Rabbit Monoclonal PDGFR beta antibody. Carrier free. Suitable for IP, ELISA, WB, IHC-P, IHC-FoFr, ICC/IF, Flow Cyt (Intra) and reacts with Mouse, Human samples.
IgG
Rabbit
pH: 7.2 - 7.4
Constituents: PBS
Liquid
Monoclonal
IP | ELISA | WB | IHC-P | IHC-FoFr | ICC/IF | Flow Cyt (Intra) | |
---|---|---|---|---|---|---|---|
Human | Expected | Tested | Tested | Tested | Expected | Expected | Expected |
Mouse | Tested | Expected | Expected | Expected | Expected | Expected | Tested |
Rat | Predicted | Predicted | Predicted | Predicted | Predicted | Predicted | Predicted |
Species | Dilution info | Notes |
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Species Mouse | Dilution info - | Notes - |
Species | Dilution info | Notes |
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Species Human | Dilution info Use at an assay dependent concentration. | Notes - |
Species | Dilution info | Notes |
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Species Rat | Dilution info - | Notes - |
Species | Dilution info | Notes |
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Species Human | Dilution info - | Notes - |
Species | Dilution info | Notes |
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Species Mouse | Dilution info Use at an assay dependent concentration. | Notes - |
Species | Dilution info | Notes |
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Species Rat | Dilution info - | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Human | Dilution info - | Notes For samples expressing low levels of PDGFR beta, the ;amount of lysate loaded ;may need to be increased to allow detection. ; |
Species | Dilution info | Notes |
---|---|---|
Species Mouse | Dilution info Use at an assay dependent concentration. | Notes - |
Species | Dilution info | Notes |
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Species Rat | Dilution info - | Notes - |
Species | Dilution info | Notes |
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Species Human | Dilution info - | Notes See IHC antigen retrieval protocols.Optimisation of the IHC protocol may be required depending on the sample used. |
Species | Dilution info | Notes |
---|---|---|
Species Mouse | Dilution info Use at an assay dependent concentration. | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Rat | Dilution info - | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Human, Mouse | Dilution info Use at an assay dependent concentration. | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Rat | Dilution info - | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Human, Mouse | Dilution info Use at an assay dependent concentration. | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Rat | Dilution info - | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Mouse | Dilution info - | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Human | Dilution info Use at an assay dependent concentration. | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Rat | Dilution info - | Notes - |
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Tyrosine-protein kinase that acts as cell-surface receptor for homodimeric PDGFB and PDGFD and for heterodimers formed by PDGFA and PDGFB, and plays an essential role in the regulation of embryonic development, cell proliferation, survival, differentiation, chemotaxis and migration. Plays an essential role in blood vessel development by promoting proliferation, migration and recruitment of pericytes and smooth muscle cells to endothelial cells. Plays a role in the migration of vascular smooth muscle cells and the formation of neointima at vascular injury sites. Required for normal development of the cardiovascular system. Required for normal recruitment of pericytes (mesangial cells) in the kidney glomerulus, and for normal formation of a branched network of capillaries in kidney glomeruli. Promotes rearrangement of the actin cytoskeleton and the formation of membrane ruffles. Binding of its cognate ligands - homodimeric PDGFB, heterodimers formed by PDGFA and PDGFB or homodimeric PDGFD -leads to the activation of several signaling cascades; the response depends on the nature of the bound ligand and is modulated by the formation of heterodimers between PDGFRA and PDGFRB. Phosphorylates PLCG1, PIK3R1, PTPN11, RASA1/GAP, CBL, SHC1 and NCK1. Activation of PLCG1 leads to the production of the cellular signaling molecules diacylglycerol and inositol 1,4,5-trisphosphate, mobilization of cytosolic Ca(2+) and the activation of protein kinase C. Phosphorylation of PIK3R1, the regulatory subunit of phosphatidylinositol 3-kinase, leads to the activation of the AKT1 signaling pathway. Phosphorylation of SHC1, or of the C-terminus of PTPN11, creates a binding site for GRB2, resulting in the activation of HRAS, RAF1 and down-stream MAP kinases, including MAPK1/ERK2 and/or MAPK3/ERK1. Promotes phosphorylation and activation of SRC family kinases. Promotes phosphorylation of PDCD6IP/ALIX and STAM. Receptor signaling is down-regulated by protein phosphatases that dephosphorylate the receptor and its down-stream effectors, and by rapid internalization of the activated receptor.
PDGFRA
Platelet-derived growth factor receptor beta, PDGF-R-beta, PDGFR-beta, Beta platelet-derived growth factor receptor, Beta-type platelet-derived growth factor receptor, CD140 antigen-like family member B, Platelet-derived growth factor receptor 1, PDGFR-1, PDGFRB, PDGFR, PDGFR1
Rabbit Monoclonal PDGFR beta antibody. Carrier free. Suitable for IP, ELISA, WB, IHC-P, IHC-FoFr, ICC/IF, Flow Cyt (Intra) and reacts with Mouse, Human samples.
