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Anti-PDGFR alpha (mutated D842V) antibody [EPR26304-280] is a Rabbit Monoclonal antibody that is used in PDGFR alpha (mutated D842V) Flow Cytometry (Intra), ICC/IF, IHC-P, IP, Western Blot. Suitable for Human samples.

PDGFRA is a receptor tyrosine kinase whose binding to the important family of platelet derived growth factors leads to activation of the PI3K/AKt pathway. Its importance in cancer is attested by the fact that all Gastrointstinal Stromal Tumors (GISTs) arise from activating mutations in either PDGFRA or c-KIT. Besides GISTS, PDGRA is well studied in neuronal cancers, lung cancers, breast cancers and osteosarcomas.

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Images

Western blot - Anti-PDGFR alpha (mutated D842V) antibody [EPR26304-280] (AB313771), expandable thumbnail
  • Flow Cytometry (Intracellular) - Anti-PDGFR alpha (mutated D842V) antibody [EPR26304-280] (AB313771), expandable thumbnail
  • Immunocytochemistry/ Immunofluorescence - Anti-PDGFR alpha (mutated D842V) antibody [EPR26304-280] (AB313771), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PDGFR alpha (mutated D842V) antibody [EPR26304-280] (AB313771), expandable thumbnail
  • Immunoprecipitation - Anti-PDGFR alpha (mutated D842V) antibody [EPR26304-280] (AB313771), expandable thumbnail

Key facts

Isotype
IgG
Host species
Rabbit
Storage buffer

pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Form
Liquid
Clonality
Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
WBIHC-PICC/IFFlow Cyt (Intra)IP
Human
Predicted
Predicted
Predicted
Predicted
Predicted
Transfected cell line - Human
Not recommended
Tested
Tested
Tested
Not recommended
Transfected cell lysate - Human
Tested
Not recommended
Not recommended
Not recommended
Tested

Tested
Tested

Species
Transfected cell lysate - Human
Dilution info
1/1000
Notes

-

Predicted
Predicted

Species
Human
Dilution info
-
Notes

-

Not recommended
Not recommended

Species
Transfected cell line - Human
Dilution info
-
Notes

-

Tested
Tested

Species
Transfected cell line - Human
Dilution info
1/4000
Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Predicted
Predicted

