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AB325049

Anti-PDGFA antibody [EPR29493-7] - BSA and Azide free (Detector)

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Rabbit Recombinant Monoclonal PDGF-AA antibody. Carrier free. Suitable for sELISA and reacts with Mouse samples.

View Alternative Names

Platelet-derived growth factor subunit A, PDGF subunit A, PDGF-1, Platelet-derived growth factor A chain, Platelet-derived growth factor alpha polypeptide, Pdgfa

3 Images
Sandwich ELISA - Anti-PDGFA antibody [EPR29493-7] - BSA and Azide free (Detector) (AB325049)
  • sELISA

Supplier Data

Sandwich ELISA - Anti-PDGFA antibody [EPR29493-7] - BSA and Azide free (Detector) (AB325049)

Interpolated concentration of native PDGFA was measured at different sample concentrations. Undiluted sample is as follows : Mouse placenta extract (1mg/ml) . The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). Sample dilutions are made in 1% bovine serum albumin (BSA). Data represented here are indicative of assay working using example of biological sample. However, we do not guarantee same sensitivity level due to biological variation.

Sandwich ELISA - Anti-PDGFA antibody [EPR29493-7] - BSA and Azide free (Detector) (AB325049)
  • sELISA

Supplier Data

Sandwich ELISA - Anti-PDGFA antibody [EPR29493-7] - BSA and Azide free (Detector) (AB325049)

Interpolated concentration of native PDGFA was measured at different sample concentrations. Undiluted sample is as follows : Mouse serum 100%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2) Sample dilutions are made in 1% bovine serum albumin (BSA). Data represented here are indicative of assay working using example of biological sample. However, we do not guarantee same sensitivity level due to biological variation.

Sandwich ELISA - Anti-PDGFA antibody [EPR29493-7] - BSA and Azide free (Detector) (AB325049)
  • sELISA

Supplier Data

Sandwich ELISA - Anti-PDGFA antibody [EPR29493-7] - BSA and Azide free (Detector) (AB325049)

Sandwich ELISA using Mouse PDGFA immunogen protein standard curve with the capture antibody ab325048 at 2 µg/mL and detector antibody ab325049 at 0.5 µg/mL. Background-subtracted absorbance data values (mean +/- standard deviation are shown.)

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR29493-7

Isotype

IgG

Carrier free

Yes

Reacts with

Mouse

Applications

sELISA

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

Specificity

Clone is specific to mouse PDGF A.

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Reactivity data

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Product details

ab325049 is a BSA and Azide Free antibody supplied in an unconjugated format and it is suitable for sandwich ELISAs to quantify Mouse PDGFA. The recommended pair for sandwich ELISA is:
Capture: ab325048, Mouse PDGFA Capture Antibody (unconjugated)
Detector: ab325049, Mouse PDGFA Detector Antibody (unconjugated)
The reference range value is 78.13-10000 pg/mL.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Sandwich ELISA
The recommended antibody orientation is based on internal optimization for ELISA-based assays. Antibody orientation is assay dependent and needs to be optimized for each assay type. Please note that the range provided for this antibody is only an estimation based on the performance of the product using the recommended antibody pair. Performance of the antibody pair will depend on the specific characteristics of your assay. We guarantee the product works in sandwich ELISA, but we do not guarantee the sensitivity or dynamic range of the antibody in your assay.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 - 7.4 Constituents: 100% PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
Do Not Freeze
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Growth factor that plays an essential role in the regulation of embryonic development, cell proliferation, cell migration, survival and chemotaxis. Potent mitogen for cells of mesenchymal origin. Required for normal lung alveolar septum formation during embryogenesis, normal development of the gastrointestinal tract, normal development of Leydig cells and spermatogenesis. Required for normal oligodendrocyte development and normal myelination in the spinal cord and cerebellum. Plays an important role in wound healing. Signaling is modulated by the formation of heterodimers with PDGFB.
See full target information Pdgfa
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions
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Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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