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Rabbit Polyclonal PDGF B antibody. Suitable for IHC-P, WB, IHC-Fr and reacts with Human, Recombinant full length protein - Human, Mouse samples. Cited in 56 publications.
Alternative names=Platelet-derived growth factor subunit B, PDGF subunit B, PDGF-2, Platelet-derived growth factor B chain, Platelet-derived growth factor beta polypeptide, Proto-oncogene c-Sis, PDGFB, SIS, PDGF2
- Isotype
IgG
- Host species
Rabbit
- Storage buffer
pH: 7.4
Preservative: 0.02% Sodium azide
Constituents: 98.98% PBS, 1% BSA- Form
Liquid
- Clonality
Polyclonal
- Immunogen
The exact immunogen used to generate this antibody is proprietary information.
Consider this alternative
Reactivity data
Select an application| IHC-P | WB | IHC-Fr | |
|---|---|---|---|
| Human | Tested | Expected | Expected |
| Mouse | Predicted | Predicted | Expected |
| Cow | Predicted | Predicted | Predicted |
| Recombinant full length protein - Human | Not recommended | Tested | Not recommended |
Images
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 5 µg/mL | Notes Perform heat-mediated antigen retrieval before commencing with IHC staining protocol. |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Cow, Mouse | Dilution info - | Notes - |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Recombinant full length protein - Human | Dilution info - | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Recombinant full length protein - Human | Dilution info 1 µg/mL | Notes The predicted molecular weight of PDGF B is ~ 27 kDa according to the Swissprot entry P01127, however the reduced form of PDGF B runs between 14-18 kDa on a gel. |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1 µg/mL | Notes The predicted molecular weight of PDGF B is ~ 27 kDa according to the Swissprot entry P01127, however the reduced form of PDGF B runs between 14-18 kDa on a gel. |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Cow, Mouse | Dilution info - | Notes - |
IHC-Fr
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Human | Dilution info Use at an assay dependent concentration. | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Cow | Dilution info - | Notes - |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Recombinant full length protein - Human | Dilution info - | Notes - |
Target data
Function
Growth factor that plays an essential role in the regulation of embryonic development, cell proliferation, cell migration, survival and chemotaxis. Potent mitogen for cells of mesenchymal origin (PubMed:26599395). Required for normal proliferation and recruitment of pericytes and vascular smooth muscle cells in the central nervous system, skin, lung, heart and placenta. Required for normal blood vessel development, and for normal development of kidney glomeruli. Plays an important role in wound healing. Signaling is modulated by the formation of heterodimers with PDGFA (By similarity).
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Rabbit Polyclonal PDGF B antibody. Suitable for IHC-P, WB, IHC-Fr and reacts with Human, Recombinant full length protein - Human, Mouse samples. Cited in 56 publications.
Alternative names=Platelet-derived growth factor subunit B, PDGF subunit B, PDGF-2, Platelet-derived growth factor B chain, Platelet-derived growth factor beta polypeptide, Proto-oncogene c-Sis, PDGFB, SIS, PDGF2
Key facts
- Isotype
IgG
- Host species
Rabbit
- Storage buffer
pH: 7.4
Preservative: 0.02% Sodium azide
Constituents: 98.98% PBS, 1% BSA- Form
Liquid
- Clonality
Polyclonal
- Immunogen
The exact immunogen used to generate this antibody is proprietary information.
- Purification technique
Affinity purification Immunogen
- Specificity
This antibody has been tested against PDGF B protein (ab9706) and has yet to be tested against endogenous protein. From July 2024, QC testing of replenishment batches of this polyclonal changed. All tested and expected application and reactive species combinations are still covered by our Abcam product promise. However, we no longer test all applications. For more information on a specific batch, please contact our Scientific Support who will be happy to help.
- Concentration
Storage
- Shipped at conditions
Blue Ice
- Appropriate short-term storage duration
1-2 weeks
- Appropriate short-term storage conditions
+4°C
- Appropriate long-term storage conditions
-20°C
- Aliquoting information
Upon delivery aliquot
- Storage information
Avoid freeze / thaw cycle
Notes
Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.
If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.
Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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In the unlikely event of one of our products not working as expected, you are covered by our product promise.
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