Rat Monoclonal PDGFR alpha antibody. Carrier free. Suitable for Flow Cyt and reacts with Mouse samples. Immunogen corresponding to Recombinant Fragment Protein within Mouse Pdgfra.
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![Flow Cytometry - Anti-PDGFR alpha antibody [APA5] - Low endotoxin, Azide free (AB171230), expandable thumbnail](https://content.abcam.com/products/images/pdgfr-alpha-antibody-apa5-low-endotoxin-azide-free-ab171230--flow-cytometry-img90398.gif "Flow Cytometry - Anti-PDGFR alpha antibody [APA5] - Low endotoxin, Azide free (AB171230)")
Key facts
- Isotype
- IgG2a
- Host species
- Rat
- Storage buffer
pH: 7.2
Constituents: PBS- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- Recombinant Fragment Protein within Mouse Pdgfra. The exact immunogen used to generate this antibody is proprietary information. Database link P26618
Reactivity data
Select an application| Flow Cyt | |
|---|---|
| Mouse | Tested |
Flow Cyt
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info 1 µg for 105-108 Cells | Notes Final test volume = 100 μL ab18450 - Rat monoclonal IgG2a, is suitable for use as an isotype control with this antibody. |
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Target data
Function
Tyrosine-protein kinase that acts as a cell-surface receptor for PDGFA, PDGFB and PDGFC and plays an essential role in the regulation of embryonic development, cell proliferation, survival and chemotaxis. Depending on the context, promotes or inhibits cell proliferation and cell migration. Plays an important role in the differentiation of bone marrow-derived mesenchymal stem cells. Required for normal skeleton development and cephalic closure during embryonic development. Required for normal development of the mucosa lining the gastrointestinal tract, and for recruitment of mesenchymal cells and normal development of intestinal villi. Plays a role in cell migration and chemotaxis in wound healing. Plays a role in platelet activation, secretion of agonists from platelet granules, and in thrombin-induced platelet aggregation. Binding of its cognate ligands - homodimeric PDGFA, homodimeric PDGFB, heterodimers formed by PDGFA and PDGFB or homodimeric PDGFC -leads to the activation of several signaling cascades; the response depends on the nature of the bound ligand and is modulated by the formation of heterodimers between PDGFRA and PDGFRB. Phosphorylates PIK3R1, PLCG1, and PTPN11. Activation of PLCG1 leads to the production of the cellular signaling molecules diacylglycerol and inositol 1,4,5-trisphosphate, mobilization of cytosolic Ca(2+) and the activation of protein kinase C. Phosphorylates PIK3R1, the regulatory subunit of phosphatidylinositol 3-kinase, and thereby mediates activation of the AKT1 signaling pathway. Mediates activation of HRAS and of the MAP kinases MAPK1/ERK2 and/or MAPK3/ERK1. Promotes activation of STAT family members STAT1, STAT3 and STAT5A and/or STAT5B. Receptor signaling is down-regulated by protein phosphatases that dephosphorylate the receptor and its down-stream effectors, and by rapid internalization of the activated receptor.
Alternative names
CD140a, Platelet-derived growth factor receptor alpha, PDGF-R-alpha, PDGFR-alpha, Alpha platelet-derived growth factor receptor, Alpha-type platelet-derived growth factor receptor, CD140 antigen-like family member A, Platelet-derived growth factor alpha receptor, Pdgfra
Recommended products
Rat Monoclonal PDGFR alpha antibody. Carrier free. Suitable for Flow Cyt and reacts with Mouse samples. Immunogen corresponding to Recombinant Fragment Protein within Mouse Pdgfra.
Key facts
- Isotype
- IgG2a
- Host species
- Rat
- Storage buffer
pH: 7.2
Constituents: PBS- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- Recombinant Fragment Protein within Mouse Pdgfra. The exact immunogen used to generate this antibody is proprietary information. Database link P26618
- Carrier free
- Yes
- Clone number
- APA5
- Purification technique
- Affinity purification
- Light chain type
- kappa
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
Endotoxin Level: Less than 0.001 ng/μg antibody, as determined by the LAL assay.
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Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
PDGFR alpha also known as platelet-derived growth factor receptor alpha is a cell surface receptor tyrosine kinase with a mass of approximately 123 kDa. It plays a mechanical role in transmitting signals across the cell membrane initiating cellular responses. PDGFR alpha is widely expressed in mesenchymal cells including fibroblasts and smooth muscle cells. Researchers frequently employ techniques such as PDGFRA IHC and PDGFRA staining to study the localization and expression levels in tissues.
- Biological function summary
PDGFR alpha mediates cell proliferation survival and differentiation especially in embryonic development and wound healing. The receptor often forms a complex by dimerizing with itself or with PDGFR beta to activate intracellular signaling cascades. This process is initiated upon ligand binding which triggers autophosphorylation on tyrosine residues. The receptor is also recognized in the detection of PDGFRA with assays such as PDGFRA ELISA.
- Pathways
PDGFR alpha significantly affects the MAPK and PI3K/AKT signaling pathways which regulate cellular growth and survival signals. Through these pathways PDGFR alpha interacts with related proteins like SHP-2 and PI3K contributing to signal transduction involved in cellular responses. This role links PDGFR alpha to important cellular processes via its kinase activity and signal integration.
- Associated diseases and disorders
PDGFR alpha is implicated in conditions such as gastrointestinal stromal tumors (GISTs) and idiopathic pulmonary fibrosis (IPF). It connects to proteins such as KIT in GISTs where mutations cause aberrant receptor signaling leading to tumorigenesis. Aberrant PDGFR alpha signaling also associates with IPF a disorder characterized by increased fibroblast proliferation and fibrotic tissue formation. These connections make PDGFR alpha a significant target for research and therapeutic intervention.
Product promise
Tested
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Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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1 product image
Flow Cytometry - Anti-PDGFR alpha antibody [APA5] - Low endotoxin, Azide free (ab171230)
Flow cytometry analysis of NIH 3T3 cells using ab171230 at 0.5 μg (blue histogram) or purified Rat IgG2a kappa Isotype Control (open histogram) at 0.5 μg; Followed by anti-Rat IgG Biotin and Streptavidin PE. Total viable cells were used for analysis.
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