Anti-PDGF B antibody (ab23914) is a rabbit polyclonal antibody detecting PDGF B in Western Blot, IHC-P, IHC-Fr. Suitable for Human,.
- Over 50 publications
- Trusted since 2006
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- The Journal of clinical investigation 133:2023Bone-derived PDGF-BB drives brain vascular calcification in male mice.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJiekang Wang,Ching-Lien Fang,Kathleen Noller,Zhiliang Wei,Guanqiao Liu,Ke Shen,Kangping Song,Xu Cao,Mei WanPubMed 37815871
- Physiological reports 11:2023The effects of treadmill exercise on brain angiogenesis in ovariectomized rats.Applications:Unspecified applicationReactive species:Unspecified reactive speciesEun-Jung Yoon,Jiwon Jeong,Eunji Yoon,Dongsun ParkPubMed 37962017
- Arthritis research & therapy 25:1942023Preosteoclast plays a pathogenic role in syndesmophyte formation of ankylosing spondylitis through the secreted PDGFB - GRB2/ERK/RUNX2 pathway.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYulong Tang,Kai Yang,Qingmei Liu,Yanyun Ma,Hao Zhu,Kunhai Tang,Chengchun Geng,Jiangnan Xie,Dachun Zhuo,Wenyu Wu,Li Jin,Wenze Xiao,Jiucun Wang,Qi Zhu,Jing LiuPubMed 37798786
- Journal of translational medicine 21:6672023Tumor microenvironment in a minipig model of spinal cord glioma.Applications:Unspecified applicationReactive species:Unspecified reactive speciesMuhibullah S Tora,Stewart G Neill,Yuliya Lakhina,Hemza Assed,Michelle Zhang,Purva P Nagarajan,Thais Federici,Juanmarco Gutierrez,Kimberly B Hoang,Yuhong Du,Kecheng Lei,Nicholas M BoulisPubMed 37752585
- The Journal of clinical investigation 133:2023Pancreatic RECK inactivation promotes cancer formation, epithelial-mesenchymal transition, and metastasis.Applications:Unspecified applicationReactive species:Unspecified reactive speciesTomonori Masuda,Akihisa Fukuda,Go Yamakawa,Mayuki Omatsu,Mio Namikawa,Makoto Sono,Yuichi Fukunaga,Munemasa Nagao,Osamu Araki,Takaaki Yoshikawa,Satoshi Ogawa,Kenji Masuo,Norihiro Goto,Yukiko Hiramatsu,Yu Muta,Motoyuki Tsuda,Takahisa Maruno,Yuki Nakanishi,Toshihiko Masui,Etsuro Hatano,Tomoko Matsuzaki,Makoto Noda,Hiroshi SenoPubMed 37712427
- Nature communications 14:19572023ASPSCR1::TFE3 orchestrates the angiogenic program of alveolar soft part sarcoma.Applications:Unspecified applicationReactive species:Unspecified reactive speciesMiwa Tanaka,Surachada Chuaychob,Mizuki Homme,Yukari Yamazaki,Ruyin Lyu,Kyoko Yamashita,Keisuke Ae,Seiichi Matsumoto,Kohei Kumegawa,Reo Maruyama,Wei Qu,Yohei Miyagi,Ryuji Yokokawa,Takuro NakamuraPubMed 37029109
- Experimental dermatology 32:878-8882023Topical application of imatinib mesylate ameliorated psoriasis-like skin lesions in imiquimod-induced murine model via angiogenesis inhibition.Applications:Unspecified applicationReactive species:Unspecified reactive speciesKazuma Hayakawa,Tatsuo Maeda,Chizu Egusa,Yukari Okubo,Kazutoshi HaradaPubMed 36928929
- iScience 25:1049212022Multi-omics characterization reveals the pathogenesis of liver focal nodular hyperplasia.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYuming Liu,Jinmai Zhang,Zhuo Wang,Jiaqiang Ma,Ke Wang,Dongning Rao,Mao Zhang,Youpei Lin,Yingcheng Wu,Zijian Yang,Liangqing Dong,Zhenbin Ding,Xiaoming Zhang,Jia Fan,Yongyong Shi,Qiang GaoPubMed 36060063
- Frontiers in immunology 13:9076362022Spindle pole body component 25 and platelet-derived growth factor mediate crosstalk between tumor-associated macrophages and prostate cancer cells.Applications:Unspecified applicationReactive species:Unspecified reactive speciesFeilun Cui,Zhipeng Xu,Jianpeng Hu,Yumei LvPubMed 35967419
- Cell death & disease 13:4172022ICAM-1 promotes cancer progression by regulating SRC activity as an adapter protein in colorectal cancer.Applications:Unspecified applicationReactive species:Unspecified reactive speciesEun-Ji Lim,Jae-Hyeok Kang,Yeon-Ju Kim,Seungmo Kim,Su-Jae LeePubMed 35487888
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.4
Preservative: 0.02% Sodium azide
Constituents: 98.98% PBS, 1% BSA- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
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Reactivity data
Select an application| IHC-P | WB | IHC-Fr | |
|---|---|---|---|
| Human | Tested | Expected | Expected |
| Mouse | Predicted | Predicted | Expected |
| Cow | Predicted | Predicted | Predicted |
