosphodiesterases like PDE4 and PDE1 in these pathways which also target cAMP but have different expression and activity regulation.

PDE7B presents potential involvement in neurological and inflammatory conditions. Abnormal PDE7B function or expression could contribute to disorders such as Parkinson's disease where altered signaling may impact neuronal survival and dopamine regulation. Moreover by modulating cAMP levels PDE7B might also associate with chronic inflammatory disorders wherein dysregulated immune responses play an important role. Research into PDE7B's interactions with other proteins like PDE4 could uncover new therapeutic strategies for managing these conditions.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Hydrolyzes the second messenger cAMP, which is a key regulator of many important physiological processes (PubMed : 10814504, PubMed : 10872825). May be involved in the control of cAMP-mediated neural activity and cAMP metabolism in the brain (PubMed : 10814504).
See full target information PDE7B
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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