JavaScript is disabled in your browser. Please enable JavaScript to view this website.
AB168432

Anti-PDE9A antibody

Be the first to review this product! Submit a review

|

(2 Publications)

Mouse Polyclonal PDE9A antibody. Suitable for WB, ICC/IF and reacts with Human samples. Cited in 2 publications. Immunogen corresponding to Recombinant Full Length Protein corresponding to Human PDE9A.

View Alternative Names

PDE9A

2 Images
Immunocytochemistry/ Immunofluorescence - Anti-PDE9A antibody (AB168432)
  • ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-PDE9A antibody (AB168432)

Immunofluorescence analysis of HeLa cells labeling PDE9A with ab168432 at 10μg/ml.

Western blot - Anti-PDE9A antibody (AB168432)
  • WB

Unknown

Western blot - Anti-PDE9A antibody (AB168432)

All lanes:

Western blot - Anti-PDE9A antibody (ab168432) at 1 µg/mL

Lane 1:

PDE9A transfected 293T lysate at 15 µL

Lane 2:

Non-transfected 293T lysate at 15 µL

Predicted band size: 68 kDa

false

Key facts

Host species

Mouse

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

ICC/IF, WB

applications

Immunogen

Recombinant Full Length Protein corresponding to Human PDE9A.

O76083

style
left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1 µg/mL", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "10 µg/mL", "ICCIF-species-notes": "<p></p>" }, "Mouse": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }
style
left-column
style
left-column

Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.4 Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
style
left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

PDE9A is a phosphodiesterase enzyme that breaks down cyclic guanosine monophosphate (cGMP) into non-cyclic GMP therefore regulating intracellular levels of cGMP. This enzyme has a molecular mass of approximately 65 kilodaltons. Researchers often refer to PDE9A also as phosphodiesterase 9A. It shows higher expression in the brain and heart with presence also noted in the kidneys and lungs.
Biological function summary

PDE9A regulates the signaling processes by controlling cGMP turnover. It functions as a part of a larger network of proteins involved in intracellular signal transduction. PDE9A does not typically form distinct complexes but interacts with signaling components to maintain balanced cGMP concentrations which is essential for signaling related to vasodilation and platelet aggregation.

Pathways

PDE9A influences both cardiovascular and central nervous system pathways. In the cardiovascular system it interacts with nitric oxide signaling which has connections to blood pressure regulation through vasodilation. Within the CNS PDE9A is involved in synaptic plasticity relating to proteins like phosphodiesterase PDE5 and PDE2 which also handle cyclic nucleotide breakdown affecting neurotransmitter signaling and learning processes.

PDE9A has connections to conditions like heart failure and Alzheimer’s disease. In heart failure PDE9A’s regulation of cGMP affects nitric oxide signaling and cardiac function. In Alzheimer’s disease its influence on synaptic plasticity through interactions with proteins like amyloid precursor protein (APP) suggests a role in cognitive decline making it a potential therapeutic target for modulating synaptic signaling and cognitive function.
style
left-column
style
left-column

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
style
left-column

Target data

Specifically hydrolyzes the second messenger cGMP, which is a key regulator of many important physiological processes. Highly specific : compared to other members of the cyclic nucleotide phosphodiesterase family, has the highest affinity and selectivity for cGMP (PubMed : 18757755, PubMed : 21483814, PubMed : 9624146). Specifically regulates natriuretic-peptide-dependent cGMP signaling in heart, acting as a regulator of cardiac hypertrophy in myocytes and muscle. Does not regulate nitric oxide-dependent cGMP in heart (PubMed : 25799991). Additional experiments are required to confirm whether its ability to hydrolyze natriuretic-peptide-dependent cGMP is specific to heart or is a general feature of the protein (Probable). In brain, involved in cognitive function, such as learning and long-term memory (By similarity).
See full target information PDE9A
style
left-column

Publications (2)

Recent publications for all applications. Explore the full list and refine your search

The EMBO journal 44:1249-1273 PubMed39806097

2025

E3 ubiquitin ligase CHIP facilitates cAMP and cGMP signalling cross-talk by polyubiquitinating PDE9A.

Applications

Unspecified application

Species

Unspecified reactive species

Xiaoyan Hao,Zhengwei Hu,Mengjie Li,Shuo Zhang,Mibo Tang,Chenwei Hao,Shasha Qi,Yuanyuan Liang,Michael F Almeida,Kaitlan Smith,Chunyan Zuo,Yanmei Feng,Mengnan Guo,Dongrui Ma,Shuangjie Li,Zhiyun Wang,Yuemeng Sun,Zhifen Deng,Chengyuan Mao,Zongping Xia,Yong Jiang,Yanxia Gao,Yuming Xu,Jonathan C Schisler,Changhe Shi

The Journal of biological chemistry 300:107783 PubMed39303913

2024

Downregulation of the mA reader YTHDC2 upregulates exosome content in lung adenocarcinoma via inhibiting IFIT and OAS family members.

Applications

Unspecified application

Species

Unspecified reactive species

Zhixin Yin,Lifang Ma,Xiaoting Tian,Qi Sun,Congcong Zhang,Yikun Wang,Yayou Miao,Xiangfei Xue,Yongjie Wang,Jiayi Wang,Xiao Zhang,Xumin Hou
View all publications
style
left-column
style
left-column
style
right-column

Alternative Products

Primary Antibodies

AB97556

Anti-PDE9A antibody

Western blot - Anti-PDE9A antibody (AB97556)

  • 0
  • 0

Complementary Products

Primary Antibodies

AB170914

Anti-PDE7B antibody [EPR11491]

RabMAb|Recombinant

Flow Cytometry (Intracellular) - Anti-PDE7B antibody [EPR11491] (AB170914)

  • 5
  • 1
Primary Antibodies

AB182565

Anti-PDE1B antibody [EPR12293(2)]

RabMAb|Recombinant

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PDE1B antibody [EPR12293(2)] (AB182565)

  • 4
  • 1
Primary Antibodies

AB96825

Anti-PDE6D antibody

Immunocytochemistry/ Immunofluorescence - Anti-PDE6D antibody (AB96825)

  • 0
  • 0
Primary Antibodies

AB248930

Anti-PDE2A antibody [EPR9688] - BSA and Azide free

RabMAb|Recombinant

Western blot - Anti-PDE2A antibody [EPR9688] - BSA and Azide free (AB248930)

  • 0
  • 0

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com