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AB251407

Anti-PDGF AA antibody [EPR19924] - BSA and Azide free

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(1 Publication)

Rabbit Recombinant Monoclonal PDGF-AA antibody. Carrier free. Suitable for IP and reacts with Human samples. Cited in 1 publication.

View Alternative Names

PDGF1, PDGFA, Platelet-derived growth factor subunit A, PDGF subunit A, PDGF-1, Platelet-derived growth factor A chain, Platelet-derived growth factor alpha polypeptide

1 Images
Immunoprecipitation - Anti-PDGF AA antibody [EPR19924] - BSA and Azide free (AB251407)
  • IP

Supplier Data

Immunoprecipitation - Anti-PDGF AA antibody [EPR19924] - BSA and Azide free (AB251407)

This data was developed using ab203911, the same antibody clone in a different buffer formulation.

PDGF AA was immunoprecipitated from 0.35 mg of Human kidney lysate with ab203911 at 1/30 dilution. Western blot was performed from the immunoprecipitate using ab203911 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/10000 dilution.

Lane 1 : Human kidney lysate, 10 μg (Input).
Lane 2 : ab203911 IP in Human kidney lysate.
Lane 3 : Rabbit monoclonal IgG (ab172730) instead of ab203911 in Human kidney lysate.

Blocking and dilution buffer and concentration : 5% NFDM/TBST.

Exposure time : 3 seconds.

All lanes:

Immunoprecipitation - Anti-PDGF AA antibody [EPR19924] (<a href='/en-us/products/primary-antibodies/pdgf-aa-antibody-epr19924-ab203911'>ab203911</a>)

Predicted band size: 24 kDa

false

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR19924

Isotype

IgG

Carrier free

Yes

Reacts with

Human

Applications

IP

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

Specificity

This antibody does not react with human species in the Western Blot application.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "", "IP-species-notes": "<p></p>", "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>" } } }
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Product details

ab251407 is the carrier-free version of ab203911.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 - 7.4 Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
Do Not Freeze
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Platelet-derived growth factor AA or PDGF-AA is a member of the PDGF protein family which is involved in various cellular processes. The PDGF-AA protein has a molecular mass of approximately 24 kDa. It is expressed in a wide range of tissues including fibroblasts smooth muscle cells and neurons. The protein is considered a homodimer composed of two A chains and it plays an important role in cell signaling by binding to PDGF receptors on the surface of target cells.
Biological function summary

PDGF-AA influences cell proliferation migration and differentiation. It acts as a mitogen especially for mesenchymal cells such as fibroblasts and smooth muscle cells. PDGF-AA is often part of a signaling complex where it activates PDGF receptor-alpha (PDGFR-α) leading to autophosphorylation and subsequent activation of downstream effectors. This strengthens tissue development and wound healing processes.

Pathways

PDGF-AA integrates into critical signaling pathways like the PI3K/AKT pathway and the MAPK/ERK pathway. These pathways regulate cell growth and survival. PDGF-AA binds specifically to PDGFR-α which shares importance with other PDGF isoforms such as PDGF-BB in orchestrating cellular responses through similar pathways thereby ensuring precise physiological outcomes.

PDGF-AA is implicated in conditions like cancer and fibrosis. Aberrant signaling involving PDGF-AA can lead to unchecked cell proliferation contributing to tumorigenesis. Similarly PDGF-AA is connected to fibrotic diseases where excessive tissue scarring occurs due to overactive fibroblast stimulation. PDGF-BB another member of the PDGF protein family often gets studied alongside PDGF-AA for their combined roles in these pathological states.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Growth factor that plays an essential role in the regulation of embryonic development, cell proliferation, cell migration, survival and chemotaxis. Potent mitogen for cells of mesenchymal origin. Required for normal lung alveolar septum formation during embryogenesis, normal development of the gastrointestinal tract, normal development of Leydig cells and spermatogenesis. Required for normal oligodendrocyte development and normal myelination in the spinal cord and cerebellum. Plays an important role in wound healing. Signaling is modulated by the formation of heterodimers with PDGFB (By similarity).
See full target information PDGFA
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Nature communications 12:3596 PubMed34155205

2021

ERRγ enhances cardiac maturation with T-tubule formation in human iPSC-derived cardiomyocytes.

Applications

Unspecified application

Species

Unspecified reactive species

Kenji Miki,Kohei Deguchi,Misato Nakanishi-Koakutsu,Antonio Lucena-Cacace,Shigeru Kondo,Yuya Fujiwara,Takeshi Hatani,Masako Sasaki,Yuki Naka,Chikako Okubo,Megumi Narita,Ikue Takei,Stephanie C Napier,Tsukasa Sugo,Sachiko Imaichi,Taku Monjo,Tatsuya Ando,Norihisa Tamura,Kenichi Imahashi,Tomoyuki Nishimoto,Yoshinori Yoshida
View all publications
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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