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AB112024

Anti-PDE8B antibody

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(1 Publication)

Rabbit Polyclonal PDE8B antibody. Suitable for IP, WB and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Synthetic Peptide within Human PDE8B aa 800 to C-terminus.

View Alternative Names

PIG22, PDE8B, HsPDE8B, Cell proliferation-inducing gene 22 protein

2 Images
Immunoprecipitation - Anti-PDE8B antibody (AB112024)
  • IP

Unknown

Immunoprecipitation - Anti-PDE8B antibody (AB112024)

Immunoprecipitation of PDE8B. Samples : Whole cell lysate from HeLa cells. 20% of IP loaded. Antibodies : Affinity purified rabbit anti-PDE8B antibody (ab112024) at 1 μg/ml. Lane 1- PDE8B was immunoprecipitated by rabbit anti-PDE8B antibody (ab112024) at 6 mcg/mg lysate. Lane 2- PDE8B was immunoprecipitated by an rabbit anti-PDE8B antibody that recognizes an upstream epitope. Lane 3- Rabbit IgG control. Detection : Chemiluminescence with exposure times of 3 minutes.

All lanes:

Immunoprecipitation - Anti-PDE8B antibody (ab112024)

Predicted band size: 98 kDa

false

Western blot - Anti-PDE8B antibody (AB112024)
  • WB

Unknown

Western blot - Anti-PDE8B antibody (AB112024)

All lanes:

Western blot - Anti-PDE8B antibody (ab112024) at 0.4 µg/mL

Lane 1:

Whole cell extracts from HeLa cells at 50 µg

Lane 2:

Whole cell extracts from HeLa cells at 15 µg

Lane 3:

Whole cell extracts from HeLa cells at 5 µg

Predicted band size: 98 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

WB, IP

applications

Immunogen

Synthetic Peptide within Human PDE8B aa 800 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

O95263

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  • 4
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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "2-5 µg/mg of lysate", "IP-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/2000 - 1/10000", "WB-species-notes": "<p></p>" }, "Chimpanzee": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Cow": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Dog": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Gorilla": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Guinea pig": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Horse": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Orangutan": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Pig": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Rhesus monkey": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
pH: 7 - 8 Preservative: 0.09% Sodium azide Constituents: 99% Tris citrate/phosphate
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

PDE8B also known as phosphodiesterase 8B is an enzyme that hydrolyzes cAMP an important second messenger. It plays a significant role in regulating cellular responses to hormonal stimulation by degrading cAMP which in turn modulates cellular signaling. The protein has a molecular mass of approximately 100 kDa. PDE8B expression is high in the thyroid gland and is also found in the brain and testis reflecting its possible various physiological roles.
Biological function summary

PDE8B impacts several critical cellular processes due to its enzymatic role in cAMP degradation. It is a part of the phosphodiesterase enzyme family which includes 11 diverse families of phosphodiesterases. PDE8B's function supports cellular proliferation differentiation and apoptosis control by influencing cAMP levels within the cell. Its activity is not typically associated with any macromolecular complexes but it contributes to the broader regulatory network controlling cAMP-mediated signaling pathways.

Pathways

PDE8B participates in the cAMP signaling pathway which is vital for mediating the effects of neurotransmitters and hormones. The regulation of this pathway by PDE8B affects processes like energy homeostasis inflammation and neurotransmitter release. PDE8B interacts closely with other members of the phosphodiesterase family particularly PDE4 and PDE10 in fine-tuning the cAMP degradation and ensuring precise control of signal transduction.

Mutations in PDE8B are connected to thyroid-related abnormalities such as congenital hypothyroidism. This condition often links to disruptions in cAMP levels impacting thyroid hormone production. Research also suggests a possible association of PDE8B with adrenal diseases where it influences the stress response by affecting cortisol synthesis. In these contexts PDE8B might interact with proteins such as thyroid-stimulating hormone receptor (TSHR) in thyroid conditions and adrenocorticotropic hormone (ACTH) in adrenal functions showcasing its multifaceted role in disease mechanisms.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Hydrolyzes the second messenger cAMP, which is a key regulator of many important physiological processes. May be involved in specific signaling in the thyroid gland.
See full target information PDE8B
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Gastroenterology 153:1555-1567.e15 PubMed28859856

2017

Gastrin Induces Nuclear Export and Proteasome Degradation of Menin in Enteric Glial Cells.

Applications

Unspecified application

Species

Unspecified reactive species

Sinju Sundaresan,Cameron A Meininger,Anthony J Kang,Amanda L Photenhauer,Michael M Hayes,Nirakar Sahoo,Jolanta Grembecka,Tomasz Cierpicki,Lin Ding,Thomas J Giordano,Tobias Else,David J Madrigal,Malcolm J Low,Fiona Campbell,Ann-Marie Baker,Haoxing Xu,Nicholas A Wright,Juanita L Merchant
View all publications
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Product promise

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