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AB178409

Anti-PDGF B antibody [EPR6834]

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(29 Publications)

Anti-PDGF B antibody [EPR6834] (ab178409) is a rabbit monoclonal antibody detecting PDGF B in Western Blot. Suitable for Human, Mouse, Rat.

- Biophysical QC for unrivalled batch-batch consistency
- Over 10 publications

View Alternative Names

PDGF2, SIS, PDGFB, Platelet-derived growth factor subunit B, PDGF subunit B, PDGF-2, Platelet-derived growth factor B chain, Platelet-derived growth factor beta polypeptide, Proto-oncogene c-Sis

2 Images
Western blot - Anti-PDGF B antibody [EPR6834] (AB178409)
  • WB

Lab

Western blot - Anti-PDGF B antibody [EPR6834] (AB178409)

Blocking/Diluting Buffer and concentration : 5% NFDM/TBST

All lanes:

Western blot - Anti-PDGF B antibody [EPR6834] (ab178409) at 1/1000 dilution

Lane 1:

Mouse heart tissue lysate at 20 µg

Lane 2:

Rat heart tissue lysate at 20 µg

Lane 3:

Human brain tissue lysate at 20 µg

Lane 4:

Mouse brain tissue lysate at 20 µg

Lane 5:

Rat brain tissue lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Predicted band size: 27 kDa

Observed band size: 27 kDa

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Western blot - Anti-PDGF B antibody [EPR6834] (AB178409)
  • WB

Supplier Data

Western blot - Anti-PDGF B antibody [EPR6834] (AB178409)

All lanes:

Western blot - Anti-PDGF B antibody [EPR6834] (ab178409) at 1/1000 dilution

Lane 1:

Fetal brain tissue lysate at 10 µg

Lane 2:

A375 cell lysate at 10 µg

Lane 3:

HeLa cell lysate at 10 µg

Secondary

All lanes:

HRP labeled goat anti-rabbit at 1/2000 dilution

Predicted band size: 27 kDa

false

  • Carrier free

    Anti-PDGF B antibody [EPR6834] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR6834

Isotype

IgG

Carrier free

No

Reacts with

Mouse, Rat, Human

Applications

WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "1/50 - 1/100", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval before commencing with IHC staining protocol." }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "1/50 - 1/100", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval before commencing with IHC staining protocol." }, "Rat": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "1/50 - 1/100", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval before commencing with IHC staining protocol." }, "Sheep": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }
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Product details

What is this antibody validated in?
Anti-PDGF B antibody [EPR6834] (ab178409) is a rabbit recombinant monoclonal antibody and is validated for use in Western Blot (WB) in Human, Mouse, Rat samples.

What is the molecular weight of PDGF B?
Anti-PDGF B [EPR6834] (ab178409) specifically detects a band for PDGF B (UniProt: P01127) at a molecular weight of 27kDa.

Trusted by the scientific community
Anti-PDGF B [EPR6834] (ab178409) was first used in a scientific publication in 2013 and has been cited over 10 times in peer-reviewed journals.

Trial sizes available!
Test your antibody or perform pre-screening before committing to a larger quantity. Sold in 10µl. Discover our selection of trial-size antibodies.

Other related products
We have a range of other formats of antibody clone [EPR6834] also available for your convenience: ab178409, Carrier free - ab240207

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Shipped at conditions
Conditional Ambient
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

PDGF-B also known as platelet-derived growth factor subunit B or PDGF-BB is a critical protein involved in various cellular processes. Composed of 241 amino acids PDGF-B exhibits a molecular mass of approximately 27 kDa. Scientists recognize this protein for its expression primarily in platelets but it also appears in a range of other tissues including smooth muscle cells and fibroblasts. The PDGF-B protein plays a substantial role in the regulation of growth and division inducing pathways important for cellular proliferation.
Biological function summary

PDGF-B influences cellular signaling processes and facilitates interactions that are a part of the PDGF receptor system. PDGF-B can form a homo-dimerization commonly known as the PDGF-BB dimer which is an active form. When PDGF-B binds to its receptor specifically the PDGF receptor Β (PDGFR-Β) it triggers pathways that control various cellular activities including growth and survival. The interaction of PDGF-B with the receptor is an important step that modulates several downstream signaling pathways.

Pathways

PDGF-B activates significant pathways such as the MAPK/ERK and PI3K/AKT pathways. These pathways play a major role in the regulation of cell proliferation and differentiation. Through these pathways PDGF-B exhibits interactions with proteins like Ras and Akt which are integral in transmitting signals within the cell. These cascades result in the promotion of processes such as angiogenesis and wound healing highlighting the importance of PDGF-B in cellular function.

