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AB9704

Anti-PDGF BB antibody

4

(2 Reviews)

|

(9 Publications)

Rabbit Polyclonal PDGF B antibody. Suitable for WB and reacts with Human samples. Cited in 9 publications. Immunogen corresponding to Recombinant Full Length Protein corresponding to Human PDGFB.

View Alternative Names

PDGF2, SIS, PDGFB, Platelet-derived growth factor subunit B, PDGF subunit B, PDGF-2, Platelet-derived growth factor B chain, Platelet-derived growth factor beta polypeptide, Proto-oncogene c-Sis

1 Images
Western blot - Anti-PDGF BB antibody (AB9704)
  • WB

Unknown

Western blot - Anti-PDGF BB antibody (AB9704)

The 29kDa form is the propeptide.

All lanes:

Western blot - Anti-PDGF BB antibody (ab9704) at 0.2 µg/mL

All lanes:

A-431 (Human) Whole Cell Lysate (ab52255) at 10 µg

Secondary

All lanes:

Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution

Predicted band size: 27 kDa

Observed band size: 18 kDa,29 kDa,45 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

Unknown

Light chain type

unknown

Carrier free

No

Reacts with

Human

Applications

WB

applications

Immunogen

Recombinant Full Length Protein corresponding to Human PDGFB.

P01127

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Alternative Products

Primary Antibodies

AB178409

Anti-PDGF B antibody [EPR6834]

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Western blot - Anti-PDGF B antibody [EPR6834] (AB178409)

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Primary Antibodies

AB23914

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PDGF B antibody (AB23914)

  • 4
  • 12
Primary Antibodies

AB240207

Anti-PDGF B antibody [EPR6834] - BSA and Azide free

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Western blot - Anti-PDGF B antibody [EPR6834] - BSA and Azide free (AB240207)

  • 0
  • 0

Complementary Products

Secondary Antibodies

AB150077

Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488)

Immunocytochemistry/ Immunofluorescence - Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (AB150077)

  • 5
  • 20
Primary Antibodies

AB32570

Anti-PDGFR alpha + PDGFR beta antibody [Y92] - C-terminal

BOND RX™ Validated|RabMAb|Recombinant|KO Validated

Western blot - Anti-PDGFR alpha + PDGFR beta antibody [Y92] - C-terminal (AB32570)

  • 4
  • 31
Primary Antibodies

AB9485

Anti-GAPDH antibody - Loading Control

BOND RX™ Validated|Lab Essentials

Immunocytochemistry/ Immunofluorescence - Anti-GAPDH antibody - Loading Control (AB9485)

  • 5
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Primary Antibodies

AB7817

Anti-alpha smooth muscle Actin antibody [1A4]

KO Validated

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-alpha smooth muscle Actin antibody [1A4] (AB7817)

  • 5
  • 105
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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p>To detect hPDGF-BB by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 µg/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant hPDGF-BB is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.</p>" } } }
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Product details

This product is no longer batch tested in IHC, for an IHC validated antibody please see ab107101
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Properties and storage information

Form
Lyophilized
Reconstitution
reconstitute with water at 200µL
Purification technique
Affinity purification Immunogen
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

PDGF-B also known as platelet-derived growth factor subunit B or PDGF-BB is a critical protein involved in various cellular processes. Composed of 241 amino acids PDGF-B exhibits a molecular mass of approximately 27 kDa. Scientists recognize this protein for its expression primarily in platelets but it also appears in a range of other tissues including smooth muscle cells and fibroblasts. The PDGF-B protein plays a substantial role in the regulation of growth and division inducing pathways important for cellular proliferation.
Biological function summary

PDGF-B influences cellular signaling processes and facilitates interactions that are a part of the PDGF receptor system. PDGF-B can form a homo-dimerization commonly known as the PDGF-BB dimer which is an active form. When PDGF-B binds to its receptor specifically the PDGF receptor β (PDGFR-β) it triggers pathways that control various cellular activities including growth and survival. The interaction of PDGF-B with the receptor is an important step that modulates several downstream signaling pathways.

Pathways

PDGF-B activates significant pathways such as the MAPK/ERK and PI3K/AKT pathways. These pathways play a major role in the regulation of cell proliferation and differentiation. Through these pathways PDGF-B exhibits interactions with proteins like Ras and Akt which are integral in transmitting signals within the cell. These cascades result in the promotion of processes such as angiogenesis and wound healing highlighting the importance of PDGF-B in cellular function.