Species
Human
Dilution info
-
Notes

-

Not recommended
Not recommended

Species
Transfected cell lysate - Human
Dilution info
-
Notes

-

Tested
Tested

Species
Transfected cell line - Human
Dilution info
1/500
Notes

-

Predicted
Predicted

Species
Human
Dilution info
-
Notes

-

Not recommended
Not recommended

Species
Transfected cell lysate - Human
Dilution info
-
Notes

-

Tested
Tested

Species
Transfected cell line - Human
Dilution info
1/50
Notes

-

Predicted
Predicted

Species
Human
Dilution info
-
Notes

-

Not recommended
Not recommended

Species
Transfected cell lysate - Human
Dilution info
-
Notes

-

Tested
Tested

Species
Transfected cell lysate - Human
Dilution info
1/30
Notes

-

Predicted
Predicted

Species
Human
Dilution info
-
Notes

-

Not recommended
Not recommended

Species
Transfected cell line - Human
Dilution info
-
Notes

-

Associated Products

Select an associated product type

1 product for Alternative Version

Target data

Function

Tyrosine-protein kinase that acts as a cell-surface receptor for PDGFA, PDGFB and PDGFC and plays an essential role in the regulation of embryonic development, cell proliferation, survival and chemotaxis. Depending on the context, promotes or inhibits cell proliferation and cell migration. Plays an important role in the differentiation of bone marrow-derived mesenchymal stem cells. Required for normal skeleton development and cephalic closure during embryonic development. Required for normal development of the mucosa lining the gastrointestinal tract, and for recruitment of mesenchymal cells and normal development of intestinal villi. Plays a role in cell migration and chemotaxis in wound healing. Plays a role in platelet activation, secretion of agonists from platelet granules, and in thrombin-induced platelet aggregation. Binding of its cognate ligands - homodimeric PDGFA, homodimeric PDGFB, heterodimers formed by PDGFA and PDGFB or homodimeric PDGFC -leads to the activation of several signaling cascades; the response depends on the nature of the bound ligand and is modulated by the formation of heterodimers between PDGFRA and PDGFRB. Phosphorylates PIK3R1, PLCG1, and PTPN11. Activation of PLCG1 leads to the production of the cellular signaling molecules diacylglycerol and inositol 1,4,5-trisphosphate, mobilization of cytosolic Ca(2+) and the activation of protein kinase C. Phosphorylates PIK3R1, the regulatory subunit of phosphatidylinositol 3-kinase, and thereby mediates activation of the AKT1 signaling pathway. Mediates activation of HRAS and of the MAP kinases MAPK1/ERK2 and/or MAPK3/ERK1. Promotes activation of STAT family members STAT1, STAT3 and STAT5A and/or STAT5B. Receptor signaling is down-regulated by protein phosphatases that dephosphorylate the receptor and its down-stream effectors, and by rapid internalization of the activated receptor.

Alternative names

CD140a, PDGFR2, RHEPDGFRA, PDGFRA, Platelet-derived growth factor receptor alpha, PDGF-R-alpha, PDGFR-alpha, Alpha platelet-derived growth factor receptor, Alpha-type platelet-derived growth factor receptor, CD140 antigen-like family member A, CD140a antigen, Platelet-derived growth factor alpha receptor, Platelet-derived growth factor receptor 2, PDGFR-2

Recommended products

Anti-PDGFR alpha (mutated D842V) antibody [EPR26304-280] is a Rabbit Monoclonal antibody that is used in PDGFR alpha (mutated D842V) Flow Cytometry (Intra), ICC/IF, IHC-P, IP, Western Blot. Suitable for Human samples.

PDGFRA is a receptor tyrosine kinase whose binding to the important family of platelet derived growth factors leads to activation of the PI3K/AKt pathway. Its importance in cancer is attested by the fact that all Gastrointstinal Stromal Tumors (GISTs) arise from activating mutations in either PDGFRA or c-KIT. Besides GISTS, PDGRA is well studied in neuronal cancers, lung cancers, breast cancers and osteosarcomas.

Key facts

Isotype
IgG
Form
Liquid
Clonality
Monoclonal
Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Clone number
EPR26304-280
Purification technique
Affinity purification Protein A
Specificity

This antibody has not been validated in endogenous samples.

Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle

Notes

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.
Activity summary

PDGFR alpha also known as platelet-derived growth factor receptor alpha is a cell surface receptor tyrosine kinase with a mass of approximately 123 kDa. It plays a mechanical role in transmitting signals across the cell membrane initiating cellular responses. PDGFR alpha is widely expressed in mesenchymal cells including fibroblasts and smooth muscle cells. Researchers frequently employ techniques such as PDGFRA IHC and PDGFRA staining to study the localization and expression levels in tissues.

Biological function summary

PDGFR alpha mediates cell proliferation survival and differentiation especially in embryonic development and wound healing. The receptor often forms a complex by dimerizing with itself or with PDGFR beta to activate intracellular signaling cascades. This process is initiated upon ligand binding which triggers autophosphorylation on tyrosine residues. The receptor is also recognized in the detection of PDGFRA with assays such as PDGFRA ELISA.

Pathways

PDGFR alpha significantly affects the MAPK and PI3K/AKT signaling pathways which regulate cellular growth and survival signals. Through these pathways PDGFR alpha interacts with related proteins like SHP-2 and PI3K contributing to signal transduction involved in cellular responses. This role links PDGFR alpha to important cellular processes via its kinase activity and signal integration.

