AB314105

Anti-PDGFR alpha antibody [BLR128H] - BSA free

Recombinant
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Rabbit Recombinant Monoclonal PDGFR alpha antibody. Suitable for ICC, IP, WB and reacts with Mouse, Human samples. Immunogen corresponding to Recombinant Fragment Protein within Human PDGFRA aa 1-550.

View Alternative Names

CD140a, PDGFR2, RHEPDGFRA, PDGFRA, Platelet-derived growth factor receptor alpha, PDGF-R-alpha, PDGFR-alpha, Alpha platelet-derived growth factor receptor, Alpha-type platelet-derived growth factor receptor, CD140 antigen-like family member A, CD140a antigen, Platelet-derived growth factor alpha receptor, Platelet-derived growth factor receptor 2, PDGFR-2

5 Images

Supplier Data

Immunocytochemistry - Anti-PDGFR alpha antibody [BLR128H] - BSA free (AB314105)

Detection of human PDGFR alpha in FFPE NCI-H1703 cells by immunocytochemistry. Antibody : Rabbit anti-PDGFR alpha recombinant monoclonal antibody [BLR128H] (A700-128). Secondary : HRP-conjugated goat antirabbit IgG. Substrate : DAB.

Supplier Data

Immunoprecipitation - Anti-PDGFR alpha antibody [BLR128H] - BSA free (AB314105)

Samples : Whole cell lysate (1 mg for IP; 5% of IP loaded) from NCI-H1703 cells prepared using NETN lysis buffer. Antibodies : Rabbit anti-PDGFR alpha recombinant monoclonal antibody [BLR128H] (A700-128) used for IP at 20 µl/mg lysate. PDGFR alpha was also immunoprecipitated by rabbit anti-PDGFR alpha antibody BL-1170-1G6. For blotting immunoprecipitated PDGFR alpha, A700-128 was used at 1 : 1000. Chemiluminescence with an exposure time of 75 seconds.

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Supplier Data

Immunocytochemistry - Anti-PDGFR alpha antibody [BLR128H] - BSA free (AB314105)

Detection of mouse PDGFR alpha in FFPE 3T3 cells by immunocytochemistry Antibody : Rabbit anti-PDGFR alpha recombinant monoclonal antibody [BLR128H] (A700-128). Secondary : HRP-conjugated goat anti-rabbit IgG. Substrate : DAB.

Supplier Data

Western blot - Anti-PDGFR alpha antibody [BLR128H] - BSA free (AB314105)

Samples : Whole cell lysate (50 µg) from NCI-H1703, HeLa, IMR-90, HEK293T, and Jurkat cells prepared using NETN lysis buffer. Antibody : Rabbit anti-PDGFR alpha recombinant monoclonal antibody [BLR128H] (A700-128) used at 1 : 1000. Secondary : HRP-conjugated goat anti-rabbit IgG. Chemiluminescence with an exposure time of 30 seconds. Lower Panel : Rabbit antiCOPB2 antibody.

All lanes:

Western blot - Anti-PDGFR alpha antibody [BLR128H] - BSA free (ab314105) at 1/1000 dilution

Lane 1:

NCI-H1703 Whole cell lysate at 50 µg

Lane 2:

HeLa Whole cell lysate at 50 µg

Lane 3:

IMR-90 Whole cell lysate at 50 µg

Lane 4:

HEK293T Whole cell lysate at 50 µg

Lane 5:

Jurkat Whole cell lysate at 50 µg

Secondary

All lanes:

HRP-conjugated goat anti-rabbit IgG

false

Supplier Data

Western blot - Anti-PDGFR alpha antibody [BLR128H] - BSA free (AB314105)

Samples : Whole cell lysate (50 µg) from mIMCD-3, A20, NIH 3T3, CT26, and CH27 cells prepared using NETN lysis buffer. Antibody : Rabbit anti-PDGFR alpha recombinant monoclonal antibody [BLR128H] (A700-128) used at 1 : 1000. Secondary : HRP-conjugated goat anti-rabbit IgG. Chemiluminescence with an exposure time of 3 minutes. Lower Panel : Rabbit anti-COPB2 antibody.

All lanes:

Western blot - Anti-PDGFR alpha antibody [BLR128H] - BSA free (ab314105) at 1/1000 dilution

Lane 1:

mIMCD-3 Whole cell lysate at 50 µg

Lane 2:

A20 Whole cell lysate at 50 µg

Lane 3:

NIH 3T3 Whole cell lysate at 50 µg

Lane 4:

CT26 Whole cell lysate at 50 µg

Lane 5:

CH27 Whole cell lysate at 50 µg

Secondary

All lanes:

HRP-conjugated goat anti-rabbit IgG

false

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

BLR128H

Isotype

IgG

Carrier free

Reacts with

Human, Mouse

Applications

IP, ICC, WB

applications

Immunogen

Recombinant Fragment Protein within Human PDGFRA aa 1-550. The exact immunogen used to generate this antibody is proprietary information.

