Rabbit Polyclonal PDGF-AA antibody. Suitable for WB, IHC-P and reacts with Mouse, Human samples. Cited in 4 publications. Immunogen corresponding to Synthetic Peptide within Human PDGFA aa 100-200 conjugated to Keyhole Limpet Haemocyanin.
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Publications
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- Physiological reports 11:2023The effects of treadmill exercise on brain angiogenesis in ovariectomized rats.Applications:Unspecified applicationReactive species:Unspecified reactive speciesEun-Jung Yoon,Jiwon Jeong,Eunji Yoon,Dongsun ParkPubMed 37962017
- Journal of orthopaedic research : official publica :2021The Effect of Estrogen-Like Compound on Rotator Cuff Tendon Healing in a Murine Model.Applications:Unspecified applicationReactive species:Unspecified reactive speciesRobert Z Tashjian et. al.PubMed 33533088
- World journal of stem cells 12:633-6582020Bone marrow mesenchymal stem cells induce M2 microglia polarization through PDGF-AA/MANF signaling.Applications:Unspecified applicationReactive species:Unspecified reactive speciesFan Yang,Wen-Bin Li,Ye-Wei Qu,Jin-Xing Gao,Yu-Shi Tang,Dong-Jie Wang,Yu-Jun PanPubMed 32843919
- Journal of cellular physiology 234:17946-179582019Reduced PDGF-AA in subchondral bone leads to articular cartilage degeneration after strenuous running.Applications:Unspecified applicationReactive species:Unspecified reactive speciesZilong Yao et. al.PubMed 30834523
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.4
Preservative: 0.02% Proclin 300
Constituents: 50% Glycerol (glycerin, glycerine), 48.98% TBS, 1X, 1% BSA- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- Synthetic Peptide within Human PDGFA aa 100-200 conjugated to Keyhole Limpet Haemocyanin. The exact immunogen used to generate this antibody is proprietary information. Database link P04085
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Reactivity data
Select an application| WB | IHC-P | |
|---|---|---|
| Human | Expected | Tested |
| Mouse | Tested | Expected |
| Rat | Predicted | Predicted |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info 1/100 - 1/1000 | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info Use at an assay dependent concentration. | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Rat | Dilution info - | Notes - |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/100 - 1/500 | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info Use at an assay dependent concentration. | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Rat | Dilution info - | Notes - |
Associated Products
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Target data
Function
Growth factor that plays an essential role in the regulation of embryonic development, cell proliferation, cell migration, survival and chemotaxis. Potent mitogen for cells of mesenchymal origin. Required for normal lung alveolar septum formation during embryogenesis, normal development of the gastrointestinal tract, normal development of Leydig cells and spermatogenesis. Required for normal oligodendrocyte development and normal myelination in the spinal cord and cerebellum. Plays an important role in wound healing. Signaling is modulated by the formation of heterodimers with PDGFB (By similarity).
Alternative names
PDGF1, PDGFA, Platelet-derived growth factor subunit A, PDGF subunit A, PDGF-1, Platelet-derived growth factor A chain, Platelet-derived growth factor alpha polypeptide
Recommended products
Rabbit Polyclonal PDGF-AA antibody. Suitable for WB, IHC-P and reacts with Mouse, Human samples. Cited in 4 publications. Immunogen corresponding to Synthetic Peptide within Human PDGFA aa 100-200 conjugated to Keyhole Limpet Haemocyanin.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.4
Preservative: 0.02% Proclin 300
Constituents: 50% Glycerol (glycerin, glycerine), 48.98% TBS, 1X, 1% BSA- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- Synthetic Peptide within Human PDGFA aa 100-200 conjugated to Keyhole Limpet Haemocyanin. The exact immunogen used to generate this antibody is proprietary information. Database link P04085
- Purification technique
- Affinity purification Protein A
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
Platelet-derived growth factor AA or PDGF-AA is a member of the PDGF protein family which is involved in various cellular processes. The PDGF-AA protein has a molecular mass of approximately 24 kDa. It is expressed in a wide range of tissues including fibroblasts smooth muscle cells and neurons. The protein is considered a homodimer composed of two A chains and it plays an important role in cell signaling by binding to PDGF receptors on the surface of target cells.
- Biological function summary
PDGF-AA influences cell proliferation migration and differentiation. It acts as a mitogen especially for mesenchymal cells such as fibroblasts and smooth muscle cells. PDGF-AA is often part of a signaling complex where it activates PDGF receptor-alpha (PDGFR-α) leading to autophosphorylation and subsequent activation of downstream effectors. This strengthens tissue development and wound healing processes.
- Pathways
PDGF-AA integrates into critical signaling pathways like the PI3K/AKT pathway and the MAPK/ERK pathway. These pathways regulate cell growth and survival. PDGF-AA binds specifically to PDGFR-α which shares importance with other PDGF isoforms such as PDGF-BB in orchestrating cellular responses through similar pathways thereby ensuring precise physiological outcomes.
- Associated diseases and disorders
PDGF-AA is implicated in conditions like cancer and fibrosis. Aberrant signaling involving PDGF-AA can lead to unchecked cell proliferation contributing to tumorigenesis. Similarly PDGF-AA is connected to fibrotic diseases where excessive tissue scarring occurs due to overactive fibroblast stimulation. PDGF-BB another member of the PDGF protein family often gets studied alongside PDGF-AA for their combined roles in these pathological states.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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In the unlikely event of one of our products not working as expected, you are covered by our product promise.
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3 product images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PDGF AA antibody (ab216619)
Immunohistochemical analysis of formalin-fixed, paraffin-embedded human colon tubular adenocarcinoma labeling PDGF AA with ab216619 at 1/300 dilution, followed by conjugation to the secondary antibody and DAB staining.
Western blot - Anti-PDGF AA antibody (ab216619)
All lanes: Western blot - Anti-PDGF AA antibody (ab216619) at 1/300 dilution
All lanes: Mouse lung lysate
SecondaryAll lanes: Goat anti-rabbit IgG (H+L), HRP conjugated at 1/5000 dilution
Predicted band size: 24 kDa
Western blot - Anti-PDGF AA antibody (ab216619)
All lanes: Western blot - Anti-PDGF AA antibody (ab216619) at 1/300 dilution
All lanes: Mouse placenta lysate
SecondaryAll lanes: Goat anti-rabbit IgG (H+L), HRP conjugated at 1/5000 dilution
Predicted band size: 24 kDa
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