Anti-PDGF B antibody

• IHC-P

AbReview40760****

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PDGF B antibody (AB23914)

ab23914 staining PDGF B (green) in mouse skeletal muscle tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 10% serum for 10 minutes at 25°C; antigen retrieval was by heat mediation in Tris pH 9. Samples were incubated with primary antibody (1/500 in antibody diluent reagent) for 14 hours at 4°C. An Alexa Fluor® 647-conjugated goat anti-rabbit IgG polyclonal (1/200) was used as the secondary antibody. DAPI (blue) - nuclei.

This image is courtesy of an Abreview submitted by Lauren Carpenter

• IHC-P

Lab

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PDGF B antibody (AB23914)

IHC image of PDGF B staining in human heart formalin fixed paraffin embedded tissue section, performed on a Leica Bond^TM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab23914, 5μg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

• IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PDGF B antibody (AB23914)

Image courtesy of Human Protein Atlas.

ab23914 staining PDGF B in Human kidney (male). The paraffin embedded tissue was incubated with ab23914 (1/500 dilution) for 30 mins at room temperature. Antigen retrieval was performed by heat induction in citrate buffer pH 6. ab23914 was tested in a tissue microarray (TMA) containing a wide range of normal and cancer tissues.

Image courtesy of Human Protein Atlas

• IHC-P

AbReview39244****

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PDGF B antibody (AB23914)

ab23914 staining PDGF B (green) in Mouse skeletal muscle (gastrocnemius) tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with methacarn and blocked with 10% serum for 10 minutes at 25°C; antigen retrieval was by heat mediation in Tris pH 8. Samples were incubated with primary antibody (1/250 in antibody diluent reagent) for 14 hours at 4°C. An Alexa Fluor® 488-conjugated Goat anti-rabbit IgG polyclonal (1/200) was used as the secondary antibody. Red - WGA membranes, Blue - DAPI Nuclei.

This image is courtesy of an anonymous Abreview

• WB

Project

Western blot - Anti-PDGF B antibody (AB23914)

ab23914 detects a band of ~ 14 kDa corresponding to the reduced form of recombinant PDGF B (ab9706). The predicted molecular weight for PDGF B is 27 kDa according to the Swissprot entry, however the reduced form is known to run at between 14-18 kDa. The 14 kDa band is specifically blocked by the addition of the immunizing peptide, ab 24035.

All lanes:

Western blot - Anti-PDGF B antibody (ab23914) at 1 µg/mL

Lane 1:

Western blot - Recombinant human PDGF B protein (<a href='/en-us/products/proteins-peptides/recombinant-human-pdgf-b-protein-ab9706'>ab9706</a>) at 0.01 µg

Lane 2:

Western blot - Recombinant human PDGF B protein (<a href='/en-us/products/proteins-peptides/recombinant-human-pdgf-b-protein-ab9706'>ab9706</a>) at 0.01 µg with Human PDGF B peptide (<a href='/en-us/products/unavailable/human-pdgf-b-peptide-ab24035'>ab24035</a>)

Secondary

All lanes:

Goat polyclonal to Rabbit IgG (Alexa Fluor® 680) at 1/10000 dilution

Predicted band size: 27 kDa

Observed band size: 14 kDa

false

• WB

Unknown

Western blot - Anti-PDGF B antibody (AB23914)

All lanes:

Western blot - Anti-PDGF B antibody (ab23914) at 1 µg/mL

All lanes:

Recombinant Human PDGF B protein (<a href='/en-us/products/unavailable/recombinant-human-pdgf-b-protein-ab73231'>ab73231</a>) at 0.01 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-preadsorbed-ab97080'>ab97080</a>) at 1/5000 dilution

Predicted band size: 27 kDa

true

Exposure time: 1min