P16234

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICC" : {"fullname" : "Immunocytochemistry", "shortname":"ICC"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ICC-species-checked": "testedAndGuaranteed", "ICC-species-dilution-info": "", "ICC-species-notes": "", "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Mouse": { "ICC-species-checked": "testedAndGuaranteed", "ICC-species-dilution-info": "", "ICC-species-notes": "", "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "" } } }

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Product details

REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

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Properties and storage information

Form

Liquid

Storage buffer

pH: 7.8 - 8.6 Preservative: 0.09% Sodium azide Constituents: 98% Borate buffered saline

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

Up to 12 months

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

PDGFR alpha also known as platelet-derived growth factor receptor alpha is a cell surface receptor tyrosine kinase with a mass of approximately 123 kDa. It plays a mechanical role in transmitting signals across the cell membrane initiating cellular responses. PDGFR alpha is widely expressed in mesenchymal cells including fibroblasts and smooth muscle cells. Researchers frequently employ techniques such as PDGFRA IHC and PDGFRA staining to study the localization and expression levels in tissues.

Biological function summary

PDGFR alpha mediates cell proliferation survival and differentiation especially in embryonic development and wound healing. The receptor often forms a complex by dimerizing with itself or with PDGFR beta to activate intracellular signaling cascades. This process is initiated upon ligand binding which triggers autophosphorylation on tyrosine residues. The receptor is also recognized in the detection of PDGFRA with assays such as PDGFRA ELISA.

Pathways

PDGFR alpha significantly affects the MAPK and PI3K/AKT signaling pathways which regulate cellular growth and survival signals. Through these pathways PDGFR alpha interacts with related proteins like SHP-2 and PI3K contributing to signal transduction involved in cellular responses. This role links PDGFR alpha to important cellular processes via its kinase activity and signal integration.

PDGFR alpha is implicated in conditions such as gastrointestinal stromal tumors (GISTs) and idiopathic pulmonary fibrosis (IPF). It connects to proteins such as KIT in GISTs where mutations cause aberrant receptor signaling leading to tumorigenesis. Aberrant PDGFR alpha signaling also associates with IPF a disorder characterized by increased fibroblast proliferation and fibrotic tissue formation. These connections make PDGFR alpha a significant target for research and therapeutic intervention.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Tyrosine-protein kinase that acts as a cell-surface receptor for PDGFA, PDGFB and PDGFC and plays an essential role in the regulation of embryonic development, cell proliferation, survival and chemotaxis. Depending on the context, promotes or inhibits cell proliferation and cell migration. Plays an important role in the differentiation of bone marrow-derived mesenchymal stem cells. Required for normal skeleton development and cephalic closure during embryonic development. Required for normal development of the mucosa lining the gastrointestinal tract, and for recruitment of mesenchymal cells and normal development of intestinal villi. Plays a role in cell migration and chemotaxis in wound healing. Plays a role in platelet activation, secretion of agonists from platelet granules, and in thrombin-induced platelet aggregation. Binding of its cognate ligands - homodimeric PDGFA, homodimeric PDGFB, heterodimers formed by PDGFA and PDGFB or homodimeric PDGFC -leads to the activation of several signaling cascades; the response depends on the nature of the bound ligand and is modulated by the formation of heterodimers between PDGFRA and PDGFRB. Phosphorylates PIK3R1, PLCG1, and PTPN11. Activation of PLCG1 leads to the production of the cellular signaling molecules diacylglycerol and inositol 1,4,5-trisphosphate, mobilization of cytosolic Ca(2+) and the activation of protein kinase C. Phosphorylates PIK3R1, the regulatory subunit of phosphatidylinositol 3-kinase, and thereby mediates activation of the AKT1 signaling pathway. Mediates activation of HRAS and of the MAP kinases MAPK1/ERK2 and/or MAPK3/ERK1. Promotes activation of STAT family members STAT1, STAT3 and STAT5A and/or STAT5B. Receptor signaling is down-regulated by protein phosphatases that dephosphorylate the receptor and its down-stream effectors, and by rapid internalization of the activated receptor.

See full target information PDGFRA

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-PDGFR alpha antibody [BLR128H] - BSA free

Immunocytochemistry - Anti-PDGFR alpha antibody [BLR128H] - BSA free (AB314105)

Immunoprecipitation - Anti-PDGFR alpha antibody [BLR128H] - BSA free (AB314105)

Immunocytochemistry - Anti-PDGFR alpha antibody [BLR128H] - BSA free (AB314105)

Western blot - Anti-PDGFR alpha antibody [BLR128H] - BSA free (AB314105)

Western blot - Anti-PDGFR alpha antibody [BLR128H] - BSA free (AB314105)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Product details

Properties and storage information

Form

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Complementary Products

Assay Kits

Primary Antibodies

Secondary Antibodies

Primary Antibodies

Primary Antibodies

Product promise