AB216619

Anti-PDGF AA antibody

Name: Anti-PDGF AA antibody (ab216619) | Abcam
Brand: Abcam Limited
SKU: AB216619
Price: 525 USD
Availability: InStock
Rating: 4 (1 reviews)

(1 Review)

(4 Publications)

Rabbit Polyclonal PDGF-AA antibody. Suitable for WB, IHC-P and reacts with Mouse, Human samples. Cited in 4 publications. Immunogen corresponding to Synthetic Peptide within Human PDGFA aa 100-200 conjugated to Keyhole Limpet Haemocyanin.

View Alternative Names

PDGF1, PDGFA, Platelet-derived growth factor subunit A, PDGF subunit A, PDGF-1, Platelet-derived growth factor A chain, Platelet-derived growth factor alpha polypeptide

3 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PDGF AA antibody (AB216619)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PDGF AA antibody (AB216619)

Immunohistochemical analysis of formalin-fixed, paraffin-embedded human colon tubular adenocarcinoma labeling PDGF AA with ab216619 at 1/300 dilution, followed by conjugation to the secondary antibody and DAB staining.

Supplier Data

Western blot - Anti-PDGF AA antibody (AB216619)

All lanes:

Western blot - Anti-PDGF AA antibody (ab216619) at 1/300 dilution

All lanes:

Mouse placenta lysate

Secondary

All lanes:

Goat anti-rabbit IgG (H+L), HRP conjugated at 1/5000 dilution

Predicted band size: 24 kDa

false

Supplier Data

Western blot - Anti-PDGF AA antibody (AB216619)

All lanes:

Western blot - Anti-PDGF AA antibody (ab216619) at 1/300 dilution

All lanes:

Mouse lung lysate

Secondary

All lanes:

Goat anti-rabbit IgG (H+L), HRP conjugated at 1/5000 dilution

Predicted band size: 24 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Mouse, Human

Applications

WB, IHC-P

applications

Immunogen

Synthetic Peptide within Human PDGFA aa 100-200 conjugated to Keyhole Limpet Haemocyanin. The exact immunogen used to generate this antibody is proprietary information.

P04085

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/500", "IHCP-species-notes": "<p></p>" }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/100 - 1/1000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Rat": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.4 Preservative: 0.02% Proclin 300 Constituents: 50% Glycerol (glycerin, glycerine), 48.98% TBS, 1X, 1% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Platelet-derived growth factor AA or PDGF-AA is a member of the PDGF protein family which is involved in various cellular processes. The PDGF-AA protein has a molecular mass of approximately 24 kDa. It is expressed in a wide range of tissues including fibroblasts smooth muscle cells and neurons. The protein is considered a homodimer composed of two A chains and it plays an important role in cell signaling by binding to PDGF receptors on the surface of target cells.

Biological function summary

PDGF-AA influences cell proliferation migration and differentiation. It acts as a mitogen especially for mesenchymal cells such as fibroblasts and smooth muscle cells. PDGF-AA is often part of a signaling complex where it activates PDGF receptor-alpha (PDGFR-α) leading to autophosphorylation and subsequent activation of downstream effectors. This strengthens tissue development and wound healing processes.

Pathways

PDGF-AA integrates into critical signaling pathways like the PI3K/AKT pathway and the MAPK/ERK pathway. These pathways regulate cell growth and survival. PDGF-AA binds specifically to PDGFR-α which shares importance with other PDGF isoforms such as PDGF-BB in orchestrating cellular responses through similar pathways thereby ensuring precise physiological outcomes.

PDGF-AA is implicated in conditions like cancer and fibrosis. Aberrant signaling involving PDGF-AA can lead to unchecked cell proliferation contributing to tumorigenesis. Similarly PDGF-AA is connected to fibrotic diseases where excessive tissue scarring occurs due to overactive fibroblast stimulation. PDGF-BB another member of the PDGF protein family often gets studied alongside PDGF-AA for their combined roles in these pathological states.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Growth factor that plays an essential role in the regulation of embryonic development, cell proliferation, cell migration, survival and chemotaxis. Potent mitogen for cells of mesenchymal origin. Required for normal lung alveolar septum formation during embryogenesis, normal development of the gastrointestinal tract, normal development of Leydig cells and spermatogenesis. Required for normal oligodendrocyte development and normal myelination in the spinal cord and cerebellum. Plays an important role in wound healing. Signaling is modulated by the formation of heterodimers with PDGFB (By similarity).

See full target information PDGFA

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Publications (4)

Recent publications for all applications. Explore the full list and refine your search

Physiological reports 11: PubMed37962017

2023

The effects of treadmill exercise on brain angiogenesis in ovariectomized rats.

Applications

Unspecified application

Species

Unspecified reactive species

Eun-Jung Yoon,Jiwon Jeong,Eunji Yoon,Dongsun Park

Journal of orthopaedic research : official publica : PubMed33533088

2021

The Effect of Estrogen-Like Compound on Rotator Cuff Tendon Healing in a Murine Model.

Applications

Unspecified application

Species

Unspecified reactive species

Robert Z Tashjian,Nikolas H Kazmers,Richard T Epperson,Matthew Honeggar,Ying Ma,Peter N Chalmers,Dustin L Williams,Michael J Jurynec

World journal of stem cells 12:633-658 PubMed32843919

2020

Bone marrow mesenchymal stem cells induce M2 microglia polarization through PDGF-AA/MANF signaling.

Applications

Unspecified application

Species

Unspecified reactive species

Fan Yang,Wen-Bin Li,Ye-Wei Qu,Jin-Xing Gao,Yu-Shi Tang,Dong-Jie Wang,Yu-Jun Pan

Journal of cellular physiology 234:17946-17958 PubMed30834523

2019

Reduced PDGF-AA in subchondral bone leads to articular cartilage degeneration after strenuous running.

Applications

Unspecified application

Species

Unspecified reactive species

Zilong Yao,Peisheng Chen,Shengnan Wang,Ganming Deng,Yanjun Hu,Qingrong Lin,Xianrong Zhang,Bin Yu

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-PDGF AA antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PDGF AA antibody (AB216619)

Western blot - Anti-PDGF AA antibody (AB216619)

Western blot - Anti-PDGF AA antibody (AB216619)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

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Secondary Antibodies

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Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (4)

Product promise