Rabbit Polyclonal PDGF B antibody. Suitable for WB and reacts with Human samples. Cited in 9 publications. Immunogen corresponding to Recombinant Full Length Protein corresponding to Human PDGFB.
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- Cell death discovery 8:1602022Smurf2-induced degradation of SMAD2 causes inhibition of hair follicle stem cell differentiation.Applications:Unspecified applicationReactive species:Unspecified reactive speciesBojie Lin,Dan Huang,Guanyu Lin,Yong Miao,Jin Wang,Zhexiang Fan,Zhiqi HuPubMed 35379779
- PloS one 16:e02601302021The involvement of TGF-β1 /FAK/α-SMA pathway in the antifibrotic impact of rice bran oil on thioacetamide-induced liver fibrosis in rats.Applications:Unspecified applicationReactive species:Unspecified reactive speciesRehab F Abdel-Rahman,Hany M Fayed,Gihan F Asaad,Hanan A Ogaly,Alyaa F Hessin,Abeer A A Salama,Sahar S Abd El-Rahman,Mahmoud S Arbid,Marawan Abd Elbaset MohamedPubMed 34965258
- Stem cell research & therapy 11:4442020DNMT1-mediated methylation inhibits microRNA-214-3p and promotes hair follicle stem cell differentiate into adipogenic lineages.Applications:Unspecified applicationReactive species:Unspecified reactive speciesFangcao Jin et. al.PubMed 33076979
- Molecular medicine reports 22:886-8942020TIM‑3 inhibits PDGF‑BB‑induced atherogenic responses in human artery vascular smooth muscle cells.Applications:Unspecified applicationReactive species:Unspecified reactive speciesChong Lian et. al.PubMed 32467985
- Scientific reports 6:194042016Endothelial Cords Promote Tumor Initial Growth prior to Vascular Function through a Paracrine Mechanism.Applications:Unspecified applicationReactive species:Unspecified reactive speciesChengjian Zhao et. al.PubMed 26762853
- Electrophoresis 36:2017-262015Exosome enrichment of human serum using multiple cycles of centrifugation.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJeongkwon Kim et. al.PubMed 26010067
- The European respiratory journal 37:968-702011An autopsy case of pulmonary veno-occlusive disease refractory to imatinib.Applications:Unspecified applicationReactive species:Unspecified reactive speciesH Koiwa,I Tsujino,D Ikeda,H Ohira,M Tanino,M NishimuraPubMed 21454902
- Biosensors & bioelectronics 25:1717-222010Electrochemical detection of vascular endothelial growth factors (VEGFs) using VEGF antibody fragments modified Au NPs/ITO electrode.Applications:Unspecified applicationReactive species:Unspecified reactive speciesGang-Il Kim,Kyung-Woo Kim,Min-Kyu Oh,Yun-Mo SungPubMed 20047825
- Blood 115:51-602009Platelet-derived growth factor mediates survival of leukemic large granular lymphocytes via an autocrine regulatory pathway.Applications:NeutReactive species:HumanJun Yang,Xin Liu,Susan B Nyland,Ranran Zhang,Lindsay K Ryland,Kathleen Broeg,Kendall Thomas Baab,Nancy Ruth Jarbadan,Rosalyn Irby,Thomas P LoughranPubMed 19880494
Key facts
- Isotype
- Unknown
- Host species
- Rabbit
- Storage buffer
- Standard buffer
- Form
- Lyophilized
- Clonality
- Polyclonal
Immunogen
- Recombinant Full Length Protein corresponding to Human PDGFB. Database link P01127
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Reactivity data
Select an application| WB | |
|---|---|
| Human | Tested |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes To detect hPDGF-BB by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 µg/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant hPDGF-BB is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions. |
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Target data
Function
Growth factor that plays an essential role in the regulation of embryonic development, cell proliferation, cell migration, survival and chemotaxis. Potent mitogen for cells of mesenchymal origin (PubMed:26599395). Required for normal proliferation and recruitment of pericytes and vascular smooth muscle cells in the central nervous system, skin, lung, heart and placenta. Required for normal blood vessel development, and for normal development of kidney glomeruli. Plays an important role in wound healing. Signaling is modulated by the formation of heterodimers with PDGFA (By similarity).
Alternative names
PDGF2, SIS, PDGFB, Platelet-derived growth factor subunit B, PDGF subunit B, PDGF-2, Platelet-derived growth factor B chain, Platelet-derived growth factor beta polypeptide, Proto-oncogene c-Sis
Recommended products
Rabbit Polyclonal PDGF B antibody. Suitable for WB and reacts with Human samples. Cited in 9 publications. Immunogen corresponding to Recombinant Full Length Protein corresponding to Human PDGFB.
Key facts
- Isotype
- Unknown
- Host species
- Rabbit
- Storage buffer
- Standard buffer
- Form
- Lyophilized
- Clonality
- Polyclonal
- Immunogen
- Recombinant Full Length Protein corresponding to Human PDGFB. Database link P01127
- Purification technique
- Affinity purification Immunogen
- Light chain type
- unknown
- Reconstitution
- reconstitute with water at 200µL
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
Notes
This product is no longer batch tested in IHC, for an IHC validated antibody please see Anti-PDGF B antibody [326A] ab107101
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
PDGF-B also known as platelet-derived growth factor subunit B or PDGF-BB is a critical protein involved in various cellular processes. Composed of 241 amino acids PDGF-B exhibits a molecular mass of approximately 27 kDa. Scientists recognize this protein for its expression primarily in platelets but it also appears in a range of other tissues including smooth muscle cells and fibroblasts. The PDGF-B protein plays a substantial role in the regulation of growth and division inducing pathways important for cellular proliferation.
- Biological function summary
PDGF-B influences cellular signaling processes and facilitates interactions that are a part of the PDGF receptor system. PDGF-B can form a homo-dimerization commonly known as the PDGF-BB dimer which is an active form. When PDGF-B binds to its receptor specifically the PDGF receptor β (PDGFR-β) it triggers pathways that control various cellular activities including growth and survival. The interaction of PDGF-B with the receptor is an important step that modulates several downstream signaling pathways.
- Pathways
PDGF-B activates significant pathways such as the MAPK/ERK and PI3K/AKT pathways. These pathways play a major role in the regulation of cell proliferation and differentiation. Through these pathways PDGF-B exhibits interactions with proteins like Ras and Akt which are integral in transmitting signals within the cell. These cascades result in the promotion of processes such as angiogenesis and wound healing highlighting the importance of PDGF-B in cellular function.
- Associated diseases and disorders
Researchers have linked PDGF-B to pathologies such as cancer and atherosclerosis. For instance overexpression of PDGF-B can lead to abnormal vascular growth contributing to tumor development in cancers. It also plays a role in the progression of atherosclerosis by promoting the migration and proliferation of smooth muscle cells leading to plaque formation. In these contexts PDGF-B shows interaction with other proteins such as VEGF in tumor angiogenesis and LDL in atherosclerotic developments reinforcing its involvement in these conditions.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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1 product image
Western blot - Anti-PDGF BB antibody (ab9704)
The 29kDa form is the propeptide.
All lanes: Western blot - Anti-PDGF BB antibody (ab9704) at 0.2 µg/mL
All lanes: A-431 (Human) Whole Cell Lysate (ab52255) at 10 µg
SecondaryAll lanes: Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Predicted band size: 27 kDa
Observed band size: 18 kDa, 29 kDa, 45 kDa
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