Platelet-derived growth factor receptor beta, PDGF-R-beta, PDGFR-beta, Beta platelet-derived growth factor receptor, Beta-type platelet-derived growth factor receptor, CD140 antigen-like family member B, Platelet-derived growth factor receptor 1, PDGFR-1, PDGFRB, PDGFR, PDGFR1
IgG
Rabbit
pH: 7.2 - 7.4
Constituents: PBS
Liquid
Monoclonal
Yes
Y92
Affinity purification Protein A
Expression levels of the target protein vary with sample type and some optimisation may be required.
Blue Ice
+4°C
Do Not Freeze
ab271835 is the carrier-free version of Anti-PDGFR alpha + PDGFR beta antibody [Y92] - C-terminal ab32570.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
For more information, read more on recombinant antibodies.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
This supplementary information is collated from multiple sources and compiled automatically.
PDGFR alpha and PDGFR beta are receptor tyrosine kinases also known as CD140a and CD140b. Both receptors have a molecular mass of about 180 kDa. PDGFR alpha is expressed in a variety of tissues including placenta astrocytes and vascular smooth muscle cells. PDGFR beta is more specific to fibroblasts smooth muscle cells and in the vascular system. These receptors bind platelet-derived growth factors (PDGFs) and become activated through dimerization and autophosphorylation.
These receptors drive cellular processes like proliferation differentiation and migration. PDGFR alpha and beta operate as a significant part of a receptor complex. They modulate responses in mesenchymal cells and influence developmental pathways. In the vasculature system these receptors play roles in maintaining structure and function of blood vessels. Alterations in receptor activities can affect development of tissues and organs.
PDGFR alpha and beta are key players in the PI3K-Akt signaling pathway and MAPK pathway. The activation of these pathways leads to cellular processes like survival and growth. PDGFR activity often interacts with other proteins such as SHP-2 RAS and Akt. These interactions contribute to the regulation of cellular responses to external growth signals embedding PDGFR in a web of intracellular cascade systems.
PDGFRs have linkage to conditions like cancer and fibrotic diseases. Aberrant PDGFR alpha activity has connections to glioblastoma while PDGFR beta alterations often relate to systemic sclerosis. These receptors can work alongside proteins like VEGF and TGF-beta within these diseases. Their dysregulation leads to pathological angiogenesis and abnormal cell proliferation making them targets for therapeutic intervention.
We have tested this species and application combination and it works. It is covered by our product promise.
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-PDGFR alpha + PDGFR beta antibody [Y92] - C-terminal ab32570).
Blocking/Diluting buffer and concentration: 5% NFDM/TBST
All lanes: Western blot - Anti-PDGFR alpha + PDGFR beta antibody [Y92] - C-terminal (Anti-PDGFR alpha + PDGFR beta antibody [Y92] - C-terminal ab32570) at 1/1000 dilution
Lane 1: Western blot - Recombinant human PDGFR beta protein (Recombinant human PDGFR beta protein ab60833) at 0.1 µg
Lane 2: Western blot - Recombinant human PDGFR alpha protein (Recombinant human PDGFR alpha protein ab84797) at 0.1 µg
All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution
Exposure time: 3s
Blocking/Diluting buffer and concentration: 5% NFDM/TBST
This data was developed using the same antibody clone in a different buffer formulation (Anti-PDGFR alpha + PDGFR beta antibody [Y92] - C-terminal ab32570).
Lanes 1 - 4: Merged signal (red and green). Green - Anti-PDGFR alpha + PDGFR beta antibody [Y92] - C-terminal ab32570 observed at 170 kDa. Red - loading control Anti-alpha Tubulin antibody [DM1A] - Loading Control ab7291 (Mouse anti-Alpha Tubulin [DM1A]) observed at 55 kDa.
Anti-PDGFR alpha + PDGFR beta antibody [Y92] - C-terminal ab32570 was shown to react with PDGFRB in wild-type SH-SY5Y cells in Western blot with loss of signal observed in PDGFRB knockout cell line Human PDGFRB knockout SH-SY5Y cell line ab273749 (knockout cell lysate Human PDGFRB knockout SH-SY5Y cell lysate ab275523). Wild-type SH-SY5Y and PDGFRB knockout cell lysates were subjected to SDS-PAGE. Membranes were blocked in fluorescent western blot (TBS-based) blocking solution before incubation with Anti-PDGFR alpha + PDGFR beta antibody [Y92] - C-terminal ab32570 and Anti-alpha Tubulin antibody [DM1A] - Loading Control ab7291 (Mouse anti-Alpha Tubulin [DM1A]) overnight at 4 °C at a 1 in 5000 dilution and a 1 in 20000 dilution respectively. Blots were incubated with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed (Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed (Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed ab216776) secondary antibodies at 1 in 20000 dilution for 1 h at room temperature before imaging.
All lanes: Western blot - Anti-PDGFR alpha + PDGFR beta antibody [Y92] - C-terminal (Anti-PDGFR alpha + PDGFR beta antibody [Y92] - C-terminal ab32570) at 1/5000 dilution
Lane 1: Wild-type SH-SY5Y cell lysate at 30 µg
Lane 2: PDGFR beta knockout SH-SY5Y cell lysate at 30 µg
Lane 3: Human Skeletal Muscle tissue lysate at 30 µg
Lane 4: HeLa cell lysate at 30 µg
Performed under reducing conditions.