| Recombinant full length protein - Human | Not recommended | Tested | Not recommended |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 5 µg/mL | Notes Perform heat-mediated antigen retrieval before commencing with IHC staining protocol. |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Cow, Mouse | Dilution info - | Notes - |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Recombinant full length protein - Human | Dilution info - | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Recombinant full length protein - Human | Dilution info 1 µg/mL | Notes The predicted molecular weight of PDGF B is ~ 27 kDa according to the Swissprot entry P01127, however the reduced form of PDGF B runs between 14-18 kDa on a gel. |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1 µg/mL | Notes The predicted molecular weight of PDGF B is ~ 27 kDa according to the Swissprot entry P01127, however the reduced form of PDGF B runs between 14-18 kDa on a gel. |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Cow, Mouse | Dilution info - | Notes - |
IHC-Fr
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Human | Dilution info Use at an assay dependent concentration. | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Cow | Dilution info - | Notes - |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Recombinant full length protein - Human | Dilution info - | Notes - |
Associated Products
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Target data
Function
Growth factor that plays an essential role in the regulation of embryonic development, cell proliferation, cell migration, survival and chemotaxis. Potent mitogen for cells of mesenchymal origin (PubMed:26599395). Required for normal proliferation and recruitment of pericytes and vascular smooth muscle cells in the central nervous system, skin, lung, heart and placenta. Required for normal blood vessel development, and for normal development of kidney glomeruli. Plays an important role in wound healing. Signaling is modulated by the formation of heterodimers with PDGFA (By similarity).
Alternative names
PDGF2, SIS, PDGFB, Platelet-derived growth factor subunit B, PDGF subunit B, PDGF-2, Platelet-derived growth factor B chain, Platelet-derived growth factor beta polypeptide, Proto-oncogene c-Sis
Recommended products
Anti-PDGF B antibody (ab23914) is a rabbit polyclonal antibody detecting PDGF B in Western Blot, IHC-P, IHC-Fr. Suitable for Human,.
- Over 50 publications
- Trusted since 2006
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.4
Preservative: 0.02% Sodium azide
Constituents: 98.98% PBS, 1% BSA- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Purification technique
- Affinity purification Immunogen
- Specificity
This antibody has been tested against PDGF B protein (ab9706) and has yet to be tested against endogenous protein. From July 2024, QC testing of replenishment batches of this polyclonal changed. All tested and expected application and reactive species combinations are still covered by our Abcam product promise. However, we no longer test all applications. For more information on a specific batch, please contact our Scientific Support who will be happy to help.
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
Anti-PDGF B antibody (ab23914) is a rabbit polyclonal antibody and is validated for use in Western Blot (WB), Immunohistochemistry (IHC-P), Immunohistochemistry (IHC-Fr) in Human, samples.
What is the molecular weight of PDGF B?
Anti-PDGF B (ab23914) specifically detects a band for PDGF B (UniProt: P01127) at a molecular weight of 14-18kDa.
Trusted by the scientific community
Anti-PDGF B (ab23914) was first used in a scientific publication in 2006 and has been cited over 50 times in peer-reviewed journals.
Reviewed by scientists
Anti-PDGF B (ab23914) has over 10 independent reviews from customers.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
PDGF-B also known as platelet-derived growth factor subunit B or PDGF-BB is a critical protein involved in various cellular processes. Composed of 241 amino acids PDGF-B exhibits a molecular mass of approximately 27 kDa. Scientists recognize this protein for its expression primarily in platelets but it also appears in a range of other tissues including smooth muscle cells and fibroblasts. The PDGF-B protein plays a substantial role in the regulation of growth and division inducing pathways important for cellular proliferation.