Researchers have linked PDGF-B to pathologies such as cancer and atherosclerosis. For instance overexpression of PDGF-B can lead to abnormal vascular growth contributing to tumor development in cancers. It also plays a role in the progression of atherosclerosis by promoting the migration and proliferation of smooth muscle cells leading to plaque formation. In these contexts PDGF-B shows interaction with other proteins such as VEGF in tumor angiogenesis and LDL in atherosclerotic developments reinforcing its involvement in these conditions.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Growth factor that plays an essential role in the regulation of embryonic development, cell proliferation, cell migration, survival and chemotaxis. Potent mitogen for cells of mesenchymal origin (PubMed : 26599395). Required for normal proliferation and recruitment of pericytes and vascular smooth muscle cells in the central nervous system, skin, lung, heart and placenta. Required for normal blood vessel development, and for normal development of kidney glomeruli. Plays an important role in wound healing. Signaling is modulated by the formation of heterodimers with PDGFA (By similarity).
See full target information PDGFB
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Publications (29)

Recent publications for all applications. Explore the full list and refine your search

Journal of cell communication and signaling 19:e70025 PubMed40520075

2025

Adipose-derived stem cell exosomes alleviate TGF-β1-induced urethral stricture fibrosis by suppressing the TGF-β/Smad pathway and downstream PDGFR-β/RAS/ERK signaling.

Applications

Unspecified application

Species

Unspecified reactive species

Tao Liang,Chao Deng,Hang Guo,Zhenghao Dai,Yiwen Jiang,Yuting Lu,Weiguo Chen

Frontiers in pharmacology 16:1548122 PubMed40365318

2025

Protective effect of brain microvascular endothelial cell-derived exosomes on blood-brain barrier induced by ischemia-reperfusion injury and .

Applications

Unspecified application

Species

Unspecified reactive species

Jin Sun,Meng Wang,Lichen Guo,Yushuang Cao,Linlin Su,Shaoxia Wang,Lijuan Chai,Qing Yuan,Limin Hu

Stem cell reviews and reports 21:834-848 PubMed39871082

2025

hUC-MSCs Prevent Acute High-Altitude Injury through Apoe/Pdgf-b/p-Erk1/2 Axis in Mice.

Applications

Unspecified application

Species

Unspecified reactive species

Siyu Yan,Youkun Bi,Qun Liu,Shaole Song,Lihong Ma,Guangju Ji

International journal of biological sciences 21:802-822 PubMed39781468

2025

Tubulointerstitial nephritis antigen-like 1 promotes the progression of liver fibrosis after HCV eradication with direct-acting antivirals.

Applications

Unspecified application

Species

Unspecified reactive species

Lei Lei,Hu Li,Xue-Kai Wang,Jian-Rui Li,Han Sun,Hong-Ying Li,Jia-Yu Li,Mei Tang,Jing-Chen Xu,Biao Dong,Yue Gong,Dan-Qing Song,Jian-Dong Jiang,Zong-Gen Peng

Journal of cellular and molecular medicine 28:e70293 PubMed39720896

2024

Single-Cell RNA-Seq Reveals Injuries in Aortic Dissection and Identifies PDGF Signalling Pathway as a Potential Therapeutic Target.

Applications

Unspecified application

Species

Unspecified reactive species

Yichi Han,Yongji Cui,Juli Liu,Dingchen Wang,Guoxiang Zou,Xin Qi,Jinxiu Meng,Xiaoran Huang,Haiwei He,Xin Li

PloS one 19:e0301199 PubMed39172956

2024

Blocking IL-33 decelerates cartilage degeneration in knee osteoarthritis through mice model.

Applications

Unspecified application

Species

Unspecified reactive species

Fan Wu,Siyuan Zhang,Rujie Zhuang,Chuanxiao Hu,Kangxiang Zhu

Molecular medicine reports 29: PubMed38488028

2024

Pigment epithelium‑derived factor inhibits proliferation, invasion and angiogenesis, and induces ferroptosis of extravillous trophoblasts by targeting Wnt‑β‑catenin/VEGF signaling  in placenta accreta spectrum.

Applications

Unspecified application

Species

Unspecified reactive species

Rui Li,Xiaoying Weng,Xuyang Hu,Junjun Wang,Lin Zheng

Frontiers in pharmacology 15:1341020 PubMed38469403

2024

Quantitative proteomics reveals the protective effects of Yinchenzhufu decoction against cholestatic liver fibrosis in mice by inhibiting the PDGFRβ/PI3K/AKT pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Qian Meng,Hongwen Zhu,Yuanyuan Li,Xiaotian Peng,Tianming Wang,Hui Huang,Hu Zhou,Yuejia Liu,Sujie Ru,Jiasheng Wu,Yueming Ma

iScience 26:108236 PubMed37953957

2023

Exosomes derived from platelet-rich plasma promote diabetic wound healing via the JAK2/STAT3 pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Wenhai Cao,Xiaotong Meng,Fangming Cao,Jinpeng Wang,Maowei Yang

International journal of molecular medicine 52: PubMed37800598

2023

Flavin containing monooxygenase 2 regulates renal tubular cell fibrosis and paracrine secretion via SMURF2 in AKI‑CKD transformation.

Applications

Unspecified application

Species

Unspecified reactive species

Longfei Wang,Hongchu Zha,Jing Huang,Lang Shi
View all publications
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

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