AB109597

Anti-PDE8A antibody

Name: Anti-PDE8A antibody (ab109597) | Abcam
Brand: Abcam Limited
SKU: AB109597

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(4 Publications)

Rabbit Polyclonal PDE8A antibody. Suitable for IP, WB and reacts with Human samples. Cited in 4 publications. Immunogen corresponding to Synthetic Peptide within Human PDE8A.

View Alternative Names

PDE8A

2 Images

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Immunoprecipitation - Anti-PDE8A antibody (AB109597)

Detection of PDE8A by Western Blot of Immunprecipitate.
Lane 1 : ab109597 at 1µg/ml staining PDE8A in HeLa whole cell lysate immunoprecipitated using ab109597 at 6µg/mg lysate (1 mg/IP; 20% of IP loaded/lane).
Lane 2 : Control IgG
Detection : Chemiluminescence with exposure time of 30 seconds.

All lanes:

Immunoprecipitation - Anti-PDE8A antibody (ab109597) at 1 µg

Lane 1:

IP of 6 µg of PDE8A antibody/mg HeLa cell lysate at 1000 µg

Lane 2:

Control IgG with HeLa cell lysate at 1000 µg

Predicted band size: 93 kDa

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Western blot - Anti-PDE8A antibody (AB109597)

All lanes:

Western blot - Anti-PDE8A antibody (ab109597) at 0.1 µg/mL

Lane 1:

HeLa cell lysate at 50 µg

Lane 2:

HeLa cell lysate at 15 µg

Lane 3:

HeLa cell lysate at 5 µg

Lane 4:

293T cell lysate at 50 µg

Predicted band size: 93 kDa

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Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Human

Applications

WB, IP

applications

Immunogen

Synthetic Peptide within Human PDE8A. The exact immunogen used to generate this antibody is proprietary information.

O60658

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "2-5 µg/mg of lysate", "IP-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/2000 - 1/10000", "WB-species-notes": "<p></p>" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Storage buffer

pH: 7 - 8 Preservative: 0.09% Sodium azide Constituents: Tris citrate/phosphate

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The protein PDE8A also known as phosphodiesterase 8A performs as an enzyme that hydrolyzes cyclic adenosine monophosphate (cAMP) to adenosine monophosphate (AMP). This action regulates the intracellular levels of cAMP an important second messenger in many signaling pathways. PDE8A has a molecular mass of approximately 100 kDa and is expressed mainly in endocrine tissues like the adrenal gland and thyroid as well as in specific immune cells.

Biological function summary

The regulatory function of PDE8A on cAMP levels impacts many processes including hormone secretion and immune responses. It often functions independently rather than as part of a complex but sometimes may interface with other proteins mediating these pathways. By controlling cAMP PDE8A influences various biological actions like glucose metabolism and T-cell activation.

Pathways

PDE8A activity connects to key signaling pathways such as the cAMP-dependent protein kinase (PKA) pathway where it modulates responses through cAMP degradation. In this pathway it interacts indirectly with proteins like CREB (cAMP response element-binding protein) affecting transcriptional activity that governs metabolic and immune responses. PDE8A also interfaces with other phosphodiesterases which further modulate the cAMP levels in the cell.

Alterations in PDE8A levels or function have links to adrenal hyperplasia where abnormal hormone production affects body processes. PDE8A and its regulatory role in cAMP balance also relate to immune function disorders as improper T-cell activation leads to autoimmune conditions. These associations illustrate PDE8A's involvement with broader protein interactions including those regulating endocrine and immune homeostasis.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
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Target data

Hydrolyzes the second messenger cAMP, which is a key regulator of many important physiological processes (PubMed : 18983167). May be involved in maintaining basal levels of the cyclic nucleotide and/or in the cAMP regulation of germ cell development (PubMed : 18983167). Binding to RAF1 reduces RAF1 'Ser-259' inhibitory-phosphorylation and stimulates RAF1-dependent EGF-activated ERK-signaling (PubMed : 23509299). Protects against cell death induced by hydrogen peroxide and staurosporine (PubMed : 23509299).

See full target information PDE8A

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Publications (4)

Recent publications for all applications. Explore the full list and refine your search

The international journal of neuropsychopharmacology 28: PubMed40312965

2025

Cognitive improvement effects of PF-04957325, a phosphodiesterase-8 inhibitor, in mouse models of Alzheimer's disease via modulating neuroinflammation.

Applications

Unspecified application

Species

Unspecified reactive species

Tian-Yang Guo,Meng Zhang,Yu-Li Lv,Nian-Zhuang Qiu,Rui-Min Chen,Fang-Fang Zhang,Wei Chen,Feng Zhang,Yong-Feng Gao,Xiao-Dan Wang,Xue-Hui Zhang,Mei-Hua Chen,Han-Ting Zhang,Hao Wang

Molecules and cells 47:100148 PubMed39537030

2024

E2F3a activates Gadd45b gene expression through PPARα-mediated transcriptional regulation in hepatic cells.

Applications

Unspecified application

Species

Unspecified reactive species

Min Seok Woo,Atif Ali Khan Khalil,Frank J Gonzalez,Jung-Hwan Kim

Cellular physiology and biochemistry : international journal of experimental cellular physiology, biochemistry, and pharmacology 55:784-804 PubMed34936285

2021

Phosphodiesterase 8A Regulates CFTR Activity in Airway Epithelial Cells.

Applications

Unspecified application

Species

Unspecified reactive species

Mark J Turner,Yukiko Sato,David Y Thomas,Kathy Abbott-Banner,John W Hanrahan

Journal of cellular and molecular medicine 25:3460-3468 PubMed33713531

2021

H S catalysed by CBS regulates testosterone synthesis through affecting the sulfhydrylation of PDE.

Applications

Unspecified application

Species

Unspecified reactive species

Jing Wang,Jing Wang,Tao Shen,Renyun Hong,Shanshan Tang,Xia Zhao

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-PDE8A antibody

Immunoprecipitation - Anti-PDE8A antibody (AB109597)

Western blot - Anti-PDE8A antibody (AB109597)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Complementary Products

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (4)

Product promise