- Biological function summary
PDGF-B influences cellular signaling processes and facilitates interactions that are a part of the PDGF receptor system. PDGF-B can form a homo-dimerization commonly known as the PDGF-BB dimer which is an active form. When PDGF-B binds to its receptor specifically the PDGF receptor Β (PDGFR-Β) it triggers pathways that control various cellular activities including growth and survival. The interaction of PDGF-B with the receptor is an important step that modulates several downstream signaling pathways.
- Pathways
PDGF-B activates significant pathways such as the MAPK/ERK and PI3K/AKT pathways. These pathways play a major role in the regulation of cell proliferation and differentiation. Through these pathways PDGF-B exhibits interactions with proteins like Ras and Akt which are integral in transmitting signals within the cell. These cascades result in the promotion of processes such as angiogenesis and wound healing highlighting the importance of PDGF-B in cellular function.
- Associated diseases and disorders
Researchers have linked PDGF-B to pathologies such as cancer and atherosclerosis. For instance overexpression of PDGF-B can lead to abnormal vascular growth contributing to tumor development in cancers. It also plays a role in the progression of atherosclerosis by promoting the migration and proliferation of smooth muscle cells leading to plaque formation. In these contexts PDGF-B shows interaction with other proteins such as VEGF in tumor angiogenesis and LDL in atherosclerotic developments reinforcing its involvement in these conditions.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
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6 product images
This image is courtesy of a customer review submitted by Lauren CarpenterImmunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PDGF B antibody (ab23914)
ab23914 staining PDGF B (green) in mouse skeletal muscle tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 10% serum for 10 minutes at 25°C; antigen retrieval was by heat mediation in Tris pH 9. Samples were incubated with primary antibody (1/500 in antibody diluent reagent) for 14 hours at 4°C. An Alexa Fluor® 647-conjugated goat anti-rabbit IgG polyclonal (1/200) was used as the secondary antibody. DAPI (blue) - nuclei.
This image is courtesy of an anonymous customer reviewImmunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PDGF B antibody (ab23914)
ab23914 staining PDGF B (green) in Mouse skeletal muscle (gastrocnemius) tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with methacarn and blocked with 10% serum for 10 minutes at 25°C; antigen retrieval was by heat mediation in Tris pH 8. Samples were incubated with primary antibody (1/250 in antibody diluent reagent) for 14 hours at 4°C. An Alexa Fluor® 488-conjugated Goat anti-rabbit IgG polyclonal (1/200) was used as the secondary antibody. Red - WGA membranes, Blue - DAPI Nuclei.
Western blot - Anti-PDGF B antibody (ab23914)
ab23914 detects a band of ~ 14 kDa corresponding to the reduced form of recombinant PDGF B (Recombinant human PDGF B protein ab9706). The predicted molecular weight for PDGF B is 27 kDa according to the Swissprot entry, however the reduced form is known to run at between 14-18 kDa. The 14 kDa band is specifically blocked by the addition of the immunizing peptide, ab 24035.
All lanes: Western blot - Anti-PDGF B antibody (ab23914) at 1 µg/mL
Lane 1: Western blot - Recombinant human PDGF B protein (Recombinant human PDGF B protein ab9706) at 0.01 µg
Lane 2: Western blot - Recombinant human PDGF B protein (Recombinant human PDGF B protein ab9706) at 0.01 µg with Human PDGF B peptide (ab24035)
SecondaryAll lanes: Goat polyclonal to Rabbit IgG (Alexa Fluor® 680) at 1/10000 dilution
Performed under reducing conditions.
Predicted band size: 27 kDa
Observed band size: 14 kDa
Image courtesy of Human Protein AtlasImmunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PDGF B antibody (ab23914)
Image courtesy of Human Protein Atlas.
ab23914 staining PDGF B in Human kidney (male). The paraffin embedded tissue was incubated with ab23914 (1/500 dilution) for 30 mins at room temperature. Antigen retrieval was performed by heat induction in citrate buffer pH 6. ab23914 was tested in a tissue microarray (TMA) containing a wide range of normal and cancer tissues.
Western blot - Anti-PDGF B antibody (ab23914)
All lanes: Western blot - Anti-PDGF B antibody (ab23914) at 1 µg/mL
All lanes: Recombinant Human PDGF B protein (ab73231) at 0.01 µg
SecondaryAll lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (Goat Anti-Rabbit IgG H&L (HRP) preadsorbed ab97080) at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 27 kDa
Exposure time: 1min
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PDGF B antibody (ab23914)
IHC image of PDGF B staining in human heart formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab23914, 5μg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
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