Predicted band size: 123 kDa
Observed band size: 170 kDa
Anti-PDGFR alpha + PDGFR beta antibody [Y92] - C-terminal ab32570 (purified) at 1/20 immunoprecipitating PDGFR alpha + beta in NIH/3T3 (Mouse embryo fibroblast cell line) (Lane 1 and 2). Lane 3 - PBS.
For western blotting a HRP-conjugated anti-rabbit IgG specific to the non-reduced form of IgG was used as the secondary antibody (1/1500).
Blocking buffer and concentration: 5% NFDM/TBST.
Diluting buffer and concentration: 5% NFDM /TBST.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-PDGFR alpha + PDGFR beta antibody [Y92] - C-terminal ab32570).
All lanes: Immunoprecipitation - Anti-PDGFR alpha + PDGFR beta antibody [Y92] - C-terminal (Anti-PDGFR alpha + PDGFR beta antibody [Y92] - C-terminal ab32570)
Observed band size: 175 kDa
Anti-PDGFR alpha + PDGFR beta antibody [Y92] - C-terminal ab32570 staining PDGFR alpha + beta in human breast tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Shows positive staining on stromal cells. Heat mediated antigen retrieval was performed using Antigen Retrieval Buffer (100X Tris-EDTA Buffer, pH 9.0) ab93684 (Tris/EDTA buffer, pH 9.0). Samples were incubated with primary antibody (1/500). An HRP-conjugated Goat anti-rabbit IgG (ready to use) was used as the secondary antibody. Counter stained with Hematoxylin.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-PDGFR alpha + PDGFR beta antibody [Y92] - C-terminal ab32570).
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-PDGFR alpha + PDGFR beta antibody [Y92] - C-terminal ab32570).
ELISA showing primary antibody Anti-PDGFR alpha + PDGFR beta antibody [Y92] - C-terminal ab32570 binding to the antigen Human PDGFR alpha protein and Human PDGFR beta protein.
Primary antibody concentration ranges from 0 - 1000 ng/mL.
Alkaline Phosphatase-conjugated AffiniPure Goat Anti-Rabbit IgG (H+L) was used as a secondary antibody at 1/2500 dilution.
This data was developed using Anti-PDGFR alpha + PDGFR beta antibody [Y92] - C-terminal ab32570, the same antibody clone in a different buffer formulation. Different batches of Anti-PDGFR alpha + PDGFR beta antibody [Y92] - C-terminal ab32570 were tested on Rat brain lysate at 1.0 µg/ml. 15 µg of lysate was loaded in each lane. Bands observed at 175 kDa.
All lanes: Western blot - Anti-PDGFR alpha + PDGFR beta antibody [Y92] - C-terminal (Anti-PDGFR alpha + PDGFR beta antibody [Y92] - C-terminal ab32570)
Anti-PDGFR alpha + PDGFR beta antibody [Y92] - C-terminal ab32570 staining PDGFR alpha + beta in human lung cancer tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Shows positive staining on stromal cells. Heat mediated antigen retrieval was performed using Antigen Retrieval Buffer (100X Tris-EDTA Buffer, pH 9.0) ab93684 (Tris/EDTA buffer, pH 9.0). Samples were incubated with primary antibody (1/500). An HRP-conjugated Goat anti-rabbit IgG (ready to use) was used as the secondary antibody. Counter stained with Hematoxylin.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-PDGFR alpha + PDGFR beta antibody [Y92] - C-terminal ab32570).
Immunohistochemical staining of paraffin embedded human spleen with purified Anti-PDGFR alpha + PDGFR beta antibody [Y92] - C-terminal ab32570 at a working dilution of 1/50. The secondary antibody used is Goat Anti-Rabbit IgG H&L (HRP) ab97051, a HRP-conjugated goat anti-rabbit IgG (H+L), at a dilution of 1/500. The sample is counter-stained with hematoxylin. Antigen retrieval was perfomed using Tris-EDTA buffer, pH 9.0. PBS was used instead of the primary antibody as the negative control, and is shown in the inset.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-PDGFR alpha + PDGFR beta antibody [Y92] - C-terminal ab32570).
Intracellular Flow Cytometry analysis of NIH/3T3 (Mouse embryo fibroblast cell line) cells labeling PDGFR alpha + beta (red) with Anti-PDGFR alpha + PDGFR beta antibody [Y92] - C-terminal ab32570 at a 1/20 dilution. Cells were fixed with 4% paraformaldehyde and permeabilized with 90% methanol. A goat anti-rabbit IgG (Alexa Fluor® 488) (Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) was used as the secondary antibody at a 1/2000 dilution. Black - Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730). Blue (unlabeled control) - Cells without incubation with primary and secondary antibodies.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-PDGFR alpha + PDGFR beta antibody [Y92] - C-terminal ab32570).
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