Rat Monoclonal PDGFR alpha antibody. Suitable for IHC-P and reacts with Mouse samples. Cited in 4 publications. Immunogen corresponding to Recombinant Fragment Protein within Mouse Pdgfra.
Images
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PDGFR alpha antibody [RM0004-3G28] (AB51875), expandable thumbnail](https://content.abcam.com/products/images/pdgfr-alpha-antibody-rm0004-3g28-ab51875--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img82911.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PDGFR alpha antibody [RM0004-3G28] (AB51875)")
Publications
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- NPJ precision oncology 5:262021Progression of prostate carcinoma is promoted by adipose stromal cell-secreted CXCL12 signaling in prostate epithelium.Applications:Unspecified applicationReactive species:Unspecified reactive speciesFei Su,Alexes C Daquinag,Songyeon Ahn,Achinto Saha,Yulin Dai,Zhongming Zhao,John DiGiovanni,Mikhail G KoloninPubMed 33753872
- Development (Cambridge, England) 145:2018PDGFRα/PDGFRβ signaling balance modulates progenitor cell differentiation into white and beige adipocytes.Applications:Unspecified applicationReactive species:Unspecified reactive speciesZhanguo Gao et. al.PubMed 29158445
- Molecular therapy : the journal of the American Society of Gene Therapy 24:34-402015Targeted Proapoptotic Peptides Depleting Adipose Stromal Cells Inhibit Tumor Growth.Applications:Unspecified applicationReactive species:Unspecified reactive speciesAlexes C Daquinag,Chieh Tseng,Yan Zhang,Felipe Amaya-Manzanares,Fernando Florez,Ali Dadbin,Tao Zhang,Mikhail G KoloninPubMed 26316391
- Cell death and differentiation 22:351-632014Depletion of white adipocyte progenitors induces beige adipocyte differentiation and suppresses obesity development.Applications:Unspecified applicationReactive species:Unspecified reactive speciesA C Daquinag,C Tseng,A Salameh,Y Zhang,F Amaya-Manzanares,A Dadbin,F Florez,Y Xu,Q Tong,M G KoloninPubMed 25342467
Key facts
- Isotype
- IgG2a
- Host species
- Rat
- Storage buffer
Constituents: PBS
- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- Recombinant Fragment Protein within Mouse Pdgfra. The exact immunogen used to generate this antibody is proprietary information. Database link P26618
Reactivity data
Select an application| IHC-P | |
|---|---|
| Mouse | Tested |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info 1/50.00000 - 1/100.00000 | Notes Perform enzymatic antigen retrieval before commencing with IHC staining protocol. |
Associated Products
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Target data
Function
Tyrosine-protein kinase that acts as a cell-surface receptor for PDGFA, PDGFB and PDGFC and plays an essential role in the regulation of embryonic development, cell proliferation, survival and chemotaxis. Depending on the context, promotes or inhibits cell proliferation and cell migration. Plays an important role in the differentiation of bone marrow-derived mesenchymal stem cells. Required for normal skeleton development and cephalic closure during embryonic development. Required for normal development of the mucosa lining the gastrointestinal tract, and for recruitment of mesenchymal cells and normal development of intestinal villi. Plays a role in cell migration and chemotaxis in wound healing. Plays a role in platelet activation, secretion of agonists from platelet granules, and in thrombin-induced platelet aggregation. Binding of its cognate ligands - homodimeric PDGFA, homodimeric PDGFB, heterodimers formed by PDGFA and PDGFB or homodimeric PDGFC -leads to the activation of several signaling cascades; the response depends on the nature of the bound ligand and is modulated by the formation of heterodimers between PDGFRA and PDGFRB. Phosphorylates PIK3R1, PLCG1, and PTPN11. Activation of PLCG1 leads to the production of the cellular signaling molecules diacylglycerol and inositol 1,4,5-trisphosphate, mobilization of cytosolic Ca(2+) and the activation of protein kinase C. Phosphorylates PIK3R1, the regulatory subunit of phosphatidylinositol 3-kinase, and thereby mediates activation of the AKT1 signaling pathway. Mediates activation of HRAS and of the MAP kinases MAPK1/ERK2 and/or MAPK3/ERK1. Promotes activation of STAT family members STAT1, STAT3 and STAT5A and/or STAT5B. Receptor signaling is down-regulated by protein phosphatases that dephosphorylate the receptor and its down-stream effectors, and by rapid internalization of the activated receptor.
Alternative names
CD140a, Platelet-derived growth factor receptor alpha, PDGF-R-alpha, PDGFR-alpha, Alpha platelet-derived growth factor receptor, Alpha-type platelet-derived growth factor receptor, CD140 antigen-like family member A, Platelet-derived growth factor alpha receptor, Pdgfra
Recommended products
Rat Monoclonal PDGFR alpha antibody. Suitable for IHC-P and reacts with Mouse samples. Cited in 4 publications. Immunogen corresponding to Recombinant Fragment Protein within Mouse Pdgfra.
Key facts
- Isotype
- IgG2a
- Host species
- Rat
- Storage buffer
Constituents: PBS
- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- Recombinant Fragment Protein within Mouse Pdgfra. The exact immunogen used to generate this antibody is proprietary information. Database link P26618
- Clone number
- RM0004-3G28
- Purification technique
- Affinity purification Protein G
- Specificity
Does not bind to PDGF receptor beta
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
PDGFR alpha also known as platelet-derived growth factor receptor alpha is a cell surface receptor tyrosine kinase with a mass of approximately 123 kDa. It plays a mechanical role in transmitting signals across the cell membrane initiating cellular responses. PDGFR alpha is widely expressed in mesenchymal cells including fibroblasts and smooth muscle cells. Researchers frequently employ techniques such as PDGFRA IHC and PDGFRA staining to study the localization and expression levels in tissues.
- Biological function summary
PDGFR alpha mediates cell proliferation survival and differentiation especially in embryonic development and wound healing. The receptor often forms a complex by dimerizing with itself or with PDGFR beta to activate intracellular signaling cascades. This process is initiated upon ligand binding which triggers autophosphorylation on tyrosine residues. The receptor is also recognized in the detection of PDGFRA with assays such as PDGFRA ELISA.
- Pathways
PDGFR alpha significantly affects the MAPK and PI3K/AKT signaling pathways which regulate cellular growth and survival signals. Through these pathways PDGFR alpha interacts with related proteins like SHP-2 and PI3K contributing to signal transduction involved in cellular responses. This role links PDGFR alpha to important cellular processes via its kinase activity and signal integration.
- Associated diseases and disorders
PDGFR alpha is implicated in conditions such as gastrointestinal stromal tumors (GISTs) and idiopathic pulmonary fibrosis (IPF). It connects to proteins such as KIT in GISTs where mutations cause aberrant receptor signaling leading to tumorigenesis. Aberrant PDGFR alpha signaling also associates with IPF a disorder characterized by increased fibroblast proliferation and fibrotic tissue formation. These connections make PDGFR alpha a significant target for research and therapeutic intervention.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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1 product image
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PDGFR alpha antibody [RM0004-3G28] (ab51875), expandable thumbnail](https://content.abcam.com/products/images/pdgfr-alpha-antibody-rm0004-3g28-ab51875--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img82911.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PDGFR alpha antibody [RM0004-3G28] (ab51875)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PDGFR alpha antibody [RM0004-3G28] (ab51875)
ab51875 at 1/100 dilution, staining PDGF Receptor alpha in LPS exposed mouse pancreatic tissue section by Immunohistochemistry (Formalin/PFA fixed paraffin-embedded sections).
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