Associated diseases and disorders

PDGFR alpha is implicated in conditions such as gastrointestinal stromal tumors (GISTs) and idiopathic pulmonary fibrosis (IPF). It connects to proteins such as KIT in GISTs where mutations cause aberrant receptor signaling leading to tumorigenesis. Aberrant PDGFR alpha signaling also associates with IPF a disorder characterized by increased fibroblast proliferation and fibrotic tissue formation. These connections make PDGFR alpha a significant target for research and therapeutic intervention.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

7 product images

  • Western blot - Anti-PDGFR alpha (mutated D842V) antibody [EPR26304-280] (ab313771), expandable thumbnail

    Western blot - Anti-PDGFR alpha (mutated D842V) antibody [EPR26304-280] (ab313771)

    PDGFR alpha (mutated D842V) Western blot staining using rabbit Anti-PDGFR alpha (mutated D842V) antibody

    Blocking and diluting buffer and concentration: 5% NFDM/TBST
    This antibody recognizes human PDGFR (D842V) but not human wild-type PDGFRA. In western blot, anti-GAPDH antibody [EPR16891] - Loading Control (Anti-GAPDH antibody [EPR16891] - Loading Control ab181602) staining at 1/200000 dilution and anti-PDGFRA antibody (Anti-PDGFR alpha antibody [EPR22059-270] ab203491) staining at 1/1000 dilution. A-172 cells are negative for wild-type PDGFRA (PMID:8425771).

    All lanes: Western blot - Anti-PDGFR alpha (mutated D842V) antibody [EPR26304-280] (ab313771) at 1/1000 dilution

    Lane 1: 293T cells transfected with an empty vector containing a His-tag, whole cell lysate at 20 µg

    Lane 2: 293T cells transfected with a human wild-type PDGFRA expression vector containing a His-tag, whole cell lysate at 2 µg

    Lane 3: 293T cells transfected with a human PDGFRA (D842V) expression vector containing a His-tag, whole cell lysate at 2 µg

    Lane 4: 293T cells transfected with a human PDGFRA (D842V) expression vector containing a His-tag, whole cell lysate at 0.4 µg

    Lane 5: MG-63 (human osteosarcoma fibroblast) whole cell lysate at 20 µg

    Lane 6: A-204 (human muscle rhabdomyosarcoma cell) whole cell lysate at 20 µg

    Lane 7: A-172 (human brain glioblastoma ) whole cell lysate at 20 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution

    Observed band size: 160 kDa

    Exposure time: 180s

  • Flow Cytometry (Intracellular) - Anti-PDGFR alpha (mutated D842V) antibody [EPR26304-280] (ab313771), expandable thumbnail

    Flow Cytometry (Intracellular) - Anti-PDGFR alpha (mutated D842V) antibody [EPR26304-280] (ab313771)

    Flow cytometric analysis of 293T (human embryonic kidney epithelial cell) transfected with a human wild-type PDGFRA expression vector containing a Myc tag (left); human PDGFRA (D842V) expression vector containing a Myc tag (right). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. Cell labelling of PDGFRA (D842V) with ab313771 at 1/50 dilution. A Goat Anti-Rabbit IgG (Alexa Fluor® 488, Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081) was used at 1/5000 dilution. No cross-reactivity with human wild-type PDGFRA.

  • Immunocytochemistry/ Immunofluorescence - Anti-PDGFR alpha (mutated D842V) antibody [EPR26304-280] (ab313771), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Anti-PDGFR alpha (mutated D842V) antibody [EPR26304-280] (ab313771)

    Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized 293T (human embryonic kidney epithelial cell) labelling PDGFRA (D842V) with ab313771 at 1/500 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed antibody at 1/1000 dilution (Green). Cells were counterstained with anti-Myc tag Mouse mab [9E10] (Alexa Fluor® 594) at 1/100 dilution (Red). The Nuclear counterstain is DAPI (Blue).

    Confocal image showing cytoplasmic and membrane staining in 293T overexpressing human PDGFRA (D842V). No staining in 293T cells overexpressing human wild-type PDGFRA. Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

    Secondary antibody only control: Secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 (2 µg/mL) dilution.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PDGFR alpha (mutated D842V) antibody [EPR26304-280] (ab313771), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PDGFR alpha (mutated D842V) antibody [EPR26304-280] (ab313771)

    Immunohistochemical analysis of paraffin-embedded (A, D) human endometrial tissue; (B, E) human placenta; (C, F) human colon carcinoma.