Researchers have linked PDGF-B to pathologies such as cancer and atherosclerosis. For instance overexpression of PDGF-B can lead to abnormal vascular growth contributing to tumor development in cancers. It also plays a role in the progression of atherosclerosis by promoting the migration and proliferation of smooth muscle cells leading to plaque formation. In these contexts PDGF-B shows interaction with other proteins such as VEGF in tumor angiogenesis and LDL in atherosclerotic developments reinforcing its involvement in these conditions.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Growth factor that plays an essential role in the regulation of embryonic development, cell proliferation, cell migration, survival and chemotaxis. Potent mitogen for cells of mesenchymal origin (PubMed : 26599395). Required for normal proliferation and recruitment of pericytes and vascular smooth muscle cells in the central nervous system, skin, lung, heart and placenta. Required for normal blood vessel development, and for normal development of kidney glomeruli. Plays an important role in wound healing. Signaling is modulated by the formation of heterodimers with PDGFA (By similarity).
See full target information PDGFB
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Publications (9)

Recent publications for all applications. Explore the full list and refine your search

Cell death discovery 8:160 PubMed35379779

2022

Smurf2-induced degradation of SMAD2 causes inhibition of hair follicle stem cell differentiation.

Applications

Unspecified application

Species

Unspecified reactive species

Bojie Lin,Dan Huang,Guanyu Lin,Yong Miao,Jin Wang,Zhexiang Fan,Zhiqi Hu

PloS one 16:e0260130 PubMed34965258

2021

The involvement of TGF-β1 /FAK/α-SMA pathway in the antifibrotic impact of rice bran oil on thioacetamide-induced liver fibrosis in rats.

Applications

Unspecified application

Species

Unspecified reactive species

Rehab F Abdel-Rahman,Hany M Fayed,Gihan F Asaad,Hanan A Ogaly,Alyaa F Hessin,Abeer A A Salama,Sahar S Abd El-Rahman,Mahmoud S Arbid,Marawan Abd Elbaset Mohamed

Stem cell research & therapy 11:444 PubMed33076979

2020

DNMT1-mediated methylation inhibits microRNA-214-3p and promotes hair follicle stem cell differentiate into adipogenic lineages.

Applications

Unspecified application

Species

Unspecified reactive species

Fangcao Jin,Min Li,Xuyang Li,Yunpeng Zheng,Kun Zhang,Xiaojun Liu,Bingjie Cai,Guangwen Yin

Molecular medicine reports 22:886-894 PubMed32467985

2020

TIM‑3 inhibits PDGF‑BB‑induced atherogenic responses in human artery vascular smooth muscle cells.

Applications

Unspecified application

Species

Unspecified reactive species

Chong Lian,Zhecun Wang,Jiacong Qiu,Baohong Jiang,Junbing Lv,Rongzhou He,Ruiming Liu,Wen Li,Jinsong Wang,Shenming Wang

Scientific reports 6:19404 PubMed26762853

2016

Endothelial Cords Promote Tumor Initial Growth prior to Vascular Function through a Paracrine Mechanism.

Applications

Unspecified application

Species

Unspecified reactive species

Chengjian Zhao,Wei Zhang,Yuwei Zhao,Yun Yang,Hui Luo,Gaili Ji,E Dong,Hongxing Deng,Shuo Lin,Yuquan Wei,Hanshuo Yang

Electrophoresis 36:2017-26 PubMed26010067

2015

Exosome enrichment of human serum using multiple cycles of centrifugation.

Applications

Unspecified application

Species

Unspecified reactive species

Jeongkwon Kim,Zhijing Tan,David M Lubman

The European respiratory journal 37:968-70 PubMed21454902

2011

An autopsy case of pulmonary veno-occlusive disease refractory to imatinib.

Applications

Unspecified application

Species

Unspecified reactive species

H Koiwa,I Tsujino,D Ikeda,H Ohira,M Tanino,M Nishimura

Biosensors & bioelectronics 25:1717-22 PubMed20047825

2010

Electrochemical detection of vascular endothelial growth factors (VEGFs) using VEGF antibody fragments modified Au NPs/ITO electrode.

Applications

Unspecified application

Species

Unspecified reactive species

Gang-Il Kim,Kyung-Woo Kim,Min-Kyu Oh,Yun-Mo Sung

Blood 115:51-60 PubMed19880494

2009

Platelet-derived growth factor mediates survival of leukemic large granular lymphocytes via an autocrine regulatory pathway.

Applications

Neut

Species

Human

Jun Yang,Xin Liu,Susan B Nyland,Ranran Zhang,Lindsay K Ryland,Kathleen Broeg,Kendall Thomas Baab,Nancy Ruth Jarbadan,Rosalyn Irby,Thomas P Loughran
View all publications
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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