    A-C: Sections were incubated with anti-PDGFRA [EPR22059-270] (Anti-PDGFR alpha antibody [EPR22059-270] ab203491; 1:2000 [0.28µg/mL]) for 30 mins at room temperature. Positive staining on human endometrium (image A), placenta (image B), and colon carcinoma (image C). D-F: Sections were incubated with anti-PDGFRA D842V [EPR26304-280] (ab313771; 1:4000 [0.13µg/mL]) for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument counterstained with Hematoxylin. No staining on human endometrium (image D), placenta (image E), or colon carcinoma (image F). No cross-reactivity with human wild-type PDGFRA.

    Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

    Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.

  • Immunoprecipitation - Anti-PDGFR alpha (mutated D842V) antibody [EPR26304-280] (ab313771), expandable thumbnail

    Immunoprecipitation - Anti-PDGFR alpha (mutated D842V) antibody [EPR26304-280] (ab313771)

    PDGFRA (D842V) was immunoprecipitated from 0.35 mg of 293T (human embryonic kidney epithelial cell) transfected with a human PDGFRA (D842V) expression vector containing a His tag using ab313771 at 1/30 dilution (2µg in 0.35mg whole cell lysate). Western blot was performed on the immunoprecipitate using ab313771 at 1/1000 dilution. VeriBlot for IP secondary antibody (HRP)(VeriBlot for IP Detection Reagent (HRP) ab131366) was used at 1/5000 dilution. Isotype control - Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730).
    Lanes 1-3: 293T (human embryonic kidney epithelial cell) transfected with a human PDGFRA (D842V) expression vector containing a His tag
    Lane 1: whole cell lysate at 2µg
    Lane 2: IP with ab313771
    Lane 3: IP with Isotype control (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730)
    Blocking and dilution buffer and concentration: 5% NFDM/TBST.

    Exposure time: 24 seconds

    All lanes: Immunoprecipitation - Anti-PDGFR alpha (mutated D842V) antibody [EPR26304-280] (ab313771) at 1/30 dilution

    All lanes: 293T cells transfected with a human PDGFRA (D842V) expression vector containing a His-tag whole cell lysate

    Secondary

    All lanes: Immunoprecipitation - VeriBlot for IP Detection Reagent (HRP) (VeriBlot for IP Detection Reagent (HRP) ab131366) at 1/5000 dilution

    Exposure time: 24s

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PDGFR alpha (mutated D842V) antibody [EPR26304-280] (ab313771), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PDGFR alpha (mutated D842V) antibody [EPR26304-280] (ab313771)

    Immunohistochemical analysis of paraffin-embedded 293T (human embryonic kidney epithelial cell) transfected with (A & D) human PDGFRA (D842V) expression vector containing a His tag; (B & E) human wild-type PDGFRA expression vector containing a His tag; (C & F) empty vector containing a His tag.
    A-C: whole cell pellet labelling of human wild-type and mutated PDGFRA with Anti-PDGFR alpha antibody [EPR22059-270] ab203491 at 1/2000 dilution (0.28 µg/ml). D-F: whole cell pellet labelling of human PDGFRA (D842V) with ab313771 at 1/4000 dilution (0.13 µg/ml). Sections were incubated with ab203491or ab313771 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument counterstained with Hematoxylin
    This antibody recognizes human PDGFR (D842V) but not human wild-type PDGFRA. Positive staining on 293T overexpressing human PDGFR (D842V) (image D). No staining on 293T overexpressing human wild-type PDGFRA (image E) or the empty vector control (image F).
    Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
    Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PDGFR alpha (mutated D842V) antibody [EPR26304-280] (ab313771), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PDGFR alpha (mutated D842V) antibody [EPR26304-280] (ab313771)

    Immunohistochemical analysis of paraffin-embedded Human stomach tissue labeling PDGFR alpha (mutated D842V) with ab313771 at 1/4000 (0.13 ug/ml) followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Negative control: No staining was observed in all normal tissues tested. The section was incubated with ab313771 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

    Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.

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Product protocols

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